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研究生: 羅煜凱
Lo, Yuh Kai
論文名稱: 基於主動全像激發與探測之數位全像斷層切面多模式造影
Sectional multimodality imaging in digital holographic tomography based on activated holographic pump and probe approach
指導教授: 鄭超仁
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2020
畢業學年度: 108
語文別: 中文
論文頁數: 43
中文關鍵詞: 數位全像顯微術數位全像斷層術光束掃描螢光顯微鏡電腦全像術
英文關鍵詞: digital holographic microscopy, digital holographic tomographic, beam rotation, fluorescence microscopy, computer generated hologram
DOI URL: http://doi.org/10.6345/NTNU202000531
論文種類: 學術論文
相關次數: 點閱:154下載:0
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  • 本論文為研究基於數位全像顯微術(digital holographic microscopy)和全像激發系統的(holographic pumping system)的量測方法為生物醫學樣品提供一種新穎的量測技術-數位全像斷層多模式切面造影(Sectional multimodality digital holographic tomography, SM-DHT)技術,該技術可以量測及分析樣品內的折射率分布和樣品切面的螢光影像。
    其中基於對數位全像系統(DHM system)及螢光成像系統之三維解析度分析,以及量化的將兩者三維座標軸連結,我們提出了完整連結兩系統的方法,並根據此方法設計出可主動的根據樣品的三維位置進行螢光掃描成像的系統。
    最後透過生物樣品進行實際量測,這是第一次量測及驗證人類白血病單核細胞株(THP-1)在流體介質中的折射率和螢光切面影像的關聯。

    This study presents a novel sectional multimode tomographic imaging technique for biomedical samples using an activated holographic pump and probe scheme based on digital holographic microscopy, in which the sectional image of refractive index distribution and fluorescence inside the sample can be measured and analyzed. To accomplish the activated holographic pump and probe, the target-linking between object plane and pumping plane has been analyzed based on the coordinate transfer from digital holographic reconstruction to pumping points designing in computer generated holograms. The resolution of the pump and probe system also theoretically discussed to achieve the precise pumping at specific object plane. The sectional image of excitation fluorescence information from each pumping point can thus be obtained to analyze with the tomographic image from holographic imaging system. It is the first time that the sectional image of refractive index fluorescence of Human leukemia monocytic THP-1 in a fluid medium is measured and verified.

    致謝 I 論文摘要 II ABSTRACT III 目錄 IV 圖目錄 VI 第一章 緒論 1 1.1 技術背景 1 1.2 研究動機 6 1.3 論文架構 7 第二章 主動式的全像探測與激發 9 2.1 多層數位全像顯微系統重建解析度 9 2.2 主動式全像激發系統設計及解析度分析 12 2.2.1 全像主動式激發系統設計 12 2.2.2 全像主動式激發系統解析度分析 15 2.3 數位全像三維重建應用於三維螢光定位 16 2.3.1 目標鏈接(Target-linking) 16 2.3.2 實驗流程與結果 19 第三章 細胞構造切面資訊量測 23 3.1 數位全像斷層顯微術 23 3.1.1 合成孔徑數位全像術 23 3.1.2 光束掃描式數位全像斷層顯微術 24 3.2 掃描式螢光成像 26 第四章 基於主動式全像激發與探測之多切面成像技術 30 4.1 實驗原理與架構 30 4.1.1 實驗原理及流程 30 4.1.2 實驗架構 32 4.2 實驗結果與討論 33 4.3 附註:實驗樣品製備 36 第五章 結論及未來展望 38 參考文獻 39 附錄A 發表論文 43

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