研究生: |
賴昱龍 |
---|---|
論文名稱: |
CIA2與HAP蛋白之交互作用 |
指導教授: | 孫智雯 |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
論文出版年: | 2009 |
畢業學年度: | 97 |
語文別: | 中文 |
論文頁數: | 42 |
中文關鍵詞: | CIA2 、HAP |
論文種類: | 學術論文 |
相關次數: | 點閱:157 下載:5 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
葉綠體是植物細胞進行光合作用重要的胞器。為了維持葉綠體的正常功能,從細胞核基因合成的葉綠體蛋白必須正確地被葉綠體膜上的轉運蛋白辨識及輸入。我們之前的研究顯示,CIA2蛋白是CCT-class的轉錄因子,可正向調控葉綠體運組基因Toc33和Toc75。本研究發現CIA2的CCT區域和HAP2序列具有相似性,且其保守胺基酸與HAP蛋白質交互作用及DNA結合有關。利用酵母菌雙雜交實驗證實CIA2和HAP5a、HAP5b及HAP5c有交互作用。檢測不同長度CIA2片段蛋白與HAP5進行酵母菌雙雜交實驗,結果顯示其N端序列和C端的CCT motif,可能與蛋白質交互作用有關。比對植物的CIA2同源蛋白發現N端序列和CCT motif皆具高度保守性,顯示CIA2的N端和CCT motif具有重要蛋白質功能。
Altschul, S.F., and Lipman, D.J. (1990). Protein database searches for multiple alignments. Proc Natl Acad Sci U S A 87, 5509-5513.
Ben-Naim, O., Eshed, R., Parnis, A., Teper-Bamnolker, P., Shalit, A., Coupland, G., Samach, A., and Lifschitz, E. (2006). The CCAAT binding factor can mediate interactions between CONSTANS-like proteins and DNA. Plant J 46, 462-476.
Chen, N.Z., Zhang, X.Q., Wei, P.C., Chen, Q.J., Ren, F., Chen, J., and Wang, X.C. (2007). AtHAP3b plays a crucial role in the regulation of flowering time in Arabidopsis during osmotic stress. J Biochem Mol Biol 40, 1083-1089.
Combier, J.P., de Billy, F., Gamas, P., Niebel, A., and Rivas, S. (2008). Trans-regulation of the expression of the transcription factor MtHAP2-1 by a uORF controls root nodule development. Genes Dev 22, 1549-1559.
Hall, B.G., and Salipante, S.J. (2007). Measures of clade confidence do not correlate with accuracy of phylogenetic trees. PLoS Comput Biol 3, e51.
Jarvis, P. (2008). Targeting of nucleus-encoded proteins to chloroplasts in plants. New Phytol 179, 257-285.
Kumimoto, R.W., Adam, L., Hymus, G.J., Repetti, P.P., Reuber, T.L., Marion, C.M., Hempel, F.D., and Ratcliffe, O.J. (2008). The Nuclear Factor Y subunits NF-YB2 and NF-YB3 play additive roles in the promotion of flowering by inductive long-day photoperiods in Arabidopsis. Planta 228, 709-723.
Kwong, R.W., Bui, A.Q., Lee, H., Kwong, L.W., Fischer, R.L., Goldberg, R.B., and Harada, J.J. (2003). LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development. Plant Cell 15, 5-18.
Leister, D. (2003). Chloroplast research in the genomic age. Trends Genet 19, 47-56.
Li, X.Y., Mantovani, R., Hooft van Huijsduijnen, R., Andre, I., Benoist, C., and Mathis, D. (1992). Evolutionary variation of the CCAAT-binding transcription factor NF-Y. Nucleic Acids Res 20, 1087-1091.
Martin, W., Rujan, T., Richly, E., Hansen, A., Cornelsen, S., Lins, T., Leister, D., Stoebe, B., Hasegawa, M., and Penny, D. (2002). Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus. Proc Natl Acad Sci U S A 99, 12246-12251.
Masaki, T., Tsukagoshi, H., Mitsui, N., Nishii, T., Hattori, T., Morikami, A., and Nakamura, K. (2005). Activation tagging of a gene for a protein with novel class of CCT-domain activates expression of a subset of sugar-inducible genes in Arabidopsis thaliana. Plant J 43, 142-152.
Nelson, D.E., Repetti, P.P., Adams, T.R., Creelman, R.A., Wu, J., Warner, D.C., Anstrom, D.C., Bensen, R.J., Castiglioni, P.P., Donnarummo, M.G., Hinchey, B.S., Kumimoto, R.W., Maszle, D.R., Canales, R.D., Krolikowski, K.A., Dotson, S.B., Gutterson, N., Ratcliffe, O.J., and Heard, J.E. (2007). Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci U S A 104, 16450-16455.
Obenauer, J.C., Cantley, L.C., and Yaffe, M.B. (2003a). Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs. Nucleic Acids Res 31, 3635-3641.
Obenauer, S., Hermann, K.P., Marten, K., Luftner-Nagel, S., von Heyden, D., Skaane, P., and Grabbe, E. (2003b). Soft copy versus hard copy reading in digital mammography. J Digit Imaging 16, 341-344.
Olesen, J., Hahn, S., and Guarente, L. (1987). Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner. Cell 51, 953-961.
Olesen, J.T., and Guarente, L. (1990). The HAP2 subunit of yeast CCAAT transcriptional activator contains adjacent domains for subunit association and DNA recognition: model for the HAP2/3/4 complex. Genes Dev 4, 1714-1729.
Putterill, J., Robson, F., Lee, K., Simon, R., and Coupland, G. (1995). The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80, 847-857.
Rosenberg, F.J. (1991). Thermotolerance in the yeast Saccharomyces cerevisiae.
Salome, P.A., To, J.P., Kieber, J.J., and McClung, C.R. (2006). Arabidopsis response regulators ARR3 and ARR4 play cytokinin-independent roles in the control of circadian period. Plant Cell 18, 55-69.
Schnell, D.J., Blobel, G., Keegstra, K., Kessler, F., Ko, K., and Soll, J. (1997). A consensus nomenclature for the protein-import components of the chloroplast envelope. Trends Cell Biol 7, 303-304.
Siefers, N., Dang, K.K., Kumimoto, R.W., Bynum, W.E.t., Tayrose, G., and Holt, B.F., 3rd. (2009). Tissue-specific expression patterns of Arabidopsis NF-Y transcription factors suggest potential for extensive combinatorial complexity. Plant Physiol 149, 625-641.
Strayer, C., Oyama, T., Schultz, T.F., Raman, R., Somers, D.E., Mas, P., Panda, S., Kreps, J.A., and Kay, S.A. (2000). Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog. Science 289, 768-771.
Su, Y.S., and Lagarias, J.C. (2007). Light-independent phytochrome signaling mediated by dominant GAF domain tyrosine mutants of Arabidopsis phytochromes in transgenic plants. Plant Cell 19, 2124-2139.
Sun, C.W., Huang, Y.C., and Chang, H.Y. (2009). CIA2 coordinatedly up-regulates protein import and synthesis in leaf chloroplasts. Plant Physiol.
Sun, C.W., Chen, L.J., Lin, L.C., and Li, H.M. (2001). Leaf-specific upregulation of chloroplast translocon genes by a CCT motif-containing protein, CIA 2. Plant Cell 13, 2053-2061.
Tamura, K., Dudley, J., Nei, M., and Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24, 1596-1599.
Thompson, J.D., Gibson, T.J., and Higgins, D.G. (2002). Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinformatics Chapter 2, Unit 2 3.
Turner, A., Beales, J., Faure, S., Dunford, R.P., and Laurie, D.A. (2005). The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley. Science 310, 1031-1034.
Wallas, T.R., Smith, M.D., Sanchez-Nieto, S., and Schnell, D.J. (2003). The roles of toc34 and toc75 in targeting the toc159 preprotein receptor to chloroplasts. J Biol Chem 278, 44289-44297.
Wenkel, S., Turck, F., Singer, K., Gissot, L., Le Gourrierec, J., Samach, A., and Coupland, G. (2006). CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis. Plant Cell 18, 2971-2984.
Xing, Y., Zhang, S., Olesen, J.T., Rich, A., and Guarente, L. (1994). Subunit interaction in the CCAAT-binding heteromeric complex is mediated by a very short alpha-helix in HAP2. Proc Natl Acad Sci U S A 91, 3009-3013.
Yan, L., Loukoianov, A., Blechl, A., Tranquilli, G., Ramakrishna, W., SanMiguel, P., Bennetzen, J.L., Echenique, V., and Dubcovsky, J. (2004). The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science 303, 1640-1644.
Yano, M., Katayose, Y., Ashikari, M., Yamanouchi, U., Monna, L., Fuse, T., Baba, T., Yamamoto, K., Umehara, Y., Nagamura, Y., and Sasaki, T. (2000). Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12, 2473-2484.
Zobell, O., Coupland, G., and Reiss, B. (2005). The family of CONSTANS-like genes in Physcomitrella patens. Plant Biol (Stuttg) 7, 266-275.