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研究生: 陳婷妤
Chen, Ting-Yu
論文名稱: 中草藥純物質 Baicalein 衍生物作為治療阿茲海默氏症藥物開發之探討
The Development of Drugs from Baicalein Derivatives for Alzheimer's Disease Treatment
指導教授: 林炎壽
Lin, Yenshou
口試委員: 林炎壽
Lin, Yenshou
李冠群
Lee, Guan-Chiun
楊文欽
Yang, Wen-Chin
口試日期: 2023/05/05
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2023
畢業學年度: 111
語文別: 中文
論文頁數: 64
中文關鍵詞: 阿茲海默氏症中草藥β-類澱粉蛋白黃芩素麩胺酸
研究方法: 實驗設計法
DOI URL: http://doi.org/10.6345/NTNU202300953
論文種類: 學術論文
相關次數: 點閱:91下載:0
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  • 目錄I 摘要 III ABSTRACT IV 第一章、 緒論 1 一. 阿茲海默氏症 1 二. 𝛽-類澱粉蛋白之生成 2 三. 𝛽-類澱粉蛋白之致病之分子機轉3 四. 現今治療阿茲海默氏症之臨床藥物4 五. 黃芩素於阿茲海默氏症上的治療效果5 第二章、 研究動機與目的6 第三章、 材料與實驗方法 7 一. 藥品7 二. 小鼠大腦皮質神經細胞初級培養 8 三. 細胞免疫螢光染色法9 四. 細胞存活度檢測9 五. 𝛽-類澱粉蛋白寡聚體製備10 六. 蛋白質斑點印漬分析 10 七. DIBAC4(3) 膜電位螢光染劑進行藥物篩選 11 八. 利用 FURA2-AM 檢測藥物對鈣離子的影響 11 九. 西方墨點法 12 十. 實驗動物與藥物給予 13 十一. 基因型檢測 14 十二. 新物體識別測試 (NOVEL OBJECT RECOGNITION TEST) 14 十三. 莫氏水迷津實驗 (MORRIS WATER MAZE TEST) 15 十四. 巴恩斯迷宮 (BARNES MAZE TEST) 16 十五. 檢測血清中 GOT、GPT 與肝臟、腎臟組織 H&E 染色切片17 十六. 統計分析 17 第四章、 結果 19 一. BE 衍生物對神經細胞之毒性測試19 二. BE1 不能減緩 A𝛽寡聚體誘導的神經不正常去極化 19 三. BE2 能減緩 A𝛽寡聚體誘導的神經不正常去極化 20 四. BE2 相較於 BE 具有較低的有效濃度能減緩 AMPA 誘導的神經不正常去極化21 五. BE2 與 BE 皆能減緩過量的 NMDA 誘導的神經不正常去極化 22 六. BE2 相較於 BE 能更有效的減緩過量的 NMDA 誘導的鈣離子流入23 七. BE2 能改善 A𝛽刺激對 ERK 訊息傳遞路徑影響 24 八. BE2 能改善過量的 NMDA 刺激對 ERK 訊息傳遞路徑影響 24 九. BE2 對於小鼠並不會影響其體重及一些基本生理參數 25 十. 給予六個月大 J20 小鼠 BE2 能改善其認知行為 26 十一. 給予六個月大 J20 小鼠 BE2 能改善其空間記憶行為 26 第五章、 討論 29 附錄 57 參考文獻61

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