研究生: |
廖安淑 An-Shu Liau |
---|---|
論文名稱: |
發展簡易的β-環糊精修飾毛細管電泳法對毒品與吸毒者尿液中甲基安非他命類光學異構物的分離與分析 The chiral separation of methamphetamine and related compounds in clandestine tablets and urine samples by beta-cyclodextrine modified capillary electrophoresis |
指導教授: |
林震煌
Lin, Cheng-Huang |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2004 |
畢業學年度: | 93 |
語文別: | 中文 |
論文頁數: | 109 |
中文關鍵詞: | β-環糊精 、毛細管電泳法 、甲基安非他命 、光學異構物 |
英文關鍵詞: | beta-cyclodextrine, capillary electrophoresis, methamphetamine, chiral separation |
論文種類: | 學術論文 |
相關次數: | 點閱:286 下載:13 |
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本研究的主要重點在於發展以毛細管電泳法,結合β-CD對鏡像異構物的選擇能力,成功的將 (±)-甲基安非他命、(±)-麻黃酮、(±)-麻黃素、(±)-假麻黃素分離開來,並可用於鑑定這些鏡像異構物在毒品粉末和吸毒者尿液中的分布。在研究過程中,比較了β-CD濃度大小、溫度高低、電壓大小和有機修飾劑在緩衝溶液中的比例等變因對電泳結果的影響,以找出最佳的電泳條件。本研究成果,發現以CE/UV法來分離偵測這些鏡像異構物,不但快速簡便,而且解析度高,可彌補GC/MS在分析鏡像異構物時必須事先作複雜的衍生工作才能達成目的之不足,所以此方法對於法庭檢驗和臨床分析上有很大的幫助。
本研究找到最佳分離上述分析物的緩衝溶液為:磷酸鹽濃度150 mM、β-CD濃度17.5 mM、溶劑比例H2O/ACN = 95/5;最佳的電泳條件為:溫度範圍在15 ~ 30 C之間,電壓範圍在20 ~ 25 kV之間。如此就能不需要做任何的樣品前處理工作,即能在20分鐘的時間之內完成分析檢驗工作,偵測極限約可達1 ppm (S/N = 3/1)。
The chiral separation of (±)-methamphetamine, (±)-methcathinone, (±)-ephedrine and (±)-pseudoephedrine by means of beta-cyclodextrine modified capillary electrophoresis is described. The distribution of enantiomers in clandestine tablets and urine samples were identified. Several electrophoretic parameters, such as the concentration of -cyclodextrin, temperature, the applied voltage and the amount of organic solvent required for successful separation were optimized. The method, as described herein, represents a good complementary method to GC/MS for use in forensic and clinical analysis.
The optimum CE conditions for the analysis of these analytes was achieved using a mixture of water-acetonitril solution (95:5, v/v) containing phosphate (150 mM), -CD (17.5 mM) at 15 ~30 C; applied voltage: 20 ~ 25 kV. This method proposed here can provide results in less than 20 min without any complicated pre-treatments and provided a ~1 ppm detected limit for methamphetamine related compounds.
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