國立臺灣師範大學博碩士論文全文系統

簡易檢索 / 詳目顯示

回結果列表

研究生：	熊名煒 Ming-Wei Hsiung
論文名稱：	極化光學同調斷層攝影術(PS-OCT)對人體血管組織光學特性之定量分析 Quantitative analysis on optical properties of human vessel tissue by using polarization-sensitive optical coherence tomography (PS-OCT)
指導教授：	郭文娟 Kuo, Wen-Chuan
學位類別：	碩士 Master
系所名稱：	光電工程研究所 Graduate Institute of Electro-Optical Engineering
論文出版年：	2007
畢業學年度：	95
語文別：	中文
論文頁數：	66
中文關鍵詞：	散射係數、非均向性係數、相位延遲角、快軸夾角
英文關鍵詞：	scattering coefficient, anisotropy factor, phase retardation angle, fast axis angle
論文種類：	學術論文
相關次數：	點閱：212 下載：0
分享至:	分享至facebook 分享至twitter

查詢本校圖書館目錄查詢臺灣博碩士論文知識加值系統勘誤回報

在本研究中，我們開發了一套演算法，可從PS-OCT所量測到的二維影像中取得四個不同的光學參數，包括散射係數、非均向性係數、雙折射係數，以及快軸夾角。
　　散射係數與非均向性係數可用來描述樣品的散射效應和非均向性；雙折射特性指的是組織中快、慢軸折射率的差異；快軸夾角可以描述組織的纖維結構排列方向。首先，我們使用了一些仿體對演算法進行過測試與校正後，接著我們證明了使用這個方法可以量化的描述人體血管在正常與不同病變類型情況下的光學特性。此外我們也詳細討論了在不同組織中的雙折射特性。

In this study, we developed an algorithm to extract four different optical parameters including scattering coefficient, anisotropy factor, birefringence coefficient, and fast axis angle from polarization-sensitive optical coherence tomography (PS-OCT) images.
Scattering coefficient and anisotropy factor can be used to describe scattering effect and anisotropy characteristic of the sample. Birefringence property represents the difference in tissue refractive index between the birefringent fast and slow axes, while fast axis angle can describe the fibrous arrangement of the fibrous structure in the tissue. First, we use phantom to test and correct our algorithm in this study. And then, we demonstrate that the optical characteristic of normal and different kinds of lesions in human vessel can be quantitative described by this method. In addition, we also discuss the birefringence properties of several different tissues in detail.

目錄

中文摘要----------------------------------------------------------------------------I
英文摘要---------------------------------------------------------------------------II
目錄--------------------------------------------------------------------------------III
圖目錄索引-----------------------------------------------------------------------V
表目錄索引--------------------------------------------------------------------VIII

第一章    緒論-------------------------------------------------------------------- 1
1.1    研究動機與目的----------------------------------------------------------- 1
1.2    文獻回顧-------------------------------------------------------------------- 2
     1.2.1  光學同調斷層攝影術(OCT) ---------------------------------- 2
     1.2.2  極化光學同調斷層攝影術(PS-OCT) ------------------------ 4
     1.2.3  光學特性量測---------------------------------------------------- 5
     1.2.4  OCT散射模型--------------------------------------------------- 6
1.3    論文架構-------------------------------------------------------------------- 7

第二章    理論背景-------------------------------------------------------------- 8
2.1    　散射效應------------------------------------------------------------------- 8
     2.1.1  散射係數---------------------------------------------------------- 9
     2.1.2  散射相位函數與非均向性係數----------------------------- 10
2.2  極化與雙折射------------------------------------------------------------ 12
     2.2.1  極化-------------------------------------------------------------- 12
     2.2.2  雙折射----------------------------------------------------------- 14
第三章    實驗器材、原理與方法-------------------------------------------- 17
3.1    實驗器材與系統--------------------------------------------------------- 17
     3.1.1  光源-------------------------------------------------------------- 17
     3.1.2  PS-OCT系統架構---------------------------------------------- 19
     3.1.3  仿體備製-------------------------------------------------------- 20
3.2    數學模型------------------------------------------------------------------ 22
     3.2.1　Extended Huygens-Fresenel model-------------------------- 22
     3.2.2　散射效應下的Extended Huygens-Fresenel model-------- 24
3.3    相位延遲角與快軸夾角計算方式------------------------------------ 26
3.4  實驗方法------------------------------------------------------------------ 27
3.5  演算法流程--------------------------------------------------------------- 29

第四章    實驗結果與討論---------------------------------------------------- 31
4.1    系統校正與仿體測試--------------------------------------------------- 31
     4.1.1  縱向解析度與雙折射參數----------------------------------- 31
     4.1.2  單次散射與多重散射----------------------------------------- 32
     4.1.3  散射係數與非均向性係數----------------------------------- 33
     4.1.4  相位延遲與快軸夾角----------------------------------------- 37
4.2  生物組織樣品量測------------------------------------------------------ 39
     4.2.1  動脈硬化血管檢體-------------------------------------------- 39
         4.2.1.1  動脈硬化血管檢體PS-OCT與組織切片影像---- 39
         4.2.1.2  動脈硬化血管檢體光學參數實驗結果------------ 48
         4.2.1.3　光學參數相互關係分析------------------------------ 54
         4.2.1.4  討論------------------------------------------------------ 60

第五章    結論結論與未來展望---------------------------------------------- 61
5.1    結論------------------------------------------------------------------------ 61
5.2    未來展望------------------------------------------------------------------ 62

參考文獻--------------------------------------------------------------------------63

圖目錄索引

圖1-1    OCT系統架構------------------------------------------------------ 2
圖2-1    散射係數示意圖---------------------------------------------------- 9
圖2.2    散射示意圖--------------------------------------------------------- 10
圖2.3    Henyey-Greenstein函數模擬結果------------------------------ 11
圖2.4    (a)線性偏極化光-------------------------------------------------- 12
         (b)圓偏極化光----------------------------------------------------- 12
         (c)橢圓偏極化光-------------------------------------------------- 12
圖2.5    快軸夾角示意圖--------------------------------------------------- 15
圖3.1    發光二極體光源功率頻譜圖------------------------------------ 18
圖3.2    極化光學同調斷層攝影系統架構圖--------------------------- 20
圖3.3    Berek’s極化補償器(New Focus，Model 5540)------------- 20
圖3.4    intralipid-10% (500ml，Fresenius Kabi AB，Uppsala，Sweden)
         ----------------------------------------------------------------------- 21
圖3.5    Polystyrene microsphere
         (a)2.6% solids Latex，averageddiameter ≒ 0.992m------- 21
         (b)2.6% solids Latex，averaged diameter ≒ 0.465m------ 21
圖3.6    OCT系統樣品端幾何結構圖[修改自reference 29]--------- 23
圖3.7    實驗流程圖--------------------------------------------------------- 28
圖3.8    演算法流程圖------------------------------------------------------ 30
圖4.1    縱向解析度測試圖------------------------------------------------ 31
圖4.2    單次與多重散射的比較
         (a) 3% intralipid--------------------------------------------------- 33
         (b) 10% intralipid-------------------------------------------------- 33圖4.3    (a)相位延遲角測試圖-------------------------------------------- 37
         (b)快軸夾角測試圖----------------------------------------------- 37
圖4.4    具有不同病變的心血管組織------------------------------------ 39
圖4.5    正常血管組織
         (a)、(b)為四歲兒童的動脈血管組織--------------------------- 41
         (c)為30歲成人動脈血管組織---------------------------------- 42
         (d)~(f)為54歲捐心者動脈血管組織-------------------------- 42
圖4.6    鈣化的血管組織
         (a)~(d)--------------------------------------------------------------- 43
         (e)與圖4.9 (b)~(d)對應的組織切片圖------------------------44
         (f)---------------------------------------------------------------------44
圖4.7    纖維化血管壁(a)~(d)--------------------------------------------- 45
圖4.8    脂肪堆積的血管組織
         (a)~(d)--------------------------------------------------------------- 46
         (e)~(h)--------------------------------------------------------------- 46
圖4.9    不同類型組織的散射係數分佈
         (a)正常組織(b)鈣化組織(c)纖維化血管壁(d)脂肪堆積---- 53
圖4.10   不同類型組織的非均向性係數分佈
         (a)正常組織(b)鈣化組織(c)纖維化血管壁(d)脂肪堆積---- 53
圖4.11   不同類型組織的雙折射係數分佈
         (a)正常組織(b)鈣化組織(c)纖維化血管壁(d)脂肪堆積---- 54
圖4.12   不同類型組織的快軸夾角分佈
         (a)正常組織(b)鈣化組織(c)纖維化血管壁(d)脂肪堆積---- 54
圖4.13   光學參數關係圖
         (a)散射係數與非均向性係數關係圖-------------------------- 56
         (b)散射係數與雙折射係數關係圖----------------------------- 56
         (c)散射係數與快軸夾角關係圖-------------------------------- 57
         (d)非均向性係數關係圖與雙折射係數關係圖-------------- 57
         (e)非均向性係數關係圖與快軸夾角關係圖----------------- 58
         (f)雙折射係數與快軸夾角關係圖----------------------------- 58
圖4.14   固定快軸時，系統的快軸與相位延遲量測值關係圖------- 60

表目錄索引

表3.1    光源規格------------------------------------------------------------ 17
表4.1    不同濃度intralipid對應約化散射係數變動結果------------ 35
表4.2    不同濃度Polystyrene microsphere對應散射係數和非均向性
         係數變動結果----------------------------------------------------- 35
表4.3    不同粒徑的散射粒子對非均向性係數的影響--------------- 36
表4.4    正常組織光學參數統計表--------------------------------------- 48
表4.5    鈣化組織光學參數統計表--------------------------------------- 49
表4.6    纖維化組織光學參數統計表------------------------------------ 50
表4.7    脂肪堆積的血管組織光學參數統計表------------------------ 51
表4.8    散射係數，非均向性係數，雙折射係數與快軸夾角之相關
         係數比較----------------------------------------------------------- 59
                                

[1] 中華民國行政院衛生署衛生統計資訊網, (http://www.doh.gov.tw/statistic/index.htm).
[2] M. R. Hee, D. Huang, E. A. Swanson, and J. G. Fujimoto, "Polarization-sensitive low-coherence reflectometer for birefringence characterization and ranging," Opt. Soc. Am. B, vol. 9, 1992.
[3] C. K. Hitzenberger and A. F. Fercher, "Differentialphase contrast in OCT," Optics Letters, vol. 24, pp. 622-624, 1999.
[4] J. M. Schmitt, "Array detection for speckle reduction in OCM," Phys. Med. Biol., vol. 42, pp. 1427-1439, 1997.
[5] Z. P. Chen, Y. H. Zhao, S. M. Srinival, J. S. Nelson, N. Prakash, and R. D. Frostig, IEEE J. Selec. Topics Quant. Electro, vol. 5, p. 1134, 1999.
[6] J. Schmitt, "OCT elastography: imaging microscopic deformation and strain of tissue," Optics Express, vol. 3, pp. 199-211, 1998.
[7] R. Leitgeb, M. Wojtkowski, A. Kowalczyk, C. K. Hitzenberger, M. Sticker, and A. F. Fercher, "Spectral measurement of absorption by spectroscopic FDOCT," Optics Letters, vol. 25, pp. 820-822, 2000.
[8] W. WATANABE and K. ITOH, "Coherence Spectrotomography: Optical Spectroscopic Tomography with Low-Coherence Interferometry," OPTICAL REVIEW, vol. 7, pp. 406-414, 2000.
[9] M. Sticker, C. K. Hitzenberger, R. Leitgeb, and A. F. Fercher, "Quantitative differential phase measurement and imaging in transparent and turbid media by OCT," Optics Letters, vol. 26, pp. 518-520, 2001.
[10] J.-L. Boulnois, "Photophysical processes in recent medical laser developments: A review " Lasers in Medical Science, vol. 1, pp. 47-66, 1985.
[11] L. Thrane, H. T. Yura, and P. E. Andersen, "Analysis of optical coherence tomography systems based on the extended Huygens–Fresnel principle," J. Opt. Soc. Am. A/, vol. 17, 2000.
[12] J. M. Schmitt, A. Knuttel, M. Yadlowsky, and M. A. Eckhaus, "OCT of a dense tissue: statistics of attenuation and backscattering," Phys. Med. Biol., vol. 39, pp. 1705-1720, 1994.
[13] M. J. Yadlowsky, J. M. Schmitt, and R. F. Bonner, "Multiple scattering in optical coherence microscopy," Applied Optics, vol. 34, pp. 5699-5707, 1995.
[14] L. S. Dolin, "A theory of optical coherence tomography," Radiophys Quant Electr, vol. 41, pp. 850-873, 1998.
[15] Y. Pan, R. Birngruber, and R. Engelhardt, "Contrast limits of coherence-gated imaging in scattering media " Applied Optics, vol. 36, pp. 2979-2983, 1997.
[16] D. J. Smithies, T. Lindmo, Z. Chen, J. S. Nelson, and T. E. Milner, "Signal attenuation and localization in OCT studied by Monte Carlo simulation " Phys. Med. Biol., vol. 43, pp. 3025-3044, 1998.
[17] J. M. Schmitt and A. Knuttel, "Model of OCT of heterogeneous tissue " J. Opt. Soc. Am. A, vol. 14, pp. 1231-1242, 1997.
[18] J. M. Schmitt, A. Knuttel, and R. F. Bonner, "Measurement of optical properties of biological tissues by low-coherence reflectometry," Applied Optics, vol. 32, pp. 6032-6042, 1993.
[19] V. Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis. Bellingham: SPIE Press, 2000.
[20] I. Dror, A. Sandrov, and N. S. Kopeika, "Experimental Investigation of the Influence of the Relative Position of the Scattering Layer on Image Quality: the Shower Curtain Effect " Appl. Opt. , vol. 37, pp. 6495-6499, 1998.
[21] H. T. Yura, "Signal-to-noise Ratio of Heterodyne Lidar Systems in the Presence of Atmospheric Turbulence " Opt. Acta., vol. 26, pp. 627-644, 1979.
[22] "Oregon Medical Laser Center website, http://omlc.ogi.edu/."
[23] D. J. Griffiths, Introduction to Electrodynamics. New Jersey: Prentice Hall, 1999.
[24] A. R. M. A and B. N. M, "Ellipsometry and Polarized Light," n. ed, Ed. New York, 1979, p. 75.
[25] A. F. Fercher, "Optical coherence tomography," Journal of Biomedical Optics, vol. 1, pp. 157-176, 1996.
[26] 劉振軒, 何逸儒, 張文發, 祝平次, 王琇真, 組織病理染色技術與圖鑑: 台灣養豬科學研究所, 民85.
[27] K. Schoenenberger, B. W. Colston, D. J. Maitland, L. B. D. Silva, and M. J. Everett, "Mapping of Birefringence and Thermal Damage in Tissue by use of PSOCT," Applied Optics, vol. 37, pp. 6026-6036, 1998.
[28] 國立中央大學教學網,”http://webclass.ncu.edu.tw”.
[29] M. Autiero, R. Liuzzi, P. Riccio, and G. Roberti, "Determination of the concentration scaling law of the scattering coefficient of water solutions of Intralipid at 832 nm by comparison between collimated detection measurements and Monte Carlo simulations," Lasers in Surgery and Medicine, vol. 36, pp. 414-422, 2005.
[30] G. J. Tearney, I.-K. Jang, and B. E. Bouma, "Optical coherence tomography for imaging the vulnerable plaque," J Biomed Opt, vol. 11, p. 021002, 2005.
[31] W.-C. Kuo, N.-K. Chou, C. Chou, C.-M. Lai, H.-J. Huang, S.-S. Wang, and J.-J. Shyu, "Polarization-sensitive optical coherence tomography for imaging human atherosclerosis," Applied Optics, vol. 46, No.13, 2520-2527, 2007.
[32] A. M. Rollins, R. Ung-arunyawee, A. Chak, C. K. Wong, K. Kobayashi, M. V. Sivak, Jr., J. A. Izatt, and "Real-time in vivo imaging of human gastrointestinalultrastructure by use of endoscopic optical coherence tomographywith a novel efficient interferometerdesign," Optics Letters, vol. 24, pp. 1358-1360, 1999.
[33] M. Pircher, E. Goetzinger, R. Leitgeb, and C. K. Hitzenberger, "Transversal phase resolved PSOCT," Phys. Med. Biol., vol. 49, pp. 1257-1263, 2004.

本全文未授權公開

簡易檢索 / 詳目顯示

相關論文