本研究利用先進雷射微細製程技術(Advanced laser micromachining technique)於旋塗控制之石墨烯薄膜(Spin-coating graphene thin film)上，進行微型加熱器(Micro-heater)的製作。本研究藉由檢測其製作之元件電學特性與加熱特性，並利用程式控制與電路設計，以應用在設計與製作之聚合酶連鎖反應(Polymerase chain reaction, PCR)晶片，進行去氧核醣核酸(Deoxyribonucleic acid, DNA)之增幅。

本研究結果發現製備的微型加熱器長度愈短，石墨烯電極通道寬度愈寬則電學特性愈佳，加熱特性也會愈好，即使用較少的能量就可以達到研究預期的溫度。為了在微型加熱器上安置一個小腔體(Chamber)來進行DNA的增生，本研究選用的設計長度為9 mm與寬度為1 mm之微型加熱器，並藉由LabView程式控制，以固態繼電器(Solid state relay, SSR)與設計脈衝寬度調變(Pulse width modulation, PWM)電路，達到單電壓源輸入及多電壓源輸出的控制。本研究可以真實被應用於PCR反應中的三段溫度控制中，其分別可穩定達到90-95 °C、50-55 °C與72-78 °C。透過以上的實驗參數調控，本研究進行DNA進行增生放大實驗，該實驗量測結果皆有放大特徵，並證明本研究製備之石墨烯微型加熱器，將能有機會實際應用在聚合酶連鎖反應晶片產品之設計與製作。

In this study, the graphene-based micro-heater can be fabricated by the advanced laser micromachining and spinning-coating techniques for thin-film device. Based on electrical and thermal characteristics of graphene-based device, the control program and circuit design for developing polymerase chain reaction (PCR) chip can be performed to deoxyribonucleic acid(DNA) amplification.

The results of this study found that the length of the shorter micro-heater and the width of wider graphene-based electrode channel where the heating characteristics indicate their better. That is to use of less energy can achieve the predicted temperature. In order to DNA amplification in the small chamber on the micro-heater device, the study is to use the design of micro-heater (length: 9 mm; width: 1 mm) with the LabView program control, the solid state relays (SSR) and pulse width modulation circuit to achieve the single voltage source input and multi-source voltage output. The study can be applied to practical PCR chip reaction with three step temperature control, including 90-95 °C, 50-55 °C and 72-78 °C. Through the experimental parameters control, this study can be done DNA amplification experiments. The measured results reveal the its amplification condition. It demonstrates that the graphene-based micro-heater device can be performed and will have the opportunity to develop the design and fabrication of practical PCR chip products.

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