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研究生: 陳冠甫
Kuan-Fu Chen
論文名稱: 微波輔助螢光標定毛細管電泳法對安非他命狡詐家濫用藥物的分離與偵測
A microwave-assisted fluorescent labeling method for the separation and detection of amphetamine-like designer drugs by capillary electrophoresis.
指導教授: 林震煌
Lin, Cheng-Huang
學位類別: 碩士
Master
系所名稱: 化學系
Department of Chemistry
論文出版年: 2013
畢業學年度: 101
語文別: 中文
論文頁數: 63
中文關鍵詞: 毛細管電泳微波衍生安非他命濫用藥物苯乙胺類FITC
英文關鍵詞: CE-LIF, microwave-assisted, amphetamine-like designer drugs, phenethylamine designer drugs
論文種類: 學術論文
相關次數: 點閱:120下載:2
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  • 本研究利用微波輔助螢光標定應用於毛細管電泳分析方法,用來分析鄰、間、對-氯安非他命和鄰、間、對-氟安非他命這六種安非他命濫用藥物與 2,5-dimethoxy- 4-ethylthio-phenethylamine(2C-T-2)、2,5-dimethoxy-4-(n)-propylthiophen- ethylamine(2C-T-7)、4-chloro-2,5-
    dimethoxyphenethylamine(2C-C)、4-bromo-2,5-dimethoxy-phenethylamine(2C-B)及 2,5-dimethoxy-4-iodo- phenethylamine(2C-I)五種 2C 系列的苯乙胺類毒品。利用 FITC(fluorescein isothiocyanate isomer I)當作螢光標記並使用藍光雷射(473 nm)作激發光源進行偵測。在過去使用 FITC 作衍生劑的衍生時間至少需要 20 小時,現今使用微波輔助衍生所需時間約 5 分鐘即可完成。

    A microwave-assisted fluorescence labeling method for use in CE-LIF (capillary electrophoresis-laser induced fluorescence) is described. Six amphetamine-like designer drugs, namely, o-, m-, p-chloro- and o-, m-, p-fluoro-amphetamine derivatives, and five 2C-series of phenethylamine designer drugs, including 2,5-dimethoxy-4-ethylthio-phenethylamine (2C-T-2), 2,5-dimethoxy-4-(n)-propylthiophenethylamine(2C-T-7), 4-chloro-2,5-dimethoxy-phenethylamine(2C-C), 4-bromo-2,5-dimethoxy- phenethylamine(2C-B), 2,5-dimethoxy-4-iodo-phenethylamine(2C-I) derivatives, were synthesized and used as model compounds. FITC (fluoresceinisothiocyanate isomer I) and a blue-laser were used as the fluorescent labeling reagent and excitation source, respectively. When a microwave oven was used, the reaction was complete within ~ 5 min,while the classical method required at least 20 h(usually, an overnight reaction)

    目錄 第一章 緒論 1 1-1 研究目的 1 1-2 研究介紹 2 1-3 分析物簡介 3 1-3-1安非他命 3 第二章 分析方法及原理 10 2-1 毛細管電泳分析法之發展 10 2-2毛細管電泳分離模式 11 2-2-1毛細管區帶電泳 12 2-2-2 微胞電動層析法 13 2-2-3毛細管電泳線上掃集法 14 2-3 微波簡介 15 2-4 微波理論 16 第三章 儀器、藥品與實驗方法 18 3-1自組式毛細管電泳/雷射誘導螢光分析儀 18 3-2儀器及周邊設備列表 20 3-3藥品列表 24 3-4 FITC衍生物製備 27 3-4-1安非他命衍生 27 3-4-2 苯乙胺 2C 系列衍生 28 第四章 結果與討論 29 4-1衍生試劑 FITC 與鹵化安非他命反應放置衍生之探討 30 4-2使用光電倍增管偵測鹵化安非他命分析物產生的螢光 33 4-3衍生試劑 FITC 與 2C 系列放置衍生反應之探討 39 4-4使用光電倍增管偵測 2C 系列分析物產生的螢光 41 4-5衍生試劑 FITC 與鹵化安非他命反應微波衍生之探討 45 4-6衍生試劑 FITC 與 2C 系列反應微波衍生之探討 50 4-7放置衍生與微波衍生的比較 54 第五章 結論 57 參考文獻 58 論文發表 63

    參考文獻
    [1] T. Ishida, K. Kudo, A. Kiyoshima, H. Inoue, A. Tsuji, N. Ikeda, J. Chromatogr. B 823 (2005) 47.
    [2] R. Kikura-Hanajiri, M. Hayashi, K. Saisho, Y. Goda, J. Chromatogr. B 825 (2005) 29.
    [3] J. M. Wilson, F. McGeorge, S. Smolinske, R. Meatherall, Forensic Sci. Int. 148 (2005) 31.
    [4] M. Takahashi, M. Nagashima, J. Suzuki, T. Seto, I. Yasuda, T. Yoshida, J. Health Sci. 54 (2008) 89.
    [5] T. Kanamori, M. Nagashima, J. Suzuki, T. Seto, I. Yasuda, T. Sci. Int. 148 (2005) 31.
    [6] T. Kamata, M. Katagi, H. Kamata, A. Miki, N. Shima, K. Zaitsu, M. Nishikawa, H. Tsuchihashi, J. Health Sci. 53 (2007) 585.
    [7] S. D. Brandt, D. Mansell, S. Freeman, I.A. Fleet, J. F. Alder, J. Pharm. Biomed. Anal. 41 (2006) 872.
    [8] T. Kamata, M. Katagi, H. Tsuchihashi, Forensic Toxicol. 28 (2010) 1.
    [9] E. Tanaka, T. Kamata, M. Katagi, H. Tsuchihashi, K. Honda, Forensic Sci. Int. 163 (2006) 152.
    [10] S. Laks, A. Pelander, E. Vuori, E. Ali-Tolppa, E. Sippola, I. Ojanpera, Anal. Chem. 76 (2004) 7375.
    [11] N. D. Numan, Danielson, Anal. Chem. Acta 460 (2002) 49.
    [12] S. D. Brandt, S. Freeman, I. A. Fleet, P. McGaph, J. F. Alder, Analyst 130 (2005) 330.
    [13] J. Choi, C. Kim, J. M. Choi, Electrophoresis. 19 (1998) 2950.
    [14] N. Kuroda, R. Nomura, O. Al-Dirbashi, S. Akiyama, K. Nakashima, J. Chromatogr. A. 798 (1998) 325.
    [15] J. Choi, C. Kim, M. J. Choi, J. Chromatoger. B. 705 (1998) 277.
    [16] A. Ramseier, F. von Heeren, W. Thormann, Electrophoresis. 19 (1998) 2967.
    [17] L. Zhang, R. Wang, Y. Q. Yu,Y. R. Zhang, J. Chromatogr. B 857 (2007) 130.
    [18] UTRILLA, P., Ars Pharmaceutica 2000, 41, 67-77.
    [19] I. Lapin, CNS Drug rev. 2001, 7, 471-481.
    [20] A. Shulgin, PiHKAL, A Chemical Love Story, Transform Press, Berkeley, fifth printing, CA 94712, 2000, p. 557.
    [21] F. Kohlrausch, Ann. Phys. Chem. 62 (1897) 209. A. Tiselius, Trans. Faraday Soc. 33 (1937) 524.
    [22] Tiselius, Trans. Faraday Soc. 33 (1937) 524.
    [23] S. Hjerten, Chromatogr. Rev. 9 (1967) 122.
    [24] R. Virtanen, Acta Polytechnica Scand. Chem. 123 (1974) 1.
    [25] J. W. Jorgenson, K. D. Lukacs, J. Chromatogr. 218 (1981) 209.
    [26] J. W. Jorgenson, K. D. Lukacs, Anal. Chem. 53 (1981) 1298.
    [27] S. Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111.
    [28] S. Hjerten, M. D. Zhu, J. Chromatogr. 346 (1985) 265.
    [29] S. Hjerten, J. L. Liao, K. Yao, J. Chromatogr. 387 (1987) 127.
    [30] S. Cohen, B. L. Karger, J. Chromatogr. 397 (1987) 409.
    [31] M. M. Dittmann, G. P. Rozing, J. Chromatogr. A 744 (1996) 63.
    [32] C. Yan, R. Dadoo, R. N. Zare, D. J. Rakestraw, D. S. Anex, Anal. Chem. 68 (1996) 2726.
    [33] D. N. Heiger, Hewlett-Packard Company Publication Number 12-5091-6199E.
    [34] H. Z. Helmholtz, Annal. Phys. Chem. 7 (1879) 337.
    [35] D. N. Heiger, High Performance Capillary Electrophoresis - An Introduction, 1992, 2nd edition.
    [36] J.W. Joegenson, K. D. Lukacs, J. Chromatogr. 218 (1981) 209.
    [37] J.W. Joegenson, K. D. Lukacs, Anal. Chem. 53 (1981) 1298.
    [38] K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111.
    [39] S. Terabe, K. Otsuka, T. Ando, Anal. Chem. 57 (1985) 834.
    [40] K. H. Row, W. H. Griest, M. P. Maskarienc, J. Chromatogr. 409 (1987) 193.
    [41] B. L. Cohen, Karger, J. Chromatogr. 397 (1987) 409.
    [42] S. Hjerten, M. D. Zhu, J. Chromatogr. 346 (1985) 265.
    [43] S. Hjerten, J. L. Liao, K. Yao, J. Chromatogr. 387 (1987) 127.
    [44] S. Hjerten, K. Elenbring, F. Kilar, J. Liao, A. J. C. Chen, C. J. Siebert, M. Zhu, J. Chromatogr. 403 (1987) 47.
    [45] F. Foret, E. Szoko, B. L. Karger, J. Chromatogr. 608 (1992) 3.
    [46] C. Schwer, F. Lottspeich, J. Chromatogr. 623 (1992) 345.
    [47] M. Mazereeuw, U. R. Tjaden, N. J. Reinhoud, J. Chromatogr. Sci. 33 (1995) 686.
    [48] X. Huang, R. N. Zare, Anal. Chem. 63 (1991) 2193.
    [49] R. D. Holland, M. J. Sepaniak, Anal. Chem. 65 (1993) 1140.
    [50] X. Huang, M. J. Gordon, R. N. Zare, Anal. Chem. 60 (1993) 375.
    [51] R. T. Kennedy, J. W. Gorgenson, Anal. Chem. 61 (1989) 1128.
    [52] D. N. Heiger, Hewlett-Packard Company Publication Number 12-5091-6199E.
    [53] H. Z. Helmholtz, Anal. Phys. Chem. 7 (1897) 337.
    [54] B. Krattiger, G. J. M. Bruin, A. E. Bruin, Anal. Chem. 66 (1994) 1.
    [55] M. Stefansson, M. Novotny, Anal. Chem. 66 (1994) 1134.
    [56] Y. Kim, M.D. Morris, Anal. Chem. 66 (1994) 1168.
    [57] Z. Zhzo, A. Malik, M. L. Lee, Anal. Chem. 65 (1994) 2747.
    [58] R. L. Chien, M. G. Khaledi, High Performance Capillary Electrophoresis, Chapter 13, CRC Press, 1998.
    [59] Z. Liu, P. Sam, S. R. Sirimanne, P. C. McClure, J. Grainger, D. G. Patterson, J. Chromatogr. A 673 (1994) 125.
    [60] S.Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem. 56 (1984) 111.
    [61] K. R. Nielson, J. P. Foley, J. Chromatogr. A 686 (1994) 283.
    [62] J. P. Quirino, S. Terabe, J. Chromatogr. A 781 (1997) 119.
    [63] C. X. Zhang, W. Thormann, Anal. Chem. 70 (1998) 540
    [64] Z. K. Shihabi, J. Chromatogr. A 817 (1998) 25.
    [65] J. Palmer, N. J. Munro, J. P. Landers, Anal. Chem. 71 (1999), 1679.
    [66] J. P. Quirino, S. Terabe, Anal. Chem. 72 (2000) 1023.
    [67] Nunez, J. B. Kim, E. Moyano, M. T. Galceran, S. Terabe, J. Chromatogr. A 961 (2002) 65.
    [68] J. B. Kim, K. Otsuka, S. Terabe, J. Chromatogr. A 932 (2001) 129.
    [69] L. Zhu, C. Tu, H. K. Lee, Anal. Chem. 74 (2002) 5820.
    [70] R. N. Gedye, F. E.Smith, K. C.Westaway, Tetrahedron Lett, 27, (1986) 279.
    [71] Loupy A. et al., Synthesis, (1998) 1213.
    [72] R. N. Gedye, F. E. Smith, K. C. Westaway, Can. J. Chem., 66 (1988) 17.
    [73] A. K. Bose, M. S. Manhas, M. Ghosh, Heterocycles, 30 (1990) 741.
    [74] W. C. Sun, P. M. Guy, J. H. Jahngen, J. Org. Chem., 53 (1988) 4414.
    [75] D. A. Lewis, J. S. Summers, T. C. Ward, J. Polym. Sci., Part A, Polym. Chem., 30 (1992) 1647.
    [76] C. Shibata, T. Kashima, K. Ohuchi, Jpn. J. Appl. Phys., 35 (1996) 316.
    [77] S. D. Dollington, G. Bond, R. B. Moyes, J. Org. Chem., 56 (1991) 1313.
    [78] I. Plazl, Acta Chim. Slov., 41 (1994) 437.
    [79] K. D. Raner, C. R. Strauss, J. Org. Chem., 57 (1992) 6231.
    [80] R. S. Chriscopher, W. T. Robert, Aust. J. Chem.,41 (2008)286.
    [81] S. K. Lau, F. Zaccardo, M. Little, J. Chromatogr. A 809 (1998) 203.

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