研究生: |
方晴 Ching Fang |
---|---|
論文名稱: |
毛細管電泳螢光光譜法對尿液中低濃度毒品3,4-亞甲雙氧甲基安非他命(3,4-MDMA)與麥角二乙胺(LSD)之分析研究 Determination of 3,4-Methylenedioxymethamphetamine and Lysergic Acid Diethylamide in Urine by Capillary Electrophoresis/Fluorescence Spectroscopy |
指導教授: |
林震煌
Lin, Cheng-Huang |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2002 |
畢業學年度: | 90 |
語文別: | 中文 |
中文關鍵詞: | 毛細管電泳螢光光譜法 、亞甲雙氧甲基安非他命 、尿液 、麥角二乙胺 |
英文關鍵詞: | LSD |
論文種類: | 學術論文 |
相關次數: | 點閱:893 下載:20 |
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3,4-methylenedioxymethamphetamine(3,4-MDMA)是一種強烈中樞神經興奮劑。本研究發現使用非水相毛細管泳層析/螢光分析法,可提供一種快速、有效的分離條件。利用加入3,4-MDMA-D5為內標物,依照分析物個別的電泳遷移時間與譜峰高度,直接對吸毒者尿液中代謝出的3,4-MDMA濃度做定量分析,每次偵測的時間僅約5分鐘,偵測極限可到1 ppm(5.2 ×10-6 M),輔以簡單的液-液萃取法可使偵測極限降至50 ppb(S/N = 3)。
Lysergic acid diethylamide(LSD)對人體大腦和神經中樞系統具有強大的危害性,受此藥物刺激會產生幻覺及脫離現實感。本研究針對LSD、iso-Lysergic acid diethylamide(iso-LSD)與Lysergic acid N,N-methylpropylamide(LAMPA)建立標準的分析方法。由實驗結果顯示,使用最佳化分離條件為100 mM SDS、50 mM磷酸溶液及3 mM Brij-30並加入Acetonitrile-Methanol-Water(5:35:60, v/v)緩衝溶液組成。此外,本實驗應用毛細管電泳掃集法(sweeping)與陽離子選擇性完全注射掃集MEKC法(CSEI-sweep-MEKC),可使偵測極限(S/N = 3)降至~20 ppb和~60 ppt。最後,本研究以水相毛細管電泳/低溫(77 K)螢光光譜偵測法與GC/MS偵測法應用於檢測嗑藥者尿液與一粒砂毒品中LSD的定性與定量分析。
3,4-methylenedioxyamphetamine (3,4-MDMA) is a strong central nervous system stimulant. Using nonaqueous capillary electrophoresis/fluorescence detection, the precision of the method was evaluated by measuring the repeatability and intermediate precision of migration time and corrected peak height by comparison with a 3,4-MDMA-D5 internal standard. It is possible to determine this drug at a level of 1 ppm without any pre-treatment in less than 5 min. After liquid-liquid extraction, the sample can be condensed and a detection limit of 3,4-MDMA in urine of 50 ppb (S/N = 3) can be achieved.
Lysergic acid diethylamide (LSD) is a powerful psychedelic drug that produces temporary hallucinations and a schizophrenic psychotic state The separation and on-line concentrations of LSD, iso-lysergic acid diethylamide (iso-LSD) and lysergic acid N, N-methylpropylamide (LAMPA) in human urine also were investigated by capillary electrophoresis-fluorescence spectroscopy using a mixture of acetonitrile-methanol-water solution (5:35:60, v/v) containing sodium dodecyl sulfate (100 mM), phosphate (50 mM), and Brij-30 (3 mM). To improve sensitivity, a technique involving sweeping and cation-selective exhaustive injection-sweep-micellar electrokinetic chromato-
graphy (CSEI-sweep-MEKC) were also used for on-line concentration which resulted in detection limits of ~ 20 ppb and ~ 60 ppt, respectively. The separation and on-line identification of LSD in tablets were also investigated by capillary electrophoresis-fluorescence spectroscopy at 77 K and GC/MS.
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