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研究生: Cagayan, Gerald Reymari Acoba
Cagayan, Gerald Reymari Acoba
論文名稱: 以奈米光柵耦合表面電漿子共振增強拉曼散射於生醫感測之應用
Nanograting-coupled Surface Plasmon Resonance (SPR)-enhanced Raman Scattering for biosensing applications
指導教授: 邱南福
Chiu, Nan-Fu
口試委員: 邱南福
Chiu, Nan-Fu
張家禎
Chang, Chia-Chen
陳震宇
Chen, Chen-Yu
口試日期: 2024/03/29
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2024
畢業學年度: 112
語文別: 英文
論文頁數: 73
中文關鍵詞: 表面電漿子共振增拉曼散射奈米光柵耦合生醫感測之
英文關鍵詞: surface plasmon resonance, Raman scattering, grating-coupling, Raman enhancement, biosensing
DOI URL: http://doi.org/10.6345/NTNU202400462
論文種類: 學術論文
相關次數: 點閱:155下載:0
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  • Enormous interest in biosensors has drawn considerable attention among researchers, given its wide applications in clinical treatment, biomedical technology, healthcare, pharmaceuticals, and food science. Specifically, the surface plasmon resonance (SPR) technique has served as a robust biosensor, and its versatile principle makes SPR a widely utilized sensing method extensively employed to study biomolecular interactions, material properties, and chemical processes. In this study, we designed a grating-coupler SPR system integrated into a preconfigured Raman spectrometer system to measure the Raman enhancement on Rhodamine 6G adsorbed on two different structures: linear and circular nanograting. Experiments show that SPR-Raman enhancement is achieved for linear and circular structures by approximately 2.14 × 103 and 2.46 × 103, respectively, when the sample is simultaneously excited by the Raman probe at normal incidence and the SPR system at resonance angle. The propagating SPPs allow the sample to be in an excited state, providing an enhanced evanescent field, which is then coupled with the localized SPPs upon Raman excitation and measurement. These results suggest that SPR can be used to enhance the Raman signal of an analyte and improve detection.

    Acknowledgement i Abstract ii Table of Contents iii List of Figures vii Abbreviations and Symbols x Chapter 1: Introduction 1 1.1 Overview 1 1.2 Thesis Structure 3 1.3 Research objectives 4 References 5 Chapter 2: Theoretical Background 8 2.1 Surface Plasmon Resonance (SPR): Principle 8 2.2 SPPs at a single interface 9 2.2.1 Transverse Magnetic (TM) mode 9 2.2.2 Transverse Electric (TE) mode 11 2.3 Dispersion relation 12 2.4 SPPs excitation methods 14 2.4.1 Prism coupling 14 2.4.2 Grating coupling 15 2.4.3 Localized Surface Plasmon Resonance (LSPR) 17 2.5 SPR performance metrics 18 2.6 Raman Scattering: Principle 19 2.6.1 Coherent Anti-Stokes Raman Scattering (CARS) 21 2.6.2 Surface-enhanced Raman Scattering (SERS) 22 2.6.3 Resonance Raman Scattering (RRS) 23 2.6.4 Plasmon-enhanced Raman Scattering (PERS) 23 References 25 Chapter 3: Experimental Methods 32 3.1 Grating-coupler SPR optical system 32 3.2 Nanostructure and sensing layer design 34 3.2.1 Linear grating 34 3.2.2 Circular grating 35 3.2.3 Sensing layer structure design 36 3.3 Sample protocol, cleaning, and coating methods 37 3.3.1 Rhodamine 6G solution protocol 37 3.3.2 Chip cleaning method 38 3.3.3 Rhodamine 6G coating method 38 3.4 Experimental Procedure 39 References 40 Chapter 4: Results and Discussion 41 4.1 Reflectance Spectra 41 4.1.1 Linear nanograting SPR 41 4.1.2 Concentric ring SPR 44 4.1.3 Rhodamine 6G-coated film SPR 45 4.2 Performance analysis 47 4.2.1 Nanograting structure performance 47 4.2.2 R6G-coated film FWHM and SNR 49 4.3 Raman spectra of R6G 50 4.3.1 Raman spectra on linear nanograting 50 4.3.2 Raman spectra on concentric ring 54 4.4 Enhancement factor 56 References 62 Chapter 5: Conclusion and Recommendations 70 5.1 Conclusion 70 5.2 Recommendations 72 Appendix A Figure A A Figure B B Figure C B Figure D C Figure E D Figure F D Figure G E Figure HF References F

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