研究生: |
楊盈盈 Yang Ying-Ying |
---|---|
論文名稱: |
線上掃集濃縮-毛細管微胞電動層析法對靶場土壤中火炸藥之分析研究 Determination of nitroaromatic explosives residue at military shooting ranges using a sweeping-MEKC method |
指導教授: |
林震煌
Lin, Cheng-Huang |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2008 |
畢業學年度: | 96 |
語文別: | 中文 |
中文關鍵詞: | 火炸藥 、土壤 、三硝基甲苯 |
英文關鍵詞: | TNT, RDX, soil, sweeping-MEKC, EPA Method 8330, explosive |
論文種類: | 學術論文 |
相關次數: | 點閱:167 下載:0 |
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本研究成功的利用毛細管電泳/紫外光吸收偵測法 (CE/UV),結合線上濃縮的技術,分析多處靶場土壤中所殘留的火炸藥含量。實驗中,以微胞電動層析法(MEKC) 分析14種硝基芳香族及硝基胺類的火炸藥標準品:HMX、RDX、TNT、Tetryl、2,4-DNT、 2,6-DNT、2-NT、3-NT、4-NT、1,3,5-TNB、1,3-DNB、NB、4-Am-4,6-DNT、 4-AM-2,6-DNT等;MEKC模式的偵測極限 (LODs; S/N = 3) 為1.5 ~ 2.9 μg/mL,為提升偵測靈敏度,利用毛細管電泳掃集法 (sweeping-MEKC) 模式進行偵測,偵測極限可以提升到3.1 ~ 6.5 ng/mL。在真實樣品分析中,檢測金門七處不同靶場土壤之表層土,經過簡單的萃取步驟,在土壤中發現高濃度的 TNT及RDX等二種軍方最常使用的火炸藥。實驗中所開發之分析技術,有助於檢測土壤中殘存的火炸藥,並且可以作為未來相關研究的參考
This paper describes the process by which nitroaromatic explosives residue in soil sample matrices from military shooting ranges is obtained by means of capillary electrophoresis/UV absorbance. The use of MEKC (micellar electrokinetic chromatography) mode provided the limit of detections (LODs; at S/N = 3) ranging 1.5 ~ 2.9 μg/mL for 14 nitroaromatic and nitramine explosives standards; whereas when the sweeping-MEKC technique was used, the LODs could be improved as much as 3.1 ~ 6.5 ng/mL. A set of 7 soil samples were collected from surface soil at military shooting ranges located at Kinmen County in Taiwan, and the findings showed that RDX and TNT were the explosives residue present at the highest concentrations. This study is useful for determining current levels of explosives residue and to help with appropriate recommendations concerning future site characterization techniques.
[1] Chang, P. C., Yeh, T. F., Yuan, C. L., WHAMPOA - An Interdisciplinary, 2007, 52, 71-90
[2] Quirino, J. P., Terabe, S., Science 1998, 282, 465-468.
[3] Quirino, J. P., Terabe, S., Anal. Chem. 1999, 71, 1638-1644.
[4] Quirino, J. P., Kim, J.-B., Terabe, S., J. Chromatogr. A 2002, 965, 357-373.
[5] Batlle, R., Carllsson, H., Holmgren, E., ColmsjÖ, A., Crescnzi, C., Journal of Chromatography A, 2002, 963, 73-82
[6] Nam, S. I., Leggett, D. C., Jenkins, T. F., Stutz, M. H., AmericanEnvironmental Laboratory, 2000, 2, 4-7.
[7] Mares, B., Pokorny, J., Dvorak, P. Analysis of post-blast traces of explosives by TLC in model conditions.Proceedings from 6th InternationalSymposium on Analysis and Detectionof Explosives 1998; July 6-10: Prague,Czech Republic.
[8] Krishnamurthy, R., Bakre, S. M., Shinde, B. M., S. Journal of PlanarChromatography, 1999, 12, 394-397.
[9] Hannigan T. Thin layerchromatography (TLC). Proceedings from Workshop on Explosives TraceAnalysis Methods 1999; April 28-29:Forensic Explosives Laboratory, DERA,United Kingdom.
[10] Ewing, R. G., Atkinsom, D. A., Eicernan, G. A., Ewing, G. J., Talanta, 2001, 54, 515-529.
[11] Jiménez, A. M., Navas, M. J., Journal of Hazardous Materials, 2004, 106A,1-8.
[12] J.C. Spain, in: J.C. Spain, J.B. Hughes, H.-J. Knackmuss (Eds.), Biodegradation of Nitroaromatic Compounds and Explosives, CRC Press, Boca Raton FL, 2002, p. 1, Chapter 1.
[13] Hundal, L. S., Singh, J., Bier, E. L., Shea, P. J., Comfort, S. D., Powers, W. L., Environmental Pollution. 1997, 97, 55-64.
[14] Walsh, M. E., Talanta. 2001, 54, 427-438.
[15] Perr, J. M., Furton, K.G., Almirall, J. R., Talanta. 2005, 67, 430-436.
[16] Smedts, B. R., Baeyens, W., De Bisschop, H. C., Analytica Chimica Acta .2003, 495, 239-247
[17] Harper, R. J., Almirall, J. R., Furtom, K. G., Talanta. 2005, 67, 313-327.
[18] Halasz, A., Groom, C., Zhou, E., Paquet, L., Beaulieu, C., Deschamps, S., Corriveau, A., Thiboutot, S., Ampleman, G., Dubois, C., Hawari, J., Journal of Chromatography 2002, 963, 411-418.
[19] Hodyss, R., Beauchamp, J. L., Anal. Chem. 2005, 77, 3607-3610
[20] Pan, X., Zhang, B., Cobb, G. P., Talanta 2005, 67, 816-823.
[21] Yinon, J., Yost, R. A., Bulusu, A., Journal of Chromatography 1994, 688, 231-242.
[22] Marple, R. L., LaCourse, W. R., Talanta 2005, 66, 581-590.
[23] Lang, M. J., Burns, S. E., Journal of Chromatography 1999, 849, 381-388.
[24] EPA Method 8330, Environmental Protection Agency, Government of the United States.
[25] Giordano, B., C., Copper, C., L., Collins,G.,E., Electrophoresis 2006, 27, 778-786.
[26] Groom, C. A., Halasz, A., Paquet, L., Thiboutot, S., Ampleman, G., Hawari, J., Journal of Chromatography A. 2005, 1072, 73-82.
[27] Wallenborg, S. R., Bailey, C. G., Anal. Chem. 2000, 72, 1872-1878.
[28] Kennedy, S., Caddy, B., Douse, J. M. F., Journal of Chromatography 1996, 726, 211-222.
[29] Bailey, C. G., Yan, C., Anal. Chem. 1998, 70, 3275-3279.
[30] Wang, J., Pumera, M., Chatrathi, M. p., Escarpa, A., Musameh, M., Collins, G., Mulahandani, A., Lin,Y., Olsen, K., Anal. Chem. 2002, 74, 1187-1191.
[31] Burgi, D. S., Chien, R. L., Anal. Chem. 1991, 63, 2042-2047.
[32] Chien, R. L., Burgi, D. S., Anal. Chem. 1992, 64, 1046-1050.
[33] Chien, R. L., Burgi, D. S., Anal. Chem. 1992, 64, 489A-496A.
[34] He, Y., Lee, H. K., Anal. Chem. 1999, 71, 995-1001.
[35] Quirino, J. P., Terabe, S., J. Chromatogr. A 1999, 850, 339-344.
[36] Liu, W., Lee, H.-K., Electrophoresis 1999, 20, 2475-2483.
[37] Zhao, Y., Lunte, C. E., Anal. Chem. 1999, 71, 3985-3991.
[38] Quirino, J. P., Terabe, S., J. Chromatogr. A, 1999, 850, 339-344.
[39] Palmer, J., Munro, N. J., Landers, J. P., Anal. Chem.1999, 71, 1679-1687.
[40] Quirino, J. P., Terabe, S., Anal. Chem. 2000, 72, 1023-1030.
[41] Lu, Q., Collins, G. E., Smith, M., Wang, J., Analytica Chimica Acta 2002, 469, 253-260.
[42] Kohlrausch, F., Ann. Phys. Chem.1897, 62, 209-239.
[43] Tiselius, A., Trans. Faraday Soc. 1937, 33, 524-531.
[44] Hjerten, S., Chromatogr. Rev. 1967, 9, 122-219.
[45] Virtanen, R. Acta Polytechnica Scand. Chem. 1974, 123, 1.
[46] Jorgenson, J. W., Lukacs, K. D., J. Chromatogr. 1981, 218, 209-216.
[47] Jorgenson, J. W., Lukacs, K. D., Anal. Chem. 1981, 53, 1298-1302.
[48] Terabe, S., Otsuka, K., Ichikawa, K., Tsuchiya, A., Ando, T., Anal. Chem. 1984, 56, 111-113.
[49] Hjerten, S., Zhu, M. D., J. Chromatogr. 1985, 346, 265-270.
[50] Hjerten, S., Liao, J. L., Yao, K., J. Chromatogr. 1987, 387, 127-138.
[51] Cohen, A. S., Karger, B. L., J. Chromatogr. 1987, 397, 409-17.
[52] Dittmann, M. M., Rozing, G. P., J. Chromatogr. A 1996, 744, 63-74.
[53] Yan, C., Dadoo, R., Zare, R. N., Rakestraw, D. J., Anex, D. S., Anal. Chem. 1996, 68, 2726-2730.
[54] Hjerten, S., Elenbring, K., Kilar, F., Liao, J., Chen, A. J. C., Siebert, C. J., Zhu, M., J. Chromatogr. 1987, 403, 47-61.
[55] Foret, F., Szoko, E., Karger, B. L., J. Chromatogr. 1992, 608, 3-12.
[56] Schwer, C., Lottspeich, F., J. Chromatogr. 1992, 623, 345-55.
[57] Mazereeuw, M., Tjaden, U. R., Reinhoud, N. J., J. Chromatogr. Sci. 1995, 33, 686-96.
[58] Heiger, D. N., Hewlett-Packard Company Publication Number 12-5091-6199E.
[59] Helmholtz, H. Z., Annal. Phys. Chem. 1879, 7, 337-383.
[60] Heiger, D. N., High Performance Capillary Electrophoresis - An Introduction, 1992, 2nd edition.
[61] Krattiger, B., Bruin, G. J. M., Anal. Chem. 1994, 66, 1-8
[62] Stefansson, M.; Novotny, M., Anal. Chem. 1994, 66, 1134-1140
[63] Kim, Y.; Morris, M.D., Anal. Chem. 1994, 66, 1168-1174
[64] Zhao, Z.; Malik, A.; Lee, M. L., Anal. Chem. 1994, 65, 2747-2752
[65] Chien, R. L., Khaledi, M. G., (Ed.), High Performance Capillary Electrophoresis (Theory, Techniques and Applications), Chapter 13, CRC Press, 1998.
[66] Liu, Z., Sam, P., Sirimanne, S. R., McClure, P. C., Grainger, J., Patterson, D. G., J. Chromatogr. A 1994, 673, 125-132.
[67] Nielson, K. R., Foley, J. P., J. Chromatogr. A 1994, 686, 283-291.
[68] Quirino, J. P., Terabe, S., J. Chromatogr. A 1997, 781, 119-128.
[69] Zhang, C. X., Thormann, W., Anal. Chem. 1998, 70, 540-548.
[70] Shihabi, Z. K., J. Chromatogr. A 1998, 817, 25-30.
[71] Palmer, J.; Munro, N. J.; Landers, J.P., Anal. Chem. 1999, 71, 1679-1687.
[72] Chen, H. L., WHAMPOA - An Interdisciplinary, 2007, 53, 39-46