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研究生: 黃香萍
Hsiang-Ping Huang
論文名稱: 咖啡因攝取與運動對血液氧化指標與抗氧化酶的影響
The effects of caffeine supplementation and exercise in lipid peroxide and antioxidative enzymes
指導教授: 謝伸裕
Hsieh, Shen-Yu
林嘉志
Lin, Chia-Chih
學位類別: 碩士
Master
系所名稱: 體育學系
Department of Physical Education
論文出版年: 2006
畢業學年度: 94
語文別: 中文
論文頁數: 64
中文關鍵詞: 咖啡因運動抗氧化酶
英文關鍵詞: caffeine, exercise, antioxidative enzymes
論文種類: 學術論文
相關次數: 點閱:263下載:34
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  • 目的:本研究主要目為探討連續攝取咖啡因二週後,對於中高強度運動刺激造成人體血液過氧化物及抗氧化酶的影響。方法:受試者為13位健康大學男性(年齡:23 ± 0.73 yr;身高:176.5 ± 1.98 cm;體重:77.62 ± 4.21 kg;VO2max:43.39 ± 1.45 ml/kg/min)。實驗採單盲隨機交叉設計,受試者須攝取二週咖啡因 (6 mg/kg)(C期)或安慰劑(P期)。隨後以中高強度原地跑步運動 (85%VO2max) 評估運動前對氧化壓力的變化。在增補前、運動前、運動後分別採集血液樣本分析血漿乳酸濃度、硫巴比妥酸反應物質(TBARS)濃度及紅血球溶胞液的過氧化氫酶(CAT)活性、超氧化離子歧化酶(SOD)活性、麩胱甘肽過氧化酶(GSH-Px)活性。所得數據以相依樣本t考驗、相依樣本雙因子(組別 × 時間)變異數分析( ANOVA )與相依樣本單因子共變數分析 ( ANCOVA ) 檢定。結果:咖啡因可使SOD活性在運動後(28870.77 ± 6275.57 U/g Hb)顯著高於增補前(14347.69 ± 3550.27 U/g Hb) (p<.05);GSH-Px活性在運動後(1.13 ± 0.57 μmol/min/g Hb)亦顯著高於運動前(0.52 ± 0.08 μmol/min/g Hb) (p<.05);CAT活性則在運動後稍微高於運動前,惟未達統計水準。C期與P期乳酸濃度在運動後皆顯著高於運動前 ( p<.05 )。TBARS濃度在C期與P期間或同期各時間點間皆未達顯著差異(p >.05)。結論: 兩週攝取咖啡因可提升中高強度運動後抗氧化酶的能力,但無法減少因氧化壓力所引起的血液脂質過氧化物。

    關鍵詞:咖啡因、運動、抗氧化酶

    Abstract
    Purpose: To investigate the effects of consecutive two-week supplementation of caffeine on blood lipid peroxide and antioxidative enzymes with a stimulus of medium to high intensity of exercise. Methods: Subjects were thirteen healthy college male volunteers (age: 23 ± 0.73 yr, hight: 176.5 ± 1.98 cm, weight: 77.62 ± 4.21 kg, VO2max: 43.39 ± 1.45 ml/kg/min). Either two weeks of caffeine (6mg/kg) (C phase) or placebo (P phase) was orally ingested in a cross-over, single-blind random fashion. At the end of each treatments, a bout of high intensive treadmill exercise (85%VO2max) was performed to observe changes of blood indicators on oxidative stress. Blood samples were collected before, after supplementation ( or before exercise ) and immediately after exercise, from which catalase (CAT) activity, superoxide dismutase (SOD) acivity and glutathione peroxidase(GSH-Px)activity in erythrocyte lysate and plasma thiobarbituric acid reactive substances(TBARS) were analyzed. Paired t test, two-way (treatment × time) ANOVA and one way ANCOVA were used for statistical analysis. Results: SOD activity was significantly increased after exercise (28870.77 ± 6275.57 U/g Hb) than before (14347.69 ± 3550.27 U/g Hb) supplementation in caffeine trial (p<.05). GSH-Px activity was also significantly higher after exercise (1.13 ± 0.57 μmol/min/g Hb) than before (0.52 ± 0.08 μmol/min/g Hb) in caffeine trial (p<.05). The increase in CAT activity was slightly higher after exercise than before in caffeine trial. Lactate was significantly increased after exercise than before (p<.05). However, plasma TBARS showed no significant differences both in treatment trials and time trials. Conclusion: Two-week caffeine supplementation leads to increased activity of blood antioxidative enzymes against oxidative stress of high-intensity exercise, though no changes in lipid peroxide was observed.

    Keywords: caffeine, exercise, antioxidative enzymes

    碩士論文通過簽名書………………………………………………...... i 碩士論文授權書……………………………………………………...... ii 中文摘要..…………………………………………………………........ iii 英文摘要..…………………………………………………………........ iv 謝誌.…………………………………………………………………..... v 目次.…………………………………………………………………..... vi 表次.……………………………………………………………............. ix 圖次.…………………………………………………………………..... x 第壹章 緒論 一、研究背景…………………………………………….………..... 1 二、研究問題……………………………………………………...... 3 三、研究目的……………………………………………………...... 3 四、研究假設……………………………………………………...... 4 五、名詞操作性定義………………………...………..…………….. 4 六、研究限制……………………...………..…………….....……… 4 第貳章 文獻探討 一、自由基與氧化壓力…………………….....………………......... 5 二、運動與氧化性壓力的關係……………………………….......... 6 三、咖啡因與抗氧化……………………………………………...... 7 (一)咖啡因簡介…………………………………………………... 8 (二)咖啡因與抗氧化的關係……………………………………... 11 (三)咖啡因與運動的關係………………………………………... 12 四、本章總結………………………………………………………... 15 第參章 研究方法與步驟 一、受試者……..………………………..………………………….. 17 二、實驗設計……………………………………………………….. 17 三、實驗流程……………………………………………………….. 20 四、血液樣本分析……………………....………………………….. 21 五、統計分析……………………………………………………….. 22 第肆章 結果 一、受試者基本資料……………………….....……………………. 23 二、實驗期間能量消耗之比較………………………….…………. 23 三、單次中高強度運動之持續時間的比較……………………….. 24 四、抗氧化酶活性…………………….……………………………. 24 (一)過氧化氫酶………………………………………….………. 24 (二)超氧化離子歧化酶…………………………………….……. 25 (三)麩胱甘肽過氧化酶………………………………….………. 26 (四)脂質過氧化物—硫巴比妥酸反應物質…….…………......... 26 五、乳酸……....…………………………………….………………. 27 第伍章 討論與結論 一、實驗控制……………………………………………………….. 28 二、咖啡因與運動持續時間、血漿乳酸的影響實........…..…..….. 29 三、攝取咖啡因與運動對抗氧化酶活性的影響………………….. 31 四、攝取咖啡因對於血漿脂質過氧化物—TBARS的影響…….… 32 五、咖啡因與其它物質合併的增補效應.......................................... 34 六、結論.....…………………………………………………………. 35 引用文獻 一、中文部份……………………………………....……………….. 36 二、英文部份…………………………….……………..……........... 37 附錄一 受試者須知及同意書…………………..…………..……….. 42 附錄二 受試者健康情況調查表……………………………..……… 43 附錄三 飲食紀錄表…………………………………………..……… 44 附錄四 身體活動量表……………………………………………..… 45 附錄五 血液生化分析…………………………………………..….... 48 個人小傳…………………………………………..…............................ 54

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