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研究生: 詹哲泓
論文名稱: 光學同調斷層造影術對輻射誘發腫瘤細胞侵襲能力之定量分析
指導教授: 郭文娟
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2008
畢業學年度: 96
語文別: 英文
論文頁數: 41
中文關鍵詞: 子宮頸癌肝癌侵襲能力放射線
英文關鍵詞: Cervical cancer, Hepatocellular carcinoma, Invasion, Radiation
論文種類: 學術論文
相關次數: 點閱:113下載:3
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  • 實驗中有效的利用三維快速高解析度掃描式光學同調斷層儀(SS-OCT)系統,來分析肝癌細胞(HCC)經過放射線照射後之侵犯能力。 量測過程中,分別在0、12、24、48 (hrs) 等不連續的時間點,利用SS-OCT系統來取得多張2維的光學同調斷層(OCT)影像。 實驗結果我們發現大部分細胞集中在膠的表層(depth<0.18 mm),僅有少部份具有較高侵犯能力的細胞能遷移至較深層(depth>0.18 mm)的部位。 故本研究利用SS-OCT之非侵入式的優點,分別針對受放射線刺激前後的細胞,對其侵犯程度的特性及遷移距離進行量化分析。

    Index...I Abstract (Chinese)...II Abstract...III List of figures...IV Chapter 1 Introduction...1 Chapter 2 Theory...3 2.1 The property of cancer cell...3 2.2 Cervical carcinoma (CC)...4 2.3 Hepatocellular carcinoma (HCC)...4 2.4 Radiation therapy...5 2.5 Radiation-promoted invasion of cancer cells...5 Chapter 3 Material and Methods...7 3.1 Cell preparation...7 3.2 SS-OCT imaging technique...8 3.3 In vitro measurement and data analysis...9 Chapter 4 Experimental Results...10 4.1 OCT Signals from cells mixed in Matrigel...10 4.2 OCT Signals from cells seeding on the surface of Matrigel...12 4.2.1 Cell invasion extent measurement...13 4.2.2 Cell migration distance measurement...15 4.3 Invasion capability of cervical cancer (CC) cells...16 4.4 Invasion capability of Hepatocellular carcinoma(HCC) cells...22 4.5 Conventional histology staining measurement...34 Chapter 5 Discussion...36 Chapter 6 Conclusion and Future Development...38 Refeences...39

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