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研究生: 張嘉珍
Chia Chen Chang
論文名稱: 增補複方炙甘草湯對單次衰竭運動後血液脂質與氧化指標的影響
The effects of Fufang Zhigancao Tang supplementation on exercise-induced lipid oxidation and antioxidant markers
指導教授: 謝伸鬙
Hsieh, Shen-Yu
林嘉志
Lin, Chia-Chih
學位類別: 碩士
Master
系所名稱: 體育學系
Department of Physical Education
論文出版年: 2005
畢業學年度: 94
語文別: 中文
論文頁數: 59
中文關鍵詞: 中藥複方炙甘草湯運動氧化傷害
英文關鍵詞: Chinese herb, Fufang Zhigancao Tang, exercise, oxidative damage
論文種類: 學術論文
相關次數: 點閱:276下載:26
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  • 目的:探討長期增補複方炙甘草湯後進行單次高強度運動,對氧化壓力的變化與心血管疾病的影響。方法:以18位健康大學男性,依VO2max高低為次序平衡之依據分為兩組:炙甘草組與安慰劑組,實驗處理為期四週。以高強度運動 ( 85%VO2max ) 評估增補後對氧化壓力的變化。在增補前、後與運動前、後分別採集血液樣本分析catalase ( CAT ) 活性、superoxide dismutase ( SOD ) 活性、malondialdehyde ( MDA ) 濃度、low-density lipoprotein cholesterol ( LDL-C ) 濃度、high-density lipoprotein cholesterol ( HDL-C ) 濃度與oxidize low-density lipoprotein ( oxLDL ) 濃度等生化分析。所得數據以獨立樣本 t 考驗、混合設計雙因子(組別 × 時序)變異數分析 ( ANOVA ) 與混合設計雙因子(組別 × 時序)共變數分析 ( ANCOVA ) 檢定。結果:四週增補後發現紅血球溶胞液CAT與SOD活性、血漿LDL-C與HDL-C在組間與時序的比較皆未達顯著差異。兩組受試者在運動後的血漿乳酸濃度( 7.65 ± 0.90 mM ) 顯著高於運動前 ( 1.51 ± 0.48 mM, p<.05 )。炙甘草組的血漿MDA ( 2.58 ± 0.39 mM ) 在增補四週後顯著低於安慰劑組 ( 3.61 ± 0.26 mM, p<.05 )。同樣地,炙甘草組在運動後之MDA ( 1.63 ± 0.11 mM ) 也顯著低於安慰劑組 ( 2.81 ± 0.33 mM, p<.05 )。炙甘草組在增補後之血漿oxLDL ( 5.62 ± 0.74 U ) 顯著低於增補前 ( 6.56 ± 0.69 U, p<.05 )。結論:持續四週增補複方炙甘草湯後,可防止從事單次高強度運動後自由基對脂質造成的損傷,有助於保護脂質,降低血漿MDA與oxLDL的濃度,但無法改變血漿HDL-C與LDL-C濃度的變化,推測可能與增補的劑量、時間長短或吸收代謝有關。本實驗推測增補複方炙甘草湯可能有助於預防心血管疾病。

    Purpose: To investigate the effects of Fufang Zhigancao Tang supplementation on oxidative damage and cardiovascular risk factors after one bout of high intensive exercise. Methods: Eighteen healthy college males were assigned into two groups ( Zhigancao group and placebo group ) according to VO2max. Experimental treatment period lasted for four weeks. At the end of treatment, a bout of high intensive exercise was performed to observe changes of oxidative stress. Blood samples were collected before, after supplementation and immediately after exercise. Venous blood samples were analyzed for catalase (CAT) activity, superoxide dismutase (SOD) acivity, malondialdehyde (MDA), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and oxidized low-density lipoprotein (oxLDL). Independent t test, mixed design of two-way ( group × time ) ANOVA and ANCOVA were used for data analysis. Results: After four weeks of supplementation, CAT activity, SOD activity, LDL-C and HDL-C in erythrocyte lysate showed no significant differences between groups and times. Plasma lactate was significantly higher after exercise ( 7.65 ± 0.90 mM ) than that of before ( 1.51 ± 0.48 mM, p<.05 ). Compared with placebo group ( 3.61 ± 0.2 mM ), plasma MDA in Zhigancao group ( 2.58 ± 0.39 mM ) was significantly lower after supplementation ( p<.05 ). There was a smiliar result after exercise. Plasma MDA in Zhigancao group ( 1.63 ± 0.11 mM ) after exercise was significantly lower than that in placebo group ( 2.81 ± 0.33, p<.05 ). Plasma oxLDL in Zhigancao group ( 5.62 ± 0.74 U ) was significantly lower than that in placebo group ( 6.56 ± 0.69 U, p<.05 ). Conclusion: After four-week supplementation of Fufang Zhigancao Tang, it is effective in preventing lipid damage such as decrease of plasma MDA and oxLDL after a bout of high intensity exercise. However, plasma HDL-C and LDL-C were not changed. It may be related with dosage, treatment duration and metabolism. This study may provide evidence to support potentiality of Fufang Zhigancao Tang on prevention of cardiovascular disease.

    碩士論文通過簽名書……………………………………………… i 碩士論文授權書……………………………………………………. ii 中文摘要..…………………………………………………………... iii 英文摘要..…………………………………………………………... iv 謝誌.…………………………………………………………………. v 目次………………………………………………………………….. vi 表次………………………………………………………………….. ix 圖次………………………………………………………………….. x 第壹章 緒論...…………………………………………………….. 1 一、問題背景…………………………………………………….. 1 二、研究目的…………………………………………………….. 3 三、研究假設…………………………………………………….. 3 四、名詞操作性定義…………………………………………….. 3 第貳章 文獻探討…...…………………………………………….. 5 一、自由基與氧化壓力………………………………………….. 5 二、運動訓練與氧化壓力……………………………………….. 6 三、炙甘草、人參、麥冬與心血管功能………………………….. 8 四、複方炙甘草湯與氧化壓力…………………………………… 11 第參章 研究方法與步驟………………………….………….... 14 一、材料與方法………………………………………………….. 14 二、實驗流程…………………………………………………….. 16 三、實驗設計…………………………………………………….. 17 四、血液樣本分析……………………………………………….. 18 五、統計分析…………………………………………………….. 18 第肆章 結果……………………………………………………… 19 一、受試者基本資料……………………………………….. 19 二、實驗期間能量消耗之比較………………………………… 19 三、單次高強度運動之運動表現……………………………….. 20 四、抗氧化酶活性……………………………………………… 20 (一)過氧化氫酶 ( CAT )………………………………………. 20 (二)過氧化物歧化酶 ( SOD )………………………………… 21 五、脂質過氧化物……………………………………………… 22 六、血液脂質分析……………………………………………… 24 (一)低密度脂蛋白膽固醇…………………………………… 24 (二)高密度脂蛋白膽固醇…………………………………… 25 (三)氧化態低密度脂蛋白…………………………………… 26 七、乳酸……………………………………………………..…… 27 第伍章 討論與結論……………………………………………… 29 一、實驗控制……………………………………………..…… 29 二、複方炙甘草湯增補與運動對抗氧化酶活性與血漿MDA 的影響………………………………………………..……… 30 三、複方炙甘草湯增補與運動對心血管疾病指標的影響…… 32 四、增補複方炙甘草湯與運動對oxLDL的影響………….…… 34 五、結論與建議……………………………………………….… 35 主要參考文獻………………………………………………………. 36 一、中文部份…………….……………………………………….. 36 二、英文部份……………………………………………………... 39 附錄一 身體健康調查………………………………………...….. 45 附錄二 每日飲食調查………………………………………….... 46 附錄三 受試者同意書………………………………………..….. 47 附錄四 每日身體活動記錄表………………………………..….. 49 附錄五 血液生化分析….……………………………………..….. 52 附錄六 實驗數據……………………………………………...….. 58 個人小傳……………………………………………………………. 59

    一、中文部份
    中國藥典委員會 (1991)。中國藥典。台北市:旺文。
    王尊鳳、胡卡明、蘇金生 (1997)。怡神(紅景天)膠囊抗疲勞和提高運動能力的作用。成都中醫藥大學學報,20(2),35-38。
    行政院衛生署 (2004)。民國93年縣市死因統計。2005年1月10日,取自行政院衛生署,衛生統計資訊網網址http://www.doh.gov.tw/statistic/index.htm
    李占永、岳雪蓮 (1996)。中藥複方化學成分研究近況。中國實驗方劑學雜誌,2(1),41-44。
    余伯陽、殷霞、榮祖元 (1994)。短葶山麥冬皂甙C的藥理活性研究。中國藥科大學學報,25(5),286-288。
    呂昌明、林旭龍、黃奕清、李明憲、王淑芳 (2000)。身體活動自我報告量表之效度及信度的研究—以Polar Vantage NV心博率監測器為效標。衛生教育學報,14,33-48。
    李政木、王蕭 (1998)。炙甘草湯治療早搏83例的臨床觀察。福建中醫藥,29(5),1-2。
    吳家慶(2005)。不同強度動態恢復對損傷肌肉之能及跑步經濟性的影響。未出版博士論文,國立台灣師範大學,台北市。
    宋曉亮、高廣猷、葉麗虹(1996)。山麥冬總皂甙對實驗性心肌缺血的影響。中國藥理學通報,12(4),329-332。
    林勁宏 (2000)。運動與脂質氧化傷害。中華體育,14(3),78-86。
    林奕良、李君珮、林嘉志 (2005,12月)。炙甘草湯飲用對大鼠心血管抗氧化能力的影響:年輕鼠與中老年鼠的比較﹝摘要﹞。第四屆華人運動生理及體適能學者學會海報發表,台北市。
    周建偉 (1996)。益氣滋陰法治療冠心病36例。湖南中醫藥雜誌,12(5),5。
    胡因銘、陳奇 (1995)。炙甘草湯對大鼠實驗性心肌缺血再灌注損傷的影響。中國實驗方劑學雜誌,1(1),18-21。
    俞昌琪、彭小冰、鄭邦英、尹琦(1999)。炙甘草湯抗衰老等作用的實驗觀察。貴陽中醫學院學報,21(1),59-60。
    姚承義 (2002)。抗氧化劑增補對激烈運動及魚油引發之氧化壓力的影響 (博士論文,國立臺灣師範大學,2000)。全國博碩士論文資訊網,090NTNU0567005。
    趙克然、楊毅軍、曹道俊 (2000)。氧自由基與臨床。台北市:合記。
    徐台閣 (1997)。半程馬拉松對自由基及自然殺手細胞之影響。我國大專運動科學86年度研究獎助專刊,135-148。
    桂苡、高廣猷(1984)。山麥冬對心血管系統藥理作用的研究。中草藥,15(3),21-23。
    宮曙光、凌樹森、金和生 (1995)。炙甘草湯、二參通脈湯的藥理作用。藥學實踐雜誌,13(1),42-43。
    夏炎興、袁燦興 (1993)。炙甘草抗心律失常的實驗研究。中國中醫藥科技,1(4),25-29。
    連曉媛、陳奇、畢明 (1993)。炙甘草湯抗心律失常實驗研究。中藥藥理與臨床,9(6),1-3。
    連曉媛、陳奇、畢明 (1994)。炙甘草湯對心肌缺血再灌注損傷的保護作用。中藥藥理與臨床,10(5),6-8。
    陳立波、佟力、趙洪序 (1994)。人參總皂甙對心肌缺血再灌注損傷的保護作用實驗研究。中華胸心血管外科雜誌,10(4),353-354。
    陳奇 (1993)。動物實驗研究方法及其在中醫研究中的應用。載於陳奇主編,中藥藥理研究方法學(頁885)。北京:人民衛生出版社。
    許玲、張申 (1996)。炙甘草、黃耆、黨參對心氣虛患者心臟功能及血管狀況的影響。實用中醫藥雜誌,12(2),28-29。
    黃國欽、邱亦涵、徐台閣 (2003)。運動誘發的氧化壓力對DNA傷害之探討。中華體育,17(4),26-33。
    曹霞、高宇飛、李紅、楊紅、楊世傑、杜雪榮 (2001)。人參、西洋參及刺五加皂甙對離體工作心臟作用的對比研究。白求恩醫科大學學報,27(3),246-248。
    楊淑媚 (2004)。人參皂甙Rh2抑制肺腺癌細胞生長及造成凋亡之分子作用機轉研究(碩士論文,中國醫藥大學,2003)。全國博碩士論文資訊網,092CMCH0050013。
    廖威彰 (2000)。衰竭運動對老鼠肺臟、心臟、肝臟及腎臟脂質過氧化物的影響。大專體育學刊,2(1),109-118。
    劉接寶 (2006)。傷寒論助讀。台北市:立得。
    盧宏民 (1983)。中藥大辭典。台北市:五洲。
    嚴永清 (1985)。關於加強中藥複方化學成分的研究。中藥通報,10(2),35。

    二、英文部份
    Aguilo, A., Tauler, P., Pilar Guix, M., Villa, G., Cordova, A., Tur, J. A., et al. (2003). Effect of exercise intensity and training on antioxidants and cholesterol profile in cyclists. The Journal of Nutritional Biochemistry, 14(6), 319-325.
    American college of sports medicine. (2005). ACSM's guidelines for exercise testing and prescription (7th ed.). Philadelphia: Lippincott Williams and Wilkins.
    Ames, B. N. (1998). Micronutrients prevent cancer and delay aging. Toxicology Letters, 102-103, 5-18.
    Brites, F., Zago, V., Verona, J., Muzzio, M. L., Wikinski, R., & Schreier, L. (2006). HDL capacity to inhibit LDL oxidation in well-trained triathletes. Life Science, 78(26), 3074-3081.
    Castelli, W. P. (1988). Cholesterol and lipids in the risk of coronary artery disease--the Framingham Heart Study. Canadian Journal of Cardiology, 4(Suppl A), 5A-10A.
    Child, R. B., Wilkinson, D. M., Fallowfield, J. L., & Donnelly, A. E. (1998). Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half-marathon run. Medicine and Science in Sports and Exercise, 30(11), 1603-1607.
    Cox, D. A., & Cohen, M. L. (1996). Effects of oxidized low-density lipoprotein on vascular contraction and relaxation: clinical and pharmacological implications in atherosclerosis. Pharmacological Reviews, 48(1), 3-19.
    Dandona, P., Thusu, K., Cook, S., Snyder, B., Makowski, J., Armstrong, D., et al. (1996). Oxidative damage to DNA in diabetes mellitus. Lancet, 347(8999), 444-445.
    Davies, K. J., Quintanilha, A. T., Brooks, G. A., & Packer, L. (1982). Free radicals and tissue damage produced by exercise. Biochemical of Biophysical Research Communications, 107(4), 1198-1205.
    Del Rio, D., Stewart, A. J., & Pellegrini, N. (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, Metabolism, and Cardiovascular Diseases, 15(4), 316-328.
    Dhalla, N. S., Temsah, R. M., & Netticadan, T. (2000). Role of oxidative stress in cardiovascular diseases. Journal of Hypertension, 18(6), 655-673.
    Di Meo, S., & Venditti, P. (2001). Mitochondria in exercise-induced oxidative stress. Biological Signals Receptors, 10(1-2), 125-140.
    Durstine, J. L., Grandjean, P. W., Cox, C. A., & Thompson, P. D. (2002). Lipids, lipoproteins, and exercise. Journal of Cardiopulmonary Rehabilitation., 22(6), 385-398.
    Duthie, G. G., Robertson, J. D., Maughan, R. J., & Morrice, P. C. (1990). Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Archives of Biochemistry and Biophysics, 282(1), 78-83.
    Ferrando, A., Vila, L., Voces, J. A., Cabral, A. C., Alvarez, A. I., & Prieto, J. G. (1999). Effects of ginseng extract on various haematological parameters during aerobic exercise in the rat. Planta Medica, 65(3), 288-290.
    Finkel, T., & Holbrook, N. J. (2000). Oxidants, oxidative stress and the biology of ageing. Nature, 408(6809), 239-247.
    Groussard, C., Rannou-Bekono, F., Machefer, G., Chevanne, M., Vincent, S., Sergent, O., et al. (2003). Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise. European Journal of Applied Physiology, 89(1), 14-20.
    Gul, M., Demircan, B., Taysi, S., Oztasan, N., Gumustekin, K., Siktar, E., et al. (2006). Effects of endurance training and acute exhaustive exercise on antioxidant defense mechanisms in rat heart. Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology, 143(2), 239-245.
    Hammond, B., & Hess, M. L. (1985). The oxygen free radical system: potential mediator of myocardial injury. Journal of American College of Cardiology, 6(1), 215-220.
    Harry, D., Anand, R., Holt, S., Davies, S., Marley, R., Fernando, B., et al. (1999). Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic rat. Hepatology, 30(5), 1198-1205.
    Hillis, L. D., & Braunwald, E. (1977). Myocardial ischemia (second of three parts). The New England Journal of Medicine, 296(18), 1034-1041.
    Kanter, M. M., Lesmes, G. R., Kaminsky, L. A., La Ham-Saeger, J., & Nequin, N. D. (1988). Serum creatine kinase and lactate dehydrogenase changes following an eighty kilometer race. Relationship to lipid peroxidation. European Journal of Applied Physiology and Occupational Physiology, 57(1), 60-63.
    Kevin, L. G., Novalija, E., & Stowe, D. F. (2005). Reactive oxygen species as mediators of cardiac injury and protection: the relevance to anesthesia practice. Anesthesia and Analgesia, 101(5), 1275-1287.
    Koska, J., Blazicek, P., Marko, M., Grna, J. D., Kvetnansky, R., & Vigas, M. (2000). Insulin, catecholamines, glucose and antioxidant enzymes in oxidative damage during different loads in healthy humans. Physiological Research, 49 Suppl 1, S95-100.
    Leeuwenburgh, C., & Heinecke, J. W. (2001). Oxidative stress and antioxidants in exercise. Current Medicinal Chemistry, 8(7), 829-838.
    Lin, C. C., & Huang, P. C. (2000). Antioxidant and hepatoprotective effects of acathopanax senticosus. Phytotherapy Research, 14(7), 489-494.
    McMillan, K., Helgerud, J., Grant, S. J., Newell, J., Wilson, J., Macdonald, R., et al. (2005). Lactate threshold responses to a season of professional British youth soccer. British Journal of Sports Medicine, 39(7), 432-436.
    Miyazaki, H., Oh-ishi, S., Ookawara, T., Kizaki, T., Toshinai, K., Ha, S., et al. (2001). Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. European Journal of Applied Physiology, 84(1-2), 1-6.
    Oostenbrug, G. S., Mensink, R. P., Hardeman, M. R., De Vries, T., Brouns, F., & Hornstra, G. (1997). Exercise performance, red blood cell deformability, and lipid peroxidation: effects of fish oil and vitamin E. Journal of Applied Physiology, 83(3), 746-752.
    Ou, B., Huang, D., Hampsch-Woodill, M., & Flanagan, J. A. (2003). When east meets west: the relationship between yin-yang and antioxidation-oxidation. Official Publication of the Federation of American Societies for Experimental Biology, 17(2), 127-129.
    Packer, L. (1997). Oxidants, antioxidant nutrients and the athlete. Journal of Sports Science, 15(3), 353-363.
    Powers, S. K., Ji, L. L., & Leeuwenburgh, C. (1999). Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review. Medicine and Science in Sports and Exercise, 31(7), 987-997.
    Prior, R. L., Cao, G., Prior, R. L., & Cao, G. (2000). Analysis of botanicals and dietary supplements for antioxidant capacity: A review. Journal of AOAC International, 83(4), 950-956.
    Santos-Silva, A., Rebelo, M. I., Castro, E. M., Belo, L., Guerra, A., Rego, C., et al. (2001). Leukocyte activation, erythrocyte damage, lipid profile and oxidative stress imposed by high competition physical exercise in adolescents. Clinica Chimica Acta, 306(1-2), 119-126.
    Sastre, J., Asensi, M., Gasco, E., Pallardo, F. V., Ferrero, J. A., Furukawa, T., et al. (1992). Exhaustive physical exercise causes oxidation of glutathione status in blood: prevention by antioxidant administration. American Journal of Physiology, 263(5 Pt 2), R992-995.
    Sen, C. K. (1995). Oxidants and antioxidants in exercise. Journal of Applied Physiology, 79(3), 675-686.
    Sen, C. K., Atalay, M., & Hanninen, O. (1994). Exercise-induced oxidative stress: Glutathione supplementation and deficiency. Journal of Applied Physiology, 77(5), 2177-2187.
    Steinberg, D., Parthasarathy, S., Carew, T. E., Khoo, J. C., & Witztum, J. L. (1989). Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. The New England Journal of Medicine, 320(14), 915-924.
    Tamer, L., Sucu, N., Polat, G., Ercan, B., Aytacoglu, B., Yucebilgic, G., et al. (2002). Decreased serum total antioxidant status and erythrocyte-reduced glutathione levels are associated with increased serum malondialdehyde in atherosclerotic patients. Archives of Medical Research, 33(3), 257-260.
    Tavazzi, B., Di Pierro, D., Bartolini, M., Marino, M., Distefano, S., Galvano, M., et al. (1998). Lipid peroxidation, tissue necrosis, and metabolic and mechanical recovery of isolated reperfused rat heart as a function of increasing ischemia. Free Radical Research, 28(1), 25-37.
    Terblanche, S. E. (2000). The effects of exhaustive exercise on the activity levels of catalase in various tissues of male and female rats. Cell Biology International, 23(11), 749-753.
    Thompson, P. D., Crouse, S. F., Goodpaster, B., Kelley, D., Moyna, N., & Pescatello, L. (2001). The acute versus the chronic response to exercise. Medicine and Science in Sports and Exercise, 33(6 Suppl), S438-445; discussion S452-433.
    Urso, M. L., & Clarkson, P. M. (2003). Oxidative stress, exercise, and antioxidant supplementation. Toxicology, 189(1-2), 41-54.
    Van Soeren, M. H., & Graham, T. E. (1998). Effect of caffeine on metabolism, exercise endurance, and catecholamine responses after withdrawal. Journal of Applied Physiology, 85(4), 1493-1501.
    Vider, J., Lehtmaa, J., Kullisaar, T., Vihalemm, T., Zilmer, K., Kairane, C., et al. (2001). Acute immune response in respect to exercise-induced oxidative stress. Pathophysiology, 7(4), 263-270.
    Vina, J., Gomez-Cabrera, M. C., Lloret, A., Marquez, R., Minana, J. B., Pallardo, F. V., et al. (2000). Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB Life, 50(4-5), 271-277.
    Wang, J. S., Lee, T., & Chow, S. E. (2006). Role of Exercise Intensities in Oxidized-Low Density Lipoprotein-Mediated Redox Status of Monocyte in Men. Journal of Applied Physiology.
    Wetzstein, C. J., Shern-Brewer, R. A., Santanam, N., Green, N. R., White-Welkley, J. E., Parthasarathy, S. (1998). Does acute exercise affect the susceptibility of low density lipoprotein to oxidation? Free Radical Biology and Medicine, 24(4), 679-682.
    Yanai, H., & Morimoto, M. (2004). Effect of ascorbate on serum lipids and urate metabolism during exhaustive training. Clinical Science (Lond), 106(1), 107-109.

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