研究生: |
方晴 Ching Fang |
---|---|
論文名稱: |
毛細管電泳螢光光譜法對尿液中低濃度毒品3,4-亞甲雙氧甲基安非他命(3,4-MDMA)與麥角二乙胺(LSD)之分析研究 Determination of 3,4-Methylenedioxymethamphetamine and Lysergic Acid Diethylamide in Urine by Capillary Electrophoresis/Fluorescence Spectroscopy |
指導教授: |
林震煌
Lin, Cheng-Huang |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2002 |
畢業學年度: | 90 |
語文別: | 中文 |
中文關鍵詞: | 毛細管電泳螢光光譜法 、亞甲雙氧甲基安非他命 、尿液 、麥角二乙胺 |
英文關鍵詞: | LSD |
論文種類: | 學術論文 |
相關次數: | 點閱:868 下載:20 |
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3,4-methylenedioxymethamphetamine(3,4-MDMA)是一種強烈中樞神經興奮劑。本研究發現使用非水相毛細管泳層析/螢光分析法,可提供一種快速、有效的分離條件。利用加入3,4-MDMA-D5為內標物,依照分析物個別的電泳遷移時間與譜峰高度,直接對吸毒者尿液中代謝出的3,4-MDMA濃度做定量分析,每次偵測的時間僅約5分鐘,偵測極限可到1 ppm(5.2 ×10-6 M),輔以簡單的液-液萃取法可使偵測極限降至50 ppb(S/N = 3)。
Lysergic acid diethylamide(LSD)對人體大腦和神經中樞系統具有強大的危害性,受此藥物刺激會產生幻覺及脫離現實感。本研究針對LSD、iso-Lysergic acid diethylamide(iso-LSD)與Lysergic acid N,N-methylpropylamide(LAMPA)建立標準的分析方法。由實驗結果顯示,使用最佳化分離條件為100 mM SDS、50 mM磷酸溶液及3 mM Brij-30並加入Acetonitrile-Methanol-Water(5:35:60, v/v)緩衝溶液組成。此外,本實驗應用毛細管電泳掃集法(sweeping)與陽離子選擇性完全注射掃集MEKC法(CSEI-sweep-MEKC),可使偵測極限(S/N = 3)降至~20 ppb和~60 ppt。最後,本研究以水相毛細管電泳/低溫(77 K)螢光光譜偵測法與GC/MS偵測法應用於檢測嗑藥者尿液與一粒砂毒品中LSD的定性與定量分析。
3,4-methylenedioxyamphetamine (3,4-MDMA) is a strong central nervous system stimulant. Using nonaqueous capillary electrophoresis/fluorescence detection, the precision of the method was evaluated by measuring the repeatability and intermediate precision of migration time and corrected peak height by comparison with a 3,4-MDMA-D5 internal standard. It is possible to determine this drug at a level of 1 ppm without any pre-treatment in less than 5 min. After liquid-liquid extraction, the sample can be condensed and a detection limit of 3,4-MDMA in urine of 50 ppb (S/N = 3) can be achieved.
Lysergic acid diethylamide (LSD) is a powerful psychedelic drug that produces temporary hallucinations and a schizophrenic psychotic state The separation and on-line concentrations of LSD, iso-lysergic acid diethylamide (iso-LSD) and lysergic acid N, N-methylpropylamide (LAMPA) in human urine also were investigated by capillary electrophoresis-fluorescence spectroscopy using a mixture of acetonitrile-methanol-water solution (5:35:60, v/v) containing sodium dodecyl sulfate (100 mM), phosphate (50 mM), and Brij-30 (3 mM). To improve sensitivity, a technique involving sweeping and cation-selective exhaustive injection-sweep-micellar electrokinetic chromato-
graphy (CSEI-sweep-MEKC) were also used for on-line concentration which resulted in detection limits of ~ 20 ppb and ~ 60 ppt, respectively. The separation and on-line identification of LSD in tablets were also investigated by capillary electrophoresis-fluorescence spectroscopy at 77 K and GC/MS.
[1] Ensslin, H.K.; Kovar, K-A.; Maurer, H.H., J. Chromatogr. B 683 (1996) 189
[2] Maurer, H.H.;Bickeboeller-Friedrich, J.; Kraemer, T.; Peters, F.T.,
Toxicol. Letters 112-113 (2000) 133
[3] Sadeghipour, F.; Veuthey, J.-L., J. Chromatogr. A 787 (1997) 137
[4] 管制藥品管理局/公告區/認清MDMA的真面目
http://210.241.68.101/circular/circular_16-1.asp
[5] 管制藥品管理局/預警宣導組/使用快樂丸保證讓你呆
http://210.241.68.101/circular/circular_10.asp
[6] Ortuno, J.; Pizarro, N.; Farre, M.; Mas, M.; Segura, J.; Cami, J.; Brenneisen, R.; Torre, R.de.La., J. Chromatogr. B 723 (1999) 221
[7] Nichols, D.E.; Marona-Lewicka, D.; Huang, X.; Johnson, M.P. Novel Serotonergic Agents. Drug Des. Discovery 1993, 9, 299
[8] Steele, T.D.; McCann, U.D.; Ricaurte,G.A., Addiction 89 (1994) 539
[9] McCann, U.D.; Ricaurte, A.; Shaham, Y.; Ricaurte, G.A., Neuropharmacology.10 (1994) 129
[10] Schneider, S.; Kuffer, P.; Wennig, R., J. Chromatogr. B 713 (1998) 189
[11] M. Nebojsa Djordjevic Fiona Fitzpatrick Fabrice Houdiere, Electrophoresis 21 (2000) 724
[12] 行政院衛生署/管制藥品管理局
http://www.nbcd.gov.tw/prop/prop_1-12.asp
[13] Mcnally, Z.L.;Wang, H.; Salamone, S.J., J. Anal. Toxicol 22 (1998) 520
[14] Poch, G.K.; Klette, K.L.; Anderson, C., J. Anal. Toxicol 24 (2000) 170
[15] Papac, D.I.; Foltz, R.L., J. Anal. Toxicol 14 (1990) 189
[16] Poch, G.K.; Klette, K.L.; Hallare, D.A.; Manglicmot, M.G.;
Czarny, R.J.; McWhorter, L.K.; Anderson, C., J. Chromatogr. B
727 (1999) 23
[17] Siddik, Z.H.; Barnes, R.D.; Dring, L.G.; Smith, R.L.; Williams, R.T., Biochem Pharmacol 28 (1979) 3093
[18] Salamone, S.J.; Li, Z.; McNally, A.J.; Vitone, S.; Wu, R.S., J. Anal. Toxicol 21 (1997) 492
[19] Gan, B.K.; Diane Baugh.; Liu, R.H.; Walia, A.S. J. Forensic Sci 36 (1991) 1331
[20] Rohrich, J.; Zorntlein, S.; Becker, J.J., Forensic Sci. Int 107 (2000) 181
[21] Cody, J.T.; Schwarzhoff, R., J. Anal. Toxicol 17 (1993) 26
[22] Renton, R.J.; Cowie, J.S.; Oon, M. C., Forensic Sci. Int 60 (1993 Aug) 189
[23] Beck, O.; Kraft, M.; Moeller, M.R.; Smith, B.L.; Schneider, S.; Wennig, R., Ann. Clin. Biochem 37 (2000) 199
[24] Sadeghipour, F.; Giroud, C.; Rivier, L.; Veuthey, J.-L., J. Chromatogr. A 716 (1997) 71
[25] Pizarro, N.; Ortuno, J.; Segura, J.; Farre, M.; Mas, M.; Cami, J.; Torre, Rafael de la., Journal of Pharmaceutical and Biomedical Analysis 21 (1999) 739
[26] Ugland, H.G.; Krogh, M.; Rasmuaaen, K.E., Journal of Pharmaceutical and Biomedical Analysis 19 (1999) 463
[27] Kikura, R.; Nakahara, Y.; Mieczkowski, T.; Tagliaro, F., Forensic Sci. Int 84 (1997) 165
[28] Centini, F.; Masti, A.; Comparini, I.B., Forensic Sci. Int 83 (1996) 161
[29] Ramseier, A.; Siethoff, C.; Caslavska, J.; Wolfgang, T., Electrophoresis 21 (2000) 380
[30] Lanz, M.; Brenneisen, R.; Thormann, W., Electrophoresis 18 (1997) 1035
[31] Cox, D.E.; Williams, K.R., Forensic Sci. Int 77 (1996) 101
[32] Steiner, F.; Hassel M, M., Electrophoresis 21 (2000) 3994
[33] Chankvetadze, B.; Blaschke, G., Electrophoresis 21 (2000) 4159
[34] Cherkaoui, S.; Geiser, L.; Veuthey, JL., Chromatographia 52 (2000) 403
[35] Geiser, L.; Cherkaou, S.; Veuthey, JL., J. Chromatogr A 895 (2000) 111
[36] Wang, F.; Khaledi, MG., J. Chromatogr. A 875 (2000) 277
[37] Barron, D.; Irles, A.; Barbosa, J., J. Chromatogr. A 871 (2000) 367
[38] Lin, CH.; Chung, YL.; Chen, YH., Analyst 126 (2001) 302
[39] Chung, YL.; Liu, JT.; Lin, CH., J. Chromatogr. B 759 (2001)
219
[40] Paul, B.D.; Smith, M.L., Forensic Sci. Rev 11 (1999) 158-174
[41] Hollister, L.E.; Thomas, S.R. (Ed.), Hallucinogens (1974) 173
[42] Smith, R.N.; Robinson, K., Forensic Sci. Int 28 (1985) 229
[43] Taunton-Rigby, A.; Sher, S.E., Kelley, P.R., Science 181 (1973) 165
[44] Christie, J., White, M.W., Wiles, J.M., J. Chromatogr 120 (1976) 496
[45] McCarron, M.M., Walberg, C.B., Baselt, R.C., J. Anal. Toxicol 14 (1990) 165
[46] Marquet, P.; Lachatre, G., J. Chromatogr. B 773 (1999) 93
[47] Hoja, H.; Marquet, P.; Verneuil, B.; Lotfi, H.; Dupuy, J.-L.; Lachatre, G., J. Chromatogr. B 692 (1997) 329
[48] Lim, H.K., Andrenyak, D., Francom, P., Foltz, R.L., Jones, R.T., Anal. Chem 60 (1988) 1420
[49] Papac, D.I., Foltz, R.L., J. Anal. Toxicol 14 (1990) 189
[50] Paul, B.D.; Mitchell, J.M.; Burbage, R.; Moy, M.; Sroka, R., J. Chromatogr 529 (1990) 103
[51] Nelsom, C.C., Foltz, R.L., Anal. Chem 64 (1992) 1578
[52] Todorowic, R.; Ariese, F.; Devanesan, P.; Jankowiak, R.; Small, G.J.; Rogan, E.G.; Cavalieri, E.L., Chem. Res. Toxicol 10 (1997) 941
[53] Zamzow, D.; Lin, C.-H.; Small,G.J.; Jankowiak, R., J. Chromatogr. A 781 (1997) 73
[54] Ariese, M.F.; Small, G.J.; Jankowiak, R.; Hewer, A.; Phillips, D. H., Carcinogenesi 16 (1995) 2561
[55] Jankowiak, R.; Zamzow, D.; Ding, W.; Small, G.J., Anal. Chem 68 (1996) 2549
[56] Roberts, K.P.; Lin, C.-H.; Jankowiak, R.; Small, G.J., J. Chromatogr. A 853 (1999) 159
[57] Jankowiak, R.; Roberts, K.P.; Small, G.J., Electrophoresis 21 (2000) 1251
[58] Roberts, K.P.; Lin, C.-H.; Singhal, M.; Casale, G.P.; Small, G.J.; Jankowiak, R., Electrophoresis 21 (2000) 799
[59] Jankowiak, R.; Lin, C.-H.; Zamzow, D.; Roberts, K.P.; Lim, K.-M.; Small, G.J., Chem. Res. Toxicol 12 (1999) 768
[60] Quirino, J.P.; Terabe, S., Science 282 (1998) 465
[61] Quirino, J.P.; Terabe, S., Anal. Chem 72 (2000) 1023
[62] White, S.A.; Catterick, T.; Harrison, M.E.; Johnston, D.E.; Reed, G.D.; Webb, K.S., J. Chromatogr. B 692 (1997) 335
[63] Varesio, E.; Veuthey, J-L., J. Chromatogr. A 717 (1995) 219
[64] Kohlrausch, F.; Wiedemanns., Ann. Phys. Chem 62 (1897) 209
[65] Tiselus, A., Trans. Faraday. Soc 33 (1937) 524
[66] Knox, J. Chromatogr 26 (1988) 329
[67] Hjerten, S., Chromatogr. Rev 9 (1967) 122
[68] Virtanen, R., Acta Polytechnica Scand 123 (1979) 1
[69] Joegenson, J.W.; Lukacs, K.D., J. Chromatogr 218 (1981) 209
[70] Joegenson, J.W.; Lukacs, K.D., Anal. Chem 53 (1981) 1298
[71] Otsuka, S.K.; Ichikawa, K.; Tsuchiya, A.; Ando, T., Anal. Chem 56 (1984) 111
[72] Row, K.H.; Griest, W.H.; Maskarienc, M.P., J. Chromatogr 409 (1987) 193
[73] Hjerten, S.; Zhu, M.D., J. Chromatogr 346 (1985) 265
[74] Cohen , A.; Karger, B.L., J. Chromatogr 397 (1987) 409
[75] Huang, X.; Zare, R.N., Anal. Chem 63 (1991) 2193
[76] Huang, X.; Gordon, M.J.; Zare, R.N., Anal. Chem 60 (1988) 375
[77] Kennedy, R.T.; Jorgenson, J.W., Anal. Chem 61 (1989) 1128
[78] Yan, C.Y.; Dadoo, R.; Zare, R.N.; Rakestraw, D.J.; Anex, D.S., Anal. Chem 68 (1996) 2726
[79] Foret, F.; Krivankova, L.; Bocek, P. Capillary Zone Electrophoresis P8VCH, Weiheim, 1993.
[80] Helmholtz, H. Z. Anal., Phys. Chem 7 (1897) 337
[81] David N. Heiger High Performance Capillary Electrophoresis - An Introduction, 1992, 2nd edition.
[82] Jandik, P.; Bonn, G. Capillary Electrophoresis of Small Molecules and Ions, VCH, New York, 1993.
[83] Krattiger, B.; Bruin, G.J.M.; Bruin, A.E., Anal. Chem 66 (1994) 1
[84] Stefansson, M.; Novotny, M., Anal. Chem 66 (1994) 1134
[85] Kim, Y.; Morris, M.D., Anal. Chem 66 (1994) 1168
[86] Zhao, Z.; Malik, A.; Lee, M. L., Anal. Chem 65 (1994) 2747
[87] Shpol’skii, E.V.; Il’ina, A.A.; Klimova, L.A.; Dokl. Akad. Nauk SSSR 87, 935 (1952).
[88] Albin, M.; Grossman, P.D.; Moring, S.E., Anal. Chem 65 (1993) 489
[89] Quirino, J.P.; Terabe, S., J. High Resoln Chromatogr 22 (1999) 367
[90] Quirino, J.P.; Terabe, S.; Otsuka, K.; Vincent, J.B.; Vigh, G., J. Chromatogr. A 838 (1999) 3
[91] Quirino, J.P.; Terabe, S., J. Chromatogr. A 902 (2000) 119
[92] R.L. Chien, High performance Capillary Electrophoresis (Theory,
Techniques and Applications), Chapter 13, M.G. Khaledi, Ed., CRC Press, 1998.
[93] Liu, Z.; Sam, P.; Sirimanne, S.R.; McClure, P.C.; Grainger, J.; patterson, D.G., J. Chromatogr. A 673 (1994) 125
[94] Nielson, K.R.; Foley, J.P., J. Chromatogr. A 686 (1994) 283
[95] Quirino, J.P.; Terabe, S., J. Chromatogr. A 781 (1997) 119
[96] Zhang, C.X.; Thormann, W., Anal. Chem 70 (1998) 540
[97] Shihabi, Z.K., J. Chromatogr. A 817 (1998) 25
[98] Palmer, J.; Munro, N.J.; Landers, J.P., Anal. Chem 71 (1999) 1679
[99] Taylor, K.Z.; Waddell, D.S.; Reiner, E.J.;Macpherson,K.A., Anal. Chem 67 (1995) 1186
[100] Chiron, S.; Dupas, S.; Scribe, P.; Barcelo, D., J. Chromatogr. A 665 (1994) 295
[101] Barcelo, D., J. Chromatogr 643 (1993) 117
[102] Butz, S.; Heberer, Th.; Stan, H.-J., J. Chromatogr. A 677 (1994) 63
[103] Huang, W.; Andollo, W.; Hearn, W. L., J. Anal. Toxicol 16 (1992) 307
[104] Lee, M.-R.; Yu, S.-C.; Lin, C.-L.; Yeh, Y.-C., J. Anal. Toxicol 21 (1997) 278
[105] 鍾裕霖,非水相毛細管電泳/低溫螢光分析法對尿液中3,4-亞甲雙氧甲基安非他命(3,4-MDMA)之分析研究,國立台灣師範大學,化學研究所,碩士論文,台北,2001.