研究生: |
蘇怡瑄 Su, Yi-Hsuan |
---|---|
論文名稱: |
利用SWATH及iTRAQ技術進行小鼠腎臟中塑膠微粒毒性之差異蛋白質體學分析 Differential Proteomic Analysis of Microplastic Toxicity in Mouse Kidney by SWATH and iTRAQ Technology |
指導教授: |
陳頌方
Chen, Sung-Fang |
口試委員: |
陳頌方
Chen, Sung-Fang 蕭伊倫 Hsiao, I-Lun 陳百昇 Chen, Pai-Sheng 梁恭豪 Liang, Kung-Hao |
口試日期: | 2023/07/17 |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2023 |
畢業學年度: | 111 |
語文別: | 中文 |
論文頁數: | 107 |
中文關鍵詞: | 塑膠微粒 、SWATH 、iTRAQ 、蛋白質體學 |
英文關鍵詞: | microplastic, SWATH, iTRAQ, proteomics |
研究方法: | 實驗設計法 |
DOI URL: | http://doi.org/10.6345/NTNU202301090 |
論文種類: | 學術論文 |
相關次數: | 點閱:68 下載:0 |
分享至: |
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報 |
根據聯合國的數據,每年有超過800萬噸的塑料垃圾被丟棄於海洋。這些塑膠垃圾會逐漸分解成塑膠微粒,最終可能積聚在人類食物鏈中。已有相關研究指出,塑膠微粒會積累在肝臟、腎臟和腸道等器官中。本研究使用了SWATH和iTRAQ兩種差異蛋白質技術,分析塑膠微粒投餵組和對照組的蛋白質體學。為了減少樣品的複雜度,腎臟蛋白質樣品經胰蛋白酶水解過後的胜肽,先經鹼性逆相層析法分離,接著進行奈米液相層析串聯質譜分析(nanoLC-MS/MS)。SWATH和iTRAQ在相對定量方面具有相似的趨勢,並分別成功定量了1984和2162個蛋白質,塑膠微粒投餵組和對照組之間有顯著差異的蛋白質有489個。透過GO及Metascape分析,發現這些差異表達的蛋白質主要與細胞過程、代謝過程和生物調節途徑有關。未來這些蛋白質有望成為塑膠微粒引起的腎損傷的潛在生物標記物,這項研究為塑膠微粒誘導腎損傷的機制提供新的見解。
According to the United Nations, more than 8 million tons of plastic waste are discarded in ocean every year. It will be physically or chemically decomposed into microplastics, which may eventually accumulate in the human food chain. It has been reported that it will accumulate in organs such as liver, kidney and intestine. Two differential proteomic technologies, SWATH and iTRAQ, were used to investigate protein profiles in microplastic-treated groups and control group in this study. To reduce sample complexity, the proteins from kidney samples were tryptic digested and fractionated by alkaline reverse phase chromatography, followed by nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The SWATH and the iTRAQ gave similar analytical performance. Overall, 1984 and 2162 proteins were successfully quantified by the SWATH and the iTRAQ, respectively. Microplastic-treated groups showed significant differences in 489 proteins treated and control groups. Based on the Gene Ontology and Metascape analysis, these differential expressed proteins were found to be mainly related to cellular process, metabolic process, and biological regulation pathways. These proteins are expected to serve as potential biomarkers of microplastic-induced kidney injury.
Jung YS, Sampath V, Prunicki M, Aguilera J, Allen H, LaBeaud D, Veidis E, Barry M, Erny B, Patel L, Akdis C, Akdis M, Nadeau K., Characterization and regulation of microplastic pollution for protecting planetary and human health. Environ Pollut. 2022, 315, 120442.
Jiang B, Kauffman AE, Li L, McFee W, Cai B, Weinstein J, Lead JR, Chatterjee S, Scott GI, Xiao S. Health impacts of environmental contamination of micro- and nanoplastics: a review. Environ Health Prev Med. 2020, 25, 29.
Yang X, Man YB, Wong MH, Owen RB, Chow KL. Environmental health impacts of microplastics exposure on structural organization levels in the human body. Sci Total Environ. 2022, 825, 154025.
Chang X, Xue Y, Li J, Zou L, Tang M. Potential health impact of environmental micro- and nanoplastics pollution. J Appl Toxicol. 2020, 40(1), 4-15.
Kiran BR, Kopperi H, Venkata Mohan S. Micro/nano-plastics occurrence, identification, risk analysis and mitigation: challenges and perspectives. Rev Environ Sci Biotechnol. 2022, 21(1), 169-203.
Mao X, Xu Y, Cheng Z, Yang Y, Guan Z, Jiang L, Tang K. The impact of microplastic pollution on ecological environment: a review. Front Biosci. 2022, 27(2), 46.
Bhatt P, Pathak VM, Bagheri AR, Bilal M. Microplastic contaminants in the aqueous environment, fate, toxicity consequences, and remediation strategies. Environ Res. 2021, 200, 111762.
Bai Z, Wang N, Wang M. Effects of microplastics on marine copepods. Ecotoxicol Environ Saf. 2021, 217, 112243.
Dong X, Liu X, Hou Q, Wang Z. From natural environment to animal tissues: A review of microplastics(nanoplastics) translocation and hazards studies. Sci Total Environ. 2023, 855, 158686.
Masiá P, Mateo JL, Arias A, Bartolomé M, Blanco C, Erzini K, Le Loc'h F, Mve Beh JH, Power D, Rodriguez N, Schaal G, Machado-Schiaffino G, Garcia-Vazquez E. Potential microplastics impacts on African fishing resources. Sci Total Environ. 2022, 806(Pt 2), 150671.
Zolotova N, Kosyreva A, Dzhalilova D, Fokichev N, Makarova O. Harmful effects of the microplastic pollution on animal health: a literature review. PeerJ. 2022, 10, e13503.
Zhang Q, Xu EG, Li J, Chen Q, Ma L, Zeng EY, Shi H. A Review of Microplastics in Table Salt, Drinking Water, and Air: Direct Human Exposure. Environ Sci Technol. 2020, 54(7), 3740-3751.
Jaikumar G, Brun NR, Vijver MG, Bosker T. Reproductive toxicity of primary and secondary microplastics to three cladocerans during chronic exposure. Environ Pollut. 2019, 249, 638-646.
Siddiqui SA, Singh S, Bahmid NA, Shyu DJH, Domínguez R, Lorenzo JM, Pereira JAM, Câmara JS. Polystyrene microplastic particles in the food chain: Characteristics and toxicity. A review. Sci Total Environ. 2023, 164531.
Andrady AL. Microplastics in the marine environment. Mar Pollut Bull. 2011, 62(8), 1596-605.
De-la-Torre GE. Microplastics: an emerging threat to food security and human health. J Food Sci Technol. 2020, 57(5), 1601-1608.
E.V. Sebille, C. Wilcox, L. Lebreton, N. Maximenko, B.D. Hardesty, J.A. van Franeker, M. Eriksen, D. Siegel, F. Galgani, K.L. Law, A global inventory of small floating plastic debris. Environ. Res. Lett. 2015, 10, 124006.
R.W. Obbard, S. Sadri, Y.Q. Wong, A.A. Khitun, I. Baker, R.C. Thompson, Global warming releases microplastic legacy frozen in Arctic Sea ice. Earth’s Fut. 2014, 2, 315-320.
Dehghani S, Moore F, Akhbarizadeh R. Microplastic pollution in deposited urban dust, Tehran metropolis, Iran. Environ Sci Pollut Res Int. 2017, 24(25), 20360-20371.
Xiang Y, Jiang L, Zhou Y, Luo Z, Zhi D, Yang J, Lam SS. Microplastics and environmental pollutants: Key interaction and toxicology in aquatic and soil environments. J Hazard Mater. 2022, 422, 126843.
Yuan Z, Nag R, Cummins E. Human health concerns regarding microplastics in the aquatic environment - From marine to food systems. Sci Total Environ. 2022, 823, 153730.
Goswami P, Selvakumar N, Verma P, Saha M, Suneel V, Vinithkumar NV, Dharani G, Rathore C, Nayak J. Microplastic intrusion into the zooplankton, the base of the marine food chain: Evidence from the Arabian Sea, Indian Ocean. Sci Total Environ. 2023, 864, 160876.
Huang D, Zhang Y, Long J, Yang X, Bao L, Yang Z, Wu B, Si R, Zhao W, Peng C, Wang A, Yan D. Polystyrene microplastic exposure induces insulin resistance in mice via dysbacteriosis and pro-inflammation. Sci Total Environ. 2022, 838(Pt 1), 155937.
Deng Y, Zhang Y, Lemos B, Ren H. Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure. Sci Rep. 2017, 7, 46687.
Meng X, Zhang J, Wang W, Gonzalez-Gil G, Vrouwenvelder JS, Li Z. Effects of nano- and microplastics on kidney: Physicochemical properties, bioaccumulation, oxidative stress and immunoreaction. Chemosphere. 2022, 288(Pt 3), 132631.
Chen YC, Chen KF, Lin KA, Chen JK, Jiang XY, Lin CH. The nephrotoxic potential of polystyrene microplastics at realistic environmental concentrations. J Hazard Mater. 2022, 427, 127871.
Ragusa A, Svelato A, Santacroce C, Catalano P, Notarstefano V, Carnevali O, Papa F, Rongioletti MCA, Baiocco F, Draghi S, D'Amore E, Rinaldo D, Matta M, Giorgini E. Plasticenta: First evidence of microplastics in human placenta. Environ Int. 2021, 146, 106274.
S. Magni, C. Della Torre, G. Garrone, A. D’Amato, C.C. Parenti, A. Binelli. First evidence of protein modulation by polystyrene microplastics in a freshwater biological model. Environ. Pollut. 2019, 250, 407-415.
Lu Y, Zhang Y, Deng Y, Jiang W, Zhao Y, Geng J, Ding L, Ren H. Uptake and Accumulation of Polystyrene Microplastics in Zebrafish (Danio rerio) and Toxic Effects in Liver. Environ Sci Technol. 2016, 50(7), 4054-60.
C. Zhang, C.B. Jeong, J.S. Lee, D. Wang, M. Wang. Transgenerational proteome plasticity in resilience of a marine copepod in response to environmentally relevant concentrations of microplastics. Environ. Sci. Technol. 2019, 53, 8426-8436.
J.Y. Zheng, Z.L. Tan, J.Y. Wu, J. Liu, T. Yang, H. Yang Polystyrene microplastics aggravate acute pancreatitis in mice. Toxicology, 2023, 491, 153513.
Yang YF, Chen CY, Lu TH, Liao CM. Toxicity-based toxicokinetic/toxicodynamic assessment for bioaccumulation of polystyrene microplastics in mice. J Hazard Mater. 2019, 366, 703-713.
Jin H, Ma T, Sha X, Liu Z, Zhou Y, Meng X, Chen Y, Han X, Ding J. Polystyrene microplastics induced male reproductive toxicity in mice. J Hazard Mater. 2021, 401, 123430.
Wang YL, Lee YH, Hsu YH, Chiu IJ, Huang CC, Huang CC, Chia ZC, Lee CP, Lin YF, Chiu HW. The Kidney-Related Effects of Polystyrene Microplastics on Human Kidney Proximal Tubular Epithelial Cells HK-2 and Male C57BL/6 Mice. Environ Health Perspect. 2021, 129(5), 57003.
Cortese M, Gigliobianco MR, Magnoni F, Censi R, Di Martino PD. Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review. Molecules. 2020, 25(13), 3047.
Han Z, Chen LC. A Subtle Change in Nanoflow Rate Alters the Ionization Response As Revealed by Scanning Voltage ESI-MS. Anal Chem. 2022, 94(46), 16015-16022.
Tiwary S, Levy R, Gutenbrunner P, Salinas Soto F, Palaniappan KK, Deming L, Berndl M, Brant A, Cimermancic P, Cox J. High-quality MS/MS spectrum prediction for data-dependent and data-independent acquisition data analysis. Nat Methods. 2019, 16(6), 519-525.
Gillet LC, Navarro P, Tate S, Röst H, Selevsek N, Reiter L, Bonner R, Aebersold R. Targeted data extraction of the MS/MS spectra generated by data-independent acquisition: a new concept for consistent and accurate proteome analysis. Mol Cell Proteomics. 2012, 11(6), O111.016717.
Ludwig C, Gillet L, Rosenberger G, Amon S, Collins BC, Aebersold R. Data-independent acquisition-based SWATH-MS for quantitative proteomics: a tutorial. Mol Syst Biol. 2018, 14(8), e8126.
Florens L, Washburn MP. Proteomic analysis by multidimensional protein identification technology. Methods Mol Biol. 2006, 328, 159-75.
Brunelle JL, Green R. One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). Methods Enzymol. 2014, 541, 151-9.
Chen X, Sun Y, Zhang T, Shu L, Roepstorff P, Yang F. Quantitative Proteomics Using Isobaric Labeling: A Practical Guide. Genomics Proteomics Bioinformatics. 2021, 19(5), 689-706.
Lu L, Wan Z, Luo T, Fu Z, Jin Y. Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in mice. Sci Total Environ. 2018, 631, 449-458.
Evans C, Noirel J, Ow SY, Salim M, Pereira-Medrano AG, Couto N, Pandhal J, Smith D, Pham TK, Karunakaran E, Zou X, Biggs CA, Wright PC. An insight into iTRAQ: where do we stand now? Anal Bioanal Chem. 2012, 404(4), 1011-27.
Jylhä A, Nättinen J, Aapola U, Mikhailova A, Nykter M, Zhou L, Beuerman R, Uusitalo H. Comparison of iTRAQ and SWATH in a clinical study with multiple time points. Clin Proteomics. 2018, 15, 24.
Luo T, Wang C, Pan Z, Jin C, Fu Z, Jin Y. Maternal Polystyrene Microplastic Exposure during Gestation and Lactation Altered Metabolic Homeostasis in the Dams and Their F1 and F2 Offspring. Environ Sci Technol. 2019, 53(18), 10978-10992.
Okamura T, Hamaguchi M, Hasegawa Y, Hashimoto Y, Majima S, Senmaru T, Ushigome E, Nakanishi N, Asano M, Yamazaki M, Sasano R, Nakanishi Y, Seno H, Takano H, Fukui M. Oral Exposure to Polystyrene Microplastics of Mice on a Normal or High-Fat Diet and Intestinal and Metabolic Outcomes. Environ Health Perspect. 2023, 131(2), 27006.
Wang W, Guan J, Feng Y, Nie L, Xu Y, Xu H, Fu F. Polystyrene microplastics induced nephrotoxicity associated with oxidative stress, inflammation, and endoplasmic reticulum stress in juvenile rats. Front Nutr. 2023, 9, 1059660.
Xiong X, Gao L, Chen C, Zhu K, Luo P, Li L. The microplastics exposure induce the kidney injury in mice revealed by RNA-seq. Ecotoxicol Environ Saf. 2023, 256, 114821.
Hu M, Palić D. Micro- and nano-plastics activation of oxidative and inflammatory adverse outcome pathways. Redox Biol. 2020, 37, 101620.
Shi C, Han X, Guo W, Wu Q, Yang X, Wang Y, Tang G, Wang S, Wang Z, Liu Y, Li M, Lv M, Guo Y, Li Z, Li J, Shi J, Qu G, Jiang G. Disturbed Gut-Liver axis indicating oral exposure to polystyrene microplastic potentially increases the risk of insulin resistance. Environ Int. 2022, 164, 107273.
Jin Y, Lu L, Tu W, Luo T, Fu Z. Impacts of polystyrene microplastic on the gut barrier, microbiota and metabolism of mice. Sci Total Environ. 2019, 649, 308-317.
Zheng H, Wang J, Wei X, Chang L, Liu S. Proinflammatory properties and lipid disturbance of polystyrene microplastics in the livers of mice with acute colitis. Sci Total Environ. 2021, 750, 143085.
Deng Y, Zhang Y, Qiao R, Bonilla MM, Yang X, Ren H, Lemos B. Evidence that microplastics aggravate the toxicity of organophosphorus flame retardants in mice (Mus musculus). J Hazard Mater. 2018, 357, 348-354.
Jassal B, Matthews L, Viteri G, Gong C, Lorente P, Fabregat A, Sidiropoulos K, Cook J, Gillespie M, Haw R, Loney F, May B, Milacic M, Rothfels K, Sevilla C, Shamovsky V, Shorser S, Varusai T, Weiser J, Wu G, Stein L, Hermjakob H, D'Eustachio P. The reactome pathway knowledgebase. Nucleic Acids Res. 2020, 48(D1), 498-503.
Fabregat A, Korninger F, Viteri G, Sidiropoulos K, Marin-Garcia P, Ping P, Wu G, Stein L, D'Eustachio P, Hermjakob H. Reactome graph database: Efficient access to complex pathway data. PLoS Comput Biol. 2018, 14(1), e1005968.
Dowhan W. Molecular basis for membrane phospholipid diversity: why are there so many lipids? Annu Rev Biochem. 1997, 66, 199-232.
Vik A, Dalli J, Hansen TV. Recent advances in the chemistry and biology of anti-inflammatory and specialized pro-resolving mediators biosynthesized from n-3 docosapentaenoic acid. Bioorg Med Chem Lett. 2017, 27(11), 2259-2266.
Kolb H, Kempf K, Röhling M, Lenzen-Schulte M, Schloot NC, Martin S. Ketone bodies: from enemy to friend and guardian angel. BMC Med. 2021, 19(1), 313.
Chen G, Zhou G, Lotvola A, Granneman JG, Wang J. ABHD5 suppresses cancer cell anabolism through lipolysis-dependent activation of the AMPK/mTORC1 pathway. J Biol Chem. 2021, 296, 100104.
van Dierendonck XAMH, Vrieling F, Smeehuijzen L, Deng L, Boogaard JP, Croes CA, Temmerman L, Wetzels S, Biessen E, Kersten S, Stienstra R. Triglyceride breakdown from lipid droplets regulates the inflammatory response in macrophages. Proc Natl Acad Sci U S A. 2022, 119(12), e2114739119.
Levy M, Futerman AH. Mammalian ceramide synthases. IUBMB Life. 2010, 62(5), 347-356.
Hunt MC, Siponen MI, Alexson SE. The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism. Biochim Biophys Acta. 2012, 1822(9), 1397-410.
Payne AH, Hales DB. Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocr Rev. 2004, 25(6), 947-970.
Makhdoumi P, Karimi H, Khazaei M. Review on Metal-Based Nanoparticles: Role of Reactive Oxygen Species in Renal Toxicity. Chem Res Toxicol. 2020, 33(10), 2503-2514.