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研究生: 張富翔
論文名稱: 磁流體熱治療之系統整合及應用於肝癌治療
System Integration and Application of Liver Cancer Treatment for Magnetic Fluid Hyperthermia
指導教授: 謝振傑
Chieh, Jen-Jie
廖書賢
Liao, Shu-Hsien
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2014
畢業學年度: 102
語文別: 中文
論文頁數: 47
中文關鍵詞: 熱治療肝癌磁流體
論文種類: 學術論文
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  • 磁流體熱治療是利用磁流體在外加交流磁場中發熱的特性,使腫瘤組織的溫度超過其耐熱溫度而被破壞,達到熱治療的目的。本實驗將磁性試劑注射到癌鼠腫瘤中,施加交流磁場進行熱治療,並同時使用光纖溫度計以及紅外線熱像儀量測組織升溫情形,腫瘤區域溫度超過42℃,超過細胞的耐熱溫度,經由追蹤量測體積發現腫瘤體積有逐漸變小的趨勢,也藉由TUNEL染色驗證腫瘤區域內經熱治療後細胞大量死亡,達到熱治療的目的。

    第一章 緒論.............................................1 第二章 實驗原理..........................................2 2-1 磁流體升溫機制........................................2 第三章 實驗架構與方法.....................................8 3-1 磁流體熱治療實驗硬體架構................................8 3-2 磁流體熱治療實驗軟體整合...............................10 3-3 磁流體熱治療試劑製程..................................11 3-3-1水基磁性奈米流體製作方法..............................11 3-3-2水基磁流體物理特性量測................................13 3-4細胞實驗.............................................19 3-4-1細胞存活率分析( MTT Assay)..........................19 3-4-2磁場對細胞的影響....................................19 3-4-3 試劑對細胞的影響...................................21 3-5 動物實驗............................................23 3-5-1 無腫瘤老鼠注射試劑實驗 ..............................23 3-5-2 有腫瘤老鼠注射試劑實驗 ..............................25 3-5-3 無熱治療的癌鼠腫瘤體積量測............................26 3-6 TUNEL 染色檢測 (TUNEL Assay)........................26 第四章 實驗結果.........................................28 4-1掃描式超導生醫感測儀 (SSB) 量測結果......................28 4-2 光纖溫度計量測結果....................................31 4-3 紅外線溫度攝影機結果..................................33 4-4 TUNEL 染色結果......................................35 4-5 老鼠腫瘤體積變化......................................39 第五章 結論.............................................40 參考文獻.................................................41

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