研究生: |
陳美蘭 Mei-Lan Chen |
---|---|
論文名稱: |
台灣中海拔地區芒草之遺傳結構研究 |
指導教授: |
黃生
Huang, Shong |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
論文出版年: | 2001 |
畢業學年度: | 89 |
語文別: | 中文 |
論文頁數: | 77 |
中文關鍵詞: | 台灣芒 、族群遺傳結構 、基因流傳 、遺傳分化 |
英文關鍵詞: | Miscanthus sinensis var. formosanus Hack, genetic strusture, gene flow, genetic differentiation |
論文種類: | 學術論文 |
相關次數: | 點閱:797 下載:11 |
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本研究利用同功酶方法檢測台灣中海拔地區台灣芒(Miscanthus sinensis var. formosanus Hack)的(1)天然族群遺傳結構;(2)種子穗的遺傳變異,以及(3)台灣芒與白背芒及高山芒的分化程度。結果發現成株和幼苗葉組織同功酶表現型不同,成體組織可判讀9種酶,16個基因座;幼苗組織則可判讀6種酶,6個基因座,分析數據獲得以下結果:(1)台灣芒六個族群的異合度期望值(HE)平均為0.202,族群間的遺傳分化百分比(GST)平均值為0.09,與一般的風媒物種相近。(2)台灣芒變種內的族群分化指數(FST=0.093),大於變種間的指數(FST=0.056),顯示台灣芒與兩變種族群間有充分的基因流傳。(3)台灣芒行異配有性生殖(tm=0.931),花粉在族群內的流傳相當順暢,種子穗的父本花粉可能等位基因頻度,和原母族群高度相似(0.998以上)。
Miscanthus sinensis var. formosanus Hack is a grass species growing in 1200-2000 M elevation mountain areas in Taiwan, where the species grows is between the distribution of M. sincesis var. glaber (Naki) J. Lee and M. sincesis var. transmorrisonensis Hayata.
In this study, isozyme electrophoresis was applied to detect the (1) genetic strusture of natural populations; (2) genetic variation of spike's offspring and (3) the genetic differentiation among these three varieties in species of Miscanthus sinensis in Taiwan. 16 loci from nine stains was used to analyze the isozymic variation of adult individuals, 6 loci from six stains for spike offspring. The results shown the average of expected heterozygosity (HE) is 0.202 and the coefficient of gene differentiation (GST) is 0.09, both demonstrated the similar parameter to mix-mating wind-pollinated species. The FST values from 6 populations of M. sinensis var. formosanus Hack are 0.093, which is higher than the genetic differentiation (FST=0.056) among the populations of the three varieties in Nantou. The higher FST is showing the gene flow among these three varieties is greater than other different populations of M. sinensis var. formosanus Hack. The estimation of multilocus outcrossing rate, tm=0.89, suggests an outcrossing mating strategy in M. sinensis var. formosanus Hack. In addition, the study of genetic variation from spike offspring indicated that genetic composition from a single spike is indistinguishable from its natural population, the genetic similarity is as high as 0.998. The genetic homogeneity implies a high gene flow within M. sinensis var. formosanus Hack population.
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