研究生: |
李香瑩 Hsiang-Ying Li |
---|---|
論文名稱: |
台灣產唐松草屬之親緣關係與傅氏唐松草複合群之系統分類 Phylogeny of the genus Thalictrum (Ranunculaceae) in Taiwan and systematics of Thalictrum urbaini complex |
指導教授: |
王震哲
Wang, Jenn-Che |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
論文出版年: | 2011 |
畢業學年度: | 99 |
語文別: | 中文 |
論文頁數: | 102 |
中文關鍵詞: | 毛茛科 、唐松草屬 、親緣關係 、系統分類 |
英文關鍵詞: | Ranunculaceae, Thalictrum, phylogeny, systematics |
論文種類: | 學術論文 |
相關次數: | 點閱:141 下載:9 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
台灣產唐松草屬(Thalictrum)植物根據台灣植物誌第二版處理成六個分類群,除了T. sessile外,其餘類群在中國植物志的處理中分屬叉枝唐松草組(Sect. Leptostigma)及唐松草組(Sect. Tripterium)。本研究從外部形態、生態與地理分布及分子親緣分析等各方面對台灣產唐松草屬植物重新進行分類研究。綜合結果顯示台灣植物誌紀錄為微毛爪哇唐松草的類群為一新種,暫定名為能高唐松草(Thalictrum nokoensis H. Y. Li sp. nov)。利用核DNA ITS片段及兩個葉綠體DNA matK、trnL-F片段之分子序列資料所建構之本屬植物親緣關係樹,結果顯示台灣的類群可以分為兩支序,一個支序包含T. rubescens、T. urbaini var. urbaini、T. urbaini var. majus,此三者在分子上關係不明,但形值分析結果顯示萼片長、果柄長和瘦果長為三者主要的區分特徵;另一支序則包含其餘類群,此一結果不支持中國植物誌在屬下階層的處理,顯示本屬依形態建構的屬下分類系統有進一步檢討的必要。
根據第一部份結果,本研究第二部份中將T. rubescens、T. urbaini var. urbaini、T. urbaini var. majus合稱為傅氏唐松草複合群,利用分子標記探討此複合群的遺傳分化程度。由核DNA合併三個葉綠體DNA片段所獲得的單套型來看,其分布模式與地理位置不具相關性。最小關聯網狀圖(minimum spanning network)顯示傅氏唐松草複合群之單套型彼此均為姐妹型(sister haplotype),加上不同類群擁有相同的基因型,本研究推論本複合群可能在近期產生分化,尚處於支序排列不完全(lineage sorting incomplete)的狀態。
Six taxa of the genus Thalictrum are recognized in Flora of Taiwan 2nd-ed. Excluding T. sessile, two taxa are classified into Sect. Leptostigma and three into Sect. Tripterium in Flora of China Chinese Ed.. In this study, the genus Thalictrum is taxonomically revised based on morphological, geographical, ecological and molecular data. According to the result, T. javanicm var. puberulum is revised as a new species named Thalictrum nokoensis H. Y. Li sp. nov. To examine the phylogenetic relationship of the genus, DNA sequences from nrDNA ITS and two chloroplast DNA intergenic spacers, matK and trnL-F were analyzed. The phylogenetic tree reveals that the taxa in Taiwan are grouped into two clades. One clade consists of T. rubescens、T. urbaini var. urbaini and T. urbaini var. majus although their relationships are still unsolved. Results from morphometrics suggest that the major distinguishing characters are sepal, fruit stalk and achene length. The other clade comprises the remainding taxa. The incongruence between molecular clusters and the infrageneric system of Flora of China suggests that a reappraisal of the infrageneric system is necessary.
According to the results of part one, T. urbaini var. urbaini, T. urbaini var. majus together with T. rubescens are defined as T. urbaini complex here. Highly similar morphology and closely-related phylogenetic relationships are found in the T. urbaini complex with differentiation in habitats and altitudinal distribution. In this study, data based on sequences of nrDNA ITS and three chloroplast DNA fragments describing genetic differentiation of the complex and the analysis of minimum spanning network reveal that haplotypes comprise sister haplotypes, and that the distribution of haplotypes in T. urbaini complex doesn’t have geographical structure. In addition, some different taxa have the same genotype. This could be due to recent differentiation and/or ancient incomplete lineage sorting.
Boivin, B. 1957. Études Thalietrologiques III. Réduction dugenre Anemonella Spach (Ranunculaceae). Bull. Soc. Roy. Bot. Belgique 89: 319–321.
Cai,Y.F., S.W. Li, Y. Liu, S. Quan, M. Chen, Y.F. Xie, H.Z. Jiang, E.Z. Wei, N.W. Yin, L. Wang, R. Zhang, C.L. Huang, X.H. He and M.F. Jiang, 1997. Molecular phylogeny of Ranunculaceae based on internal transcribed spacer sequences. Afr. J. Biotech. 8(20):5215-5224
Chen, Z.D., X.Q. Wang, H.Y. Sun, Y. Han, Z.X. Zhang, Y.P. Zou and A.M. Lu, 1998. Systematic position of the Rhoipteleaceae: evidence from nucleotide sequences of the rbcL gene. Acta Phytotax. Sin. 36: 1–7.
Cheng, Y.P., S.Y. Hwang, T.P. Lin, 2005. Potential refugia in Taiwan revealed by the phylogeographical study of Castanopsis carlesii Hayata (Fagaceae). Mol. Ecol. 14: 2075–2085.
Chiang, Y.C., K.H Hung., B.A. Schaal, X.J. Ge, T.W. Hsu and T.Y. Chiang, 2006. Contrasting phylogeographical patterns between mainland and island taxa of the Pinus luchuensis complex. Mol. Ecol. 15: 765–779.
Chung, J.D., T.P. Lin, Y.C. Tan, M.Y. Lin, S.Y. Hwang, 2004. Genetic diversity and biogeography of Cunninghamia konishii (Cupressaceae), an island species in Taiwan: a comparison with Cunninghamia lanceolata, a mainland species in China. Mo. Phylogenet. Evol. 33: 791–801.
Fu, D.Z. and G.H. Zhu, 2001. Thalictrum. Flora of China 6: 282–302.
Ge, X.J., X.L. Zhou, Z.C. Li, T.W. Hsu, B.A. Schaal and T.Y. Chiang, 2005. Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting. J. Plant Res. 118: 415–422.
Hall, T. A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95-98.
Hayata, B., 1911. Ranunculaceae. Icon. Pl. Formos. 1: 25.
Hayata, B., 1913. Ranunculaceae. Icon. Pl. Formos. 3: 4-6. Pl. 1 & f. 2.
Huang, S., Y.C. Chiang, B.A. Schaal, C.H. Chou, T.Y. Chiang, 2001. Organelle DNA phylogeography of Cycas taitungensis, a relict species in Taiwan. Mol. Ecol. 10: 2669–2681.
Huang, S.F. and T.P. Lin, 2006. Migration of Trochodendron aralioides (Trochodendraceae) in Taiwan and its adjacent areas. Botanical Studies 47: 83–88.
Huang, S.S., 1960. Preliminary notes on the Ranunculaceae of Taiwan (Formosa), China. Biol. Bull. Nat. Taiwan Norm. Univ. 5: 46-50.
Koidzumi, G., 1925. Contriburiones ad Cognitionem florae Asiae Orientalis, XII. Bot. Mag. Tokyo 39: 19-20
Kumar, S., J. Dudley. M. Nei. and K. Tamura, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596-1599.
Liu, T.S. and C.F. Hsieh. 1976. Ranunculaceae. In: Li, H.-L. et al. (eds.). Flora of Taiwan. Vol. 2. Epoch Publ. Co., Taipei, Taiwania. pp. 508-513.
Lu, S.Y., C.I. Peng, Y.P. Cheng, K.H. Hong and T.Y. Chiang, 2001. Chloroplast DNA phylogeography of Cunninghamia konishii (Cupressaceae), and endemic conifer of Taiwan. Genome 44: 797–807.
Marhold K. J. Lihová, M. Pern , R. Grupe and B. Neuffer, 2002. Natural hybridization in Cardamine (Brassicaceae) in the Pyrenees: evidence from morphological and molecular data. Bot. J. Linn. Soc. 139: 275-294
Masamune, G., 1934. On some noteworthy plants from the island of Formosa. J. Soc. Trop.Agr. 6: 569.
Matsumura, J. and B. Hayata, 1906. Enumeratio plantarum Formosanarum. J. Coll. Sci. Univ. Tokyo 22: 7. t. l.
McCauley, D.E. 1995. The use of chlorplast DNA polymorphism in studies of gene flow in plants. Trends Ecol. Evol. 10(5): 198-202.
Moller, M, amd Q. C. B. Cronk, 1997. Phylogeny amd disjunct distribution: evolution of Saintpaulia (Gesnerianceae): Proc. R. Soc. Lond. B. 264: 1827-1836.
Ohwi, J., 1933. Symbolae ad floram Asiae Orientalis, 9. Acta. Phytotax. Geobot. 2: 156-157.
Page, R. D. M. and E. C. Holmes. 1998. Molecular Evolution: a phylogenetic approach. pp. 73-77. Blackwell Publishing.
Park, M. M. and D. Festerling, 1997. Thalictrum. In: Flora of North America. Vol. 3
Posada, D. and K.A.Crandall, 1998. Modeltest: testing the model of DNA substitution. Bioinformatics 14 (9): 817-818.
Ro, K.E., C.S. Keener and B.A. McPheron, 1997. Molecular phylogenetic study of the Ranunculaceae: utility of the nuclear 26S ribosomal DNA in inferring intrafamilial relationships. Mol. Phylogenet. Evol. 8: 117-127.
Ro, K.E. and B.A. Mcpheron, 1997. Molecular phylogeny of the Aquilegia group (Ranunculaceae) based on internal transcribed spacers and 5.8S nuclear ribosomal DNA. Biochem. Syst. Ecol. 25: 445–461.
Saitou, N. and M. Nei., 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4): 406-425.
Sharma, A.K. and A. Sharma, 2005. Plant Genome-Biodiversity and Evolution. Science Publishers. pp. 57-65.
Shimizu, T., 1963. Studies on the limestone flora of Japan and Taiwan Part II. J. Fac. Tex. Sci. Technol., Shinshu Univ. n. 36, ser. A (Biol.), n. 12: 27-29
Swofford, D. L., 2002. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). In. Sinauer Associates, Inc., Sunderland, Massachusetts, USA.
Taberlet, P., L. Gielly, G. Pautou and J. Bouvet, 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Pl. Mol. Biol. 17: 1105-1109
Tamura, M., 1966. Morphology, ecology and phylogeny of the Ranunculaceae VI Sci. Rep. 15(1):13-16
Tamura, M. 1968. Morphology, ecology and phylogeny of the Ranunculaceae VIII. Sci. Rep. Coll. Gen. Educ. Osaka Univ. 17: 41–56.
Tamura, M. 1992. A new classification of the family Ranunculaceae 3. Acta Phytotax. Geobot. 43: 53–58.
Tamura, M. 1995. Ranunculaceae. pp. 441–494 in: Hiepko P. (ed.), Natürliche Pflanzenfamilien, 2nd ed. 17a IV. Duncker and Humblot, Berlin.
Tamura, M. & Kosuge, K. 1989. Classification of the Isopyroideae (Ranunculaceae). Acta Phytotax. Geobot. 40: 31–35.
Tsai, M. S., 1972. A Revision of Ranunculaceae of Taiwan. Master’s thesis of Department of Forest of National Taiwan University, Taipei. pp. 28-35
Yang, T.Y.A. and T.C. Huang., 1989. Notes on the flora of Taiwan (5): The Thalictrum Tournef. ex L. (Ranunculaceae). Taiwania 34: 58-73.
Yang, T.Y.A., T.C. Huang and J. A. Compton. 1996. Ranunculaceae L. In: Huang, T.-C. et al. (eds.), Flora of Taiwan 2nd ed. 2: 565-573, pls. 228-240. Editorial Committee, Dept. Bot., NTU, Taipei, Taiwan.
Yang, T.Y.A. and T.C. Huang., 2008. Additional Remarks on Ranunculaceae in Taiwan (8): Revision of Ranunculaceae in Taiwan. Taiwania 53(2): 210-229.
Wang, C.N., M. Moller and Q.C.B. Cronk, 2004. Population genetic structure of Titanotrichum oldhamii (Gesneriaceae), a subtropical bulbiliferous plant with mixed sexual and asexual reproduction. Ann. Bot. 93: 201–209.
Wang, W. and Z.D. Chen, 2007. Generic level phylogeny of Thalictroideae (Ranunculaceae)-implications for the taxonomic status of Paropyrum and petal evolution. Taxon 56(3): 811-821.
Wang, W., Z. D. Chen, Y. Liu, R. Q. Li, and J. H. Li, 2007. Phylogenetic and biogeographic diversification of Berberidaceae in the northern hemisphere. Syst. Bot. 32: 731–742.
Wang, W. T. and S. H. Wang, 1979. Thalictrum L. In W. T. et al. (eds.) Flora Reipublicae Popularis Sinicae V. 27. Science Press, Beijing. pp. 502-592 & 616-621.
Wei, X. X., Z. Y. Yang, Y. Li and X. Q. Wang, 2010. Molecular phylogeny and biogeography of Pseudotsuga (Pinaceae): Insights into the floristic relationship between Taiwan and its adjacent areas. Mol. Phylogenet. Evol. 55: 776–785.
Wolfe, K. H., W. H. Li, and P. M. Sharp. 1987. Rates of nucleotide substitution vary greatly among plant mitochondria chloroplast and nuclear DNAs. Proc. Natl. Acad. Sci. USA 84(24): 9054-9058
Avise, J. C., 1994. Molecular markers, natural history and evolution. Chapman and Hall, New York, USA.
Avise, J. C., J. Arnold. R. M. Ball. E. Bermingham Jr., T. Lamb. J. E. Neigel, C. A. Reeb, and N. C. Saunder, 1987. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst. 18: 489-522.
Clement, M., D. Posada. and K. A. Crandall., 2000. TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9: 1657-1659.
Davis, P. H. and V. H.Heywood, 1963. Principles of Angiosperm Taxonomy. Princeton NJ, Van Nostrand.
Fosberg, F. R., 1942. Subspecies and Variety. Rhodora: 153-157.
Friedman, J. and Spencer C. H. Barrett, 2009. Wind of change: new insights on the ecology and evolution of pollination and mating in wind-pollinated plants. Ann. Bot. 103: 1515-1527
Fu, Y.X. and W.H. Li., 1993. Maximum likelihood estimation of population parameters. Genetics 134: 1261-1270.
Grant, V., 1981. Plant Speciation, 2nd ed. Columbia University Press., New York, USA.
Grant, W. S. and B. W. Bowen., 1998. Shallow population histories in deep evolutionary lineages of marine fishes: insights from sardines and anchovies and lessons for conservation. Am. Genet. Assoc. 89(5):415-426.
Hall, T. A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41:95-98.
Hartl, D. L., A. G. Clark, 2007. Principles of population genetics. Sinauer Associates, Inc., Sunderland, Massachusetts. pp.95-148.
Hudson, R. R., M. Slatkin. and W. P. Maddison, 1992. Estimation of levels of gene flow from DNA sequence data. Genetics 132: 583-589
Jensen, J. L., A. J. Bohonak. and S. T. Kelley, 2005. Isolation by distance, web service. BMC Genetics 6:13.
Kimura, M., 1983. Rare veriant alleles in the light of the neutral theory. Mol. Biol. Evol. 1: 84-93.
Lovis, J.D., 1977. Evolutionary patterns and processes in ferns.Advances in Botanical Research 4: 229-415.
Moller, M, amd Q. C. B. Cronk, 1997. Phylogeny amd disjunct distribution: evolution of Saintpaulia (Gesnerianceae): Proc. R. Soc. Lond. B. 264: 1827-1836.
Marhold K., 1992. Multivariate morphometric study of the Cardamine amara group (Cruciferae) in the Carpathians and Sudeten mountains. Bot. J. Linn. Soc. 110: 121-135.
Marhold K., 1998. Morphometric comparison of diploid populations of Cardamine amara (Brassicaceae) from Central Europe and the Balkan Peninsula. Thaiszia. J. Bot. 8: 19–32.
Northington, D. K., 1976. Evidence bearing on the origin of infraspecific disjunction in Sophora gypsophlia (Fabaceae). Pl. Syst. Evol. 125: 233-244.
Posada D, K.A. Crandall and A.R. Templeton, 2000. GeoDis: A program for the Cladistic Nested Analysis of the Geographical Distribution of Genetic Haplotypes. Mol. Ecol. 9(4):487-488
Rogers, A. R. and H. Harpending, 1992. Population growth makes waves in the distribution of pairwise genetic differences. Mol. Biol. Evol. 9: 552-569.
Rozas, J., J. C. Sa'nchez-DelBarrio, X. Messegyer, and R. Rozas, 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19: 2496-2497.
Shaw J, E.B. Lickey, E.E. Schilling and R.L. Small, 2007. Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: The tortoise and the here III. Am.J. Bot. 94: 275-288.
Slatkin, M., 1987. Gene flow and the geographic structure of natural populations. Science 236: 787-792.
Stebbins, G. L., 1974. Flowering plants evolution above the spscies level. Belknap Press, Cambridge, Massachusetts, USA.
Steven, J.C. and D.M. Waller, 2004. Reproductive alternatives to insect pollination in four species of Thalictrum (Ranunculaceae). Pl. Species Biol. 19: 73-80.
Sylvester-Bradley, C, 1952. The Classification and Coordination of Infra-Specific Categories. Systematics Association, Mimeograph, London, p. 19.
Tajima, F., 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123: 585-595.
Templeton, A. R., 1998. Nested clade analysis of phylogeographic data: testing hypotheses about gene flow and population history. Mol. Ecol. 7:381-397.
Templeton, A. R., 2004. Statistical phylogeography: methods of evaluating and minimizing inference errors. Mol. Ecol. 13:789-809.
Templeton, A. R., E. Routman and C. A. Phillips, 1995. Separating population structure from population history: A cladistic analysis of the geographical distribution of mitochondrial DNA haplotypes in the tiger salamander, Ambystoma tigrinurn. Genetics 140:767-778.
Wang, W. and Z.D. Chen, 2007. Generic level phylogeny of Thalictroideae (Ranunculaceae)-implications for the taxonomic status of Paropyrum and petal evolution. Taxon 56(3): 811-821.
Wang, W. T. and S. H. Wang, 1979. Thalictrum L. In W. T. et al. (eds.) Flora Reipublicae Popularis Sinicae V. 27. Science Press, Beijing. pp. 502-592 & 616-621.
Wright, S., 1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19: 395-420