研究生: |
林國英 Kuo-Yin Lin |
---|---|
論文名稱: |
不同光質對甘藷小苗光合作用效率、碳水化合物代謝及抗氧化能力之影響 The effects of different light qualities on the photosynthesis efficiency, carbohydrate metabolism and antioxidant capacity of the sweet potato seedings |
指導教授: |
王玉麒
Wang, Yu-Chie |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
論文出版年: | 2011 |
畢業學年度: | 99 |
語文別: | 中文 |
論文頁數: | 98 |
中文關鍵詞: | 光質 、發光二極體 、甘藷小苗 、光合作用效率 、碳水化合物代謝 、抗氧化能力 |
英文關鍵詞: | light quality, light emitting diode (LED), sweet potato seeding, photosynthesis efficiency, carbohydrate metabolism, antioxidant capacity |
論文種類: | 學術論文 |
相關次數: | 點閱:290 下載:18 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
甘藷(Ipomoea batatas (L.) Lam.)葉是重要的抗氧化蔬菜,發光二極體(Light Emiting Diode, LED)則由於可改善傳統燈具壽命短、發熱大和發光效率差等缺點,並提供包括光質、光量、給光頻率及工作比等特定給光條件,成為近年來積極開發之新興人工光源。本論文利用LED提供甘藷小苗藍光(470 nm)與紅光(660 nm),除了探討抗氧化特性外,亦分析光合效率與碳水化合物的代謝受不同光質的影響情形,探討不同光質對甘藷小苗生理之影響。
透過葉綠素螢光數據,經藍光、紅光或全光5天照射處理後,皆不至於對甘藷小苗光合系統Ⅱ造成破壞。由於紅光組甘藷小苗葉片之NPQ明顯低於藍光或全光處理,根據前人指出NPQ與zeaxanthin具正相關性,推測紅光會降低甘藷葉生合成zeaxanthin。
抗氧化能力上,經藍光、紅光和全光處理後之甘藷小苗葉片在glutathion還原態(GSH)及氧化態(GSSG)的含量、ascorbate peroxidases(APX)活性和superoxide dismutase(SOD)活性上並未呈現顯著差異,但藍光處理之葉片表現較高的catalase(CAT)活性,且經藍光或紅光處理後之葉片H2O2含量均較全光低,推測藍光組可透過CAT降低活氧化物的累積。
經測定可溶性醣類含量、澱粉含量與醣類代謝相關酵素活性,顯示不同光質雖未在澱粉含量造成影響,但藍光處理之葉片內葡萄糖、果糖及蔗糖含量皆明顯高於紅光處理,推測與藍光組較紅光組有較高之sucrose phosphate synthase(SPS)和invertase(INV)活性有關。此外全光處理之葉片具較高之acid INV和alkaline INV活性,紅光處理的acid INV和alkaline INV活性則均較藍光處理為低。不同光質下醣類代謝相關酵素活性以SPS和INV表現出顯著差異,但Real-time PCR偵測各酵素基因表現強度並無差別,推測此二者活性應受後轉譯調節所影響。不同光質5天照射雖未對葉片氣孔密度造成影響,但以藍光組氣孔開啟比例最高。
總體而言,藍光處理之甘藷小苗生長良好、有較高之抗氧化能力,葉片內葡萄糖、果糖及蔗糖含量均較高,也具有較高的氣孔開啟比例,顯示在甘藷小苗生長上,藍光較全光、紅光為佳。
The leaf of sweet potato (Ipomoea batatas (L.) Lam.) is an important antioxidant vegetable. The light emitting diode (LED) is a promising artificial light source because of its small mass and volume, and can provide specific light quality, light quantity, light frequency and duty ratio. This paper uses the LED as an artificial light source to provide blue light (470 nm) and red light (660 nm) for sweet potato seedings, and discusses the antioxidant capacity, photosynthesis efficiency, and carbohydrate metabilism of the sweet potato seedings cultivated under different light qualities.
The data of chlorophyll fluorescence showed that photosystemⅡof the sweet potato seedings cultivated under blue, red or full-light for 5 days was not destroyed. NPQ of the sweet potato leaves cultivated under red light was significantly lower than that under blue or full-light. According to the previous research data, we can suggest that the leaves of sweet potato under red light might reduce the biosynthesis of zeaxanthin.
The reduced glutathion (GSH) content, oxidized glutathion (GSSG) content, ascorbate peroxidase (APX) activity and superoxide dismutase (SOD) activity of the sweet potato leaves cultivated under blue, red or full-light for 5 days didn’t show the significant difference, but catalase (CAT) content of the sweet potato leaves cultivated under blue light was higher, and H2O2 content of the sweet potato leaves cultivated under blue or red-light was lower than that under full light. We can presume that the reactive oxygen species content of the sweet potato leaves cultivated under blue light reduced by CAT.
The results of the contents of carbohydrates and the activities of the enzymes to participate in carbohydrate metabilism showed the different light qualities didn’t affect starch content of the sweet potato leaves, but glucose, fructose and sucrose contents of the sweet potato leaves cultivated under blue light were remarkably higher than those under red light. We can speculate that the activities of sucrose phosphate synthase (SPS) and invertase (INV) of the sweet potato leaves cultivated under blue light were higher than those under red light was the reason for foregoing results. Furthermore, the sweet potato leaves cultivated under full light had higher activities of acid INV and alkaline INV, and those under red light had lower activities of acid INV and alkaline INV than those under blue light. The activites of SPS and INV of the sweet potato leaves cultivated under blue, red, or full-light had significant difference, but the expressions of gene of SPS, sucrose synthase (Susy) , and INV displayed by Real-time PCR were not different. Therefore, the activity of SPS and INV might be affected by post-translation. Although the densities of the stroma of the sweet potato leaves cultivated under blue, red, or full-light didn’t have significant difference, the ratio of the open stroma of the sweet potato leaves cultivated under blue light was the highest.
As a whole, the sweet potato seedings cultivated under blue light were growing abundantly, and the leaves of those had higher antioxidant capacity, more glucose, more fructose, more sucrose, and higher ratio of the open stroma. Those results indicate that blue light is better than red or full-light for sweet potato seedings.
行政院農業委員會http://www.coa.gov.tw/show_index.php
行政院農業委員會農糧署http://www.afa.gov.tw/index.asp
王昭月、曾夢蛟(2010)利用葉綠素營光與有效授粉估測甜椒之耐熱性。臺灣農業研究59: 237–248.
方煒、饒瑞佶(2004)高亮度二極體於生物產業之應用。中華農學會報5: 432–434.
朱大鈞(2000)高滲透逆境甘藷懸浮細胞蔗糖磷酯合成酶之生化學研究。國立臺灣大學農業化學研究所。碩士論文。
李木和(2004)光照與滲透逆境下水稻蔗糖磷酯合成酶性質鑑定與之免疫組織定位。國立臺灣大學農業化學研究所。博士論文。
李良展(2008)野外實測北投溫泉紅藻在不同環境條件下之光合作用能力。國立彰化師範大學生物學系。碩士論文。
吳宗諺、利幸真、邱采新和蔡淑珍(2009)探討不同品種甘薯品種熱水萃取液汁抗氧化能力。臺灣農業研究58: 7–16.
汪澤宏(2007)即時聚合酶連鎖反應(Real-time PCR)在植物病蟲害檢測上之應用。農政與農情181: 104–106.
林安秋(1991)臺灣商務印書館股份有限公司。作物之光合作用pp.166–167。
林怡君(2002)臺農57、臺農68品種甘藷超低溫冷凍保存前處理流程之探討。國立中興大學植物學系碩士班。碩士論文。
姚銘輝、盧虎生和朱鈞(2002)葉綠素螢光與作物生理反應。科學農業50: 31–41.
高天文(2004)蝴蝶蘭中逆境相關訊息傳遞基因之選殖與特性分析。國立成功大學生命科學系碩博士班。碩士論文。
徐邦達(2002)葉綠素螢光和PAM螢光儀:原理及測量。光合作用研討會pp.1–9。
韋朝領、江昌俊和陶漢之(2005)依賴於葉黃素循環的熱耗散對茶樹葉片防禦強光破壞的相關試驗研究。激光生物學報14(3): 218–223.
陳勁中(2001)甘藷葉蔗糖磷酯合成酶之生化學研究。國立臺灣大學農業化學研究所。碩士論文。
陳韋良(2003)滲透逆境誘發甘藷塊根懸浮細胞培養中蔗糖磷酯合成酶之生化與分子生物學研究。國立臺灣大學農業化學研究所。博士論文。
陳冀嫺(2001)甘藷桃園二號對鹽分逆境之抗氧化物質的生化反應研究。國立中央大學生命科學研究所。碩士論文。
黃東健(2001)甘藷蔗糖磷酯合成酶之生化和分子生物學研究。國立臺灣大學農業化學研究所。博士論文。
楊純明、李裕娟(2009)從植物之光週期看發光二極體在農業生產上之應用潛力。作物、環境與生物資訊6: 192–200.
廖國吟(2007)三種殼斗科樹苗在水逆境處理及恢復供水對生長、光合作用及葉綠素螢光表現之影響。國立臺灣大學生物資源暨農學院森林暨資源學系。碩士論文。
劉邦熙(1996)甘藷蔗糖磷酯合成酶之生化學研究。國立臺灣大學農業化學系。碩士論文。
饒瑞佶、方煒(2003a)光質對於彩色海芋組培苗生長之影響。九十二年農業機械論文發表會。8月28–29日,臺北。中華民國。
饒瑞佶、方煒(2003b)光量與光週期對馬鈴薯組培苗生長的影響。九十二年農業機械論文發表會。8月28–29日,臺北。中華民國。
Anderson ME. (1985) Determination of glutathione and glutathione disulfide in biological samples. Meth Enzymol 113: 548–555.
Asada K. (1992) Ascorbate peroxidase – a hydrogen peroxide – scavenging enzyme in plants. Physiol Plant 85: 235–241.
Balibrea ME, Rus-Alvarez AM, Bolarin MC and Perez-Alfocea F. (1997) Fast changes in soluble carbohydrates and proline contents in tomato seedlings in response to ionic and nonionic iso-osmotic stresses. J Plant Physiol 151: 221–226.
Barnes C and Bugbee B. (1991) Morphological responses of wheat to changes in phytochrome equilibrium. Plant Physiol 97: 359–365.
Berger D and Altmann T. (2000) A subtilisin- like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana. Genes Dev 14: 1119–1131.
Bertamini M and Nedunchezhian N. (2003) Photoinhibition of photosynthesis in mature and young leaves of grapevine (Vitis vinifera L.). Plant Sci 10: 635–644.
Bowler C, Van Camp W, Van Montagu M and Inzé D. (1994) Superoxide dismutase in plants. Crit Rev Plant Sci 13: 199–218.
Bueno P, Piqueras A, Kurepa J, Savouré A, Verbruggen N, Van Montagu M and Inzé D. (1998) Expression of antioxidant enzymes in response to abscisic acid and high osmoticum in tobacco BY-2 cell cultures. Plant Sci 138: 27–34.
Chang TC and Tsai YF. (1986) Quantitative determination of starch in plant storage tissues by starch degrading enzymes. J Chin Agri Chem Soc 24: 392–396.
Chang S, Puryear J,and Cairney J. (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11: 113–116.
Cheng L, Fuchigami LH and Breen PJ. (2000) Light absorption and partitioning in relation to nitrogen content in ‘Fuji’ apple leaves. J Amer Soci Hort Sci 125: 581–587.
Crafts-Brandner SJ and Salvucci ME. (2002) Sensitivity of photosynthesis in a C4 plant, Maize, to heat stress. Plant Physiol 129: 1773–1780.
Demmig-Adams B, AdamsⅢ WW, Barker DH, Logan BA, Bowlong DR and Verhoeven AS. (1996) Using chlorophyll fluorescence to assess the fraction of absorbed light allocsted to thermal dissipation of excess excitation. Physiol Plantarum 98: 253–264.
Dubey RS and Singh AK. (1999) Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolising enzymes en rice plants. Biol Plant 42: 233–239.
Dwivedi U, Sharma M and Bhardwaj R. (1995) Down regulation of photosynthesis in Artabotrys hexapetatus by high light. Photosyn Res 46: 393–397.
Ehness R, Ecker M, Godt D and Roitsch T. (1997) Glucose and stress independently regulate source/sink relations and defence mechanisms via signal transduction pathways involving protein phosphorylation. Plant Cell 9: 1825–1841.
Elstner EF. (1982) Oxygen activation and oxygen toxicity. Annu Rev
Plant Physiol 33: 73–96.
Emerson R, Chalmers R and Cederstrand C. (1957) Some factors influencing the long-wave length limit of photosynthesis. Proc Natl Acad Sci USA 43: 133–143.
Eskins K, Duysen M, Dybas L and McCarthy S. (1985) Light quality effects on corn chloroplast development. Plant Physiol 77: 29–34.
Fernie AR, Willmitzer L and Trethewey RN. (2002) Sucrose to starch: atransition in molecular plant physiology. Trends Plant Sci 7: 35–41.
Ferris R and Taylor G. (1994) Stomatal characteristics of four native herbs following exposure to elevated CO2. Ann Bot 73: 447–453.
Foyer CH and Halliwell B. (1976) The presence of glutathione and glutathione reductase in chloroplasts. A proposal role in ascorbic acid metabolism. Planta 133: 21–25.
Gamble PE and Burke JJ. (1984) Effect of water stress on the chloroplast antioxidant system: alterations in glutathione reductase activity. Plant Physiol 76: 615–621.
Gechev T, Gadjev I, Van Breusegem F, Inze´ D and Dukiandjiev S, Toneva V and Minkov I. (2002) Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cel Mol Life Sci 59: 708–714.
Gechev TS, Breusegem FV, Stone JM, Denev I and Laloi C. (2006) Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bio Essays 28:1091–1101.
Gill PK, Sharma AD, Singh P and Bhullar SS. (2001) Effect of various abiotic stresses on the growth soluble sugars and water relations of sorghum seedlings grown in light and darkness. Bulg J Plant Physiol 27: 72–84.
Guy CL. (1990) Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol Plant Mol Biol 41: 187–223.
Heo JW, Lee CW and Paek KY. (2006) Influence of mixed LED radiation on the growth of annual plants. J Plant Biol 49: 286–290.
Heraud P and Beardall J. (2000) Changes in chlorophyll fluorescence during exposure of Dunaliella tertiolecate to UV radiation indicate a dynamic interaction between damage and repair processes. Photosyn Res 63: 123–134.
Herbers K and Sonnewald U. (1998) Molecular determinants of sink strength. Curr Opin Plant Biol 1: 207–216.
Hopkins WC. (1999) Introduction to Plant Physiology. 2nd ed. New York: John Wiley & Sons, Inc. pp. 173–186.
Hossain MA, Nakano Y and Asada K. (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25: 385–395.
Huber SC and Huber JL. (1992) Role of sucrose-phosphate synthase in sucrose metabolism in leaves. Plant Physiol 99: 1275–1278.
Huber SC and Huber JL. (1996) Role and regulation of sucrose- phosphate synthase in higher plants. Annu Rev Plant Physiol Plant Mol Biol 47: 431–444.
Huber SC, McMichael RW, Huber JL, Bachmann M, Yamamoto YT and Conkling MA. (1995) Light regulation of sucrose synthesis: role of protein phosphorylation and possible involvement of cytosolic [Ca2+]. In Carbon Partitioning and Source-Sink Interactions in Plants, ed. MAMadore, W Lucas, pp. 35–44. Rockville, MD: Am. Soc. Plant Physiol.
Huber SC, Nielsen TH, Huber JLA and Pharr DM. (1989) Variation among species in light activation of sucrose-phosphate synthase. Plant Cell Physiol 30: 277–285.
Jang JC and Sheen J. (1994) Sugar sensing in higher plants. Plant Cell 6: 1665–1679.
Jefferson PG and Muri R. (2007) Competition, light quality and seeding growth of Russian wildrye grass (Psathyrostachys juncea). Acta Agronomica Hungarica 55: 49–60.
Jiang ZY, Woollard AC and Wolff SP. (1990) Hydrogen peroxide production during experimental protein glycation. FEBS Lett 268: 69–71.
Karavatas S and Manetas Y. (1999) Seasonal patterns of photosystemⅡ photochemical efficiency in evergreen sclerophylls and drought semi-deciduous shrubs under Mediterranean field conditions. Photosynthetica 36: 41–49.
Kato M and Shimizu S. (1987) Chlorophyll metabolism in higher plants.Ⅶ. Chlorophyll degradation in senescing tobacco leaves; phenolicdependentperoxidative degradation. Can J Bot 65: 729–735.
Kim SJ, Hahn EJ, Heo JW and Paek KY. (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Scientia Hort 101: 143–151.
Krause GH and Weis E. (1992) Chlorophyll fluorescence and photosynthesis: The basis. Ann Rev Plant Physiol Plant Mol Biol 42: 313–349.
Lazar D. (1999) Review: Chlorophyll a fluorescence induction. Biochim Biophys Acta 1412: 1–28.
Lee MH, Yang CC, Wang HL, and Lee PD. (2003) Regulation of sucrose phosphate synthase of the sweet potato callus is related to illumination and osmotic stress. Bot Bull Acad Sin 44: 257–265.
Lee SH, Tewari RK, Hahn EJ, and Paek KY. (2007) Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania Somnifera (L.) Dunal. plantlets. Plant Cell Tiss Organ Cult 90: 141–151.
Li XP, Gilmore AM, Caffarri S, Bassi R, Golan T, Kramer D and Niyogi KK. (2004) Regulation of photosynthetic light harvesting involoves intrathylakoid lumen pH sensing by the PsbS protein. J Biol Chem 279: 22866–22874.
Li XP, Gilmore AM and Niyogi KK. (2002a). Molecular and global time-resolved analysis of a PsbS gene dosage effect on pH- and xanthophyll cycle-dependent nonphotochemical quenching in photosystemⅡ. J Biol Chem 277: 33590–33597.
Li XP, Phippard A, Pasari J and Niyogi KK. (2002b) Structure function analysis of photosystemⅡ subunit S (PsbS) in vivo, Funct Plant Biol 29: 1131–1139.
Li XQ and Zhang DP. (2003) Gene expression activity and pathway selection for sucrose metabolism in developing storage root of sweet potato. Plant Cell Physiol 44: 630–636.
Liu X, Williams CE, Nemacheck JA, Wang H, Subramanyam S, Zheng C, and Chen MS. (2010) Reactive oxygen species are involved in plant defense against a gall midge. Plant Physiol 152: 985–999.
Mano J, Ushimaru T and Asada K. (1997) Ascorbate in thylakoid lumen as an endogenous electron donor to photosystemⅡ: protection of thylakoids from photoinhibition and regeneration of ascorbate in stroma by dehydroascorbate reductase. Photosynth Res 53: 197–204.
Matsushita K and Uritani I. (1974) Change in invertase activity of sweet potato in response to wounding and purification and properties of its invertases. Plant Physiol 54: 60–66.
Michiyama H, Arikuni M, Hirano T and Hayashi H. (2003) Influence of day length before and after the start of anthesis on the growth flowering and seed-setting in common buckwheat (Fagopyrum esculentum Moench). Plant Prod Sci 6: 235–242.
Michiyama H, Tsuchimoto K, Tani KI, Hirano T, Hayashi H and Campbell C. (2005) Influence of day length on stem growth, flowering, morphology of flower clusters and seed set in buckwheat (Fagopyrum esculentum Moench). Plant Prod Sci 8: 44–50.
Van Mieghem F, Brettel K, Hillmann B, Kamlowski A, Rutherford AW and Schlodder E. (1995) Charge recombination reactions in photosystemⅡ.1.Yields, recombination pathway, and kinetics of the primary pair. Biochem 34: 4798–4813.
Miller AJ and Sanders D. (1987) Depletion of cytosolic free calcium induced by photosynthesis. Nature 326: 397–400.
Miyashita Y, Kitaya Y, Kozai T and Kimura T. (1995) Effects of red and far-red light on the growth and morphology of potato plantlets: using light emitting diode as a source for micropropagation. Acta Hortic 393: 710–715.
Monk LS, Fagerstedt KV and Crawford RMM. (1989) Oxygen toxicity and superoxide dismutase as an antioxidant in physiological stress. Physiol Plant 76: 456–459.
Murashige T and Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Müller P, Li XP and Niyogi KK. (2001) Non-photochemical quenching. A. response to excess light energy. Plant Physiol 125: 1558–1566.
Nakano Y and Asada K. (1981) Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867–880.
Nanda AK, Andrio E, Marino D, Pauly N and Dunand C. (2010) Reactive oxygen species during plant-microorganism early interactions. J Integrative Plant Biol 52: 195–204.
Nelson N. (1944) A photometric adaptation of the somogyi method for the determination of glucose. J Biol Chem 153: 375–380.
Nhut DT and Nam NB. (2010) Light-emitting diodes (LEDs): an artificial lighting source for biological studies. IFMBE Proceedings 27: 134–139.
Niyogi KK, Li XP, Rosenberg V and Jung HS. (2005) Is PsbS the site of non-photochemical quenching in photosynthesis? J Exp Bot 56: 375–382.
Ottander C, Campbell D and Öquist G. (1995) Seasonal changes in photosystemⅡ organization and pigment composition in Pinus sylvestris. Planta 197: 176–183.
Paoletti F and Mocali A. (1990) Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation. Methods Enzymol 186: 209–220.
Pastori GM and Trippi VS. (1992) Oxidative stress induces high rate of glutathione reductase synthesis in a drought-resistant maize strain. Plant Cell Physiol 33: 957–961.
Pastori GM and Trippi VS. (1993) Antioxidative protection in a drought-resistant maize strain during leaf senescence. Physiol Plant 87: 227–231.
Pei ZM, Murata Y, Benning G, Thomine S, Klusener B, Allen GJ, Grill E and Schroeder JI. (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406: 731–734.
Pierik R, Keuskamp DH, Sasidharan R, Djakovic-Petrovic T, de Wit M, and Voesenek LA. (2009) Light quality controls shoot elongation through regulation of multiple hormones. Plant Signal Behav 4: 755–756.
Poudel PR, Kataoka I and Mochioka R. (2008) Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Organ Cult 92:147–153.
Prasad TK, Anderson MD, Martin BA and Stewart CR. (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatoryrole for hydrogen peroxide. Plant Cell 6: 65–74.
Qin YH, Zhang SL, Syed A, Zhang L, Qin QP, Chen KS and Xu CG. (2005) Regeneration mechanism of Toyonoka strawberry under different color plastic films. Plant Sci 168: 1425–1431.
Quiles MJ. (2005) Photoinhibition of photosystemsⅠ and Ⅱ using chlorophyll fluorescence measurements. J Biological Educ 39: 136–138.
Ramos MLG, Gordon AJ, Minchin FR, Sprent JI and Parsons R. (1999) Effect of water stress on nodule physiology and biochemistry of a drought tolerant dultivar of common bean (Phaseolus vulgaris L.). Ann Bot 83: 57–63.
Röhacek K and Bartak M. (1999) Technique of the modulated chlorophyll fluorescence: basic concepts,useful parameter,and some applications. Photosynthetica 37: 339–363.
Salerno GL and Curatti L. (2003) Origin of sucrose metabolism in higher plants: when, how and why? TRENDS Plant Sci 8: 63–69.
Sasidharan R, Chinnappa CC, Voesenek LA and Pierik R. (2008) The regulation of cell wall extensibility during shade avoidance: a study using two contrasting ecotypes of Stellaria longipes. Plant Physiol 148: 1557–1569.
Schuerger AC and Brown CS. (1997) Spectral quality affects disease development of three pathogens on hydroponically grown plants. HortScience 32: 96–100.
Smirnoff N and Colombé SV. (1988) Drought influences the activity of enzymes of the chloroplast hydrogen peroxide scavenging system. J Exp Bot 39: 1097–1108.
Strand Å, Hurry V, Henkes S, Huner N, Gustafsson P, Gardeström P and Stitt M. (1999) Acclimation of Arabidopsis leaves developing at low temperatures. Increasing cytoplasmic volume accompanies increased activities of enzymes in the Calvin cycle and in the sucrose- biosynthesis pathway. Plant Physiol 119: 1387–1397.
Stuefer JF and Huber H. (1998) Differential effects of light quantity and spectral light quality on growth, morphology and development of two stoloniferous Potentilla species. Oecologia 117: 1–8.
Sturm A. (1999) Invertases. Primary structures, functions, and roles in plant development and Suc partitioning. Plant Physiol 121: 1–8.
Taiz L and Zeiger E. (2002) There are four major groups of flavonoids. Plant Physiology. pp.294, Sinauer Associates, Inc., USA.
Tang T,Xie H, Wang Y, Lu B and Liang J. (2009) The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.). J Expl Bot 60: 2641–2652.
Tian M, Gu Q and Zhu MY. (2003) The involvement of hydrogen peroxide and antioxidant enzymes in the process of shoot organogenesis of strawberry callus. Plant Sci 165: 701–707.
Varsha Gupta and Baishnab C Tripathy. (2010) Effect of light quality on chlorophyll accumulation and protein expression in wheat (Triticum aestivum L.) Seedlings. Intl J Biotechnol Biochem 6: 521–536.
Wang H, Gu M, Cui J, Shi K, Zhou Y and Yu J. (2009) Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J Photoch Photobio B 96: 30–37.
Wang HL, Lee PD, Chen WL, Huang DJ and Su JC. (2000) Osmotic stress-induced changes of sucrose metabolism in cultured sweet potato cells. J Exp Bot 51: 1991–1999.
Wang HL, Lee PD, Liu LF and Su JC. (1999) Effect of sorbitol induced osmotic stress on the changes of carbohydrate and free amino acids pools in sweet potato cell suspension cultures. Bot Bull Acad Sin 40: 219–225.
Weiner H, Weiner H and Stitt M. (1993) Sucrose-phosphate synthase phosphatase, a type 2A protein phosphatase, changes its sensitivity towards inhibition by inorganic phosphate in spinach leaves. FEBS Lett 333: 159–164.
Wu MC, Hou CY, Jiang CM, Wang YT, Wang CY, Chen HH and Chang HM. (2007) A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. Food Chem 101: 1753–1758.
Yelle S, Hewitt JD, Robinson NL, Damon S and Bennett AB. (1988) Sink metabolism in tomato fruit :Ⅲ. analysis of carbohydrate assimilation in a wild species. Plant Physiol 87: 737–740.
Zeiger E. (1983) The biology of stomatal guard cells. Annu Rev Plant Physiol 34: 441–475.
Zheng J, Hu MJ and Guo YP. (2008) Regulation of photosynthesis by light quality and its mechanism in plants. Ying Yong Sheng Tai Xue Bao 19: 1619–1624.