安肌肽攝取對酸鹼緩衝能力與運動表現之影響
摘要
民國92年6月
研 究 生：林琮翔
指導教授：謝伸裕
前言：在高強度無氧性運動中，乳酸與氫離子的累積造成pH值過度下降，是引起肌肉疲勞現象的重要因素之一。本研究目的在於探討不同劑量安肌肽補充對於無氧性運動表現與血液酸鹼狀態的影響。方法：本研究以12名健康的體育系大學生為受試對象 (年齡22.3 ± 1.6歲、體重71.8 ± 10.8公斤、身高176.2 ± 3.9公分，最大攝氧量43.7 ± 8.2 ml/kg/min)，採雙盲重複量數實驗設計，並以對抗平衡次序法執行測驗。每位受試者均接受低劑量(L)15 mg/kg、高劑量(H)30 mg/kg以及安慰劑(P)之實驗處理，並在服用後進行125%最大攝氧量之跑步機衝刺跑(treadmill sprint run)測驗，觀察安肌肽對運動後血液酸鹼緩衝能力與運動衰竭時間的影響。運動前以留置針(21G)埋入肱前靜脈，以含有heparin之真空管在運動前及後2、5、10分鐘採集血液樣本，所有血液樣本立即以YSI 23L Plus分析血乳酸濃度，以Corning 240 pH meter分析血液pH值，並記錄TSR運動時間。結果：在不同劑量安肌肽間，血乳酸在運動後2、5、10分鐘並無顯著差異(p>.05)。但運動前血乳酸安靜值在高劑量處理 (1.14 ± 0.33 mM) 則顯著高於安慰劑處理 (0.84 ± 0.16 mM) (p<.05)，為減少此實驗誤差所造成的影響，以運動前安靜血乳酸值為前測共變數進行共變數分析，結果各劑量處理間在運動後2、5、10分鐘仍無顯著差異(p>.05)。運動後十分鐘之血液pH值，高劑量處理 (7.218 ± 0.091) 顯著高於安慰劑處理(7.115 ± 0.077) (p<0.05)。在血液緩衝能力上，高劑量處理 (49.51 ± 37.42 slykes) 顯著高於安慰劑處理 (24.59 ± 8.42 slykes) (p<0.05)。然而，TSR之衰竭時間在不同劑量處理間並無顯著差異(p>.05)。結論：運動前攝取安肌肽對高強度無氧性運動後血乳酸值以及運動衰竭時間並無影響，但高劑量安肌肽(30 mg/kg)的攝取可顯著提升運動中的血液酸鹼緩衝能力。
關鍵詞：安肌肽、酸鹼緩衝能力、運動表現、血乳酸、血液pH值

The Effects of Anserine Ingestion on
Buffering Capacity and Exercise Performance
June 2003 Abstract Student：Lin, Tsorng Shyang
Advisor：Hsieh, Shen-Yu
Introduction：During the high intensity anaerobic exercise, increased of lactic acid and hydrogen ion concentration can cause a decrease of pH in the muscle and blood, which is one of the factors that induces fatigue during muscle contraction. The purpose of the study is to examine the effects of different anserine dosages ingestion on anaerobic exercise performance and blood buffering capacity. Method：Twelve healthy college students (age：22.3 ± 1.6 years；weight：71.8 ± 10.8 kg；high：176.2 ± 3.9 cm； VO2max：43.7 ± 8.2 ml/kg/min) were the subjects. A repeated-measure and double-blind design was used and each subject went through all three treatments. The oral anserine ingestion for low (L) 15 mg/kg、high (H) 30 mg/kg dosages and placebo (P) condition was given in a counterbalanced order. One hour after ingestion, a 125% VO2max treadmill sprint run test was undertaken. The exercise time to exhaustion was recorded. Blood samples were drawn before exercise and 2,5,10 minutes after exercise from forearm vein with a 21G I.V. catheter placed in it and immediately analyzed for lactic acid concentration (YSI 23L Plus) and pH value (Corning 240 pH meter). The repeated-measure one-way ANOVA was used for statistical analysis to verify the differences in all variables between dosages. Results：The lactic acid concentrations at 2、5、10 minutes after exercise were not significant different between dosages (p>.05). However, a significant difference was found between H (1.14 ± 0.33 mM) and P group (0.84 ± 0.16 mM) before exercise (p<.05). Thus, a one-way ANCOVA was used to correct this experiment error. Result shows that there were still no significant differences between dosages on lactic acid concentrations at 2,5,10 minutes after exercise (p>.05). The blood pH at 10 minutes after exercise was significant different between H (7.218 ± 0.091) and P group (7.115 ± 0.077) (p<.05). The blood buffering capacity was significant different between H (49.51 ± 37.42 slykes) and P group (24.59 ± 8.42 slykes) (p<.05). The TSR exercise time to exhaustion was not significant different between dosages (p>.05). Conclusion：Ingestion of anserine with dosages used in present study had no effect on the lactic acid concentrations after high intensity anaerobic exercise and performance time to exhaustion. However, high dosage (30 mg/kg) ingestion of anserine can increase the buffering capacity of the blood during exercise.
Key words：anserine, buffering capacity, exercise performance, lactic acid, blood pH

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