透過製備高排列性銀奈米晶體樣品基板的設計來解決在表面輔助游離/脫附質譜最佳加熱點的問題，同時也是擴大應用性的關鍵所在。在本篇論文中，我們使用形狀均勻的銀奈米晶體作為大規模且高排列Langmuir-Blodgett（LB）膜的材料，且將其應用在SALDI-MS中做為無需基質的高效能樣品基板，主要使用的材料有三種不同形狀的銀奈米晶體如正立方體，十四面體，以及八面體做成的LB膜來做為SALDI-MS中檢測葡萄糖的樣品基板。
由結果與一般使用的CHCA和DHB基質相比較之下，使用銀奈米晶體做成的LB膜樣品版其訊號強度，雜訊訊號比，背景雜訊，以及再現性都得到顯著的提高。在這之中特別的是，當使用銀奈米十四面體做成的LB膜可以獲得5.7%的相對訊號。歸功於雷射照射能量的均勻分散使得我們在SALDI-MS測量中獲得非常好的改進，以及在高排列性銀十四面體LB膜下產生了大面積的最佳加熱區域，這種可以立即使用的樣品基板在SALDI-MS中已經顯示出了很大的商業應用機會。

The design of a homogeneous sample plate to solve the sweet heating spot issues is the key step to expand the applicability in surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). Here, large-scale and highly oriented Langmuir-Blodgett (LB) films of uniform-shaped silver nanocrystals have been fabricated as a highly efficient and matrix-free sample plate in SALDI MS. Three individual silver nanocrystals such as cube, cuboctahedron and octahedron assembled LB films have been applied as the sample plates for glucose detections in SALDI-MS without additional matrix. The results show that the signal intensity, background noise, signal-to-noise ratio and reproducibility have been significantly improved by using the LB films as the sample plate in comparison with commercial matrixes of CHCA and DHB. In particular, the relative signal of 5.7% has been obtained when the LB film of silver cuboctahedron has been applied. The great improvement in the SALDI-MS measurement could be attributed to homogenous dissipation of the laser irradiation energy to create a large area of the sweet heating spot on well-oriented silver cuboctahedron LB film. This ready-to-use sample plate has revealed great commercial applications in SALDI-MS.

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