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研究生: 吳曉暢
Hsiao-Chang Wu
論文名稱: 規律運動配合硫辛酸增補對體內超氧離子歧化酶活性的影響
The Influence of Regular Exercise and Alpha Lipoic Acid Supplementation on the Superoxide Dismutase Activity in Human
指導教授: 謝伸裕
Hsieh, Shen-Yu
學位類別: 碩士
Master
系所名稱: 體育學系
Department of Physical Education
論文出版年: 2008
畢業學年度: 96
語文別: 中文
論文頁數: 47
中文關鍵詞: 規律運動硫辛酸超氧離子歧化酶氧化壓力
英文關鍵詞: regular exercise, alpha lipoic acid, SOD, oxidative stress
論文種類: 學術論文
相關次數: 點閱:144下載:8
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  • 目的:探討規律運動、抗氧化劑硫辛酸增補對體內超氧離子歧化酶活性的影響。方法:受試者共分為四組:服用安慰劑無規律運動習慣組(PN組)、服用安慰劑有規律運動習慣組(PE組)、增補硫辛酸無規律運動習慣增補組(AN組)及增補硫辛酸有規律運動習慣組(AE組),每組10位男性受試者,共計40人。研究中共進行2次衰竭性運動測試(Bruce potocol),兩次運動測試間隔2周予以增補硫辛酸或安慰劑之實驗處理,每次運動測試前、衰竭時、運動後15分鐘、運動後30分鐘分別採集血液樣本,分析血球超氧離子歧化酶活性。所得數據以混合設計雙因子變異數分析進行檢定。結果:第一次運動測試中,在衰竭及運動後15分鐘時,有規律運動習慣者(PE、AE組)血球SOD活性顯著高於無規律運動習慣者(PN、AN組);第二次運動測試中,衰竭時PE、AE組皆顯著高於PN、AN組;兩次運動測試間運動表現無顯著差異,SOD活性表現差異未達顯著;AE組與AN組第一次運動測試衰竭時無顯著差異(p = .079),第二次運動測試時則達顯著差異(p = .002),且AE組SOD活性峰值由運動後15分鐘前移至衰竭時,衰竭時SOD活性第二次運動測試顯著高於第一次(p = .035)。結論:連續兩週的抗氧化劑硫辛酸增補,對於有規律運動習慣者,雖無顯著SOD活性加成效果,但能加快體內SOD活化速率,來對抗體內氧化壓力。對於無規律運動習慣者,並沒有出現SOD顯著下降的激效作用情況。本研究中也再次印證規律運動可以有效提高體內SOD活性,增加人體抗氧化能力。建議未來在增補抗氧化劑的同時,應配合規律運動習慣,提高抗氧化能力的同時提早活化抗氧化酵素活性才能有效降低氧化壓力對身體造成的傷害。

    Abstract
    Purpose: To investigate the influence of regular exercise and antioxidant alpha lipoic acid (ALA) supplementation on superoxide dismutase (SOD) activity in human. Methods: Subjects were divided into four groups: (1) PN group: those who took placebo and had no regular exercise habits; (2) PE group: those who took placebo and had regular exercise habits; (3) AN group: those who took ALA and had no regular exercise habits; and (4) AE group: those who took ALA and had regular exercise habits. Ten male subjects in group with a total of forty subjects. Each subject had 2 exhausted exercise tests (Bruce protocol) and between the two tests, ALA or placebo was supplied. In each exercise test, in order to analyze SOD activity in blood cells, the blood samples were collected four times: (1) before the exercise; (2) at exhaustion; (3) 15 minutes after exercise; and (4) 30 minutes after exercise. Two-way ANOVA was used for statistical analysis. Results: In the first exercise test, at exhaustion and 15 minutes after exercise, SOD activity in red blood cells of those who had regular exercise habits (PE and AE) were significantly higher than those who had no regular exercise habits (PN and AN). For the second exercise test, at exhaustion, SOD activity of PE and AE were both significantly higher than that of PN and AN. Between the two exercise tests, performances and SOD activity showed no significant differences in each group. At exhaustion of the first exercise test, AE and AN showed no significant differences (p= .079). However, in the second exercise test, there were significant differences (p= .002) and the peak value of SOD activity of AE appeared at exhaustion, instead of 15 minutes after exercise. At exhaustion, SOD activity in the first exercise test was significantly higher than that in the second test (p= .035). Conclusions: For those who had regular exercise habits, two-week ALA supplementation had no additive effects, but it effectively increases SOD activity at exhaustion to counteract with oxidative stress. For those who had no regular exercise habits, SOD does not decrease significantly. This research reassures that regular exercise can effectively increase SOD activity and antioxidant capacity in human. The suggestion is that while taking antioxidant, regular exercise habit is necessary to increase human antioxidant capacity and increases SOD activity effectively to decrease damage from oxidative stress.

    第壹章 緒 論 1 一、問題背景 1 二、研究目的 3 三、名詞操作性定義 3 四、研究假設 4 五、研究範圍與限制 4 六、研究的重要性 5 第貳章 相關文獻探討 6 ㄧ、自由基及活性氧物種 6 二、運動與氧化壓力 7 三、抗氧化酶與抗氧化劑 9 四、硫辛酸的應用 12 五、本章總結 16 第參章 研究方法與步驟 18 一、研究對象 18 二、研究設計 18 三、實驗時間與地點 18 四、實驗方法與步驟 19 五、實驗操作流程 20 六、血液生化分析 21 七、統計分析 21 第肆章 結 果 22 一、受試者基本生理資料 22 二、第一次運動測試SOD活性分析 23 三、第二次運動測試SOD活性分析 26 四、兩次運動測試運動表現及SOD活性比較 28 第伍章 討論與結論 30 一、規律運動習慣對SOD活性的影響 30 二、衰竭性運動對體內SOD活性變化的影響 31 三、抗氧化劑硫辛酸介入對SOD活性的的影響 32 四、結論 35 引用文獻 36 附錄一 運動習慣調查表 41 附錄二 受試者告知同意書 42 附錄三 受試者健康情況調查表 44 附錄四 本研究使用之硫辛酸 45 附錄五 bruce Potocal衰竭運動測試流程 46

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