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研究生: 李宜諺
Li, Yi-Yan
論文名稱: 光與磁造影之超導生醫磁導儀應用於探討肝腫瘤的標靶造影
Scanning SQUID Biosusceptometery Applied to Exploring of Hepatocellular Carcinoma Targeted by Optics and Magnetic Dual-Mode Imaging.
指導教授: 謝振傑
Chieh, Jen-Jie
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2015
畢業學年度: 103
語文別: 中文
論文頁數: 29
中文關鍵詞: 磁性奈米粒子超導磁導儀批覆生物探針交流磁化率
論文種類: 學術論文
相關次數: 點閱:125下載:4
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  • 現今醫學影像方面如斷層掃描、核磁共振造影、X-Ray,都具有高解析度的斷層影像,但因為維護價格高與屏蔽系統需要夠良好,所以,往往只有大型醫療機構擁有。為了增加靈敏度與專一性,批覆生物探針的奈米粒子已成為主流,利用批覆胎兒蛋白(Alpha-fetoprotein,AFP)抗體、批覆去羧機凝血脢原(Des gamma carboxy prothrombin,DCP)、批覆酯肌醇蛋白聚醣3(Glypican-3,GPC3 )抗體的磁性奈米流體,注射於癌鼠做標靶實驗,並開發出光學與磁學雙模式的超導磁導儀檢測磁性奈米粒子的交流磁化率。本實驗除了用磁導儀對注射磁流體試劑的癌鼠進行檢測,利用核磁共振造影與肝臟五葉個別量測的一致性證實了磁導儀可行性。因此,未來病患可注射批覆抗體的磁性奈米粒子試劑後進行活體篩檢,如果有腫瘤症狀,需要進一步確認再進行高額的其他檢查,減少民眾負擔。

    目錄 摘要..........................I 目錄.......................... II 圖表目錄.......................... IV 第一章 緒論..........................1 第二章 研究理論.......................... 3 2.1 磁導儀磁訊號量測理論..........................3 第三章 磁性奈米粒子與標靶試劑檢測方法.....................9 3.1 磁性奈米流體備製..........................9 圖3.1批覆抗體磁性流體備置流程........................10 3.2激發線圈與感應線圈設計與製作..................10 圖3.2激發線圈側視與底視..........................11 3.3雙模式磁導儀系統設計與製作..........................11 第四章 實驗結果..........................13 4.1 雙模式探頭性能測試..........................13 4.1.1假體測試..........................13 4.1.2磁性奈米粒子流體不同濃度相同深度量測..........13 4.2動物研究測試..........................16 4.2.1 實驗規劃..........................16 4.2.2影像疊合與間歇性量測測試.................16 4.2.3背部腫瘤..........................17 4.2.4肝臟腫瘤..........................18 第五章 結論..........................24 參考文獻..........................25 致謝..........................28 圖表目錄 圖 2.1生物磁場強度與超導量子干涉儀的靈敏度........4 圖2.2磁量耦合示意圖..........................6 圖2.3 rf SQUID與共振電路形成互感的等效電路........7 圖2.4為SQUID工作電路..........................8 圖3.1批覆抗體磁性流體備置流程..........................10 圖3.2激發線圈側視與底視..........................11 圖3.3光學與磁學雙模式造影系統..........................12 圖4.1假體測試磁影像與強度比較..........................13 圖4.3 不同MF濃度與不同探頭深度,光學與磁學影像疊合.....15 圖4.4不同探頭深度不同與MF濃度之強度總合比較............15 圖4.5標準曲線圖..........................15 圖4.6間歇掃描檢查[26]、連續靜態檢查........17 圖4.7背部腫瘤老鼠注射試劑前後強度影像.......18 圖4.8掃描範圍與不同時間量測強度比較........20 圖4.9核磁共振造影與訊號比較................21 圖4.10手術引導肝腫瘤之磁導儀量測............22 圖4.11不同試劑不同時間點量測強度比較.........23

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