破囊壺菌是非光合性的海產原生生物，屬於羽狀鞭毛菌群。它對於鈉有絕對的需求性，因此廣泛分佈於海洋、河口、內陸性鹽水湖泊及紅樹林等地域。菌體為單中心，外形和單中心體的壺菌相似，惟其游孢子具有雙鞭毛，或為不具鞭毛的不動孢子。在生活史方面，無性生殖包括孢子囊與外質網，大部分為在藻類及顯花植物殘屑上營腐生，另在無脊椎動物身上營寄生亦有數例，有性生殖至今尚未被發現。
從台灣北部沿海地區採得水樣及土樣，以台灣二葉松花粉作為餌物進行破囊壺菌的誘釣，並使用含抗生素的KMV培養基，以塗佈及劃線的方式分離與純化破囊壺菌。獲得純培養後，將破囊壺菌轉移至不含抗生素之KMV培養基上或以無菌水加入滅菌過的花粉誘釣，作為菌種的觀察及形態學上描述和鑑定之依據。
實驗結果共獲得三屬九種，包括破囊壺菌屬（Thraustochytrium）五種：即是動孢破囊壺菌（T. motivum Goldstein）、南極破囊壺菌（T. antarcticum Bahnweg and Sparrow）、增生破囊壺菌（T. proliferum Sparrow）、金尼破囊壺菌（T. kinnei Gaertner）和聚合破囊壺菌（T. aggregatum Ulken）；吾肯氏壺菌屬（Ulkenia）二種：即是威瑟吾肯氏壺菌(U. visurgensis (Ulken) Gaertner)和星狀吾肯氏壺菌(U. radiata Gaertner)以及裂殖壺菌屬(Schizochytrium)二種：即是八孢裂殖壺菌(S. octosporum Kumer)和紅樹林裂殖壺菌(S. mangrovei Kumer)。其中，吾肯氏壺菌屬（Ulkenia）為台灣新紀錄屬；另新紀錄種有六種，分別為：南極破囊壺菌（T. antarctium）、金尼破囊壺菌（T. kinnei Gaertner）、威瑟吾肯氏壺菌(U. visurgensis)、星狀吾肯氏壺菌(U. radiata)、八孢裂殖壺菌(S. octosporum)和紅樹林裂殖壺菌(S. mangrovei)。

Abstract
Thraustochytrids are non- photosynthetic marine protests, belonging to chromists. They display an obligate sodium requirement, so they are widely distributing in the ocean, estuary, inland salt lakes and the mangroves. The thalli of thraustrids are similar to the monocentric chytrids. Thraustochytrids have biflagellate zoospores and non-flagellate aplanospores. They are saprophytic on algae and phanerogamic detritus and parasitic on invertebrate. In the asexual reproduction, the thallus includes the sporangium and ectoplasmic net. So far, no sexual reproduction have been documented in any thraustochytrids.
Thraustochytrids are isolated from the soil and water samples collected in the north coast of Taiwain by using pine pollen grains(Pinus taiwainensis). All isolates were successively transferred by streaking on the KMV agar medium containing antibiotics until the cultures were purified. Then transferred the thraustochytrids on the KMV agar medium and in pollen seawater. In such culture conditions, observation and discription of their morphological characteristics were made.
Totally, three genera and nine species are successfully examined and identified namely : five species of Thraustochytrium : T. motivum 、T. antarcticum 、T. proliferum 、T. kinnei 、T. aggregatum；two species of Ulkenia : U. visurgensis 、 U. radiata ；two species of Schizochytrium ：S. octosporum S. mangrovei. Among them, T. antarctium、T. kinnei 、U. visurgensis、U. radiata、S. octosporum、S. mangrove are described as new records to Taiwain.

Reference:
Alexopoulos, C. J. , C. W. Mims , and M. Blackwell. 1996. Phylum Labyrinthulomycota net slime molds. Pp.743-750. In Introductory Mycology.
Amon, J. P. 1978. Thraustochytrids and Labyrinthulids of terrestrial, aquatic and hypersaline environments of the great salt lake, USA. Mycologia 70:1299-1301.
Bahnweg, G. and F. K. Sparrow, Jr. 1974. Four new species of Thraustochytrium from antarctic regions, with notes on the distribution of zoosporic fungi in the antarctic marine ecosystems. Amer. J. Bot. 61(7):754-766.
Barclay, W. 1996. Nutritional enhancement of n-3 and n-6 fatty acids in rotifers and Artemia Nauplii by feeding spradried schizochytrium sp.Journal of the world aquaculture society 27(3):314-322.
Bremer, G. B. 1995. Lower marine fungi (labyrinthulomycetes) and the decay of mangrove leaf litter. Hydrobiologia 295:89-95.
Booth, T. and C. E. Miller. 1968. Comparative morphologic and taxonomic studies in the genus thraustochytrium. Mycologia 60:480-495.
Bower, S. M., D. J. Whitaker, and D. Voltolina. 1989. Resistance to ozone of zoospores of the thraustochytrid Abalone parasite, Labyrinthuloides haliotidis.(Protozoa: Labyrinthomorpha). Aquaculture. 78:147-152.
Caron, D. A. 1987. Grazing of attrched bacteria by heterotrophic microflagellates. Microb. Ecol. 13:203-218.
Cavalier-smith, T., M. T. E. P. Allsopp, and E. E. Chao. 1994.Thraustochytrids are chromists, not fungi:18s rRNA signatures of heterokonta. Phil. Trans. R. Soc. Lond. B 346:387-397.
Coleman, N. K. and J. R. Vestal. 1987. An epifluorescent microscopy study of enzymatic hydrolysis of fluorescein diacetate associated with the ectoplasmic net elements of the protist Thraustochytrium striatum. Can. J. Microbiol. 33:841-843.
Darley, W. M., D. Porter, And M. S. Fuller. 1973. Cell wall composition and synthesis via golgi-directed scale formation in the marine Eucaryote , Schizochytrium aggregatum, with a note on Thraustochytrium sp. Arch. Mikrobiol. 90:89-106.
Dykstra, M. J. 1984. Diplophrys marina, a new scale-forming marine protist with Labyrinthulid affinities. Mycologia 76(4):626-632.
Fuller, M. S., B. E. Fowles, and D. J. McLaughlin. 1964. Isolation and pure culture study of marine Phycomycetes. Mycologia 56:745-756.
Gaertner, A. 1968. Eine methode des quantitativen nachweises nierderer, mit pollen koederbarer pilze im meerwasser und im sediment. Veroff. Inst. Meeresforsch. Bremerh. 3:75-92.
Gaertner, A. 1969. Marine niedere pilze in nordsee und nordatlantik. Ber. Dtsch. Bot. Ges. Bd.82:287-306.
Gaertner, A. 1970. Beobachtungen uber die sporulation der dickwandigen sporangien von Thraustochytrium kinnei Gaertner. Veroff. Inst. Meeresforsch. Bremerh. 12:321-327.
Gaertner, A. 1971. Thraustochytrium kinnei (thraustochytriaceous) Vegetative entwicklung. Editor by G. Wolf. Inst. Fur den Wwissenscha. Film. (B1078/1971), Gottingen.
Gaertner, A. 1972. Characters used in the classification of thraustochytriaceous fungi. Veroff. Inst. Meeresforsch. Bremerh.13:183-194.
Gaertner, A. 1972. Sporulation bei thraustochytriaceae. Inst. Fur den Wissenscha. Film. (B1078/1971), Gottingen.
Gaertner, A. 1977. Revision of the thraustochytriaceae (lower marine fungi) I. Ulkenia nov. gen., with description of three new species. Veroff. Inst. Meeresforsch. Bremerh. 16:139-157.
Gaertner, A. 1981. A new marine phycomycete, Schizochytrium minutum, sp. nov. ( thraustochytriaceae ) from saline habitats. Veroff. Inst. Meeresforsch. Bremerh. 19:61-69.
Goldstein, S. 1963a. Morphological variation and nutrition of a new monocentric marine fungus. Arch. Mikrobiol. 45:101-110.
Goldstein, S. 1963b. Studies of a new species of thraustochyytrium that displays light stimulated growth. Mycologia 55:799-811.
Goldstein, S. 1963b. Development and nutrition of new species of thraustochytrium. Am. J. Bot. 50:271-279.
Goldstein, S. 1973. Zoosporic marine fungi (thraustochytriaceae and dermocystidiaceae). Ann. Rev. Microbiol. 27:13-26.
Honda, D., T. Yokochi, T. Nakahara, M. Erata, and T. Higashihara. 1998. Schizochytrium limacinum sp. nov., a new thraustochytrid from a mangrove area in the west Pacific Ocean. Mycol. Res. 102(4):439-448.
Iida, I., T. Nakahara, T. Yokochi, Y. Kamisaka, H. Yagi, M. Yamaoka, and O. Suzuki. 1995. Improvement of docosahexaenoic acid production in a culture of thraustochytrium aureum by medium optimization. J. Ferment. Bioeng. 81(1)76-78.
Karling, J. S. 1979. The anisochytrid genus Elina. Mycologia 71:829-830.
Karling, J. S. 1981. Predominantly holocarpic and eucarpic simple biflagellate phycomycetes. 2nd ed. pp165-198. 13pls. J. Cramer. Germany.
Kazama, F. Y., A. L. Zachary, and K. L. Schornstein. 1975. Observations on Thraustochytrim sp.:development and behavior in culture.Can.J.Bot. 53:360-374.
Kendrick, A., and C. Ratledge. 1992. Lipids of selected Molds grown for production of n-3 and n-6 polyunsaturated fatty acids. Lipids 27(1):15-20.
Kumar, S. R. 1981. Fine structure of the Thraustochytrid Ulkenia amoeboidea. I. Vegetative thallus and formation of the amoeboid stage. Can. J. Bot. 60:1092-1102.
Kumar, S. R. 1982. Fine structure of the Thraustochytrid Ulkenia amoeboidea. II. The amoeboid stage and formation of zoospores. Can. J. Bot. 60:1103-1114.
Kumar, S. R. 1987. Occurrence of the Thraustochytrid, Corallochytrium limacisporum gen. et sp. nov. in the coral reef lagoons of the lakshadweep islands in the Arabian Sea. Botanica Marina 30:83-89.
Kumar, S. R.. 1988a. Detection of the thraustochytrid protist Ulkenia visurgensis in a hydroid, using immunofluorescence. Marine Biol. 97:253-258.
Kumar, S. R. 1988b. Schizochytrium octosporum sp. Nov. and other Thraustochytrids from the north sea.(Rosfjord,Norway). Trans. Br. Mycol. Soc. 90(2):273-278.
Kumar, S. R. 1988c. Schizochytrium mangrovei sp. Nov., a Thraustochytrid from Mangroves in India. Trans. Br. Mycol. Soc. 90(4):627-631.
Kumar, S. R. 1992. Bacterivory: a novel dual role for thraustochytrids in the sea. Marine Biol. 113:165-169.
Kumar, S. R. 1996. Morphology, taxonomy and ecology of thraustochytrids and labyrinthulids, the marine counterparts of zoosporic fungi. Pp. 35-60. In:Advances in zoosporic fungi. Eds. R. Dayal. MD publ. Put, Ltd, New Delhi.
Kumar, S. R., S. Nagarkar, and S. Raghukumar. 1992. Association of Thraustochytriums and fungi with living marine algae.Mycol. Res. 96(7):542-546.
Kumar, S. R., S. Sharma, C. Raghukumar, V. Sathe-Pathak, and D. Chandramohan. 1994. Thraustochytrid and fungal component of marine detritus. IV. Laboratory studies on decomposition of leaves of the mangrove Rhizophora apiculata Blume. J. Exp. Mar. Biol. Ecol. 183:113-131.
McLean, N, and D. Porter. 1982. The yellow-spot disease of Tritonia Diomedea Bergh, 1984 (Mollusca : Gastropoda : Nudibranchia) : encarpsulation of the thraustochytriaceous parasite by host amoebocytes. J. parasitol. 68(2):243-252.
Moss, S. T. 1980. Ultrastructure of the endomembrane-sagenogenetosome-ectoplasmic net complex in Ulkenia visurgensis(Thraustochytriales). Bot. Mar. 23:73-94.
Olive, L. S. 1975. Labyrinthulina (Labyrinthulas and Thraustochytrids). Pp.215-241. In :The Mycetozoans. Academic Press, N. Y.
Perkins, F. O. 1972. The ultrastructure of holdfasts,“rhizoids”, and “Slime tracks”in thraustochytriaceous Fungi and Labyrinthula spp. Arch. Mikrobiol. 84:95-118.
Polglase, J. L. 1980. A Preliminary report on the thraustochytrid(s) and Labyinthulid(s) associated with a pathological condition in the lesser octopus Eledone cirrhosa. Bot. Mar. 23:699-706.
Porter, D. and G. Kochert. 1978. Ribosomal RNA Molecular weights and the phylogeny of Labyrinthula. Experimental Mycology. 2:346-351.
Porter, D. and W. L. Lingle. 1992. Endolithic thraustochytrid marine fungi from planted shell fragments. Mycologia 84(3):289-299.
Porter, D. 1984. Diplophrys marina, a new scale-forming marine protist with labyrinthulid affinities. Mycologia 76(4):626-632.
Porter, D. 1987a. Labyrinthulomycetes. Pp110-111. In M. Fuller , and A. Jaworski (eds.) , Zoosporic fungi in teaching and research. Southeastern Publ. Coorp. , Athens, Georgia, U.S.A.
Porter, D. 1987b. Thraustochytriaceae. Pp110-111. In M. Fuller , and A. Jaworski (eds.) , Zoosporic fungi in teaching and research. Southeastern Publ. Coorp. , Athens, Georgia, U.S.A.
Porter, D. 1987c. Isolation of zoosporic marine fungi. Pp128-129. In M. Fuller , and A. Jaworski (eds.) , Zoosporic fungi in teaching and research. Southeastern Publ. Coorp. , Athens, Georgia, U.S.A.
Porter, D. 1989. Phylum Labyrintholomycota. Pp. 388-398. In: Handbook of Protoctista. Eds. L. Margulis, J. O. Corliss, M. Melkonian and D. J. Chapman. Jones and Bartlett , Boston, MA.
Raghukumar, C., S. Nagarkar, and R. S. kumar. 1992. Association of thraustochytrids and fungi with living marine algae. Mycol. Res. 96(7):542-546.
Ross, I. N.1979. The Gymnomyxa: the slime molds and associates. Pp210-215. In Biology of The Fungi.
Sathe, V. and S. R. kumar. 1991. Fungi and their biomass in detritus of the seagrass Thalassia hemprichii (Ehrenberg) ascherson. Bot. Mar. 34:271-277.
Schneider, V. J. 1967. Ein neuer mariner phycomycet aus der kieler bucht (Thraustochytrium striatum spec. nov.). Kieler Meeresforsch, 23:16-20.
Sharma, S., C. Raghukumar,S. R. kumar, V. Sathe-Pathak, and D. Chandramohan. 1994. Thraustochytrid and fungal component of marine detritus II. Laboratory studies on decomposition of the brown alga Sargassum cinereum J. Ag. J. Exp. Mar. Biol. Ecol. 175:227-242.
Singh, A., S. Wilson, and O. P. Ward.1996. Docosahexaenoic acid (DHA) production by thraustochytrium sp. ATCC 20892. World journal of Microbiology & Biotechnology. 12:76-81.
Singh, A., and O. P. Ward.1996. Production of high yields of docosahexaenoic acid by thraustochytrium roseum. ATCC 28210. Journal of Industrial Microbiology. 16:370-373.
Sparrow, F. K. , Jr. 1960. Aquatic Phycomycetes. 2nd ed. pp828-832.
Sparrow, F. K. , Jr. 1936. Biological observations on the marine fungi oof woods hole water. Biol. Bull. 70:236-263.
Ulken, A. 1965. Zwei neue thraustochytrien aus der aubenweser. Veroff. Inst. Meeresforsch. Bremerh. 9:289-296.
Ulken, A. 1968. Estimation of chytrid propagules in Douglas Lake by the MPN=pollen grain method.
Ulken, A. 1981. The phycomycete flora of Mangrove Swamps in the South Pacific. Veroff. Inst. Meeresforsch. Bremerh. 19:45-59.
Ulken, A., and F. O. Sparrow. 1986. Estimation of thraustochytrid propagules in two mangrove swamps. Bot. Mar. 29:85-89.
Vishniac, H. S. 1960. Salt requirements of marine phycomycetes. Limnol. Oceanogr. 5:362-365.
Vishniac, H. S. , G. A. Riley. 1961. Cobalamin and thiamine in Long Island Sound : patterns of distribution and ecological significance. Limnol. Oceanogr. 6:36-41.
.Volz, P. A. , Y.C. Hsu , and C.H. Liu. 1976. The Thraustochytriceae and other intertidal fungi of Taiwan. Taiwania 21:1-5.
Weete, J. D., H. Kim, S. R. Gandhi, Y. Wang, and R. Dute. 1997. Lipids and ultrastructure of thraustochytrium sp. ATCC 26185. Lipids 32(8):839-845.
羅婉 .1991。台灣產數種破囊壺菌形態、分類學上之研究。國立台灣師範大學生物研究所碩士班論文，共六十五頁。
許美蓮.1992。陽明山國家公園壺菌目形態、分類學之研究。國立台灣師範大學生物研究所碩士班論文，共九十五頁。