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研究生：	張嘉芸 Chia-Yun Chang
論文名稱：	阿達瑪轉換/毛細管電泳法適用之奈米流量進樣器的開發與研究 Development of a nano-controlled sample injection device for use in Hadamard transform/capillary electrophoresis (HT/CE)
指導教授：	林震煌 Lin, Cheng-Huang
學位類別：	碩士 Master
系所名稱：	化學系 Department of Chemistry
論文出版年：	2011
畢業學年度：	99
語文別：	中文
論文頁數：	87
中文關鍵詞：	阿達瑪轉換-毛細管電泳法、維他命B2
論文種類：	學術論文
相關次數：	點閱：122 下載：2
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本研究設計一個新的奈米流量（nano-controlled）進樣器，並成功結合阿達瑪轉換法（Hadamard transform）與毛細管電泳法（capillary electrophoresis）。其中，首次利用壓克力Tee型連接頭將進樣器及毛細管電泳裝置連結。
　　實驗過程為將10 μL的注射針置於注射式幫浦（syringe pump）上，再NI（National Instruments）PCI-6221程式控制注射式幫浦內部之步進馬達（stepping motor）轉動步數，進一步控制進樣體積。當設定步進馬達轉動步數為750~20000步（進樣體積為1.3 nL~49.4 nL）的範圍時，具有良好的線性關係（R2 =0.9927）。本研究以核黃素（又稱維他命B2）作為分析物，並以藍光雷射（波長473 nm, 100 mW）作為激發光源。當分析物推入壓克力Tee且經電泳分離後，通過偵測窗時，由雷射激發且放出螢光，最後以光電倍增管（PMT）作偵測。在相同的進樣條件下，以阿達瑪127、255次序列分別進行實驗，訊號雜訊比可得到5.3及7.9倍的改良效果，與理論值（5.6與8）相當符合。最後，本實驗嘗試以阿達瑪127次序列進樣與毛細管線上濃縮技術進行結合，結果顯示其S/N比增加5.2倍，與理論值相當接近。
關鍵字：阿達瑪轉換／毛細管電泳法、維他命B2

A novel nano-controlled sample injection device for use in Hadamard transform/capillary electrophoresis (HT/CE) was successfully developed. Instead of commercial Tee products, an acrylic-Tee connector was well designed and made in-house. Three pieces of capillaries (used for sample solution and buffer solutions, respectively) can be tightly connected together with very low dead-volume. The sample solution was placed in a syringe injector (size, 10 µL) and was pushed out by a stepping motor which was controlled by a personal computer through a NI (National Instruments) PCI-6221 device. The volume of sample injection can be well controlled and calculated based on the steps (750- ~ 20,000-steps) of the stepping motor, corresponding to the injected volume of 1.3 nL ~ 49.4 nL. A riboflavin solution and a blue diode laser (wavelength, 473 nm; 100 mW) were selected as a model compound and the light source, respectively. Compared with a conventional single injection method, the S/N ratios were substantially improved after inverse Hadamard transformation of the encoded chromatogram. Under optimized conditions, when Hadamard matrices of 127 and 255 were used, the S/N ratios of the signals for riboflavin (concentration level, 0.03 ppm) were substantially improved to 5.3- and 7.9-fold, respectively, and those improvements are in good agreement with those obtained by theory (5.6- and 8.0-fold).

中文摘要    Ⅰ
英文摘要    Ⅱ
目錄    Ⅲ
圖目錄    Ⅴ
表目錄    Ⅵ

第一章、緒論    1
1-1    研究目的    1
1-2    分析物簡介    3
1-2-1　維他命B2     3
1-2-2　孔雀石綠染料    6

第二章、分析方法與原理    8
2-1  毛細管電泳層析法之分離模式    8
    2-1-1  毛細管區帶電泳（CZE）    9
    2-1-2  微胞電動層析法（MEKC）    12
2-2   毛細管線上濃縮技術    16
    2-2-1  毛細管電泳線上堆積法（stacking）    18
2-3    阿達瑪矩陣原理    20
    2-3-1  矩陣起源    21
    2-3-2  阿達瑪轉換法    22
    2-3-3  LabVIEW 操作程式    29
    2-3-4  阿達瑪轉換提高S/N值的理論值    31
2-4   阿達瑪矩陣轉換在毛細管電泳上的應用    34

第三章、實驗    35
3-1    壓克力Tee型連接頭的製作    35
3-2    進樣裝置    37
    3-2-1  機械式進樣    37
    3-2-2  氣動式進樣    39
3-3    實驗儀器裝置    42
    3-3-1  自組式毛細管電泳／雷射誘導螢光分析儀（CE- LIF）    42
    3-3-2  自組式毛細管電泳／拉曼分析儀（CE- Raman）    44
3-4    儀器及週邊設備列表    46
3-5    使用藥品列表    50
3-6    氣動式進樣－毛細管電泳／拉曼分析儀之實驗條件    51
    3-6-1  氣動式進樣之條件    51
    3-6-2  毛細管區帶電泳（CZE）電泳條件的確立與配製    51

3-7    機械式進樣－毛細管電泳／雷射誘導螢光儀之實驗條件    52
    3-7-1  毛細管區帶電泳（CZE）電泳條件的確立與配製    52
    3-7-2  線上濃縮技術CZE-stacking條件的確立與配製    53

第四章、結果與討論    54
4-1    市售三向連接頭與壓克力Tee型連接頭之比較    54
4-2    氣動式進樣－毛細管電泳／拉曼分析儀    56
    4-2-1  氣動式進樣－毛細管電泳／拉曼分析儀之電泳圖探討    56
    4-2-2  壓力式進樣的穩定度    61
4-3   機械式進樣－毛細管電泳／雷射誘導螢光分析儀    64
    4-3-1  緩衝溶液的選擇    64
    4-3-2  步進馬達之穩定度    66
    4-3-3  機械進樣之穩定度    68
    4-3-4  以阿達瑪序列進樣及轉換結果與理論值比較    72
    4-3-5  以阿達瑪序列進樣搭配CZE-stacking    76

第五章、結論    83

參考文獻    84
                                

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