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研究生: 莊鈞評
論文名稱: 以磁減量免疫檢測與切片磁染色進行動物肝癌之體外檢測研究
ImmunoMagnetic Reduction and Magnetic Immunohistochemistry in vitro study of liver cancer in Rat
指導教授: 謝振傑
Chieh, Jen-Jie
廖書賢
Liao, Shu-Hsien
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2014
畢業學年度: 102
語文別: 中文
論文頁數: 35
中文關鍵詞: 磁染色IMR磁減量檢測磁流體肝癌老鼠
論文種類: 學術論文
相關次數: 點閱:125下載:7
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  • 目前醫院用來篩檢肝癌的方法為抽血檢驗,檢驗血清中的Alpha-fetoprotein(AFP),AFP的檢測靈敏度只有70%左右,但只憑AFP升高無法斷定罹患肝癌,還必須配合影像檢查,進行多種的檢驗來確認肝癌,多種檢驗造成效率低,固本研究研發披覆多重抗體試劑與混合試劑,以提高檢測之靈敏度。
    我們將肝癌細胞種植於老鼠背部,肝癌細胞從老鼠體內獲取養分,然後形成腫瘤,記錄腫瘤成長過程,並且按照種植肝癌細胞後的0、1、3、5週,量測血清內的肝癌指標性蛋白,目的是為了觀察腫瘤在前期與後期的指標性蛋白表現量,我們使用Alpha-fetoprotein(AFP)試劑、Glypican-3(GPC3)試劑、Des-gamma-carboxy prothrombin(DCP)試劑、披覆多重抗體試劑及混合試劑來檢測,使用的方法為磁減量檢測法(ImmunoMagnetic Reduction, IMR),在後期我們將腫瘤取出,做成切片,以磁性奈米試劑進行磁染色,成功的用磁訊號觀察腫瘤指標性蛋白。

    第一章 緒論 1 第二章 實驗原理 3 2-1 磁減量免疫檢測(ImmunoMagnetic Reduction ; IMR) 3 2-1 酵素免疫分析法(Enzyme-Linked Immunosorbent Assay;ELISA) 7 2-3 免疫染色(Immunohistochemistry;IHC) 8 2-4 磁染色 9 第三章 實驗方法 10 3-1磁性試劑製程 10 3-2 老鼠腫瘤的誘發 13 3-3 免疫染色與磁染色 15 3-4 以癌鼠血清進行IMR 18 3-5 以癌鼠血清進行ELISA 19 第四章 實驗結果 20 4-1 切片染色 20 4-2 以癌鼠血清進行IMR 24 4-3 以癌鼠血清進行ELISA 28 第五章 結論 30 參考文獻 31

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