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研究生: 顏長德
論文名稱: 低磁場核磁共振應用於奈米磁性粒子之免疫檢測
指導教授: 楊鴻昌
Yang, Shieh-Yueh
洪姮娥
Horng, Herng-Er
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2010
畢業學年度: 98
語文別: 中文
論文頁數: 49
中文關鍵詞: 核磁共振免疫檢測
英文關鍵詞: NMR, Immunoassay
論文種類: 學術論文
相關次數: 點閱:103下載:0
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  • 本研究我們嘗試用核磁共振原理將均勻分布在水中披覆著生物探針(bioprobe)的奈米磁性粒子與待測生物分子(biotarget),藉由結合後所形成的磁性叢集(magnetic cluster)會使水中氫原子核的橫向鬆弛時間(transverse relaxation time)改變,我們測量磁性叢集之磁共振頻譜訊號推出其線寬、T2與T2*等物理量變化,發展出一種嶄新生物標地物的分析方式。
    此外我們利用不同濃度的奈米磁性粒子製作不同的T1樣品,藉此模擬不同組織在我們系統下進行T1權重造影(T1-weighted image)的結果,用以評估將來要對生物組織做核磁共振造影的可行性與改進方向。

    第一章 緒論 3 第二章 實驗原理 2-1 低磁場之核磁共振介紹 5 2-2 核磁共振原理 2-2-1 核磁共振 6 2-2-2 縱向鬆弛與橫向鬆弛 8 2-3 應用NMR於磁性標記免疫檢測 14 2-4 C-反應蛋白(C-reactive Protein, CRP)簡介 16 第三章 實驗架構 3-1 系統架構介紹 17 3-2 預先極化線圈 (Pre-polarization coil) 19 3-3 冷卻水系統 22     3-4 接收線圈之最佳化 25 3-5 實驗參數之決定與系統穩定度 26 第四章 實驗結果與數據討論 4-1 磁性標記免疫檢測 4-1-1 利用柯西-勞倫茲分布特性計算線寬 29 4-1-2 NMR線寬變化之量測 32 4-2 奈米磁性粒子的其他應用 4-2-1 不同濃度磁性流體對水之T1量測 37 4-2-2 T1 -權重造影(T1-weighted image) 39 第五章 結論與未來展望 45 參考文獻 47

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