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研究生: 陳彥呈
Chen, Yan-Cheng
論文名稱: 低場核磁共振系統於乳癌組織檢測應用與特性研究
The Application and Characteristics in Breast Tumor Discrimination of High-Tc SQUID Based Low-field Nuclear Magnetic Resonance System
指導教授: 廖書賢
Liao, Shu-Hsien
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2018
畢業學年度: 106
語文別: 中文
論文頁數: 53
中文關鍵詞: 低場核磁共振系統高溫超導量子干涉元件乳癌組織檢測
英文關鍵詞: Low-Field NMR System, High-Tc SQUID, Breast Tumor Discrimination
DOI URL: http://doi.org/10.6345/THE.NTNU.EPST.010.2018.E08
論文種類: 學術論文
相關次數: 點閱:136下載:0
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  • 本實驗參考過去實驗室,研究低場核磁共振系統,使用超導量子干涉元件(SQUID, superconducting uantum interference device)和預極化(prepolarization)技術的低磁場核磁共振系統(Low-field NMR)應用至腫瘤量測上。改進接收線圈,使SNR提高20 % (量測0.5 ml去離子水),進而讓乳房樣品能有效進行微量(0.05 g ~ 0.2 g)量測,利用縱向鬆弛時間(T1)之物理方式量測,並進行乳房組織最小量測量與縮短實驗時間,乳房組織經由實驗後量測T1數值,統計資料證實,正常組織與腫瘤組織具有統計意義,並計算辨識正常組織與腫瘤組織的靈敏性(Sensitivity)與特異性(Specificity),具有高度的數值,同時證明使用T1量測,進行腫瘤組織檢測可行性。最後整合病理報告,核磁共振與病理報告內的細胞活化標記(Ki-67)、病理分期具有相關性。藉此能快速又有效提供可量化數值,提供醫師一種生物組織檢測方式。

    In this study, a high-Tc SQUID-detected nuclear magnetic resonance spectrometer was set-up in National Taiwan University Hospital to discriminate Breast tumor. Improve Pick-up coil and let SNR increase 20 percent (measure 0.5 ml di-water). Make breast samples effective for micro-measurement (0.05 g ~ 0.2 g). Exploit physical measurements of longitudinal relaxation time(T1)to measure breast samples and then measurement of the minimum sample size of breast tissue and shortening the time of the experiment. Statistics confirmed that normal tissues and tumor tissues are statistically significant, and the sensitivity and specificity of identifying normal tissues and tumor tissues are calculated, with high numerical values, and it is proved that tumor tissue is measured using T1 measurement. For tumor discrimination, the T1 relaxation time of normal tissue and tumor tissue were demonstrated. Besides, the relation between T1 of tumor tissue and KI-67, pathology grade was established.

    誌謝 I 摘要 II Abstract III 目錄 IV 表目錄 VI 圖目錄 VII 第1章 緒論 1 第2章 實驗原理 5 2.1 原子核特性 5 2.2 核磁共振原理 5 2.3 自由感應衰減 12 2.4 弛緩 ( Relaxation ) 14 2.4.1 T1 Relaxation縱向鬆弛 14 2.4.2 T2 Relaxation橫向鬆弛 15 2.5 Breast Cancer NMR 特性差異 16 2.6 磁通閘之頻率調整平均與時域頻率調整平均修正 19 第3章 實驗方法 20 3.1 樣品處理方式與實驗流程 20 3.2 T1 Relaxation time 量測 25 第4章 實驗結果 26 4.1 接收線圈最佳化 26 4.1.1 最小樣品量 30 4.1.2 樣品放置時間影響 33 4.1.3 樣品平均次數 36 4.1.4 Breast Cancer T1 value量測 39 4.1.5 病理報告整合 43 第5章 結論 50 參考資料 51

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