簡易檢索 / 詳目顯示

研究生: 黃香萍
Hsiang-Ping Huang
論文名稱: 咖啡因攝取與運動對血液氧化指標與抗氧化酶的影響
The effects of caffeine supplementation and exercise in lipid peroxide and antioxidative enzymes
指導教授: 謝伸裕
Hsieh, Shen-Yu
林嘉志
Lin, Chia-Chih
學位類別: 碩士
Master
系所名稱: 體育學系
Department of Physical Education
論文出版年: 2006
畢業學年度: 94
語文別: 中文
論文頁數: 64
中文關鍵詞: 咖啡因運動抗氧化酶
英文關鍵詞: caffeine, exercise, antioxidative enzymes
論文種類: 學術論文
相關次數: 點閱:235下載:34
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 目的:本研究主要目為探討連續攝取咖啡因二週後,對於中高強度運動刺激造成人體血液過氧化物及抗氧化酶的影響。方法:受試者為13位健康大學男性(年齡:23 ± 0.73 yr;身高:176.5 ± 1.98 cm;體重:77.62 ± 4.21 kg;VO2max:43.39 ± 1.45 ml/kg/min)。實驗採單盲隨機交叉設計,受試者須攝取二週咖啡因 (6 mg/kg)(C期)或安慰劑(P期)。隨後以中高強度原地跑步運動 (85%VO2max) 評估運動前對氧化壓力的變化。在增補前、運動前、運動後分別採集血液樣本分析血漿乳酸濃度、硫巴比妥酸反應物質(TBARS)濃度及紅血球溶胞液的過氧化氫酶(CAT)活性、超氧化離子歧化酶(SOD)活性、麩胱甘肽過氧化酶(GSH-Px)活性。所得數據以相依樣本t考驗、相依樣本雙因子(組別 × 時間)變異數分析( ANOVA )與相依樣本單因子共變數分析 ( ANCOVA ) 檢定。結果:咖啡因可使SOD活性在運動後(28870.77 ± 6275.57 U/g Hb)顯著高於增補前(14347.69 ± 3550.27 U/g Hb) (p<.05);GSH-Px活性在運動後(1.13 ± 0.57 μmol/min/g Hb)亦顯著高於運動前(0.52 ± 0.08 μmol/min/g Hb) (p<.05);CAT活性則在運動後稍微高於運動前,惟未達統計水準。C期與P期乳酸濃度在運動後皆顯著高於運動前 ( p<.05 )。TBARS濃度在C期與P期間或同期各時間點間皆未達顯著差異(p >.05)。結論: 兩週攝取咖啡因可提升中高強度運動後抗氧化酶的能力,但無法減少因氧化壓力所引起的血液脂質過氧化物。

    關鍵詞:咖啡因、運動、抗氧化酶

    Abstract
    Purpose: To investigate the effects of consecutive two-week supplementation of caffeine on blood lipid peroxide and antioxidative enzymes with a stimulus of medium to high intensity of exercise. Methods: Subjects were thirteen healthy college male volunteers (age: 23 ± 0.73 yr, hight: 176.5 ± 1.98 cm, weight: 77.62 ± 4.21 kg, VO2max: 43.39 ± 1.45 ml/kg/min). Either two weeks of caffeine (6mg/kg) (C phase) or placebo (P phase) was orally ingested in a cross-over, single-blind random fashion. At the end of each treatments, a bout of high intensive treadmill exercise (85%VO2max) was performed to observe changes of blood indicators on oxidative stress. Blood samples were collected before, after supplementation ( or before exercise ) and immediately after exercise, from which catalase (CAT) activity, superoxide dismutase (SOD) acivity and glutathione peroxidase(GSH-Px)activity in erythrocyte lysate and plasma thiobarbituric acid reactive substances(TBARS) were analyzed. Paired t test, two-way (treatment × time) ANOVA and one way ANCOVA were used for statistical analysis. Results: SOD activity was significantly increased after exercise (28870.77 ± 6275.57 U/g Hb) than before (14347.69 ± 3550.27 U/g Hb) supplementation in caffeine trial (p<.05). GSH-Px activity was also significantly higher after exercise (1.13 ± 0.57 μmol/min/g Hb) than before (0.52 ± 0.08 μmol/min/g Hb) in caffeine trial (p<.05). The increase in CAT activity was slightly higher after exercise than before in caffeine trial. Lactate was significantly increased after exercise than before (p<.05). However, plasma TBARS showed no significant differences both in treatment trials and time trials. Conclusion: Two-week caffeine supplementation leads to increased activity of blood antioxidative enzymes against oxidative stress of high-intensity exercise, though no changes in lipid peroxide was observed.

    Keywords: caffeine, exercise, antioxidative enzymes

    碩士論文通過簽名書………………………………………………...... i 碩士論文授權書……………………………………………………...... ii 中文摘要..…………………………………………………………........ iii 英文摘要..…………………………………………………………........ iv 謝誌.…………………………………………………………………..... v 目次.…………………………………………………………………..... vi 表次.……………………………………………………………............. ix 圖次.…………………………………………………………………..... x 第壹章 緒論 一、研究背景…………………………………………….………..... 1 二、研究問題……………………………………………………...... 3 三、研究目的……………………………………………………...... 3 四、研究假設……………………………………………………...... 4 五、名詞操作性定義………………………...………..…………….. 4 六、研究限制……………………...………..…………….....……… 4 第貳章 文獻探討 一、自由基與氧化壓力…………………….....………………......... 5 二、運動與氧化性壓力的關係……………………………….......... 6 三、咖啡因與抗氧化……………………………………………...... 7 (一)咖啡因簡介…………………………………………………... 8 (二)咖啡因與抗氧化的關係……………………………………... 11 (三)咖啡因與運動的關係………………………………………... 12 四、本章總結………………………………………………………... 15 第參章 研究方法與步驟 一、受試者……..………………………..………………………….. 17 二、實驗設計……………………………………………………….. 17 三、實驗流程……………………………………………………….. 20 四、血液樣本分析……………………....………………………….. 21 五、統計分析……………………………………………………….. 22 第肆章 結果 一、受試者基本資料……………………….....……………………. 23 二、實驗期間能量消耗之比較………………………….…………. 23 三、單次中高強度運動之持續時間的比較……………………….. 24 四、抗氧化酶活性…………………….……………………………. 24 (一)過氧化氫酶………………………………………….………. 24 (二)超氧化離子歧化酶…………………………………….……. 25 (三)麩胱甘肽過氧化酶………………………………….………. 26 (四)脂質過氧化物—硫巴比妥酸反應物質…….…………......... 26 五、乳酸……....…………………………………….………………. 27 第伍章 討論與結論 一、實驗控制……………………………………………………….. 28 二、咖啡因與運動持續時間、血漿乳酸的影響實........…..…..….. 29 三、攝取咖啡因與運動對抗氧化酶活性的影響………………….. 31 四、攝取咖啡因對於血漿脂質過氧化物—TBARS的影響…….… 32 五、咖啡因與其它物質合併的增補效應.......................................... 34 六、結論.....…………………………………………………………. 35 引用文獻 一、中文部份……………………………………....……………….. 36 二、英文部份…………………………….……………..……........... 37 附錄一 受試者須知及同意書…………………..…………..……….. 42 附錄二 受試者健康情況調查表……………………………..……… 43 附錄三 飲食紀錄表…………………………………………..……… 44 附錄四 身體活動量表……………………………………………..… 45 附錄五 血液生化分析…………………………………………..….... 48 個人小傳…………………………………………..…............................ 54

    一、中文部分
    吳慶瑞(1994)。持續運動前攝取咖啡對運動前後人體中血脂及血脂蛋白之影響。體育學報,17,391-410。
    吳慶瑞、王朝鐘、鄭清榮、徐成金、原素娟(1995)。運動前攝取咖啡因對脂肪代謝效率及運動耐力表現之影響。大專體育,23,138-146。
    呂昌明、林旭龍、黃奕清、李明憲、王淑芳 (2000)。身體活動自我報告量表之效度及信度的研究—以Polar Vantage NV心博率監測器為效標。衛生教育學報,14,33-48。
    林文煌(1996)。咖啡因與運動能力。中華體育,10 (3),130-140。
    林天送(1996)。你的生命活力-從自由基談起。台北市:吳氏圖書股份有限公司。
    林貴福、徐台閣、吳慧君(譯)(2003)。運動生理學。台北市:藝軒。( Powers, S. K., & Howley, E. T., 2001 )
    吳家慶(2005)。不同強度動態恢復對損傷肌肉之能及跑步經濟性的影響。未出版博士論文,國立台灣師範大學,台北市。
    姚承義 (2002)。抗氧化劑增補對激烈運動及魚油引發之氧化壓力的影響(博士論文,國立臺灣師範大學,2000)。全國博碩士論文資訊網,90NTNU0567005。
    姚承義 (2005)。魚油增補對運動引發氧化壓力的探討。運動生理暨體能學報,2,67-78。
    馮連世、楊逵生、宗坯方、郭軍(1994)。急性運動對血漿超氧化物歧化酵素的影響及其與有氧能力的關係。中國運動醫學雜誌,13 ( 3 ),129-132。

    謝伸裕(1988)。咖啡因和冷刺激對運動代謝和有氧工作效率的影響。師大學報,33,1-5。
    謝錦成(1997)。耐力運動對人體骨骼肌抗氧化酶的影響。體育學報,22,237-248。
    廖家祺、許美智、劉珍芳(2001)。中強度有氧舞蹈訓練對身體組成及脂質過氧化物質的影響。大專體育學刊,3(1),55-63。
    廖威章(2000)。衰竭運動對老鼠肺臟、心臟、肝臟及腎臟脂質過氧化物的影響。大專體育學刊,2(1),109-118。
    二、 英文部分
    Ashton, T., Rowlands, C. C., Jones, E., Young, I. S., Jackson, S. K., Davies, B., et al. (1998). Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise. European Journal of Applied Physiology and Occupational Physiology, 77(6), 498-502.

    Collomp, K., Ahmaidi, S., Audran, M., Chanal, J. L., & Prefaut, C. (1991). Effects of caffeine ingestion on performance and anaerobic metabolism during the Wingate Test. International Journal of Sports Medicine, 12(5), 439-443.

    Collomp, K., Ahmaidi, S., Chatard, J. C., Audran, M., & Prefaut, C. (1992). Benefits of caffeine ingestion on sprint performance in trained and untrained swimmers. European Journal of Applied Physiology and Occupational Physiology, 64(4), 377-380.

    Collomp, K., Caillaud, C., Audran, M., Chanal, J. L., & Prefaut, C. (1990). [Effect of acute or chronic administration of caffeine on performance and on catecholamines during maximal cycle ergometer exercise]. Comptes Rendus des Séances de la Société de Biologie et de ses Filiales, 184(1), 87-92.

    Davies, K. J., Quintanilha, A. T., Brooks, G. A., & Packer, L. (1982). Free radicals and tissue damage produced by exercise. Biochemical and Biophysical Research Communication, 107(4), 1198-1205.

    Dews, P.B. (1984). Behavioral effects of caffeine. In: P. B. Dews ( Eds ), Caffeine: Perspective from recent research, Berlin Heiblbug : Springer Verlag, 86-103.

    Devasagayam, T. P., Kamat, J. P., Mohan, H., & Kesavan, P. C. (1996). Caffeine as an antioxidant: inhibition of lipid peroxidation induced by reactive oxygen species. Biochimica et Biophysica Acta, 1282(1), 63-70.

    Dodd, S. L., Herb, R. A., & Powers, S. K. (1993). Caffeine and exercise performance. An update. Sport Medicine, 15(1), 14-23.

    Doherty, M., Smith, P. M., Davison, R. C., & Hughes, M. G. (2002). Caffeine is ergogenic after supplementation of oral creatine monohydrate. Medicine and Science in Sports and Exercise, 34(11), 1785-1792.

    Doubt, T. J., & Hsieh, S. S. (1991). Additive effects of caffeine and cold water during submaximal leg exercise. Medicine and Science in Sports and Exercise, 23(4), 435-442.

    Fernandez, M. J., Lopez, A., & Santa-Maria, A. (2003). Apoptosis induced by different doses of caffeine on Chinese hamster ovary cells. J Journal of Applied Toxicology, 23(4), 221-224.

    Fiebich, B. L., Candelario-Jalil, E., Mantovani, M., Heinzmann, M., Akundi, R. S., Hull, M., et al. (2006). Modulation of catecholamine release from rat striatal slices by the fixed combination of aspirin, paracetamol and caffeine. Pharmacological research : the official journal of the Italian Pharmacological Society, 53(4), 391-396.

    Graham, T. E., Helge, J. W., MacLean, D. A., Kiens, B., & Richter, E. A. (2000). Caffeine ingestion does not alter carbohydrate or fat metabolism in human skeletal muscle during exercise. The Journal of physiology, 529 Pt 3, 837-847.

    Hodgson, J. M., Croft, K. D., Mori, T. A., Burke, V., Beilin, L. J., & Puddey, I. B. (2002). Regular ingestion of tea does not inhibit in vivo lipid peroxidation in humans. The Journal of Nutrition, 132(1), 55-58.

    Juhn, M. S. (2002). Ergogenic aids in aerobic activity. Current sports medicine reports, 1(4), 233-238.

    Kanter, M. M., Lesmes, G. R., Kaminsky, L. A., La Ham-Saeger, J., & Nequin, N. D. (1988). Serum creatine kinase and lactate dehydrogenase changes following an eighty kilometer race. Relationship to lipid peroxidation. European Journal of Applied Physiology, 57(1), 60-63.

    Krisko, A., Kveder, M., & Pifat, G. (2005). Effect of caffeine on oxidation susceptibility of human plasma low density lipoproteins. Clinica Chimica Acta, 355(1-2), 47-53.

    Kumar, C. T., Reddy, V. K., Prasad, M., Thyagaraju, K., & Reddanna, P. (1992). Dietary supplementation of vitamin E protects heart tissue from exercise-induced oxidant stress. Molecular and Cellular Biochemistry, 111(1-2), 109-115.

    Lee, C. (2000). Antioxidant ability of caffeine and its metabolites based on the study of oxygen radical absorbing capacity and inhibition of LDL peroxidation. Annals of the New York Academy of Sciences, 295(1-2), 141-154.

    Liu, J. F., Chang, W. Y., Chan, K. H., Tsai, W. Y., Lin, C. L., & Hsu, M. C. (2005). Blood Lipid Peroxides and Muscle Damage Increased following Intensive Resistance Training of Female Weightlifters. Annals of the New York Academy of Sciences, 1042, 255-261.

    Lotito, S. B., & Fraga, C. G. (2000). Catechins delay lipid oxidation and alpha-tocopherol and beta-carotene depletion following ascorbate depletion in human plasma. Proceedings of the Society for Experimental Biology and Medicine, 225(1), 32-38.

    Mougios, V., Ring, S., Petridou, A., & Nikolaidis, M. G. (2003). Duration of coffee- and exercise-induced changes in the fatty acid profile of human serum. Journal of Applied Physiology, 94(2), 476-484.

    Mukhopadhyay, S., Mondal, A., & Poddar, M. K. (2003). Chronic administration of caffeine: effect on the activities of hepatic antioxidant enzymes of Ehrlich ascites tumor-bearing mice. Indian Journal of Experimental Biology, 41(4), 283-289.

    Nikolic, J., Bjelakovic, G., & Stojanovic, I. (2003). Effect of caffeine on metabolism of L-arginine in the brain. Molecular and Cellular Biochemistry, 244(1-2), 125-128.

    Pellegrini, N., Serafini, M., Colombi, B., Del Rio, D., Salvatore, S., Bianchi, M., et al. (2003). Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. The Journal of Nutrition, 133(9), 2812-2819.

    Reid, T. R. (2005, January). Caffeine. National Geogeraphic, 5 (1), 2-36

    Ryu, S., Choi, S. K., Joung, S. S., Suh, H., Cha, Y. S., Lee, S., et al. (2001). Caffeine as a lipolytic food component increases endurance performance in rats and athletes. Journal of Nutritional Science and Vitaminology, 47(2), 139-146.

    Spriet, L. L., MacLean, D. A., Dyck, D. J., Hultman, E., Cederblad, G., & Graham, T. E. (1992). Caffeine ingestion and muscle metabolism during prolonged exercise in humans. American Journal Physiology, 262(6 Pt 1), E891-898.

    Urso, M. L., & Clarkson, P. M. (2003). Oxidative stress, exercise, and antioxidant supplementation. Toxicology, 189(1-2), 41-54.

    Van Soeren, M. H., & Graham, T. E. (1998). Effect of caffeine on metabolism, exercise endurance, and catecholamine responses after withdrawal. Journal of Applied Physiology, 85(4), 1493-1501.

    Wu, W. P., Hao, J. X., Fredholm, B. B., Wiesenfeld-Hallin, Z., & Xu, X. J. (2006). Effect of acute and chronic administration of caffeine on pain-like behaviors in rats with partial sciatic nerve injury. Neuroscience letters, 402(1-2), 164-166.

    Young, I. S., & Woodside, J. V. (2001). Antioxidants in health and disease. Journal of Clinical Pathology, 54(3), 176-186.

    Yukawa, G. S., Mune, M., Otani, H., Tone, Y., Liang, X. M., Iwahashi, H., et al. (2004). Effects of coffee consumption on oxidative susceptibility of low-density lipoproteins and serum lipid levels in humans. Biochemistry (Moscow), 69(1), 70-74.

    QR CODE