研究生: |
林立心 LiHsin Lin |
---|---|
論文名稱: |
碳六十固定化酵素電化學葡萄糖感測器研製與應用 Preparation and Application of Immobilized Fullerence-Enzyme Based electrochemical glucose Sensor |
指導教授: |
施正雄
Shih, Jeng-Shong |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2002 |
畢業學年度: | 90 |
語文別: | 中文 |
論文頁數: | 115 |
中文關鍵詞: | 葡萄糖 、碳六十固定化酵素 |
英文關鍵詞: | glucose, Immobilized Fullerence-Enzyme |
論文種類: | 學術論文 |
相關次數: | 點閱:210 下載:1 |
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本研究利用具混價性質之鈷赤血鹽和碳六十固定化酵素致被化學修飾電極來發展葡萄糖感測器,本實驗在0.1MKCl,pH6之磷酸鹽緩衝液中,施加電位0.0V(相對於Ag/AgCl參比電極)偵測葡萄糖,藉由含鈷赤血鹽對H2O2之催化能力,可將偵測電位降低至0.0V,且在還原模式下進行,此電位有效防止血液中電活性物質,如維生素C(Ascorbic acid),尿酸(Uric acid),乙醯基酚(Acetaminophen),半胱銨酸(Cysteine),酪銨酸(Tyrosine),半乳糖(Galactose)之干擾.H2O2氧化還原態的鈷赤血鹽而自身被還原,此還原電流和溶液中的葡萄糖濃度成正比關係.此碳六十固定化酵素感測器對葡萄糖具有不錯之線性範圍(8mM),快速的反應時間(5秒),以及不錯之靈敏度,其偵測極限可達1.6×10-6M,在20次連續操作下相對標準偏差維4.26%,使用100天後,訊號仍維持最初之80%,實驗顯示碳六十固定化酵素確實能增加訊號和延長感測器壽命.本研究也對此葡萄糖感測器探討了溫度,酸鹼度等效應.
A mixed-valence cluster of cobalt(Ⅱ)hexacyanoferrate and fullerene-enzyme based electrochemical glucose sensor was developed.A water insoluble fullerene-glucose oxidase(C60-GOD)was synthesized and applied as an immobilized enzyme on a glassy carbon electrode with cobalt(Ⅱ)hexacyanoferrate for analysis of glucose.The glucose was measured in 0.1MKCl/phosphate buffer solution with a pH of 6.0 When the electrode potential was applied at 0.0mV(v.s Ag/AgCl reference electrode).The glucose sensor exhobited efficient electro-catalytic activity toward the liberated hydrogen peroxide and allowed cathodic detection of the glucose,with no interferences from easily oxidizable constitutes such as uric acid,ascorbic acid,cysteine,tyrosine,acetaminophen and glactose.The H2O2 oxidized the reduced cobalt(Ⅱ)hexacyanoferrate and was reduced itself,and the reduced current was proportional to the concentration of glucose in aqueous solutions.The immobilized C60-GOD based glucose sensor showed a good linear response up to 8mM and a quite short response time of 5 sec.The glucose sensor also exhibited good sensitivity with a detection limit of 1.6×10-6M and a high reproducibility with a relative standard deviation(RSD) of 4.26%.The signal decreased to 80% of the initial value after continuous operations for 100 days.The immobilized Fullerene/glucose oxidase obiously enhanced the response singal and extends the lifetime of the glucose enzyme sensor.Effects of pH and temperature on the responses of the immobilized C60-GOD based glucose sensor were also studied and discussed.
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