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研究生: 張嘉珍
Chia Chen Chang
論文名稱: 增補複方炙甘草湯對單次衰竭運動後血液脂質與氧化指標的影響
The effects of Fufang Zhigancao Tang supplementation on exercise-induced lipid oxidation and antioxidant markers
指導教授: 謝伸鬙
Hsieh, Shen-Yu
林嘉志
Lin, Chia-Chih
學位類別: 碩士
Master
系所名稱: 體育學系
Department of Physical Education
論文出版年: 2005
畢業學年度: 94
語文別: 中文
論文頁數: 59
中文關鍵詞: 中藥複方炙甘草湯運動氧化傷害
英文關鍵詞: Chinese herb, Fufang Zhigancao Tang, exercise, oxidative damage
論文種類: 學術論文
相關次數: 點閱:264下載:26
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  • 目的:探討長期增補複方炙甘草湯後進行單次高強度運動,對氧化壓力的變化與心血管疾病的影響。方法:以18位健康大學男性,依VO2max高低為次序平衡之依據分為兩組:炙甘草組與安慰劑組,實驗處理為期四週。以高強度運動 ( 85%VO2max ) 評估增補後對氧化壓力的變化。在增補前、後與運動前、後分別採集血液樣本分析catalase ( CAT ) 活性、superoxide dismutase ( SOD ) 活性、malondialdehyde ( MDA ) 濃度、low-density lipoprotein cholesterol ( LDL-C ) 濃度、high-density lipoprotein cholesterol ( HDL-C ) 濃度與oxidize low-density lipoprotein ( oxLDL ) 濃度等生化分析。所得數據以獨立樣本 t 考驗、混合設計雙因子(組別 × 時序)變異數分析 ( ANOVA ) 與混合設計雙因子(組別 × 時序)共變數分析 ( ANCOVA ) 檢定。結果:四週增補後發現紅血球溶胞液CAT與SOD活性、血漿LDL-C與HDL-C在組間與時序的比較皆未達顯著差異。兩組受試者在運動後的血漿乳酸濃度( 7.65 ± 0.90 mM ) 顯著高於運動前 ( 1.51 ± 0.48 mM, p<.05 )。炙甘草組的血漿MDA ( 2.58 ± 0.39 mM ) 在增補四週後顯著低於安慰劑組 ( 3.61 ± 0.26 mM, p<.05 )。同樣地,炙甘草組在運動後之MDA ( 1.63 ± 0.11 mM ) 也顯著低於安慰劑組 ( 2.81 ± 0.33 mM, p<.05 )。炙甘草組在增補後之血漿oxLDL ( 5.62 ± 0.74 U ) 顯著低於增補前 ( 6.56 ± 0.69 U, p<.05 )。結論:持續四週增補複方炙甘草湯後,可防止從事單次高強度運動後自由基對脂質造成的損傷,有助於保護脂質,降低血漿MDA與oxLDL的濃度,但無法改變血漿HDL-C與LDL-C濃度的變化,推測可能與增補的劑量、時間長短或吸收代謝有關。本實驗推測增補複方炙甘草湯可能有助於預防心血管疾病。

    Purpose: To investigate the effects of Fufang Zhigancao Tang supplementation on oxidative damage and cardiovascular risk factors after one bout of high intensive exercise. Methods: Eighteen healthy college males were assigned into two groups ( Zhigancao group and placebo group ) according to VO2max. Experimental treatment period lasted for four weeks. At the end of treatment, a bout of high intensive exercise was performed to observe changes of oxidative stress. Blood samples were collected before, after supplementation and immediately after exercise. Venous blood samples were analyzed for catalase (CAT) activity, superoxide dismutase (SOD) acivity, malondialdehyde (MDA), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and oxidized low-density lipoprotein (oxLDL). Independent t test, mixed design of two-way ( group × time ) ANOVA and ANCOVA were used for data analysis. Results: After four weeks of supplementation, CAT activity, SOD activity, LDL-C and HDL-C in erythrocyte lysate showed no significant differences between groups and times. Plasma lactate was significantly higher after exercise ( 7.65 ± 0.90 mM ) than that of before ( 1.51 ± 0.48 mM, p<.05 ). Compared with placebo group ( 3.61 ± 0.2 mM ), plasma MDA in Zhigancao group ( 2.58 ± 0.39 mM ) was significantly lower after supplementation ( p<.05 ). There was a smiliar result after exercise. Plasma MDA in Zhigancao group ( 1.63 ± 0.11 mM ) after exercise was significantly lower than that in placebo group ( 2.81 ± 0.33, p<.05 ). Plasma oxLDL in Zhigancao group ( 5.62 ± 0.74 U ) was significantly lower than that in placebo group ( 6.56 ± 0.69 U, p<.05 ). Conclusion: After four-week supplementation of Fufang Zhigancao Tang, it is effective in preventing lipid damage such as decrease of plasma MDA and oxLDL after a bout of high intensity exercise. However, plasma HDL-C and LDL-C were not changed. It may be related with dosage, treatment duration and metabolism. This study may provide evidence to support potentiality of Fufang Zhigancao Tang on prevention of cardiovascular disease.

    碩士論文通過簽名書……………………………………………… i 碩士論文授權書……………………………………………………. ii 中文摘要..…………………………………………………………... iii 英文摘要..…………………………………………………………... iv 謝誌.…………………………………………………………………. v 目次………………………………………………………………….. vi 表次………………………………………………………………….. ix 圖次………………………………………………………………….. x 第壹章 緒論...…………………………………………………….. 1 一、問題背景…………………………………………………….. 1 二、研究目的…………………………………………………….. 3 三、研究假設…………………………………………………….. 3 四、名詞操作性定義…………………………………………….. 3 第貳章 文獻探討…...…………………………………………….. 5 一、自由基與氧化壓力………………………………………….. 5 二、運動訓練與氧化壓力……………………………………….. 6 三、炙甘草、人參、麥冬與心血管功能………………………….. 8 四、複方炙甘草湯與氧化壓力…………………………………… 11 第參章 研究方法與步驟………………………….………….... 14 一、材料與方法………………………………………………….. 14 二、實驗流程…………………………………………………….. 16 三、實驗設計…………………………………………………….. 17 四、血液樣本分析……………………………………………….. 18 五、統計分析…………………………………………………….. 18 第肆章 結果……………………………………………………… 19 一、受試者基本資料……………………………………….. 19 二、實驗期間能量消耗之比較………………………………… 19 三、單次高強度運動之運動表現……………………………….. 20 四、抗氧化酶活性……………………………………………… 20 (一)過氧化氫酶 ( CAT )………………………………………. 20 (二)過氧化物歧化酶 ( SOD )………………………………… 21 五、脂質過氧化物……………………………………………… 22 六、血液脂質分析……………………………………………… 24 (一)低密度脂蛋白膽固醇…………………………………… 24 (二)高密度脂蛋白膽固醇…………………………………… 25 (三)氧化態低密度脂蛋白…………………………………… 26 七、乳酸……………………………………………………..…… 27 第伍章 討論與結論……………………………………………… 29 一、實驗控制……………………………………………..…… 29 二、複方炙甘草湯增補與運動對抗氧化酶活性與血漿MDA 的影響………………………………………………..……… 30 三、複方炙甘草湯增補與運動對心血管疾病指標的影響…… 32 四、增補複方炙甘草湯與運動對oxLDL的影響………….…… 34 五、結論與建議……………………………………………….… 35 主要參考文獻………………………………………………………. 36 一、中文部份…………….……………………………………….. 36 二、英文部份……………………………………………………... 39 附錄一 身體健康調查………………………………………...….. 45 附錄二 每日飲食調查………………………………………….... 46 附錄三 受試者同意書………………………………………..….. 47 附錄四 每日身體活動記錄表………………………………..….. 49 附錄五 血液生化分析….……………………………………..….. 52 附錄六 實驗數據……………………………………………...….. 58 個人小傳……………………………………………………………. 59

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