簡易檢索 / 詳目顯示

研究生: 呂至堅
Chih-Chien Lu
論文名稱: 寬尾鳳蝶屬之親緣關係及保育遺傳研究
Molecular phylogeny and conservation genetics of Agehana butterflies(Lepidoptera : Papilionidea)
指導教授: 徐堉峰
學位類別: 博士
Doctor
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2008
畢業學年度: 96
語文別: 中文
論文頁數: 110
中文關鍵詞: 寬尾鳳蝶COI序列COII序列粒線體基因體親緣關係保育
論文種類: 學術論文
相關次數: 點閱:147下載:20
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 寬尾鳳蝶屬(Agehana)的蝴蝶後翅具有不尋常的寬闊尾突,而且其內有兩條翅脈穿過,由於形態特徵與其他蝴蝶明顯不同,因此其分類歸群、親緣關係等尚未有明確定論,值得深入探討與釐清。目前一般認為這個屬有兩個物種,而台灣寬尾鳳蝶(A. maraho)被認為是台灣一個特有且瀕危的分類單元;另外一個物種是廣泛分佈於中國大陸南方的中華寬尾鳳蝶(A. elwesi)。本研究針對寬尾鳳蝶之屬級親緣關係及遺傳保育,分別進行三個部分的研究探討。
    中華寬尾鳳蝶有一型的個體外表相似於台灣寬尾鳳蝶,被稱為中華寬尾鳳蝶白斑型,分佈地侷限於中國大陸西南地區一帶。此一白斑型與台灣寬尾鳳蝶以往並沒有從形態或分子方面作嚴格的檢視。第一部分的探討即針對雄性交尾器及CO I基因1531個鹼基對片段所做的基因序列檢測,結果顯示白斑型個體較近似於中華寬尾鳳蝶黑色型,與台灣寬尾鳳蝶關係較遠。此一結果支持白斑型個體僅是中華寬尾鳳蝶族群內之一「型」,而非亞種。
    第二部份針對寬尾鳳蝶的屬級親緣關係進行探討,結果顯示寬尾鳳蝶屬的兩個分類單元彼此的分化時間極短且關係非常近緣,而與北美地區的Pterourus屬的鳳蝶結合成單系群。此一結果產生了一個極戲劇性的討論空間。本研究的初步建議暫時將寬尾鳳蝶屬處理成廣義鳳蝶屬 (Papilio sensu lato)的亞屬,或是併入Pterourus屬內。
    先前以粒線體COI及COII序列分析,指出中華寬尾鳳蝶與台灣寬尾鳳蝶為兩個獨立種,但獨立種的分析差異小於0.5%。故欲利用完整粒線體DNA的定序分析,以了解寬尾鳳蝶兩獨立種間的分化時間。並以粒線體不同片段的序列分析,提供其它鱗翅目選用序列時的參考。第三部分的研究成功定出寬尾鳳蝶完整的粒線體基因體序列分別為 16093 bp(A. elwesi elwesi)、16095 bp(A. elwesi caveri) 及 16094 bp (A. maraho)。三個寬尾鳳蝶粒線體基因體的基因組成及排列方式與大部分的鱗翅目相同,僅在控制區(Control Region)與其它鱗翅目不同,具有大片段的重覆區(143bp及89bp)。三個基因體最大的變異區為cytb較一般常用的COI + COII基因的變異量多。整體的基因體變異點支持中華寬尾鳳蝶白斑型與中華寬尾鳳蝶黑色型的親緣關係較台灣寬尾鳳蝶為近。

    圖 次 ------------------------------------------------------------------------------2 表 次 ------------------------------------------------------------------------------3 中文摘要 -------------------------------------------------------------------------4 Abstract ----------------------------------------------------------------------------6 前 言 ------------------------------------------------------------------------------8 Part I.:由形態及分子證據來探討中華寬尾鳳蝶白斑型之分類位階 --------------------------------------------------------------------------------------12 Part II.:寬尾鳳蝶屬級親緣關係之研究 ----------------------------------21 Part III.:寬尾鳳蝶屬粒線體基因體序列變異比較 ----------------------31 誌 謝 -----------------------------------------------------------------------------35 參考文獻 ------------------------------------------------------------------------36 論文內容相關「圖、表」 ---------------------------------------------------45 附錄一、研究之相關照片 ----------------------------------------------------67 附錄二、已發表或已接受之文章 --------------------------------------------71

    山中正夫。1971。台灣產蝶類的の分佈(1)。日本鱗翅學會特別報告。5:115-191。
    大野義昭。1980。 フトオアゲハについて。やどりが。101-102:27-33。
    五十嵐邁。1979。世界のアゲハチョウ。講談社。
    白水 隆。1960。原色台灣蝶類大圖鑑。保育社。
    白九維、王效岳。1998。台灣的鳳蝶與中國大陸種類的綜述。淑馨出版社。
    呂勝由。1996。台灣稀有及瀕危植物之分級彩色圖鑑(I)。行政院農業委員會。
    李傳隆、張立軍。1984。中國產珍蝶寬尾鳳蝶的校定。動物分類學報9(3):335。
    林春吉。1994。台灣的蝴蝶與自然之美。三隻小豬國際有限公司。
    周 堯。1994。中國蝶類志。河南科學技術出版社。
    周 堯。1999。中國蝴蝶原色圖鑑。河南科學技術出版社。
    周麗君、張立軍。1981。寬尾鳳蝶白斑亞種的初步觀察。昆蟲知識18(6):254-255。
    陳維壽。1974。台灣區蝶類大圖鑑。中國文化雜誌社。
    陳漢林、黃水生。1993。中華寬尾鳳蝶的生物學特性。浙江農業大學學報19(2):128。
    素木得一、楚南仁博。1934。新に発見られたゐフトオアゲハに就いて。Zephyrus 5(4): 177-182.pl.15
    徐堉峰。2006。台灣蝶圖鑑第三卷。國立鳳凰谷鳥園。
    徐堉峰、王立豪、呂至堅、吳立偉、黃嘉龍。2006a。寬尾鳳蝶的生態習性與生態需求之研究(二)。行政院農業委員會林務局新竹林區管理處。
    徐堉峰、陳建志、楊平世。1986。台灣特產種蝶類之綜述。國立台灣大學農學院研究報告26(1):55-69。
    張潮巨、盧福生、陳順立、李友恭、陳志平。1991。中國寬尾鳳蝶的研究。福建林學院學報11(1):59-66。
    黃邦侃、齊石成、吳小玲。1993。海峽兩岸的寬尾鳳蝶。華東昆蟲學報2(1):5-9。
    楊平世。1992。寬尾鳳蝶之分佈現況及其生態研究。台灣省農林廳林務局。
    楊平世、李志穎。1997。稀有種生物之生態調查—寬尾鳳蝶之生態研究(二)。內政部營建署雪壩國家公園管理處。
    楊義賢。1967。フトオアゲハの生活史について。蝶と蛾18:44-45。
    楊耀隆。1996。保育類野生動物圖鑑-昆蟲類。台灣省特有生物研究保育中心。
    詹家龍、羅錦文。2000。寬尾鳳蝶之復育研究。內政部營建署雪壩國家公園管理處。
    廖有麟。1967。フトオアゲハ幼蟲の發見。蝶と蛾18:42-43。
    廖有麟。1969。台灣產フオトアゲハの幼虫と蛹。蝶と蛾20: 85-88。
    廖有麟、張士珍。1986。國蝶-寬尾鳳蝶生態研究。中華民國第25屆中小學科學展覽會。
    鄭錫奇、姚正得、林華慶、李德旺、林麗紅、盧堅富、楊燿隆、賴景陽。1996。保育類野生動物圖鑑。特有生物研究保育中心。
    濱野榮次。1987。台灣蝶類生態大圖鑑。牛頓出版社。
    顏聖紘、楊平世。2000。保育類昆蟲(附CITES附錄物種)鑑識參考圖冊。行政院農業委員會。
    Aubert, J., L. Legal, H. Descimon, and F. Michel. 1999. Molecular phylogeny of swallowtail butterflies of the tribe Papilionini (Papilionidae, Lepidoptera). Mol Phylogenet Evol 12:156-167.
    Boore, J. L. 1999. Animal mitochondrial genomes. Nucleic Acids Res 27:1767-1780.
    Boore, J. L., J. R. Macey, and M. Medina. 2005. Sequencing and comparing whole mitochondrial genomes of animals. Methods Enzymol 395:311-348.
    Bremer, K. 1998. The limits of amino acid sequence data in angiosperm phylogenetic reconstruction. Evolution 42:795-803.
    Brower LP. 1984. Chemical defence in butterflies. In Van-Wright, RI, PR Ackery: The Biology of Butterflies. Pp. 109-134. London: Academic Press.
    Brower, A. V.Z. 1994. Phylogeny of Heliconius butterflies inferred from mitochondrial DNA sequences (Lepidoptera: Nymphalidae). Mol. Phylogenet. Evol. 3:159-174.
    Brown JW, S Miller, M Horak. 2003. Studies on New Guinea moth. 2. Description of a new species of Xenothictis Meyrick (Lepidoptera: Tortricidae: Archipini). Proc. Entomol. Soc. Wash. 105: 1043–1050.
    Cameron, S. L., K. B. Miller, C. D'Haese, M. F. Whiting, and S. C. Barker. 2004. Mitochondrial genome data alone are not enough to unambiguously resolve the relationships of Entognatha, Insecta and Crustacea sensu lato (Arthropoda). Cladistics 20:534-557.
    Caterino, M. S., R. D. Reed, M. M. Kuo, and F. A. H. Sperling. 2001. A partitioned likelihood analysis of swallowtail butterfly phylogeny (Lepidoptera: Papilionidea). Syst. Biol. 50:106-127.
    Caterino, M. S., and F. A. H. Sperling. 1999. Papilio phylogeny based on mitochondrial cytochrome oxidase I and II genes. Mol. Phylogenet. Evol. 11:122-137.
    Caterino, M. S., S. Cho, and F. A. H. Sperling. 2000. The current state of insect molecular systematics: a thriving tower of babel. Annual review of entomology. 45:1-54.
    Clary, D. O., and D. R. Wolstenholme. 1985. The mitochondrial DNA molecule of Drosophila yakuba: Nucleotide sequence, gene organization, and genetic code. Journal of Molecular Evolution 22: 252-271.
    Clement M, D Posada, K Crandall. 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology 9: 1657-1660.
    Coates, B. S., D. V. Sumerford, R. L. Hellmich, and L. C. Lewis. 2005. Partial mitochondrial genome sequences of Ostrinia nubilalis and Ostrinia furnicalis. Int J Biol Sci 1:13-18.
    Collins NM, MG Morris, 1985. Threatened Swallowtail Butterflies of the World: The IUCN Red Data Book.
    Costa FO, JR deWaard, JB Boutillier, S Ratnasingham, R Dooh, M Hajibabaei, PD Hebert. 2007. Biological identification through DNA barcodes: the case of the Crustacea. Can. J. Fish. Aquat. Sci. 64: 272-295.
    D’Abrera, B. 1982. Butterflies of the Oriental Region, Part I. Victoria: Hill House.
    DeSalle, R., T. Freedman, E. M. Prager, and A. C. Wilson. 1987. Tempo and mode of sequence evolution in mitochondrial DNA of Hawaiian Drosophila. J.Mol. Evol. 26:157-164.
    Eliot JN. 1992. The butterflies of the Malay Peninsula. 4th revised ed. (originally by AS Corbet and HM Pendlebury,). Kuala Lumpur: The Malayan Nature Society.
    Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 39:783-791.
    Gimenz Dixon, M. 1996. The IUCN/SSC Action Plans for species conservation, concepts and basic guidelines. In: Proceedings of the Anatidae 2000 Conferences, Strasbourg, France, 5-9December 1994, M. Birkan, J. van Vessem, P. Havet, J. Madsen, B. Trolliet. & M. Moser eds. Gibier Faune Sauvage, Game and Wildlife13: 1143-1150.
    Guindon, S. and O. Gascuel. 2003. A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood. Systematic Biology 52: 696-704.
    Hajibabaei, M., M. A. Smith, D. H. Janzen, J. J. Rodriguez, J. B. Whitfield, and P. D. N. Ebert. 2006. A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes 6(4): 959-964.
    Hancock, D. L. 1983. Classification of the Papilionidea(Lepidoptera): A phylogenetic approach. Smithersia 2:1-48.
    Häuser, C. L., R. de Jong, G. Lamas, R. K. Robbins, C. Smith, and R. I. Vane-Wright. 2002. Papilionidae—Revised GloBIS/GART species checklist, 2nd draft. Available at http://www.insectsonline. de/frames/papilio.htm.
    Hebert, P. D. N., E. H. Penton, J. M. Burns, D. H. Janzen, and W. Hallwachs. 2004. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator Proccdings of the National Academy of Sciences USA 101:14812-14817.
    Hemming, F. 1939. Notes on the generic nomenclature of the Lepidoptera Rhopalocera. I. Proc. R. ent. Soc. Lond. (B) 8: 133-138.
    Huelsenbeck, J. P.,and F. Ronquist. 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754-755.
    Igarashi, S. 1984. The classification of the Papilionidae mainly based on the morphology of their immature stages. Tyo to Ga 34:41-96.
    Igarashi, S. Papilionidae and Their Early Stages. Tokyo: Kodansha. [In Japanese]
    IUCN, 2006. 2006 IUCN Red list of threatened species. Available from http://www.iucnredlist.org/search/details.php/15998/summ
    Kim, I., E. M. Lee, K. Y. Seol, E. Y. Yun, Y. B. Lee, J. S. Hwang, and B. R. Jin. 2006. The mitochondrial genome of the Korean hairstreak, Coreana raphaelis (Lepidoptera: Lycaenidae). Insect Mol. Biol. 15:217-225.
    Klots AB. 1970. Lepidoptera. In SL Tuxen, ed. Taxonomist’s glossary of genitalia in insects, 2nd edn. Copenhagen: Munksgaard, pp. 115-130.
    Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150-163.
    Le Cerf, M. F. 1923. Descriptions de formes nouvelles de Lépidoptères Rhopalocères. Bulletin du Muséum histoire Naturella 24: 360 – 367.
    Lee, E. S., K. S. Shin, M. S. Kim, H. Park, S. Cho, and C. B. Kim. 2006. The mitochondrial genome of the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae). Gene 373:52-57.
    Lowe, T. M., and S. R. Eddy. 1997. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25:955-964.
    Moritz, C., T. E. Dowling, and W. M. Brown. 1987. Evolution of animal mitochondrial DNA: Relevance for Population biology and systematics. Annual Review of Ecology and Systematics 18:269-292.
    Matsumura, S. 1936. A new genus of Papilionidae. Insecta Matsumurana 10(3): 86.pl.2
    Muñoz, J. E. T. & V. R. Corrochano. 2000. Identification Guide to Butterflies Pcotected by the CITES Convention and European Union. Melbourne & London: Hill House.
    Munroe, E. 1961. The classification of the Papilionidea (Lepidoptera). Can. Entomol. Suppl. 17:1-51.
    Nazari V., E. V. Zakharov and F. A. H. Sperling. 2007. Phylogeny, historical biogeography, and taxonomic ranking of Parnassiinae (Lepidoptera, Papilionidae) based on morphology and seven genes. Molecular Phylogenetics and Evolution. 42(1): 131-156.
    Posada, D., and K. A. Crandall. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics 14:817-818.
    Powell JA, JA De Benedictis. 1995. Biological relationships:host tree preferences and isolation by pheromones among allopatric and sympatric populations of western Choristoneura. Univ. Calif. Publ. Entomol. 115: 21-68.
    Reed, R. D., and F. A. H. Sperling. 1999. Interaction of process partitions in phylogenetic analysis: An example from the swallowtail butterfly genus Papilio. Mol. Biol. Evol. 16:286–297.
    Rodriguez, F., Oliver, J.L., Marin, A., Medina, J.R., 1990. The general stochastic model of nucleotide substitution. J. Theor. Biol. 142, 485–501.
    Roehrdanz, R. L. 1995. Amplification of complete insect mitochondrial genome in two easy pieces. Insect Mol Biol 4:169-172.
    Rozas, J., J. C. Sanchez-DelBarrio, X. Messeguer, and R. Rozas. 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19:2496-2497.
    Scoble, M. J. 1992. The Lepidoptera, Form, Function and Diversity. Oxford University Press.
    Scott, J. A. 1986. The Butterflies of North America. Stanford University Press.
    Shao, R., and S. C. Barker. 2003. The highly rearranged mitochondrial genome of the plague thrips, Thrips imaginis (Insecta: Thysanoptera): convergence of two novel gene boundaries and an extraordinary arrangement of rRNA genes. Mol Biol Evol 20:362-370.
    Shao, R., N. J. Campbell, and S. C. Barker. 2001. Numerous gene rearrangements in the mitochondrial genome of the wallaby louse, Heterodoxus macropus (Phthiraptera). Mol Biol Evol 18:858-865.
    Simmons, R. B., and S. J. Weller. 2001. Utility and evolution of cytochrome b in insects. Mol Phylogenet Evol 20:196-210.
    Sillen-Tullberg B. 1988. Evolution of gregariousness in aposematic butterfly larvae: a phylogenetic analysis. Evolution 42: 293-305.
    Silva-Brandão, K. L., A. V. L. Freitas, A. V.Z. Brower and V. N. Solferini. 2005. Phylogenetic relationships of the New World Troidini swallowtails (Lepidoptera: Papilionidae) based on COI, COII, and EF-1 genes. Molecular Phylogenetics and Evolution 36: 468-483.
    Sorenson, M.D., 1999. TreeRot, Version. 2. Boston University, Boston MA. Available from http://mightyduck.bu.edu/TreeRot.
    Swofford, D. L. 1998. PUPA*: Phylogenetic analysis using parsimony (*and other methods), version 4.0. Sinauer, Sunderland, Massachusetts.
    Templeton AR, KA Crandall, CF Sing. 1992. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132: 619-633.
    Tennet, J. W. 2002. Butterflies of the Solomon Islands, Systematics and Biogeography. Dereham: Storm Entomological Publications.
    van Velzen R, F Bakker, JJA van Loon. 2007. DNA barcoding reveals hidden species diversity in Cymothoe (Nymphalidae). Proc. Neth. Entomol. Soc. Meet. 18: 95-103.
    Vila, M., and M. Björklund. 2004. The utility of the neglected mitochondrial control region for evolutionary studies in Lepidoptera (Insecta). Journal of Molecular Evolution 58:280-290.
    Wolstenholme, D. R. 1992. Animal mitochondrial DNA: structure and evolution. International Review of Cytology 141:173-216.
    Yagi, T., G. Sasaki, and H. Takebe. 1999. Phylogeny of Japanese papilionid butterflies inferred from nucleotide sequences of the mitochondrial ND5 gene. J Mol Evol 48:42-48.
    Yang, Z. 1994. Maximum likelihood phylogenetic estimation from DNA sequences with variable rates over sites: Approximate methods. Journal of Molecular Evolution 39: 306-314.
    Yen, S. H. & P. S. Yang. 2001. Illustrated Identification Guide to Insects Protected by the CITES and Wildlife Conservation Law of Taiwan, R. O. C. Council of Agriculture, Executive Yuan,Taiwan, R. O. C.
    Yukuhiro, K., H. Sezutsu, M. Itoh, K. Shimizu, and Y. Banno. 2002. Significant levels of sequence divergence and gene rearrangements have occurred between the mitochondrial genomes of the wild mulberry silkmoth, Bombyx mandarina, and its close relative, the domesticated silkmoth, Bombyx mori. Mol Biol Evol 19:1385-1389.
    Zakharov, E., M. Caterino, and F. A. H. Sperling. 2004. Molecular Phylogeny, Historical Biogeography, and Divergence Time Estimates for Swallowtail Butterflies of the Genus Papilio (Lepidoptera: Papilionidae). Systematic Biology 53(2): 193-215.

    下載圖示
    QR CODE