吳茱萸為常用之中藥材，主要含有生物鹼、精油、有機酸及苦味素。藥理
研究顯示吳茱萸的生物鹼具有抗菌、鎮痛、強心、抗心律不整、保持體溫
等作用。吳茱萸與黃連配伍併用，可治脅肋疼痛、脘痛吞酸、嘔惡嘈雜等
證。疏肝湯是具黃連吳茱萸藥對組成的方劑之一，適用於左脅腹痛、肋間
神經痛、跌打脅痛、胰臟炎等症狀。本研究以毛細管電泳測定吳茱萸、黃
連吳茱萸藥對及疏肝湯製劑之組成成分的含量，並評估該分析方法之適宜
性。藉由膠束電動力學毛細管層析(MECC)及毛細管區帶電泳(CZE)的使用
可成功的分離吳茱萸中的十個生物鹼成分。在MECC中可在十五分鐘內判時
分析evodiamine, rutaecarpine, carboxyevodiamine, 1-methyl-2-
nonyl-4(1H)-quinolone, 1-methyl-2-[(Z)-6-undecenyl]-4(1H)-
quinolone, 1-methyl-2-undecyl-4(1H)-quinolone, evocarpine,1-
methyl-2-[(6Z,9Z)-6,9-pentadeca-dienyl]-4(1H)-quinolone及
dihydro-evocarpine，而在CZE則於五分鐘之內定量dehydroevodiamine。
各成分之偵測極限範圍在35~47ug/ml，相對標準偏差低於4%。利用添加
maleic acid至吳茱萸的CZE系統，使得黃連吳茱萸藥對中的八個四級銨鹽
生物鹼(黃連的coptisine, berberine,berberastine, epiberberine,
columbamine, jatrorrhizine,palmatine及吳茱萸的dehydroevodiamine)
可以在二十五分鐘內同時分析。再配合吳茱萸的MECC條件，則總共可定量
藥對中的十七個成分。各成分之回收率於MECC條件下在96.68%-103.19%之
間；而在CZE下則在99.65%-103.28%之間。相對標準偏差於MECC條件下
在1.67%-4.00%之間；而在CZE下則在2.33%-4.38%之間。由於毛細管電泳
的高選擇性，藉著使用三種不同的分析條件可以分析出疏肝湯中的十八個
化合物。在MECC中可同時分析paeoniflorine,benzoic acid,
neohesperidin, hesperidin, naringin, ligustilide,
butylidenephthalide, umbelliferone；在CZE中分析
dehydroevodiamine, coptisine, berberine, epiberberine,
columbamine, jatrorrhizine, palmatine；而以逆電滲流毛細管區帶電
泳(reversed electroosmotic CZE)分析nicotinic acid, caffeicacid
和ferulic acid三個成分。其分析時間分別為–MECC: 40分鐘；standard
CZE: 30分鐘；reversed EOF CZE: 11分鐘。

E. rutaecarpa is a widely used Chinese herbal drug whichcontains
alkaloids, essential oils, carboxylic acids andlimonoids as its
main components. According toPharmacological tests, the
alkaloids were found to haveantifungaling, analgesia,
cardiotonic and body-temperature maintaining effects. The
combined use ofCoptidis Rhizoma and Evodiae Fructus enable the
healingof hypochondric and costal pain, stomachache,
acidregurgitation, nausea and gastric upset. Shu-kan-tang,
aformula which contains coptis-evodia herb couple, isused to
cure the pain in the lower left ribs,intercostal neuralgia,
costal pains due to confusion andpancreatitis. In this research
the contents of thecomponents in Evodiae Fructus, coptis-evodia
herb coupleand Shu-kan-tang were determined by
capillaryelectrophoresis (CE) and the system suitabilities
ofthose proposed methods were also evaluted.A total of ten
evodia alkaloids was separated bymicellar electrokinetic
capillary chromatography (MECC)and capillary zone
electrophoresis (CZE). The MECCmethod was applied to analyze
evodiamine, rutaecarpine,carboxyevodiamine, 1-methyl-2-nonyl-4(1
H)-quinolone, 1-methyl-2-[(Z)-6-undecenyl]-4(1H)-quinolone,
1-methyl-2-undecyl-4(1H)-quinolone, evocarpine, 1-methyl-2-[(6
Z,9Z)-6,9-pentadecadienyl]-4(1H)-quinolone anddihydroevocarpine
in 15 min, and CZE technique was usedto determine the
dehydroevodiamine in 5 min. Limits ofdetection for the alkaloids
were in the range of 35-47 ug/ml. The relative standard
deviations were less than 4%(n=6).With the addition of maleic
acid to evodia's CZE system,eight quaternary alkaloids in
coptis-evodia herb couple(containing dehydroevodiamine in
evodia, and coptisine,berberine, berberastine, epiberberine,
columbamine,jatrorrhizine, palmatine in coptis) could be
analyzedsimultaneously within 25 min. Combing this CZE
techniquewith the evodia's MECC method, a total of
seventeenalkaloids in coptis-evodia herb couple were determined.
The recoveries were 96.68-103.19% in MECC and 99.65-103.28% in
CZE, with a relative standard deviation of1.67-4.00% for MECC
and 2.33-4.38% for CZE.Eighteen components in Shu-kan-tang were
separated bycombining three different analytical methods. The
MECCmethod was applied to analyze paeoniflorine, benzoicacid,
neohesperidin, hesperidin, naringin, ligustilide,
butylidenephthalide and umbelliferone in 40 min; CZEtechnique
was used to determine coptisine,dehydroevodiamine, berberine,
epiberberine, columbamine,jatrorrhizine, palmatine in coptis in
30 min; andreversed EOF CZE was used to analyzed nicotinic acid,
caffeic acid and ferulic acid in 11 min.
E. rutaecarpa is a widely used Chinese herbal drug whichcontains