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研究生: 李香瑩
Hsiang-Ying Li
論文名稱: 台灣產唐松草屬之親緣關係與傅氏唐松草複合群之系統分類
Phylogeny of the genus Thalictrum (Ranunculaceae) in Taiwan and systematics of Thalictrum urbaini complex
指導教授: 王震哲
Wang, Jenn-Che
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2011
畢業學年度: 99
語文別: 中文
論文頁數: 102
中文關鍵詞: 毛茛科唐松草屬親緣關係系統分類
英文關鍵詞: Ranunculaceae, Thalictrum, phylogeny, systematics
論文種類: 學術論文
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  • 台灣產唐松草屬(Thalictrum)植物根據台灣植物誌第二版處理成六個分類群,除了T. sessile外,其餘類群在中國植物志的處理中分屬叉枝唐松草組(Sect. Leptostigma)及唐松草組(Sect. Tripterium)。本研究從外部形態、生態與地理分布及分子親緣分析等各方面對台灣產唐松草屬植物重新進行分類研究。綜合結果顯示台灣植物誌紀錄為微毛爪哇唐松草的類群為一新種,暫定名為能高唐松草(Thalictrum nokoensis H. Y. Li sp. nov)。利用核DNA ITS片段及兩個葉綠體DNA matK、trnL-F片段之分子序列資料所建構之本屬植物親緣關係樹,結果顯示台灣的類群可以分為兩支序,一個支序包含T. rubescens、T. urbaini var. urbaini、T. urbaini var. majus,此三者在分子上關係不明,但形值分析結果顯示萼片長、果柄長和瘦果長為三者主要的區分特徵;另一支序則包含其餘類群,此一結果不支持中國植物誌在屬下階層的處理,顯示本屬依形態建構的屬下分類系統有進一步檢討的必要。
    根據第一部份結果,本研究第二部份中將T. rubescens、T. urbaini var. urbaini、T. urbaini var. majus合稱為傅氏唐松草複合群,利用分子標記探討此複合群的遺傳分化程度。由核DNA合併三個葉綠體DNA片段所獲得的單套型來看,其分布模式與地理位置不具相關性。最小關聯網狀圖(minimum spanning network)顯示傅氏唐松草複合群之單套型彼此均為姐妹型(sister haplotype),加上不同類群擁有相同的基因型,本研究推論本複合群可能在近期產生分化,尚處於支序排列不完全(lineage sorting incomplete)的狀態。

    Six taxa of the genus Thalictrum are recognized in Flora of Taiwan 2nd-ed. Excluding T. sessile, two taxa are classified into Sect. Leptostigma and three into Sect. Tripterium in Flora of China Chinese Ed.. In this study, the genus Thalictrum is taxonomically revised based on morphological, geographical, ecological and molecular data. According to the result, T. javanicm var. puberulum is revised as a new species named Thalictrum nokoensis H. Y. Li sp. nov. To examine the phylogenetic relationship of the genus, DNA sequences from nrDNA ITS and two chloroplast DNA intergenic spacers, matK and trnL-F were analyzed. The phylogenetic tree reveals that the taxa in Taiwan are grouped into two clades. One clade consists of T. rubescens、T. urbaini var. urbaini and T. urbaini var. majus although their relationships are still unsolved. Results from morphometrics suggest that the major distinguishing characters are sepal, fruit stalk and achene length. The other clade comprises the remainding taxa. The incongruence between molecular clusters and the infrageneric system of Flora of China suggests that a reappraisal of the infrageneric system is necessary.
    According to the results of part one, T. urbaini var. urbaini, T. urbaini var. majus together with T. rubescens are defined as T. urbaini complex here. Highly similar morphology and closely-related phylogenetic relationships are found in the T. urbaini complex with differentiation in habitats and altitudinal distribution. In this study, data based on sequences of nrDNA ITS and three chloroplast DNA fragments describing genetic differentiation of the complex and the analysis of minimum spanning network reveal that haplotypes comprise sister haplotypes, and that the distribution of haplotypes in T. urbaini complex doesn’t have geographical structure. In addition, some different taxa have the same genotype. This could be due to recent differentiation and/or ancient incomplete lineage sorting.

    目次 目次 I 附表目次 IV 附圖目次 V 中文摘要 VI 英文摘要 VII 第一部份:台灣產唐松草屬之親緣關係 壹、前言 2 一、台灣產唐松草屬之分類歷史及研究背景 2 二、唐松草屬之分子研究 6 三、台灣島的地質歷史與特殊的地理位置 7 四、研究的問題 8 貳、研究材料與方法 9 一、研究材料 9 二、研究方法 11 参、結果 16 一、形態特徵 16 二、地理分布 17 三、物候 20 四、唐松草亞科之親緣關係 21 五、台灣產唐松草屬之親緣關係 26 肆、分類處理 31 伍、討論 54 一、親緣關係 54 二、與Yang & Huang (1989)形態研究結果比較 55 陸、參考文獻 56 第二部份:傅氏唐松草複合群之系統分類 壹、前言 62 一、傅氏唐松草複合群分類歷史及形態概述 62 二、複合群研究背景 62 三、親緣地理學研究現況 63 四、族群遺傳結構與網狀親緣圖之建立 64 五、研究的問題 65 貳、研究材料與方法 67 一、研究材料 67 二、研究方法 69 参、結果 75 一、遺傳組成 75 二、最小關連網狀圖(Minimum spanning network) 76 三、單套型(Haplotype)在族群中的組成與分布 78 四、中性假說檢測 82 五、族群分化指數(Fst)與基因交流指數(Nm) 82 六、距離隔離分化檢測(Isolation by distance test) 87 七、族群變異分布分析(Mismatch distribution) 87 八、巢狀支序分析(Nested clade phylogeographic analysis) 87 肆、討論 91 一、單套型之地理分布探討 91 二、傅氏唐松草複合群之族群變動歷史 93 三、傅氏唐松草複合群族群分化之探討 93 四、綜合形態與分子資料之討論 94 伍、參考文獻 96 附錄 一、唐松草亞科各屬之線上序列下載資訊 100 附錄 二、區別分析測量特徵項目 101 附錄 三、微毛爪哇唐松草模式標本照片 102 附表目次 表 1- 1、本研究唐松草屬中英學名對照表 4 表 1- 2、台灣產唐松草屬植物學名沿革表 5 表 1- 3、本研究檢視唐松草屬植物模式標本一覽表 10 表 1- 4、核DNA及葉綠體DNA引子序列資訊 13 表 1- 5、PCR反應試藥與濃度 13 表 1- 6、台灣產唐松草屬植物之物候調查 20 表 1- 7、T. javanicum var. puberulum與T. nokoensis比較 35 表 1- 8、T. myriophyllum、T. sessile、T. nokoensis比較 38 表 1- 9、T. sessile與T. nokoensis比較 43 表 1- 10、區別分析所能區別的樣本比例 51 表 1- 11、各特徵在區別分析中之特徵向量 51 表 1- 12、區別分析之錯分個體統計表 52 表 2- 1、傅氏唐松草複合群生育環境及形態特徵比較表 66 表 2- 2、採集地點之地理資訊 67 表 2- 3、核DNA及葉綠體DNA引子序列資訊 70 表 2- 4、PCR反應試藥與濃度 71 表 2- 5、傅氏唐松草複合群各單套型在族群中的數量(nrDNA) 79 表 2- 6、傅氏唐松草複合群各單套型在族群中的數量(cpDNA) 79 表 2- 7、傅氏唐松草複合群各片段遺傳變異分析 82 表 2- 8、傅氏唐松草複合群以形態區分之各族群遺傳分化指數及基因流傳指數(nrDNA) 84 表 2- 9、傅氏唐松草複合群以形態區分之各族群遺傳分化指數及基因流傳指數(cpDNA) 84 表 2- 10、傅氏唐松草複合群各地理族群遺傳分化指數及基因交流指數(nrDNA) 85 表 2- 11、傅氏唐松草複合群各地理族群遺傳分化指數及基因交流指數(cpDNA) 86 表 2- 12、傅氏唐松草複合群之NCPA分析結果 90 附圖目次 圖 1- 1、唐松草屬依型態建構之屬下分類系統 4 圖 1- 2、台灣產唐松草屬植物之地理分布圖 18 圖 1- 3、利用核DNA的ITS片段,以MP建構唐松草亞科之親緣關係 22 圖 1- 4、利用核DNA的ITS片段,以NJ建構唐松草亞科之親緣關係 23 圖 1- 5、利用核DNA的ITS片段,以MP建構唐松草亞科之親緣關係 24 圖 1- 6、利用核DNA的ITS片段,以NJ建構唐松草亞科之親緣關係 25 圖 1- 7、利用核DNA的ITS片段,以MP建構台灣產唐松草屬植物之親緣關係 27 圖 1- 8、利用核DNA的ITS片段,以NJ建構台灣產唐松草屬植物之親緣關係 28 圖 1- 9、利用葉綠體DNA的matK、trnL-F(包含trnL intron)片段,以MP建構台灣產唐松草屬植物之親緣關係 29 圖 1- 10、利用葉綠體DNA的matK、trnL-F(包含trnL intron)片段,以NJ建構台灣產唐松草屬植物之親緣關係 30 圖 1- 11、形值分析之區別分析座標圖 52 圖 2- 1、傅氏唐松草複合群之採集地點 68 圖 2- 2、傅氏唐松草複合群核DNA單套型之最小關連網狀圖 76 圖 2- 3、傅氏唐松草複合群合併葉綠體DNA單套型之最小關連網狀圖 77 圖 2- 4、傅氏唐松草複合群核DNA各單套型在各族群數量之圓餅圖 80 圖 2- 5、傅氏唐松草複合群葉綠體DNA各單套型在各族群數量之圓餅圖 81 圖 2- 6、傅氏唐松草複合群各族群地理距離與遺傳距離相關性測試圖 88 圖 2- 7、傅氏唐松草複合群族群變異分析檢測圖 89 圖 2- 8、傅氏唐松草複合群以葉綠體DNA建構之親源網狀圖 89

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