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研究生: 戴川義
Chuan-Yi Tai
論文名稱: 光學影像技術於生物組織之研究
The study of optical imaging technique on biological tissues
指導教授: 李亞儒
Lee, Ya-Ju
學位類別: 碩士
Master
系所名稱: 光電工程研究所
Graduate Institute of Electro-Optical Engineering
論文出版年: 2011
畢業學年度: 99
語文別: 中文
論文頁數: 50
中文關鍵詞: 頻域式光學同調斷層攝影系統都普勒光學微血管攝影術
英文關鍵詞: Spectral-domain optical coherence tomography system, Doppler optical micro-angiography
論文種類: 學術論文
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  • 光學影像技術具有價格低廉、快速顯像、可攜性等優點。本研究可分為兩部分,首先,我們建立一套頻域式光學同調斷層攝影系統,用以取得生物組織的背向散射訊號。其利用同調光源於異質性之組織,藉由獲得組織其不同折射率之背向散射訊號,致使重建組織之結構影像。
    最後,我們有效利用都普勒光學微血管攝影術於仿體以及老鼠表皮血管分佈之成像。此外,並利用此技術於老鼠大腦之血管攝影實驗,使有效地成像出鼠腦之血管分佈影像。

    Optical imaging technique has the advantages of cost-effectiveness, non-invasive, rapid imaging and portability.
    In this thesis, we have two topics. Firstly, we developed a spectral-domain optical coherence tomography system to obtain biological tissue scattering, the system uses coherence gating of backscattered light for tomographic imaing of tissue structure. Variation in tissue scattering due to in-homogeneities in the optical index of refraction provide imaing contrast.
    Finally, we have successfully used Doppler optical micro-angiography (DOMAG) to image phantom flow and skin blood perfusion in mice. We then conduct in vivo experiments on a mouse brain to demonstrate that DOMAG is capable of quantifying the blood flow within cerebrovascular network.

    摘要 I ABSTRACT II 表目錄 V 圖目錄 VI 第一章 緒論 1 1.1研究背景 1 1.2研究目的 1 第二章 理論基礎與文獻回顧 2 2.1光學同調斷層攝影術 2 2.1.1生物窗(Biological window) 4 2.1.2時域式光學同調斷層攝影術 5 2.1.3傅域式光學同調斷層攝影術 6 2.1.4物體空間掃描定義 8 2.2低同調干涉儀之成像理論 9 2.2.2低同調干涉頻譜特性 14 2.3 遠場光學之限制 16 2.4 視場參數計算 18 2.4.1共焦成像參數計算 18 2.4.2影像解析度 21 第三章 都普勒光學成像理論 22 3.1 都普勒效應 22 3.2 都普勒成像理論 25 3.3 都普勒光學成像技術 28 3.3.1 相位解析法 28 3.3.2 光斑變化測量成像法 30 3.3.3 都普勒光學微血管攝影術 31 第四章 實驗步驟與分析方法 33 4.1實驗架構 33 4.2 實驗材料與方法 35 4.2.1仿體之實驗材料與方法 35 4.2.2動物實驗之實驗材料與方法 36 4.2.2.1 老鼠表皮血管成像實驗步驟 36 4.2.2.2 老鼠大腦血管成像實驗步驟 37 4.3理論分析 38 4.3.1都普勒訊號擷取與血流成像 39 4.3.1血流影像與雜訊抑制 40 第五章 結果與討論 41 5.1 仿體實驗 41 5.1.1 仿體實驗之成像結果 41 5.2 生物組織實驗 42 5.2.1 老鼠表皮之成像結果 42 5.2.1 老鼠大腦之成像結果 44 第六章 結論與未來展望 46 6.1 結論 46 6.2 未來展望 46 參考文獻 47

    [1]Wolfgang Drexler and James G. Fujimoto, “Optical Coherence Tomography Technology and Applications” Berlin, Heidelberg : Springer Berlin Heidelberg, 2008.

    [2]Joseph A. Izatt, Manish D. Kulkarni, Siavash Yazdanfar, Jennifer K. Barton, and Ashley J. Welch, “In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography,” OPTICS LETTERS, 22, 1439-1441, 1997.

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    2003

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