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研究生: 黃靖雅
Huang, Ching-Ya
論文名稱: Lunasin對C2C12肌小管細胞其發炎反應與葡萄糖利用之探討
The effects of lunasin on inflammatory response and glucose utilization in C2C12 myotubes
指導教授: 謝佳倩
Hsieh, Chia-Chien
口試委員: 許珊菁 吳啟豪 謝佳倩
口試日期: 2021/07/14
學位類別: 碩士
Master
系所名稱: 營養科學碩士學位學程
Graduate Program of Nutrition Science
論文出版年: 2021
畢業學年度: 109
語文別: 中文
論文頁數: 88
中文關鍵詞: LunasinC2C12肥胖抗發炎胰島素阻抗
英文關鍵詞: Lunasin, C2C12, obesity, Anti-inflammatory, insulin resistance
研究方法: 實驗設計法
DOI URL: http://doi.org/10.6345/NTNU202101379
論文種類: 學術論文
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  • 第一章 研究動機與目的 1 第二章 文獻探討 2 第一節、肥胖 2 一、定義 2 二、流行病學調查 2 三、肥胖與衍生疾病 3 四、肥胖與代謝症候群 4 五、肥胖與發炎 5 六、肥胖與胰島素阻抗 10 第二節、Lunasin 15 一、簡介 15 二、Lunasin功能介紹 16 第三節、研究方法 20 一、C2C12小鼠肌肉纖維母細胞 20 二、3T3-L1小鼠纖維母細胞 21 三、棕櫚酸誘發胰島素阻抗模式 22 第四節、研究架構 23 第三章 材料與方法 24 第一節、實驗設備及耗材 24 一、實驗儀器設備 24 二、拋棄式無菌耗材 24 第二節、實驗材料與方法 25 一、Lunasin處理對C2C12小鼠肌肉纖維母細胞之細胞存活率影響 25 二、肥胖模式下,Lunasin處理對C2C12小鼠肌小管細胞發炎介質的影響 30 三、肥胖模式下,Lunasin處理對C2C12小鼠肌小管細胞對葡萄糖攝入以及胰島素敏感性的影響 36 四、統計分析 39 第四章 實驗結果 40 第一節、Lunasin處理對C2C12肌肉纖維母細胞之細胞存活率影響 40 一、Lunasin處理對C2C12肌肉纖維母細胞和肌小管細胞存活率影響 40 二、Lunasin處理對C2C12肌小管細胞的攝入情形 42 三、PA處理對C2C12肌小管細胞之細胞存活率影響 44 四、Ad-CM處理對C2C12肌小管細胞之細胞存活率影響 45 第二節、肥胖模式下,lunasin處理對C2C12肌小管細胞發炎介質分泌影響 46 一、Lunasin處理對C2C12肌小管細胞對發炎性基因之影響 46 二、Lunasin處理對C2C12肌小管細胞對發炎性細胞激素之影響 48 三、Lunasin處理對C2C12肌小管細胞對細胞內氧化壓力之影響 51 四、Lunasin處理對C2C12肌小管細胞對細胞活性之影響 54 第三節、肥胖模式下,Lunasin處理對C2C12肌小管細胞葡萄糖攝取影響 58 一、Lunasin處理C2C12肌小管細胞對2-NBDG葡萄糖攝取之影響 58 二、Lunasin處理對C2C12肌小管細胞胰島素敏感性之影響 61 三、Lunasin處理對C2C12肌小管細胞肝醣貯存之影響 64 四、Lunasin處理對C2C12肌小管細胞GLUT4表現之影響 65 第五章 討論 68 一、Lunasin處理對C2C12肌小管細胞對發炎性細胞激素之影響 68 二、Lunasin處理對C2C12肌小管細胞細胞內氧化壓力與細胞活性之影響 69 三、Lunasin處理對C2C12肌小管細胞葡萄糖代謝及胰島素敏感性之影響 70 四、Lunasin處理對C2C12肌小管細胞肝醣之影響 72 第六章 結論 74 附錄 75 參考文獻 77

    世界衛生組織(WHO),Health topics Obesity
    https://www.who.int/topics/obesity/zh/
    衛生福利部國民健康署,成人健康體位標準
    https://www.hpa.gov.tw/Pages/Detail.aspx?nodeid=542&pid=9737
    中華民國糖尿病學會,臺灣糖尿病年鑑2019第2型糖尿病
    http://www.endo- dm.org.tw/dia/direct/index.asp?BK_KIND=36&current=%E8%87%BA%E7%81%A3%E7%B3%96%E5%B0%BF%E7%97%85%E5%B9%B4%E9%91%912019%E7%AC%AC2%E5%9E%8B%E7%B3%96%E5%B0%BF%E7%97%85+++++++++++++
    衛生福利部國民健康署,國民營養健康狀況變遷調查成果報告 2013-2016年
    https://www.hpa.gov.tw/Pages/Detail.aspx?nodeid=3999&pid=11145
    https://www.hpa.gov.tw/Pages/Detail.aspx?nodeid=1405&pid=8840
    周美佳 (2016)。探討種子胜肽Lunasin對於肥胖引起的發炎模式之免疫調節作用 (未出版碩士論文)。國立臺灣師範大學,台北市。
    林品妤 (2019)。Lunasin處理對餵食高脂飲食之C57BL/6小鼠代謝異常之影響 (未出版碩士論文)。國立臺灣師範大學,台北市。
    Al-Sulaiti, H., Diboun, I., Agha, M. V., Mohamed, F. et al. (2019). Metabolic signature of obesity-associated insulin resistance and type 2 diabetes. Journal of translational medicine, 17(1), 348.
    Braakhuis, A. J., Campion, P., & Bishop, K. S. (2016). Reducing Breast Cancer Recurrence: The Role of Dietary Polyphenolics. Nutrients, 8(9), 547.
    Bhaskaran, K., Douglas, I., Forbes, H., dos-Santos-Silva, I., Leon, D. A. et al. (2014). Body-mass index and risk of 22 specific cancers: a population-based cohort study of 5·24 million UK adults. Lancet (London, England), 384(9945), 755–765.
    Bente K. Pedersen & Mark A. Febbraio (2012).Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nature Reviews Endocrinology. 8(8), 457–465.
    Bulló, M., García-Lorda, P., Peinado-Onsurbe, J., Hernández, M., Del Castillo, D. et al. (2002). TNFalpha expression of subcutaneous adipose tissue in obese and morbid obese females: relationship to adipocyte LPL activity and leptin synthesis. International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, 26(5), 652–658.
    Bastard, J. P., Maachi, M., Van Nhieu, J. T., Jardel, C., Bruckert, E. et al. (2002). Adipose tissue IL-6 content correlates with resistance to insulin activation of glucose uptake both in vivo and in vitro. The Journal of clinical endocrinology and metabolism, 87(5), 2084–2089.
    Boyle, J. P., Thompson, T. J., Gregg, E. W., Barker, L. E., & Williamson, D. F. (2010). Projection of the year 2050 burden of diabetes in the US adult population: dynamic modeling of incidence, mortality, and prediabetes prevalence. Population health metrics, 8, 29.
    Bazotte, R. B., Silva, L. G., & Schiavon, F. P. (2014). Insulin resistance in the liver: deficiency or excess of insulin?. Cell cycle (Georgetown, Tex.), 13(16), 2494–2500.
    Bhoomika M Patel, Ramesh K Goyal (2019). Liver and insulin resistance: New wine in old bottle!!! European Journal of Pharmacology, 862, 5,172657
    Boden G, Shulman G (2002). Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and β-cell dysfunction. Eur J Clin Invest, 32:14–23
    Bala, M., Gupta, P., Gupta, S., Dua, A., Injeti, E., & Mittal, A. (2021). Efficient and modified 2-NBDG assay to measure glucose uptake in cultured myotubes. Journal of pharmacological and toxicological methods, 109, 107069.
    Confalonieri, P., Bernasconi, P., Megna, P., Galbiati, S., Cornelio, F., & Mantegazza, R. (2000). Increased expression of beta-chemokines in muscle of patients with inflammatory myopathies. Journal of neuropathology and experimental neurology, 59(2), 164–169.
    Chatterjee, C., Gleddie, S., & Xiao, C. W. (2018). Soybean Bioactive Peptides and Their Functional Properties. Nutrients, 10(9), 1211.
    Calle, E. E., Rodriguez, C., Walker-Thurmond, K., & Thun, M. J. (2003). Overweight, obesity, and mortality from cancer in a prospectively studied cohort of U.S. adults. The New England journal of medicine, 348(17), 1625–1638.
    Czech M. P. (2017). Insulin action and resistance in obesity and type 2 diabetes. Nature medicine, 23(7), 804–814.
    Caroline M Apovian (2016). Obesity: definition, comorbidities, causes, and burden. Am J Manag Care. 22, 7, 176-85.
    Ciaraldi, T. P., Ryan, A. J., Mudaliar, S. R., & Henry, R. R. (2016). Altered Myokine Secretion Is an Intrinsic Property of Skeletal Muscle in Type 2 Diabetes. PloS one, 11(7), e0158209.
    Green, C. J., Pedersen, M., Pedersen, B. K., & Scheele, C. (2011). Elevated NF-κB activation is conserved in human myocytes cultured from obese type 2 diabetic patients and attenuated by AMP-activated protein kinase. Diabetes, 60(11), 2810–2819.
    Cawthorn, W. P., & Sethi, J. K. (2008). TNF-alpha and adipocyte biology. FEBS letters, 582(1), 117–131.
    Circu, M. L., & Aw, T. Y. (2008). Glutathione and apoptosis. Free radical research, 42(8), 689–706.
    Carmichael, R. E., Wilkinson, K. A., & Craig, T. J. (2019). Insulin-dependent GLUT4 trafficking is not regulated by protein SUMOylation in L6 myocytes. Scientific reports, 9(1), 6477.
    Catalán, V., Gómez-Ambrosi, J., Rodríguez, A., Ramírez, B., Silva, C. et al. (2011). Association of increased visfatin/PBEF/NAMPT circulating concentrations and gene expression levels in peripheral blood cells with lipid metabolism and fatty liver in human morbid obesity. Nutrition, metabolism, and cardiovascular diseases : NMCD, 21(4), 245–253.
    Chang, Y. H., Chang, D. M., Lin, K. C., Shin, S. J., & Lee, Y. J. (2011). Visfatin in overweight/obesity, type 2 diabetes mellitus, insulin resistance, metabolic syndrome and cardiovascular diseases: a meta-analysis and systemic review. Diabetes/metabolism research and reviews, 27(6), 515–527.
    Cavazos, A., Morales, E., Dia, V. P., & De Mejia, E. G. (2012). Analysis of lunasin in commercial and pilot plant produced soybean products and an improved method of lunasin purification. Journal of food science, 77(5), C539–C545.
    DeFronzo, R. A., Jacot, E., Jequier, E., Maeder, E., Wahren, J. et al. (1981). The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization. Diabetes, 30(12), 1000–1007.
    Dia, V. P., & Mejia, E. G. (2010). Lunasin promotes apoptosis in human colon cancer cells by mitochondrial pathway activation and induction of nuclear clusterin expression. Cancer letters, 295(1), 44–53.
    DeFronzo, R. A., & Tripathy, D. (2009). Skeletal muscle insulin resistance is the primary defect in type 2 diabetes. Diabetes care, 32 Suppl 2(Suppl 2), S157–S163.
    Dia, V. P., & Gonzalez de Mejia, E. (2011). Lunasin induces apoptosis and modifies the expression of genes associated with extracellular matrix and cell adhesion in human metastatic colon cancer cells. Molecular nutrition & food research, 55(4), 623–634.
    Drori, A., Rotnemer-Golinkin, D., Zolotarov, L., & Ilan, Y. (2017). Oral Administration of CardioAid and Lunasin Alleviates Liver Damage in a High-Fat Diet Nonalcoholic Steatohepatitis Model. Digestion, 96(2), 110–118.
    Delaigle, A. M., Senou, M., Guiot, Y., Many, M. C., & Brichard, S. M. (2006). Induction of adiponectin in skeletal muscle of type 2 diabetic mice: In vivo and in vitro studies. Diabetologia, 49(6), 1311–1323.
    de Luca, C., & Olefsky, J. M. (2008). Inflammation and insulin resistance. FEBS letters, 582(1), 97–105.
    Eruslanov, E., & Kusmartsev, S. (2010). Identification of ROS using oxidized DCFDA and flow-cytometry. Methods in molecular biology (Clifton, N.J.), 594, 57–72.
    Escobar-Morreale, H. F., Botella-Carretero, J. I., Alvarez-Blasco, F., Sancho, J., & San Millán, J. L. (2005). The polycystic ovary syndrome associated with morbid obesity may resolve after weight loss induced by bariatric surgery. The Journal of clinical endocrinology and metabolism, 90(12), 6364–6369.
    Esteghamati, A., Alamdari, A., Zandieh, A., Elahi, S., Khalilzadeh, O. et al. (2011). Serum visfatin is associated with type 2 diabetes mellitus independent of insulin resistance and obesity. Diabetes research and clinical practice, 91(2), 154–158.
    Eckardt, K., Görgens, S. W., Raschke, S., & Eckel, J. (2014). Myokines in insulin resistance and type 2 diabetes. Diabetologia, 57(6), 1087–1099.
    Feng, J., Chen, Q., Yu, F., Wang, Z., Chen, S. et al., (2016). Body Mass Index and Risk of Rheumatoid Arthritis: A Meta-Analysis of Observational Studies. Medicine, 95(8), e2859.
    Fernández-Tomé, S., & Hernández-Ledesma, B. (2019). Current state of art after twenty years of the discovery of bioactive peptide lunasin. Food research international (Ottawa, Ont.), 116, 71–78.
    Fang, P., Yu, M., Zhang, L., Wan, D., Shi, M. et al. (2017). Baicalin against obesity and insulin resistance through activation of AKT/AS160/GLUT4 pathway. Molecular and cellular endocrinology, 448, 77–86.
    Fernández-Tomé, S., Xu, F., Han, Y., Hernández-Ledesma, B., & Xiao, H. (2020). Inhibitory Effects of Peptide Lunasin in Colorectal Cancer HCT-116 Cells and Their Tumorsphere-Derived Subpopulation. International journal of molecular sciences, 21(2), 537.
    Gleeson M. (2000). Interleukins and exercise. The Journal of physiology, 529 Pt 1(Pt 1), 1.
    Guaraldi, F., & Salvatori, R. (2012). Cushing syndrome: maybe not so uncommon of an endocrine disease. Journal of the American Board of Family Medicine : JABFM, 25(2), 199–208.
    Galvez, A. F., & de Lumen, B. O. (1999). A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nature biotechnology, 17(5), 495–500.
    Gortan Cappellari, G., Zanetti, M., Semolic, A., Vinci, P., Ruozi, G., et al. (2016). Unacylated Ghrelin Reduces Skeletal Muscle Reactive Oxygen Species Generation and Inflammation and Prevents High-Fat Diet-Induced Hyperglycemia and Whole-Body Insulin Resistance in Rodents. Diabetes, 65(4), 874–886.
    Giustarini, D., Dalle-Donne, I., Milzani, A., Fanti, P., & Rossi, R. (2013). Analysis of GSH and GSSG after derivatization with N-ethylmaleimide. Nature protocols, 8(9), 1660–1669.Gabbouj, S., Ryhänen, S., Marttinen, M., Wittrahm, R., Takalo, M. et al. (2019). Altered Insulin Signaling in Alzheimer's Disease Brain - Special Emphasis on PI3K-Akt Pathway. Frontiers in neuroscience, 13, 629.
    Guo, X., Sun, W., Luo, G., Wu, L., Xu, G., et al. (2019). Panax notoginseng saponins alleviate skeletal muscle insulin resistance by regulating the IRS1-PI3K-AKT signaling pathway and GLUT4 expression. FEBS open bio, 9(5), 1008–1019.
    Hao, Y., Fan, X., Guo, H., Yao, Y., Ren, G., et al. (2020). Overexpression of the bioactive lunasin peptide in soybean and evaluation of its anti-inflammatory and anti-cancer activities in vitro. Journal of bioscience and bioengineering, 129(4), 395–404.
    Hsieh, C. C., Martínez-Villaluenga, C., de Lumen, B. O., & Hernández-Ledesma, B. (2018). Updating the research on the chemopreventive and therapeutic role of the peptide lunasin. Journal of the science of food and agriculture, 98(6), 2070–2079.
    Hsieh, C. C., Chou, M. J., & Wang, C. H. (2017). Lunasin attenuates obesity-related inflammation in RAW264.7 cells and 3T3-L1 adipocytes by inhibiting inflammatory cytokine production. PloS one, 12(2), e0171969.
    Huang, X., Liu, G., Guo, J., & Su, Z. (2018). The PI3K/AKT pathway in obesity and type 2 diabetes. International journal of biological sciences, 14(11), 1483–1496.
    Hernández-Ledesma, B., & Hsieh, C. C. (2017). Chemopreventive role of foodderived proteins and peptides: A review. Critical reviews in food science and nutrition, 57(11), 2358–2376.
    Hajiaghaalipour, F., Khalilpourfarshbafi, M. & Arya, A. (2015). Modulation of Glucose Transporter Protein by Dietary Flavonoids in Type 2 Diabetes Mellitus. International Journal of Biological Sciences, 11(115):508-524
    Hagman, E., Besor, O., Hershkop, K., Santoro, N., Pierpont, B. et al. (2019). Relation of the degree of obesity in childhood to adipose tissue insulin resistance. Acta diabetologica, 56(2), 219–226.
    Im, K., Mareninov, S., Diaz, M., & Yong, W. H. (2019). An Introduction to Performing Immunofluorescence Staining. Methods in molecular biology (Clifton, N.J.), 1897, 299–311.
    Jia, S., Zhang, S., Yuan, H., & Chen, N. (2015). Lunasin inhibits cell proliferation via apoptosis and reduces the production of proinflammatory cytokines in cultured rheumatoid arthritis synovial fibroblasts. BioMed research international, 2015, 346839.
    Jiang, Q., Pan, Y., Cheng, Y., Li, H., Liu, D., et al. (2016). Lunasin suppresses the migration and invasion of breast cancer cells by inhibiting matrix metalloproteinase-2/-9 via the FAK/Akt/ERK and NF-κB signaling pathways. Oncology reports, 36(1), 253–262.
    Jager, J., Grémeaux, T., Cormont, M., Le Marchand-Brustel, Y., & Tanti, J. F. (2007). Interleukin-1beta-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression. Endocrinology, 148(1), 241–251.
    Kang, S. Y., Kim, E., Kang, I., Lee, M., & Lee, Y. (2018). Anti-Diabetic Effects and Anti-Inflammatory Effects of Laminaria japonica and Hizikia fusiforme in Skeletal Muscle: In Vitro and In Vivo Model. Nutrients, 10(4), 491.
    Kowalski, G. M., & Bruce, C. R. (2014). The regulation of glucose metabolism: implications and considerations for the assessment of glucose homeostasis in rodents. American journal of physiology. Endocrinology and metabolism, 307(10), E859–E871.
    Knudsen, J. G., Bertholdt, L., Joensen, E., Lassen, S. B., Hidalgo, J. et al. (2015). Skeletal muscle interleukin-6 regulates metabolic factors in iWAT during HFD and exercise training. Obesity (Silver Spring, Md.), 23(8), 1616–1624.
    Keller C, Steensberg A, Pilegaard H, Osada T, Saltin B, et al.(2001). Transcriptional activation of the IL-6 gene in human contracting skeletal muscle: influence of muscle glycogen content. FASEB J, 15: 2748–2750.
    Kraegen, E. W., Clark, P. W., Jenkins, A. B., Daley, E. A., Chisholm, D. J. et al. (1991). Development of muscle insulin resistance after liver insulin resistance in high-fat-fed rats. Diabetes, 40(11), 1397–1403.
    Kojta, I., Chacińska, M., & Błachnio-Zabielska, A. (2020). Obesity, Bioactive Lipids, and Adipose Tissue Inflammation in Insulin Resistance. Nutrients, 12(5), 1305.
    Kim, J. Y., Lee, H. J., Lee, S. J., Jung, Y. H., Yoo. et al. (2017). Palmitic Acid-BSA enhances Amyloid-β production through GPR40-mediated dual pathways in neuronal cells: Involvement of the Akt/mTOR/HIF-1α and Akt/NF-κB pathways. Scientific reports, 7(1), 4335.
    Luppino, F. S., de Wit, L. M., Bouvy, P. F., Stijnen, T., Cuijpers, P. et al., (2010). Overweight, obesity, and depression: a systematic review and meta-analysis of longitudinal studies. Archives of general psychiatry, 67(3), 220–229.
    Lim, S., Son, K. R., Song, I. C., Park, H. S., Jin, C. J. et al. (2009). Fat in liver/muscle correlates more strongly with insulin sensitivity in rats than abdominal fat. Obesity (Silver Spring, Md.), 17(1), 188–195.
    Laurberg, P., Knudsen, N., Andersen, S., Carlé, A., Pedersen, I. B. et al. (2012). Thyroid function and obesity. European thyroid journal, 1(3), 159–167.
    Lombardi, G., Sanchis-Gomar, F., Perego, S., Sansoni, V., & Banfi, G. (2016). Implications of exercise-induced adipo-myokines in bone metabolism. Endocrine, 54(2), 284–305.
    Lombardi G, Sanchis-Gomar F, Perego S, Sansoni V, Banfi G. Implications of exercise-induced adipo-myokines in bone metabolism. Endocrine. 2016 Nov;54(2):284-305.
    Luo, Z., Zhang, Y., Li, F., He, J., Ding, H. et al. (2009). Resistin induces insulin resistance by both AMPK-dependent and AMPK-independent mechanisms in HepG2 cells. Endocrine, 36(1), 60–69.
    Leto, D., & Saltiel, A. R. (2012). Regulation of glucose transport by insulin: traffic control of GLUT4. Nature reviews. Molecular cell biology, 13(6), 383–396.
    Leonardini, A., Laviola, L., Perrini, S., Natalicchio, A., & Giorgino, F. (2009). Cross-Talk between PPARgamma and Insulin Signaling and Modulation of Insulin Sensitivity. PPAR research, 2009, 818945.
    Lackey, D. E., & Olefsky, J. M. (2016). Regulation of metabolism by the innate immune system. Nature reviews. Endocrinology, 12(1), 15–28.
    Long, Y. C., & Zierath, J. R. (2006). AMP-activated protein kinase signaling in metabolic regulation. The Journal of clinical investigation, 116(7), 1776–1783.
    Lombardi, G., Ziemann, E., & Banfi, G. (2019). Physical Activity and Bone Health: What Is the Role of Immune System? A Narrative Review of the Third Way. Frontiers in endocrinology, 10, 60.
    Liu, M., Wang, L., Li, X., Wu, Y., Yin, F. et al. (2020). Trilobatin ameliorates insulin resistance through IRS-AKT-GLUT4 signaling pathway in C2C12 myotubes and ob/ob mice. Chinese medicine, 15, 110.
    Lehr, S., Hartwig, S., Lamers, D., Famulla, S., Müller, S. et al. (2012). Identification and validation of novel adipokines released from primary human adipocytes. Molecular & cellular proteomics : MCP, 11(1), M111.010504.
    Luo, Z., Zhang, Y., Li, F., He, J., Ding, H., Yan, L., & Cheng, H. (2009). Resistin induces insulin resistance by both AMPK-dependent and AMPK-independent mechanisms in HepG2 cells. Endocrine, 36(1), 60–69.
    McConnell, E. J., Devapatla, B., Yaddanapudi, K., & Davis, K. R. (2015). The soybean-derived peptide lunasin inhibits non-small cell lung cancer cell proliferation by suppressing phosphorylation of the retinoblastoma protein. Oncotarget, 6(7), 4649–4662.
    McGillicuddy, F. C., Chiquoine, E. H., Hinkle, C. C., Kim, R. J., Shah, R. et al. (2009). Interferon gamma attenuates insulin signaling, lipid storage, and differentiation in human adipocytes via activation of the JAK/STAT pathway. The Journal of biological chemistry, 284(46), 31936–31944.
    Montales, M. T., Simmen, R. C., Ferreira, E. S., Neves, V. A., & Simmen, F. A. (2015). Metformin and soybean-derived bioactive molecules attenuate the expansion of stem cell-like epithelial subpopulation and confer apoptotic sensitivity in human colon cancer cells. Genes & nutrition, 10(6), 49.
    Meng, S. J., & Yu, L. J. (2010). Oxidative stress, molecular inflammation and sarcopenia. International journal of molecular sciences, 11(4), 1509–1526.
    Michael, L. F., Wu, Z., Cheatham, R. B., Puigserver, P., Adelmant, G. et al. (2001). Restoration of insulin-sensitive glucose transporter (GLUT4) gene expression in muscle cells by the transcriptional coactivator PGC-1. Proceedings of the National Academy of Sciences of the United States of America, 98(7), 3820–3825.
    Ma, J., Meng, X., Kang, S. Y., Zhang, J., Jung, H. W. et al. (2019). Regulatory effects of the fruit extract of Lycium chinense and its active compound, betaine, on muscle differentiation and mitochondrial biogenesis in C2C12 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 118, 109297.
    Nehlsen-Cannarella, S. L., Fagoaga, O. R., Nieman, D. C., Henson, D. A., Butterworth, D. E. et al. (1997). Carbohydrate and the cytokine response to 2.5 h of running. Journal of applied physiology (Bethesda, Md. : 1985), 82(5), 1662–1667.
    Nicholson, T., Church, C., Baker, D. J., & Jones, S. W. (2018). The role of adipokines in skeletal muscle inflammation and insulin sensitivity. Journal of inflammation (London, England), 15, 9.
    Olefsky J. M. (1990). The insulin receptor. A multifunctional protein. Diabetes, 39(9), 1009–1016.
    O'Rourke, R. W., White, A. E., Metcalf, M. D., Winters, B. R., Diggs, B. S. et al. (2012). Systemic inflammation and insulin sensitivity in obese IFN-γ knockout mice. Metabolism: clinical and experimental, 61(8), 1152–1161.
    Palomer, X., Pizarro-Delgado, J., Barroso, E., & Vázquez-Carrera, M. (2018). Palmitic and Oleic Acid: The Yin and Yang of Fatty Acids in Type 2 Diabetes Mellitus. Trends in endocrinology and metabolism: TEM, 29(3), 178–190.
    Plomgaard P, Penkowa M, Pedersen BK. (2005). Fiber type specific expression of TNF-alpha, IL-6 and IL-18 in human skeletal muscles. Exerc Immunol Rev, 11: 53–63.
    Pedersen, B. K., & Febbraio, M. A. (2008). Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiological reviews, 88(4), 1379–1406.
    Pearce E. N. (2012). Thyroid hormone and obesity. Current opinion in endocrinology, diabetes, and obesity, 19(5), 408–413.
    Pabona, J. M., Dave, B., Su, Y., Montales, M. T., de Lumen, B. O. et al. (2013). The soybean peptide lunasin promotes apoptosis of mammary epithelial cells via induction of tumor suppressor PTEN: similarities and distinct actions from soy isoflavone genistein. Genes & nutrition, 8(1), 79–90.
    Price, S. J., Pangloli, P., & Dia, V. P. (2017). Pepsin-pancreatin hydrolysis reduced the ability of lunasin-enriched material to inhibit activation of the inflammasomes in THP-1 human macrophages. Food & function, 8(12), 4449–4458.
    Poirier, P., Giles, T. D., Bray, G. A., Hong, Y., Stern, J. S. et al. (2006). Obesity and cardiovascular disease: pathophysiology, evaluation, and effect of weight loss: an update of the 1997 American Heart Association Scientific Statement on Obesity and Heart Disease from the Obesity Committee of the Council on Nutrition, Physical Activity, and Metabolism. Circulation, 113(6), 898–918.
    Pandurangan, M., Veerappan, M., & Kim, D. H. (2015). Cytotoxicity of zinc oxide nanoparticles on antioxidant enzyme activities and mRNA expression in the cocultured C2C12 and 3T3-L1 cells. Applied biochemistry and biotechnology, 175(3), 1270–1280.
    Pedersen, B. K., & Febbraio, M. A. (2012). Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nature reviews. Endocrinology, 8(8), 457–465.
    Price, S. J., Pangloli, P., Krishnan, H. B., & Dia, V. P. (2016). Kunitz trypsin inhibitor in addition to Bowman-Birk inhibitor influence stability of lunasin against pepsin-pancreatin hydrolysis. Food research international (Ottawa, Ont.), 90, 205–215.
    Patti, M. E., Butte, A. J., Crunkhorn, S., Cusi, K., Berria, R. et al. (2003). Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: Potential role of PGC1 and NRF1. Proceedings of the National Academy of Sciences of the United States of America, 100(14), 8466–8471.
    Reaven GM (1991). Insulin resistance and compensatory hyperinsulinemia: role in hypertension, dyslipidemia, and coronary heart disease. Am Heart J,121:1283–1288.
    Reyna, S. M., Ghosh, S., Tantiwong, P., Meka, C. S., Eagan, P. et al. (2008). Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjects. Diabetes, 57(10), 2595–2602.
    Reddy P, Lent-Schochet D, Ramakrishnan N, McLaughlin M, Jialal I.(2019). Metabolic syndrome is an inflammatory disorder: A conspiracy between adipose tissue and phagocytes. Clinica Chimica Acta, 496,35-44. Nutrition, Physical Activity, and Metabolism. Circulation. 113(6), 898–918.
    Ren, G., Zhu, Y., Shi, Z., & Li, J. (2017). Detection of lunasin in quinoa (Chenopodium quinoa Willd.) and the in vitro evaluation of its antioxidant and anti-inflammatory activities. Journal of the science of food and agriculture, 97(12), 4110–4116.
    Sadeghi, A., Seyyed Ebrahimi, S. S., Golestani, A., & Meshkani, R. (2017). Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF-κB Pathway in a SIRT1-Independent Mechanism. Journal of cellular biochemistry, 118(9), 2654–2663.
    Stockert, J. C., Horobin, R. W., Colombo, L. L., & Blázquez-Castro, A. (2018). Tetrazolium salts and formazan products in Cell Biology: Viability assessment, fluorescence imaging, and labeling perspectives. Acta histochemica, 120(3), 159–167.
    Shen, S., Liao, Q., Zhang, T., Pan, R., & Lin, L. (2019). Myricanol modulates skeletal muscle-adipose tissue crosstalk to alleviate high-fat diet-induced obesity and insulin resistance. British journal of pharmacology, 176(20), 3983–4001.
    Sadeghi, A., Rostamirad, A., Seyyedebrahimi, S., & Meshkani, R. (2018). Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by egulating JNK/NF-kB pathway and ROS production. Inflammopharmacology, 26(5), 1265–1272.
    Saltiel, A. R., & Kahn, C. R. (2001). Insulin signalling and the regulation of glucose and lipid metabolism. Nature, 414(6865), 799–806.
    Stolarczyk E. (2017). Adipose tissue inflammation in obesity: a metabolic or immune response?. Current opinion in pharmacology, 37, 35–40.
    Steensberg A, Keller C, Starkie RL, Osada T et al. (2002). IL-6 and TNF-alpha expression in, release from, contracting human skeletal muscle. Am J Physiol Endocrinol Metab, 283:1272–1278.
    Sadagurski, M., Norquay, L., Farhang, J., D'Aquino, K., Copps, K. et al. (2010). Human IL6 enhances leptin action in mice. Diabetologia, 53(3), 525–535.
    Shidal, C., Al-Rayyan, N., Yaddanapudi, K., & Davis, K. R. (2016). Lunasin is a novel therapeutic agent for targeting melanoma cancer stem cells. Oncotarget, 7(51), 84128–84141.
    Sell, H., Dietze-Schroeder, D., Kaiser, U., & Eckel, J. (2006). Monocyte chemotactic protein-1 is a potential player in the negative cross-talk between adipose tissue and skeletal muscle. Endocrinology, 147(5), 2458–2467.
    Sinclair, L. V., Barthelemy, C., & Cantrell, D. A. (2020). Single Cell Glucose Uptake Assays: A Cautionary Tale. Immunometabolism, 2(4), e200029.
    Segal, K. R., Landt, M., & Klein, S. (1996). Relationship between insulin sensitivity and plasma leptin concentration in lean and obese men. Diabetes, 45(7), 988–991.
    Samuel, V. T., & Shulman, G. I. (2012). Mechanisms for insulin resistance: common threads and missing links. Cell, 148(5), 852–871.
    Togashi, N., Ura, N., Higashiura, K., Murakami, H., & Shimamoto, K. (2000). The contribution of skeletal muscle tumor necrosis factor-alpha to insulin resistance and hypertension in fructose-fed rats. Journal of hypertension, 18(11), 1605–1610.
    Trayhurn, P., Drevon, C. A., & Eckel, J. (2011). Secreted proteins from adipose tissue and skeletal muscle - adipokines, myokines and adipose/muscle cross-talk. Archives of physiology and biochemistry, 117(2), 47–56.
    Trujillo, M. E., Sullivan, S., Harten, I., Schneider, S. H., Greenberg, A. S. et al. (2004). Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. The Journal of clinical endocrinology and metabolism, 89(11), 5577–5582.
    Terra, X., Auguet, T., Quesada, I., Aguilar, C., Luna, A. M., Hernández, M., Sabench, F., Porras, J. A., Martínez, S., Lucas, A., Pellitero, S., Llutart, J., del Castillo, D., & Richart, C. (2012). Increased levels and adipose tissue expression of visfatin in morbidly obese women: the relationship with pro-inflammatory cytokines. Clinical endocrinology, 77(5), 691–698.
    Thiebaud, D., Jacot, E., DeFronzo, R. A., Maeder, E., Jequier, E. et al. (1982). The effect of graded doses of insulin on total glucose uptake, glucose oxidation, and glucose storage in man. Diabetes, 31(11), 957–963.
    Uysal, K. T., Wiesbrock, S. M., Marino, M. W., & Hotamisligil, G. S. (1997). Protection from obesity-induced insulin resistance in mice lacking TNF-alpha function. Nature, 389(6651), 610–614.
    van Hall, G., Steensberg, A., Sacchetti, M., Fischer, C., Keller, C. et al. (2003). Interleukin-6 stimulates lipolysis and fat oxidation in humans. The Journal of clinical endocrinology and metabolism, 88(7), 3005–3010.
    Watson, R. T., & Pessin, J. E. (2006). Bridging the GAP between insulin signaling and GLUT4 translocation. Trends in biochemical sciences, 31(4), 215–222.
    Wu, H., & Ballantyne, C. M. (2017). Skeletal muscle inflammation and insulin resistance in obesity. The Journal of clinical investigation, 127(1), 43–54.
    Wang, M., Chang, S. Q., Tian, Y. S., Zhang, G. Q., & Qi, J. (2020). Zengye Decoction Ameliorates Insulin Resistance by Promoting Glucose Uptake. Rejuvenation research, 23(5), 367–376.
    Prospective Studies Collaboration, Whitlock, G., Lewington, S., Sherliker, P., Clarke, R. et al. (2009). Body-mass index and cause-specific mortality in 900 000 adults: collaborative analyses of 57 prospective studies. Lancet (London, England), 373(9669), 1083–1096.
    Yaribeygi, H., Farrokhi, F. R., Butler, A. E., & Sahebkar, A. (2019). Insulin resistance: Review of the underlying molecular mechanisms. Journal of cellular physiology, 234(6), 8152–8161.
    Yadav, A., Jyoti, P., Jain, S. K., & Bhattacharjee, J. (2011). Correlation of adiponectin and leptin with insulin resistance: a pilot study in healthy north Indian population. Indian journal of clinical biochemistry : IJCB, 26(2), 193–196.
    Yau, S. W., Henry, B. A., Russo, V. C., McConell, G. K., Clarke, I. J. et al. (2014). Leptin enhances insulin sensitivity by direct and sympathetic nervous system regulation of muscle IGFBP-2 expression: evidence from nonrodent models. Endocrinology, 155(6), 2133–2143.
    Zhang, R., Qin, X., Zhang, T., Li, Q., Zhang, J. et al. (2018). Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. Molecules (Basel, Switzerland), 23(10), 2711.
    Zhu, Y., Song, W., Everaert, N., Shi, Z., Han, T. et al. (2019). Revealing the regional distribution of soybean lunasin content in China and the effects of climate factors by sampling extensively. Journal of the science of food and agriculture, 99(6), 2802–2807.
    Zhang, Q., Kong, X., Yuan, H., Guan, H., Li, Y. et al. (2019). Mangiferin Improved Palmitate-Induced-Insulin Resistance by Promoting Free Fatty Acid Metabolism in HepG2 and C2C12 Cells via PPARα: Mangiferin Improved Insulin Resistance. Journal of diabetes research, 2019, 2052675.
    Zhang, J., Wang, X., Vikash, V., Ye, Q., Wu, D. et al. (2016). ROS and ROS-Mediated Cellular Signaling. Oxidative medicine and cellular longevity, 2016, 4350965.

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