研究生: |
林鳳群 |
---|---|
論文名稱: |
八通道高溫超導免疫分析儀研製及Tau蛋白檢測應用 The development of 8-channel high-Tc Superconducting-Quantum-Interference-Device-based immunoassay analyzer with Tau protein detection |
指導教授: |
洪姮娥
Horng, Herng-Er 謝振傑 Chieh, Jen-Jie |
學位類別: |
碩士 Master |
系所名稱: |
光電工程研究所 Graduate Institute of Electro-Optical Engineering |
論文出版年: | 2014 |
畢業學年度: | 102 |
語文別: | 中文 |
論文頁數: | 46 |
中文關鍵詞: | 超導量子干涉元件 、磁減量 |
論文種類: | 學術論文 |
相關次數: | 點閱:184 下載:4 |
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近年來超導量子干涉元件(Superconducting Quantum Interference Device, SQUID)頻繁的應用在生物醫學上。本研究使用了高溫超導量子干涉元件,研製八通道高溫超導免疫分析儀,並配合實驗室研發的生物分子標記磁性奈米粒子和超靈敏磁減量(Immuno Magnetic Reduction,IMR)免疫檢測技術。以往高溫超導免疫分析儀在檢測磁性標記的免疫測定法時,常常因為通道數不夠,單一樣品檢測就非常耗時,所以我們為提升四通道高溫超導免疫分析儀,所以進行的技術上的改進,並配合檢測Tau 蛋白磁減量,來確認八通道高溫超導免疫分析儀的可靠性。
八通道高溫超導免疫分析儀為了符合量測速率的需求,提高檢測通道,使日益增長的檢測量需求暫且得到滿足。在實驗過程中,也藉著增進四通道的效能,改變了一些器件,在Switch中我們改變了電路板的電路圖及繼電器元件,增加了量測上的彈性;也改變了線圈組的長度,將原本四通道線圈組的長度縮短二分之一,在日後如需要量產製作的時候,能夠將時間縮短很多,且空間方面也占了比較小的空間;接線方面,也將BNC接頭換成屏蔽效果較佳的鍍金SMA接頭。目前八通道高溫超導免疫分析儀成了如要量測多種樣品時最好的選擇,只需要簡易的方法配合上試劑即可得到結果,非常符合生物醫學快速簡便的要求。
1. 楊鴻昌,〈最敏感的感測元件SQUID 及其前瞻性應用〉,《物理雙月刊》,第24 卷,第5期,第652–665 頁,2002 年10 月。
2. 楊鴻昌,〈最敏感的感測元件SQUID 及其前瞻性應用〉,《物理雙月刊》,第24 卷,第5期,第652–665 頁,2002 年10 月。
3. 楊鴻昌,〈認識超導量子干涉元件(SQUID)〉,《物理雙月刊》,第11 卷,第5 期,第454–457頁,1989 年10 月。
4. A. H. Silver and J. E. Zimmerman, “Quantum transitions and loss in multiply connected superconductors,” Phys. Rev. Lett., vol. 15, pp. 888–891, Dec. 1965.
5. Che-Chuan Yang, Shieh-Yueh Yang,,Jen-Jie Chieh, Herng-Er Horng, Chin-Yih Hong,Hong-Chang Yang, K. H. Chen, B. Y. Shih, Ta-Fu Chen, and Ming-Jang Chiu,"Biofunctionalized Magnetic Nanoparticles for Specifically Detecting Biomarkers of Alzheimer’s Disease in Vitro,"ACS Chem. Neurosci. 2011, 2, 500–505.
6. J. J. Chieh, S. Y. Yang, Z. F. Jian, W. C. Wang, and H. E. Horng,"Hyper-high-sensitivity wash-free magneto reduction assay on biomolecules using high-Tc superconducting quantum interference devices",JOURNAL OF APPLIED PHYSICS 103, 014703 2008 .
7. 磁性奈米粒子在體外的應用,楊謝樂。
8. 磁性奈米粒子於生物醫學上之應用,楊謝樂。
9. Ming-Jang Chiu,Ya-Fang Chen,Ta-Fu Chen, Shieh-Yueh Yang,Fan-Pei Gloria Yang, Tien-Wen Tseng, Jen-Jie Chieh, Jia-Chun,Rare Chen,Kai-Yuan Tzen,Mau-Sun Hua,and Herng-Er Horng,"Plasma Tau as a Window to the Brain—Negative Associations With Brain Volume and Memory Function in Mild Cognitive Impairment and Early Alzheimer’s Disease”, Human Brain Mapping 00:00–00 (2013).
10. M. J. Chiu, H. E. Horng, J. J. Chieh, S. H. Liao, C. H. Chen, B. Y. Shih, C. C. Yang, C. L. Lee, T. F. Chen,S. Y. Yang, C. Y. Hong, and H. C. Yang,"Multi-Channel SQUID-Based Ultra-High-Sensitivity In-Vitro Detections for Bio-Markers of Alzheimer’s Disease Via Immunomagnetic Reduction,"IEEE, VOL. 21, NO. 3, JUNE 2011.
11. S.Y. Yang, J.J. Chieh,W.C.Wang, C.Y. Yu, C.B. Lana, J.H. Chen, H.E. Horng,,Chin-Yi Hong, H.C. Yang,Wilber Huang,"Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays”, Journal of Virological Methods 153 (2008) 250–252
12. J. J. Chieh, Shieh-Yueh Yang, Herng-Er Horng, C. Y. Yu, C. L. Lee et al.,"Immunomagnetic reduction assay using high-Tc superconducting quantum-interference-device-based magnetosusceptometry",JOURNAL OF APPLIED PHYSICS 107, 074903 2010 .
13. C. C. Yang, K. W. Huang, S. Y. Yang, H. H. Chen, T. C. Chen, C. S. Ho, S. F. Chang,J. J. Chieh, H. E. Horng,C. Y. Hong, and H. C. Yang,"Development for High-Accuracy In Vitro Assay of Vascular Endothelial Growth Factor Using Nanomagnetically Labeled Immunoassay",Journal of Nanomaterials Volume 2013, Article ID 695276, 7 pages.
14. Chin.Yih.Hong,Hong,C.C.Wu,Y.C.Chiu,S.Y.Yang,H.E.Horn,andH.C.Yang,"Magnetic susceptibility reduction method for magnetically labeled immunoassay",Appl. Phys. Lett.,88,212512(2006).
15. C.Y.Hong,W.H.Chen,C.F.Chien,S.Y.Yang,H.E.Horng,L.C.Horng,L.C.Yang,and H.C.Yang,Appl.phys.Lett.,90,74105,(2007).
16. 物理雙月刊(二十八卷四期)2006年8月 697。
17. H.W.Davies,and J.Patrick Llewllyn,J.Phys.D.,12,1357,(1979).
18. S.Y.Yang,J.S.Sun,C.H.Liu,Y.H.Tsuang,L.T.Chen,C.Y.Hong,H.C.Yang,and H.E.Horng,Artificial Organs,32,195,(2008).
19. H.E.Horng,S.Y.Yang,C.M.Liu,P.-S.Tsai,C.Y.Hong,H.C.Yang,and C.C.Wu,Appl.Phys.Lett.,88,252506,(2006).
20. “User Manual for JSQ rf SQUID Magnetomerer and rf SQUID Electronic with Touch Panel Controller 1 chennel SQUID System”,JSQ.