選取台灣產的網柱細胞黏菌屬(Dictyostelium)純培養菌株，分別為布列菲氏網柱細胞黏菌(Dictyostelium brefeldianum Hagiwara)、棍棒頭網柱細胞黏菌(D. clavatum Hagiwara)、纖細網柱細胞黏菌(D. delicatum Hagiwara)、微小型網柱細胞黏菌(D. exigumm Hagiwara)、細小網柱細胞黏菌(D. minutum Raper)、巨網柱細胞黏菌(D. giganteum Singh)和紫色網柱細胞黏菌(D. purpureum Olive ) 共七種，另和日本學者所贈與之日本地區六種網柱細胞黏菌為實驗材料，分別從這些菌株抽取出Genomic DNA，並利用聚合酶連鎖反應法及適當引子，大幅得到網柱細胞黏菌的核醣體DNA (rDNA)之內部轉錄間隔區(ITS)及5.8S(ITS1-5.8S-ITS2)核酸序列。將各菌株之核酸序列進行分析，並引用GenBank所登錄之七種網柱細胞黏菌rDNA核酸序列，經排序(alignment)後，再以MEGA 2、PAUP 4.0等軟體，繪出演化樹狀圖。結果顯示網柱細胞黏菌屬具有極粒(polar granule)孢子和不具有極粒孢子之兩群物種，可區分出來。另以5.8S核酸序列分析網柱細胞黏菌和其他生物之間的親緣關係，顯示網柱細胞黏菌與原生生物界親緣關係較接近，而與動物界、植物界、和真菌界親緣關係較遠。

Seven dictyostelid isolates from Taiwan, namely: Dictyostelium brefeldianum Hagiwara, D. clavatum Hagiwara, D. delicatum Hagiwara, D. exigumm Hagiwara, D. minutum Raper, D. giganteum Singh, and D. purpureum Olive together with six reference strains from Japan were used in this study. Genomic DNA were extracted from pure cultures, then the ITS and 5.8S nuclear ribosomal DNA (rDNA) were amplified by polymerase chain reaction (PCR) with two pairs of designed primers. The rDNA sequences of tested isolates were analysis with seven rDNA sequences of Dictyostelium registered in NCBI. Sequences were aligned by Clustal X, and the phylogenetic relationship tree was constructed by MEGA 2 and PAUP 4.0 software. The results were shown as follows: Dictyostelids with or without polar granules can be divided into two groups and phyologenetic analysis of 5.8S rDNA sequence, Dictyostelium species were closely related with Kingdom Protista, but far away from Kingdom Animalia, Plantae, and Fungi.

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