簡易檢索 / 詳目顯示

研究生: 羅煜凱
Lo, Yuh Kai
論文名稱: 基於主動全像激發與探測之數位全像斷層切面多模式造影
Sectional multimodality imaging in digital holographic tomography based on activated holographic pump and probe approach
指導教授: 鄭超仁
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2020
畢業學年度: 108
語文別: 中文
論文頁數: 43
中文關鍵詞: 數位全像顯微術數位全像斷層術光束掃描螢光顯微鏡電腦全像術
英文關鍵詞: digital holographic microscopy, digital holographic tomographic, beam rotation, fluorescence microscopy, computer generated hologram
DOI URL: http://doi.org/10.6345/NTNU202000531
論文種類: 學術論文
相關次數: 點閱:122下載:0
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 本論文為研究基於數位全像顯微術(digital holographic microscopy)和全像激發系統的(holographic pumping system)的量測方法為生物醫學樣品提供一種新穎的量測技術-數位全像斷層多模式切面造影(Sectional multimodality digital holographic tomography, SM-DHT)技術,該技術可以量測及分析樣品內的折射率分布和樣品切面的螢光影像。
    其中基於對數位全像系統(DHM system)及螢光成像系統之三維解析度分析,以及量化的將兩者三維座標軸連結,我們提出了完整連結兩系統的方法,並根據此方法設計出可主動的根據樣品的三維位置進行螢光掃描成像的系統。
    最後透過生物樣品進行實際量測,這是第一次量測及驗證人類白血病單核細胞株(THP-1)在流體介質中的折射率和螢光切面影像的關聯。

    This study presents a novel sectional multimode tomographic imaging technique for biomedical samples using an activated holographic pump and probe scheme based on digital holographic microscopy, in which the sectional image of refractive index distribution and fluorescence inside the sample can be measured and analyzed. To accomplish the activated holographic pump and probe, the target-linking between object plane and pumping plane has been analyzed based on the coordinate transfer from digital holographic reconstruction to pumping points designing in computer generated holograms. The resolution of the pump and probe system also theoretically discussed to achieve the precise pumping at specific object plane. The sectional image of excitation fluorescence information from each pumping point can thus be obtained to analyze with the tomographic image from holographic imaging system. It is the first time that the sectional image of refractive index fluorescence of Human leukemia monocytic THP-1 in a fluid medium is measured and verified.

    致謝 I 論文摘要 II ABSTRACT III 目錄 IV 圖目錄 VI 第一章 緒論 1 1.1 技術背景 1 1.2 研究動機 6 1.3 論文架構 7 第二章 主動式的全像探測與激發 9 2.1 多層數位全像顯微系統重建解析度 9 2.2 主動式全像激發系統設計及解析度分析 12 2.2.1 全像主動式激發系統設計 12 2.2.2 全像主動式激發系統解析度分析 15 2.3 數位全像三維重建應用於三維螢光定位 16 2.3.1 目標鏈接(Target-linking) 16 2.3.2 實驗流程與結果 19 第三章 細胞構造切面資訊量測 23 3.1 數位全像斷層顯微術 23 3.1.1 合成孔徑數位全像術 23 3.1.2 光束掃描式數位全像斷層顯微術 24 3.2 掃描式螢光成像 26 第四章 基於主動式全像激發與探測之多切面成像技術 30 4.1 實驗原理與架構 30 4.1.1 實驗原理及流程 30 4.1.2 實驗架構 32 4.2 實驗結果與討論 33 4.3 附註:實驗樣品製備 36 第五章 結論及未來展望 38 參考文獻 39 附錄A 發表論文 43

    [1] David J. Stephens1, Victoria J. Allan, “Light Microscopy Techniques for Live Cell Imaging,” Science 300, 5616, 82-86 (2003)
    [2] Melanie M. Frigault, Judith Lacoste, Jody L. Swift, Claire M. Brown, “ Live-cell microscopy – tips and tools,” J. Cell SCI. 122, 753-767 (2009)
    [3] Rikke Louise Meyer, Xingfei Zhou, Ayyoob Arpanaei, Peter Kingshott, Flemming Besenbacher, “Immobilisation of living bacteria for AFM imaging under physiological conditions,” Ultramicroscopy 10, 11, 1349-1357 (2010)
    [4] Lichtman JW, Conchello JA, “Fluorescence microscopy,” Nat. Methodsvol 2, 910-919 (2005)
    [5] Dmitry A. Nedosekin, Mustafa Sarimollaoglu,Ekaterina I. Galanzha, Rupa Sawant, Vladimir P. Torchilin, Vladislav V. Verkhusha, Jie Ma, Markus H. Frank, Alexandru S. Biris and Vladimir P. Zharov, “Synergy of photoacoustic and fluorescence flow cytometry of circulating cells with negative and positive contrasts,” J. Biophotonics 6, 5, 425–434 (2013)
    [6] Jessica A. Carr, Daniel Franke, Justin R. Caram, Collin F. Perkinson, Mari Saif, Vasileios Askoxylakis, Meenal Datta, Dai Fukumura, Rakesh K. Jain, Moungi G. Bawendi, and Oliver T. Bruns, “Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green,” Proc. Natl. Acad. Sci. U.S.A 115, 4465-4470 (2018)
    [7] Ichirou Yamaguchi, Jun-ichi Kato, Sohgo Ohta, and Jun Mizuno, “Image formation in phase-shifting digital holography and applications to microscopy,” Appl. Opt. 40, 34, 6177-6186 (2001)
    [8] Pierre Marquet, Benjamin Rappaz, Pierre J. Magistretti, Etienne Cuche, Yves Emery, Tristan Colomb, and Christian Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30, 5, 468-470 (2005)
    [9] Björn Kemper and Gert von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47, 4, A52-A61 (2008)
    [10] Nicolas Pavillon , Jonas Kühn, Corinne Moratal, Pascal Jourdain, Christian epeursinge, Pierre J. Magistretti , Pierre Marquet, “Early Cell Death Detection with Digital Holographic Microscopy,” PLOS ONE 7, 1, e30912 (2102)
    [11] P. Y. Liu, L. K. Chin, W. Ser, H. F. Chen, C.-M. Hsieh, C.-H. Lee, K.-B. Sung, T. C. Ayi, P. H. Yap, B. Liedberg, K. Wang, T. Bourouina and Y. Leprince-Wang, “Cell refractive index for cell biology and disease diagnosis: past, present and future,” Lab Chip 16, 634-644 (2016)
    [12] Chung-Hsin Wu, Xin-Ji Lai, Chau-Jern Cheng, Yu-Chen Yu, and Chun-Yen Chang, “Applications of digital holographic microscopy in therapeutic evaluation of Chinese herbal medicines,” Appl. Opt. 53, 27, 192-197 (2014)
    [13] Vishesh Dubey, Azeem Ahmad, Rajwinder Singh, Deanna L Wolfson, Purusotam Basnet, Ganesh Acharya, Dalip Singh Mehta, and Balpreet Singh Ahluwalia, “Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages,” Opt. Exp. 26, 16, 19864-19876 (2018)
    [14] Xiangyu Quan, Osamu Matoba, and Yasuhiro Awatsuji, “Image recovery from defocused 2D fluorescent images in multimodal digital holographic microscopy,” Opt. Lett. 42, 9, 1796-1799 (2017)
    [15] YongKeun Park, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, “Diffraction phase and fluorescence microscopy,” Opt. Exp. 14, 18, 8263-8268 (2006)
    [16] Xiangyu Quan, Kouichi Nitta, Osamu Matoba, Peng Xia, Yasuhiro Awatsuji, “Phase and fluorescence imaging by combination of digital holographic microscopy and fluorescence microscopy,” Opt. Rev. 22, 2, 349-353 (2015)
    [17] Bradley W. Schilling, Ting-Chung Poon, Guy Indebetouw, Brian Storrie, K. Shinoda, Y. Suzuki, and Ming Hsien Wu, “Three-dimensional holographic fluorescence microscopy,” Opt. Lett. 22, 19, 1506-1508 (1997)
    [18] Joseph Rosen, Gary Brooker, “Non-scanning motionless fluorescence three-dimensional holographic microscopy,” Nat. Photonics 2, 190-195 (2008)
    [19] Ryosuke Abe, Yoshio Hayasaki, “Holographic fluorescence mapping using space-division matching method,” Opt. Commun. 401, 15, 35-39 (2017)
    [20] V. Lauer, “New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope,” J. Microscopy 205, 2, 165-176 (2002)
    [21] Witold Gorski and Wolfgang Osten, “Tomographic imaging of photonic crystal fibers,” Opt. Lett. 32, 14, 1977-1979 (2007)
    [22] Bertrand Simon, Matthieu Debailleul, Mounir Houkal, Carole Ecoffet, Jonathan Bailleul, Joël Lambert, Arnaud Spangenberg, Hui Liu, Olivier Soppera, and Olivier Haeberlé, “Tomographic diffractive microscopy with isotropic resolution,” Optica 4, 4, 460-463 (2017)
    [23] Yu-chih Lin, Hui-Chi Chen, Han-Yen Tu, Chin-Yu Liu, and Chau-Jern Cheng, “Optically driven full-angle sample rotation for tomographic imaging in digital holographic microscopy,” Opt. Lett. 42, 7, 1321-1324 (2017)
    [24] Nicolas Pavillon, Alexander Benke, Daniel Boss, Corinne Moratal, Jonas Kühn, Pascal Jourdain, Christian Depeursinge, Pierre J. Magistretti, Pierre Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3, 7, 432-436 (2010)
    [25] Schürmann M, Cojoc G, Girardo S, Ulbricht E, Guck J, Müller P, “Three-dimensional correlative single-cell imaging utilizing fluorescence and refractive index tomography,” J. Biophotonics 11, 3, e201700145 (2018)
    [26] Kyoohyun Kim, Wei Sun Park, Sangchan Na, Sangbum Kim, Taehong Kim, Won Do Heo, and YongKeun Park, “Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging,” Biomed. Opt. Express 8, 12, 5688-5697 (2017)
    [27] Gang Pan and Hui Meng, “Digital holography of particle fields: reconstruction by use of complex amplitude,” Appl. Opt. 42, 5, 827-833 (2003)
    [28] S. Murata and N. Yasuda, “Potential of digital holography in particle measurement,” Opt. Laser Technol. 32, 7-8, 567–574 (2000)
    [29] F. Dubois, Cédric Schockaert, Natacha Callens, and Catherine Yourassowsky, "Focus plane detection criteria in digital holography microscopy by amplitude analysis," Opt. Express 14, 5895-5908 (2006)
    [30] T. WILSON, “Resolution and optical sectioning in the confocal microscope,” J. Microscopy 224, 2, 113-121 (2011)
    [31] Xin-Ji Lai, Han-Yen Tu, Chung-Hsin Wu, Yu-Chih Lin, and Chau-Jern Cheng, “Resolution enhancement of spectrum normalization in synthetic aperture digital holographic microscopy,” Appl. Opt. 54, 1, A51-A58 (2015)

    無法下載圖示 本全文未授權公開
    QR CODE