研究生: |
林暐智 Wei-Chih Lin |
---|---|
論文名稱: |
阿達瑪轉換法在液相層析/拉曼光譜法之應用 Applications of Hadamard Transform-Liquid Chromatography based on Raman spectrometry |
指導教授: |
林震煌
Lin, Cheng-Huang |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2011 |
畢業學年度: | 99 |
語文別: | 中文 |
論文頁數: | 122 |
中文關鍵詞: | 阿達瑪轉換 、拉曼光譜法 、液相層析法 |
英文關鍵詞: | Hadamard Transform, Raman spectrometry, Liquid Chromatography |
論文種類: | 學術論文 |
相關次數: | 點閱:181 下載:2 |
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本研究首次將阿達碼轉換(Hadamard transform)技術應用在液相層析/拉曼光譜法(liquid chromatography/Raman spectrometry, LC/RS)上。分析物溶液置於精密注射針內,配合注射泵(syringe pump)推擠進樣,在三向連接頭(Tee connector)中,與來自液相層析泵的流析溶劑匯流以後,共同經過層析分離管柱。經分離之分析物的拉曼訊號則由市售拉曼光譜儀(Renishaw Invia Raman spectroscope)收集。配合阿達瑪序碼(Hadamard code)的使用,由電腦可以控制注射泵的進樣順序,因此可以得到阿達碼-層析圖碼。此編碼之層析圖經過阿達碼轉換以後,可還原得到解碼之液相層析/拉曼光譜圖。實驗結果發現,此方法非常有助於提高雜訊比值,亦有助於提高偵測靈敏度。當以結晶紫(crystal violet, CV)作為測試樣品,編碼後的層析圖譜經由阿達瑪轉換後,訊號/雜訊比提高了7.29倍。這數值與理論值8.02 相當接近。再者,本研究同時發現,經過層析分離管柱後的分析物若能與奈米銀溶液再次匯流混合的話,藉由表面增強拉曼光現象,可提升拉曼散射光的強度,使分析物的的偵測極限下降。即使分析物的濃度為 500 ppb,依舊可觀察到清楚的拉曼光譜圖。
The technique of Hadamard transform (HT) was successfully coupled with liquid chromatography/Raman spectrometry (LC/RS), for the first time. Crystal violet (CV) and a commercial Raman system (Renishaw Invia Raman Spectroscope) were employed as a model sample and detection instrument, respectively. Sample injection device was aslo developed to achieve multiple sample injections for the use of Hadamard
transformation. The sample solution was placed in a reservoir and was pushed out by a commercial syringe pump, which was controlled by a personal computer. Compared with a conventional single injection method in LC, the S/N ratios were substantially improved after inverse Hadamard transformation of the encoded chromatogram. Under optimized conditions, when Hadamard matrix of 255 was used, the S/N
ratio of the signals for crystal violet (concentration level, 0.5 ppm) was substantially improved to 7.29-fold, and the improvements are in good agreement with those obtained by theory (8.0-fold). The limit of detect was further improved when the method of surface enhanced Raman
scattering (SERS) was applied.
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