研究生: |
蕭竹彣 Hsiao, Chu-Wen |
---|---|
論文名稱: |
以1-Thio-β-D-glucose修飾螢光金奈米團簇表面於標記腫瘤細胞之應用 Application of 1-Thio-β-D-glucose Modified Fluorescent Gold Nanoclusters for the Tumor Cells Imaging |
指導教授: |
陳家俊
Chen, Chia-Chun |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2016 |
畢業學年度: | 104 |
語文別: | 中文 |
論文頁數: | 79 |
中文關鍵詞: | 螢光金奈米團簇 、表面修飾 、葡萄糖轉運蛋白(GLUT1) 、1-Thio-β-D-glucose |
英文關鍵詞: | Fluorescent Gold Nanoclusters, Surface Modification, Glucose Transporter 1 (GLUT1), 1-Thio-β-D-glucose |
DOI URL: | https://doi.org/10.6345/NTNU202204485 |
論文種類: | 學術論文 |
相關次數: | 點閱:213 下載:0 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
螢光金奈米團簇(Gold Nanoclusters, Au NCs)因其良好的生物
相容性、易於進行表面修飾及其獨特的光學性質,被廣泛應用於生
物感測及生物成像。葡萄糖轉運蛋白1(GLUT1)為一種跨膜蛋白,負責運送葡萄糖在血管與細胞之進出。瓦式效應(Warburg effect)指腫瘤細胞即使在有氧的環境下也偏好透過糖解作用勝於氧化磷酸化來產生能量;在腫瘤細胞上,GLUT1會因為瓦式效應而在細胞膜上有大量表現。本實驗以螢光金奈米團簇標記腦瘤細胞,先以還原型的穀胱甘肽與四氯金酸合成出橘紅色的螢光金奈米團簇(OGS-Au NCs),再將1-Thio-β-D-glucose 修飾於螢光金奈米團簇表面,合成出表面接有葡萄糖之螢光金奈米團簇(TGlu-Au NCs),利用腫瘤細胞之瓦式效應,將接有葡萄糖之螢光金奈米團簇通過GLUT1送入腦瘤細胞,以達成標記腦瘤細胞之目的。在共軛焦顯微鏡所觀測到合成之TGlu-Au NCs能有效被人類腦神經膠質瘤細胞U-87 MG吸收。
Fluorescent gold nanoclusters have been widely employed in the biosensing and bioimaging because of their high biocompatibility, facile surface modification and unique optical properties. Glucose transporter 1 (GLUT-1) is a transmembrane protein which transport of glucose through cell membrane. Warburg Effect describes that tumor cells prefer to glycolysis rather than oxidative phosphorylation for energy production even in the presence of ample oxygen, tumor cells are increased in glucose uptake and elevated glycolysis. In this study, we prepared fluorescent gold nanoclusters and then the surface of fluorescent gold nanoclusters were modified with 1-Thio-β-D-glucose (TGlu-Au NCs). The TGlu-Au NCs showed very low cytotoxicity (the cell viability
> 90%). The confocal images show that the brain tumor cells of U-87 MG cells could uptake TGlu-Au NCs by GLUT1. To sum up, TGlu-Au NCs could be used as a potential fluorescent probe in the brain tumor cells.
參考文獻
[1] Underwood, S.; Mulvaney, P. Langmuir, 1994, 10 (10),
pp 3427–3430.
[2] British Museum - The Lycurgus Cup
http://www.britishmuseum.org/research/collection_online/collection_object_details.aspx?objectId=61219&partId=1&searchText=Lycurgus%20Cup
[3] Barber, D.J.; Freestone, I. C. Archaeometry 32(1),
1990, 33–45.
[4] Krpetic, Z.; Nativo, P.; Porta, F.; Brust, M.
Bioconjugate Chem. 2009, 20 (3), pp 619–624.
[5] Chen, L.-Y.; Huang, C.-C.; Chen, W.-Y.; Lin, H.-J.;
Chang, H.-T. Biosens. Bioelectron 2013, 43, 38-44.
[6] Huang, X.; El-Sayed, I. H.; Qian, W.; El-Sayed, M. A.
J. Am. Chem. Soc. 2006, 128 (6): 2115–2120.
[7] Murphy, C.J.; Gole, A.M.; Stone, J.W.; Sisco, P.N.;
Alkilany, A.M.; Goldsmith, E.C.; Baxter S.C. Acc.
Chem. Res. 2008, 41 (12): 1721–1730.
[8] Jain, P. K.; Huang, X.; El-Sayed, I. H.; El-Sayed, M.
A. Acc. Chem. Res. 2008, 41 (12): 1578–1586.
[9] Jun, Y.W.; Choi, J.S.; Cheon, J. Angew. Chem. Int.
Ed. 2006, 45 (21), 3414-3439.
[10] Kelly, K. L.; Coronado, E.; Zhao, L. L.; Schatz, G.
C., J. Phys. Chem. B 2003, 107 (3), 668-677.
[11] Nie, S.; Emory, S. R. Science 1997, 275, 1102-1106.
[12] Faraday, M. Philos. Trans. R. Soc. London 1857, 147,
145-181.
[13] Turkevich, J.; Stevenson, P.C.; Hillier, J. Discuss.
Faraday Soc. 1951, 11, 55-75.
[14] Frens, G. Nat . Phys. Sci. 1973, 241, 20-22.
[15] Jin, R. Nanoscale 2010, 2, 343-362.
[16] Zhang, L.; Wang, E. Nano Today 2014, 9, 132−157.
[17] Markus, R. ; Schwentner , N. Springer-Verlag: Berlin
1987, pp 611– 616.
[18] Fedrigo, S.; Harbich, W.; Buttet, J. J. Chem. Phys.
1993, 99 (8), 5712-5717.
[19] Mathew, A.; Pradeep, T. Part. Part. Syst. Charact
2014, 31 (10): 1017-1053.
[20] Maity, P.; Xie, S.; Yamauchi, M.; Tsukuda, T.
Nanoscale 2012, 4, 4027-4037.
[21] Templeton, A. C.; Wuelfing, M. P.; Murray, R. W.
Acc. Chem. Res. 2000, 33, 27-36.
[22] Schmid, G. Chem. Rev. 1992, 92 (8), 1709–1727.
[23] Brust, M.; Walker, M; Betthell, D.; Schiffrin, D.
J.; Whyman, R. J Chem. Soc., Chem. Commun. 1994,
801-802.
[24] Uehara, A.; Booth, S.G.; Chang, S.Y.; Schroeder,
S.L.M.; Imai, T.; Hashimoto, T.; Mosselmans, J.
Frederick W.; Dryfe, R.A.W. J. Am. Chem. Soc. 2015,
137 (48), pp 15135–15144.
[25] Hostetler, M. J.; Templeton, A. C.; Murray, R. W.
Langmuir 1999, 15, 3782-3789.
[26] Daniel, M.C.; Astruc, D. Chem. Rev. 2004, 104, 293-
346.
[27] Huang, B.; Bates, M.; Zhuang, X. Annu Rev Biochem.
2009, 78, 993-1016.
[28] Chan, W.C.; Nie, S. Science 1998, 281, 2016–2018.
[29] Sameiro, M.; Goncalves, T. Chem. Rev. 2009, 109,
190–212.
[30] Betzig, E.; Patterson, G. H.; Sougrat, R.;
Lindwasser, O. W.; Olenych, S.; Bonifacino, J. S.;
Davidson, M. W.; Lippincott-Schwartz, J., Hess, H.
F. Science 2006, 313, 1642-1645.
[31] Gao, X.; Yang, L.; Petros, J. A.; Marshall, F. F.;
Simons, J. W.; Nie, S. Curr. Opin. Biotechnol. 2005,
16, 63–72.
[32] Medintz, I. L.; Uyeda, H. T.; Goldman, E. R.;
Mattoussi, H. Nat Mater 2005, 4(6), 435–446.
[33] Xavier, P. L.; Chaudhari, K.; Baksi, A.; Pradeep, T.
Nano reviews 2012, 3, 14767_1-14767_16.
[34] Cha, S. H.; Kim, J. U.; Kim, K. H.; Lee, J. C. Chem.
Mater. 2007, 19, 6297−6303.
[35] Chen, L. Y.; Wang, C. W.; Yuan, Z. Q.; Chang, H. T.
Anal. Chem. 2015, 87, 216-229.
[36] Lin, C. A. J.; Lee, C. H.; Hsieh, J. T.; Wang, H.
H.; Li, J. K.; Shen, J. L.; Chan, W. H.; Yeh, H. I.;
Chang, W. H. J Med Biol Eng 2009, 29(6), 276-283.
[37] Zheng, J.; Petty, J. T.; Dickson, R. J. Am. Chem.
Soc. 2003, 125, 7780–7781.
[38] Xie, J.; Zheng, Y.; Ying, J. Y. J. Am. Chem. Soc.
2009, 131 (3), 888-889.
[39] Zhou, C.; Sun, C.; Yu, M.; Qin, Y.; Wang, J.; Kim,
M.; Zheng, J. J. Phys. Chem. C 2010, 114, 7727–7732.
[40] Chattoraj, S.; Bhattacharyy, K. J. Phys. Chem. C
2014, 118(38), 22339–22346.
[41] Li, C.H.; Kuo, T. R.; Su, H.J. Lai, W.Y.; Yang,
P.C.; Chen, J.S.; Wang, D.Y.; Wu, Y.C.; Chen, C.C.
Scientific Reports 2015, 5, 15675.
[42] Gillies, R.J.; Gatenby, R.A. Cancer Metas Rev, 2007,
26, 311-317.
[43] Warburg O. London: Arnold Constable, 1930, 254-270.
[44] Matthew G.; Vander Heiden; Lewis C. Cantley; Craig
B. Thompson Science 2009, 324(5930), 1029-1033.
[45] Berislav V. Zlokovic Nature Reviews Neuroscience
2011, 12, 723-738.