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研究生: 李宜庭
論文名稱: 人體揮發性有機物及飲食導致呼氣丙酮變化之研究
An Investigation on Volatile Organic Compounds from Human Body and Diet Influence on Breath Acetone
指導教授: 呂家榮
Lu, Chia-Jung
學位類別: 碩士
Master
系所名稱: 化學系
Department of Chemistry
論文出版年: 2020
畢業學年度: 108
語文別: 中文
論文頁數: 86
中文關鍵詞: 人體揮發性有機物呼氣丙酮micro-GC非侵入式檢測
英文關鍵詞: human VOCs, breath acetone, micro-GC, non-invasive test
DOI URL: http://doi.org/10.6345/NTNU202000870
論文種類: 學術論文
相關次數: 點閱:216下載:0
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  • 人體的代謝物一定程度上的反應了人體的生體狀態,而近幾年來,越來越來多學者在人體的揮發性有機化合物(VOCs)上進行了研究,其中,呼出氣體的分析為大宗。由於它的非侵入性和方便採樣的特點,使它具有在不同領域下運用的潛能。
    本論文主要是研究並排除外界的干擾,開發人體VOCs的採樣方式,並利用自行組裝的前濃縮系統進行分析物的收集,以氣相層析質譜儀來進行分析。本研究鑑定出50種以上在人體呼出氣體中的化合物,同時配合環境的檢測,比對呼出氣體和環境氣體的相關性。發現呼出氣體中烯類、醇類、酮類和含硫化合物較環境濃度高,而其中含硫類化合物主要來自飲食。而烷類、醛類、酯類、含苯化合物則是環境中含量較高。而在手心皮膚表面能夠偵測出15種左右的的化合物。另外,利用實驗室之前開發的微氣相層析儀能夠快速簡便的達成特定化合物的連續分析,在本研究中用來檢測不同飲食對人體呼氣丙酮濃度隨時間變化的趨勢,對五位健康的受試者進行測試,發現呼出丙酮的濃度會隨著攝取的食物而發生改變,它能一定程度上的反應人體的代謝狀態。

    One’s metabolites can reflect the state of himself in certain degree. In recent year, more and more researchers have conducted the research in human’s volatile organic compounds (VOCs), especially in breath analysis. Because its non-invasion and convenience, make it full of the potential in different area.
    This thesis aims to study and eliminate the interference when collecting the breath sample, develop a new method to sample human’s VOCs and collect the analytes by self -assembly preconcentrate system, analyze by Gas chromatography–mass spectrometry. With the method in this research, over 50 compounds in human breath can be detected. Also, by detecting the environment air to compare with the breath component. In these compounds, alkanes, alcohols, and ketones are found with higher concentration in human breath, whilst alkanes, aldehydes, esters and aromatic compounds have higher concentration in environment. In hand palm VOCs analysis, about 15 compounds can be found. Meanwhile, the influence of food intake on breath acetone was analysis by lab-made micro-GC, which can be used to analyze certain compound continuously. By analyzing five healthy subjects, finds out that the concentration of breath acetone changes after the food intake, which means breath acetone can reflect the metabolism in human in a certain way.

    摘要 I Abstract II 目錄 III 表目錄 VI 圖目錄 VIII 第一章 緒論 1 1.1. 前言 1 1.2. 人體呼吸作用和其所在的環境 4 1.3. 環境中的揮發性有機物 8 1.4. 呼氣中揮發性有機物的來源與其生理意義 10 1.4.1. 氨 11 1.4.2. 丙酮 12 1.4.3. 異戊二烯 15 1.4.4. 異丙醇 16 1.5. 人體皮膚揮發性有機物 18 1.6. 人體揮發性有機物採集方式 20 第二章 實驗部分 22 2.1. 實驗藥品與器材 22 2.1.1. 實驗藥品 22 2.1.2. 實驗儀器與器材 23 2.2. 前濃縮系統 24 2.2.1. 前濃縮系統介紹和其改良 24 2.2.2. 六向閥狀態 25 2.2.3. 前濃縮管 27 2.2.4. 前濃縮系統使用流程 29 2.3. 氣相層析-質譜儀 30 2.4. 採樣方式 31 2.4.1. 呼氣中揮發性有機物 31 2.4.2. 手心揮發性有機物 35 第三章 結果與討論 39 3.1. 採樣參數 39 3.1.1. 吸附劑效果比較 39 3.1.2. 氣體來源 40 3.1.3. 採樣袋與玻璃集氣管 41 3.1.4. 玻璃集氣管回收率 43 3.2. 手心揮發性有機物 44 3.3. 環境背景與呼氣中揮發性有機物 48 3.4. 食物與呼出丙酮 69 第四章 結果 78 參考資料 80

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