研究生: |
戴川義 Chuan-Yi Tai |
---|---|
論文名稱: |
光學影像技術於生物組織之研究 The study of optical imaging technique on biological tissues |
指導教授: |
李亞儒
Lee, Ya-Ju |
學位類別: |
碩士 Master |
系所名稱: |
光電工程研究所 Graduate Institute of Electro-Optical Engineering |
論文出版年: | 2011 |
畢業學年度: | 99 |
語文別: | 中文 |
論文頁數: | 50 |
中文關鍵詞: | 頻域式光學同調斷層攝影系統 、都普勒光學微血管攝影術 |
英文關鍵詞: | Spectral-domain optical coherence tomography system, Doppler optical micro-angiography |
論文種類: | 學術論文 |
相關次數: | 點閱:91 下載:8 |
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光學影像技術具有價格低廉、快速顯像、可攜性等優點。本研究可分為兩部分,首先,我們建立一套頻域式光學同調斷層攝影系統,用以取得生物組織的背向散射訊號。其利用同調光源於異質性之組織,藉由獲得組織其不同折射率之背向散射訊號,致使重建組織之結構影像。
最後,我們有效利用都普勒光學微血管攝影術於仿體以及老鼠表皮血管分佈之成像。此外,並利用此技術於老鼠大腦之血管攝影實驗,使有效地成像出鼠腦之血管分佈影像。
Optical imaging technique has the advantages of cost-effectiveness, non-invasive, rapid imaging and portability.
In this thesis, we have two topics. Firstly, we developed a spectral-domain optical coherence tomography system to obtain biological tissue scattering, the system uses coherence gating of backscattered light for tomographic imaing of tissue structure. Variation in tissue scattering due to in-homogeneities in the optical index of refraction provide imaing contrast.
Finally, we have successfully used Doppler optical micro-angiography (DOMAG) to image phantom flow and skin blood perfusion in mice. We then conduct in vivo experiments on a mouse brain to demonstrate that DOMAG is capable of quantifying the blood flow within cerebrovascular network.
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[2]Joseph A. Izatt, Manish D. Kulkarni, Siavash Yazdanfar, Jennifer K. Barton, and Ashley J. Welch, “In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography,” OPTICS LETTERS, 22, 1439-1441, 1997.
[3]Yonghua Zhao, Zhongping Chen, Christopher Saxer, Shaohua Xiang, Johannes F. de Boer, and J. Stuart Nelson, “Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity, ” OPTICS LETTERS, 25, 114-116, 2000.
[4]Zhongping Chen, Thomas E. Milner, Shyam Srinivas, Xiaojun Wang, Arash Malekafzali, Martin J. C. van Gemert, and J. Stuart Nelson, “Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography,” OPTICS LETTERS, 22, 1119-1121, 2008.
[5]Ruikang K Wang, “Fourier domain optical coherence tomography achieves full range complex imaging in vivo by introducing a carrier frequency during scanning,” IOP PUBLISHING Ltd , PHYSICS MEDICINE BIOLOGY, 2007.
[6]Lin An, and Ruikang K. Wang, “Use of a scanner to modulate spatial interferograms for in vivo full-range Fourier-domain optical coherence tomography,” Optical Society of America, Vol. 32, No. 23, 2007.
[7]Ruikang K. Wang, “In vivo full range complex Fourier domain optical coherence tomography,” American Institute of Physics, 2007.
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[9]Lin An and Ruikang K Wang, “In vivo volumetric imaging of vascular perfusion within human retina and choroids with optical micro-angiography,” OPTICS EXPRESS 11438, Vol. 16, No. 15, 2008.
[10]Ruikang K Wang, and Lin An, “Doppler optical micro-angiography for volumetric imaging of vascular perfusion in vivo,” OPTICS EXPRESS 8926, Vol. 17, No. 11, 2009.
[11]Ruikang K. Wang, and Hrebesh M. Subhash, “Optical Microangiography High-Resolution 3-D Imaging of Blood Flow,” OPN Optics & Photonics News, 2009.
[12]Ruikang K. Wang, “Optical Microangiography: A Label-Free 3-D Imaging Technology to Visualize and Quantify Blood Circulations Within Tissue Beds In Vivo,” IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, Vol. 16, No. 3, 2010.
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[14]Lin An, and Ruikang K. Wang, “Depth resolved imaging of capillary networks in retina and choroid using ultrahigh sensitive optical microangiography,” Optical Society of America, Vol. 35, No. 9, 2010.
[15]Yali Jia An, and Ruikang K. Wang, “Label-free and highly sensitive optical imaging of detailed microcirculation within meninges and cortex in mice with the cranium left intact,” journal of Biomedical Optics, Vol. 15, 2010.
[16]Victor X.D. Yang, and I. Alex Vitkin, “Improved phase-resolved optical Doppler tomography using the Kasai velocity estimator and histogram segmentation,” Optics Communications, 209-124, 2002.
[17]Adrian Mariampillai, and Victor X. D. Yang, “Optimized speckle variance OCT imaging of microvasculature,” Optical Society of America, OPTICS LETTERS, Vol. 35, No. 8, 2010.
[18]Chuan Wang, and Yong Yang, “Monitoring of drug and stimulation induced cerebral blood flow velocity changes in rat sensory cortex using spectral domain Doppler optical coherence tomography,” journal of Biomedical Optics, Vol. 16(4), 2011.
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[20]Lingfeng Yu, and Zhongping Chen, “Doppler variance imaging for three-dimensional retina and choroid angiography,” journal of Biomedical Optics, Vol. 15(1), 2010.
[21]Jun Zhang, and Zhongping Chen, “In vivo blood flow imaging by a swept laser source based Fourier domain optical Doppler tomography,” OPTICS EXPRESS 7449, Vol. 13, No. 19, 2005.
[22]Shuichi Makita, and Yoshiaki Yasuno, “Optical coherence angiography,” OPTICS EXPRESS 7821, Vol. 14, No. 17, 2006.
[23]Yuankai K. Tao, and Joseph A. Izatt, “Single-pass volumetric bidirectional blood flow imaging spectral domain optical coherence tomography using a modified Hilbert transform,” OPTICS EXPRESS 12350, Vol. 16, No. 16, 2008.
[24]Dae Yu Kim, and Robert J. Zawadzki, “In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography,” BIOMEDICAL OPTICS EXPRESS 1504, Vol. 2, No. 6, 2011.
[25]Brian R. White, and Mark C. Pierce, “In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical Doppler tomography,” OPTICS EXPRESS 3490, Vol. 11, No. 25, 2003.
[27]Wolfgang Drexler, and James G. Fujimoto, “Optical Coherence Tomography Technology and Applications,” Berlin, Heidelberg : Springer Berlin Heidelberg, 2008.
[28]Tomasz Bajraszewski, Maciej Wojtkowski, Maciej Szkulmowski, Anna Szkulmowska, Robert Huber, and Andrzej Kowalczyk, “Improved spectral optical coherence tomography using optical frequency comb,” OPTICS EXPRESS 16, 4163-4176, 2008.
[29]S. R. Chinn, E. A. Swanson, and J. G. Fujimoto, “Optical coherence tomography using a frequency-tunable optical source,” OPTICS LETTERS, 22, 340-342, 2007.
[30]B. Golubovic, B. E. Bouma, G. J. Tearney, and J. G. Fujimoto, “Optical frequency-domain reflectometry using rapid wavelength tuning of a Cr4+:forsterite laser,” OPTICS LETTERS, 22, 1704-1706, 1997.
[31]The Warren Research Group at Duke University, http://www.chem.duke.edu/~wwarren/tissueimaging.php/
[32]A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, “Optical coherence tomography principles and applications, ” Rep. Prog. Phys., vol. 66, pp. 239-303,
2003