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研究生: 楊博年
Po-Nien Yang
論文名稱: 以光學同調斷層攝影術評估果蠅的心臟功能
Assessment of the heart function in adult Drosophila melanogaster by Swept Source Optical Coherence Tomography
指導教授: 郭文娟
Kuo, Wen-Chuan
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2008
畢業學年度: 96
語文別: 中文
論文頁數: 78
中文關鍵詞: 心跳率心臟舒張維度心臟收縮維度心肌短縮分率
英文關鍵詞: Heart Rate, end-diastolic dimension, end-systolic dimension, fractional shortening
論文種類: 學術論文
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  • 本研究提出用三維快速高解析度掃瞄式光學同調斷層儀(SS-OCT)系統以進行非侵入式活體成蟲果蠅心臟造影成像,分別量測正常標準種類之果蠅(Drosophila,w1118),與心臟突變之果蠅(UAS-Acer、Mut8i、Mut9i、M8i、M9i、Δ164F1、Δ168M1)。並利用影像後處理的資訊取得果蠅各項心臟功能參數,如心跳率(Heart Rate,HR)、心臟舒張維度(end-diastolic dimension,EDD)、心臟收縮維度(end-systolic dimension,ESD)以及心肌短縮分率(fractional shortening,FS),本研究目的包括(1)測試此套系統是否適用於清醒的成蟲果蠅之心臟功能精確量測;(2)判定果蠅是否為一個合適的重建人類心臟病的模式動物系統。

    In this study, a three dimensional fast scanning and high resolution Swept Source optical coherence tomography (SS-OCT) system for non-invasively imaging the heart in adult awake Drosophila of a standard strain (w1118) and mutation with cardiac function(UAS-Acer、Mut8i、Mut9i、M8i、M9i、Δ164F1、Δ168M1) is proposed. Heart functions such as Heart Rate (HR), end-diastolic dimension (EDD), end-systolic dimension (ESD), and fractional shortening (FS) were acquired by image post processing. The objectives of this study including: (1) to test whether the SS-OCT system could be applied to accurately measure cardiac function in awake adult Drosophila; (2) to determine whether the fly can be used as a model system to recapitulate human heart diseases.

    目錄 中文摘要-------------------------------------------------------------------------- I 英文摘要--------------------------------------------------------------------------Ⅱ 目錄--------------------------------------------------------------------------------Ⅲ 圖目錄索引--------------------------------------------------------------------- Ⅶ 表目錄索引--------------------------------------------------------------------- XI 第一章 緒論-------------------------------------------------------------------- 1 1.1 研究動機與目的----------------------------------------------------------- 1 1.2 文獻回顧-------------------------------------------------------------------- 3 1.2.1 光學同調斷層攝影術(OCT) ---------------------------------- 3 1.2.2 掃瞄式光源之光學同調斷層攝影術(SS-OCT) ------------ 4 1.2.3 血管收縮素轉換酶(ACE)與血管收縮素相關轉換酶(Acer)------------------------------------------------------------- 5 1.3 論文架構-------------------------------------------------------------------- 6 第二章 理論背景-------------------------------------------------------------- 7 2.1 低同調干涉術與系統解析度-------------------------------------------- 7 2.1.1 低同調光源------------------------------------------------------ 7 2.1.2 低同調干涉術(LCI)--------------------------------------------- 9 2.1.3 縱向解析度----------------------------------------------------- 13 2.1.4 橫向解析度----------------------------------------------------- 15 2.2 頻域光學同調斷層攝影術(SD-OCT)------------------------------- 18 2.3 掃瞄式光源之光學同調斷層攝影術(SS-OCT)--------------------- 20 第三章 實驗架構、原理與方法-------------------------------------------- 22 3.1 實驗儀器與系統架構------------------------------------------------ 22 3.1.1 光源-------------------------------------------------------------- 22 3.1.2 SS-OCT系統架構---------------------------------------------- 23 3.2 樣品處理與量測--------------------------------------------------------- 25 3.2.1 果蠅身體結構簡介-------------------------------------------- 25 3.2.2 果蠅培養-------------------------------------------------------- 27 3.2.3 果蠅處理與活體量測 ---------------------------------------- 27 3.3 果蠅突變種類說明------------------------------------------------------ 29 3.4 影像處理 ---------------------------------------------------------------- 30 3.4.1 二維(2D)連續影像(M-mode) ------------------------------- 30 3.4.2 心跳率(Heart Rate,HR)之處理 -------------------------- 32 3.4.3 心臟舒張(EDD)與收縮(ESD)之處理 -------------------- 36 3.4.4 心肌縮短分率(Fractional Shortening,FS)之計算方法 ------------------------------------------------------------------- 38 第四章 實驗結果與討論---------------------------------------------------- 39 4.1 系統空間解析度量測--------------------------------------------------- 39 4.1.1 縱向解析度----------------------------------------------------- 39 4.1.2 橫向解析度----------------------------------------------------- 41 4.2 量測結果------------------------------------------------------------------ 44 4.2.1 二維(2D)影像--------------------------------------------------- 44 4.3 資料分析 ----------------------------------------------------------------- 46 4.3.1 量測正常標準種類果蠅(w1118)與兩種心臟突變(mutation)之果蠅(Mut8i、Mut9i) ----------------------------------------46 4.3.1.1 公(male)與母(female)Data之比較 ---------------- 46 4.3.1.2 正常標準種類(standard strain)果蠅(w1118)與心臟突變(mutation)果蠅(Mut8i、Mut9i)心跳率(Heart Rate,HR)之比較 ------------------------------------- 50 4.3.1.3 正常標準種類(standard strain)果蠅(w1118)與心臟突變(mutation)果蠅(Mut8i、Mut9i) 心肌短縮分率(fractional shortening,FS)之比較 ----------------- 53 4.3.2 量測正常標準種類果蠅(w1118)與另外五種心臟突變(mutation)之果蠅(M8i、M9i、Δ164F1、Δ168M1、UAS-Acer)----------------------------------------------------- 60 4.3.2.1 w1118與M8i、M9i、Δ164F1、Δ168M1、UAS-Acer心臟收縮維度(ESD)之比較-------------------------- 60 4.3.2.2 w1118與M8i、M9i、Δ164F1、Δ168M1、UAS-Acer心肌短縮分率(fractional shortening,FS)之比較----------------------------------------------------------- 64 4.3.2.3 w1118與M8i、M9i、Δ164F1、Δ168M1、UAS-Acer之量測結論---------------------------------------------- 68 第五章 結論與未來展望---------------------------------------------- 69 5.1 討論------------------------------------------------------------------------ 69 5.2 結論-------------------------------------------------------------------------73 5.3 未來展望------------------------------------------------------------------ 75 參考文獻------------------------------------------------------------------------- 76

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