研究生: |
林怡吟 Lin, Yi-Yin |
---|---|
論文名稱: |
臺灣地區麝香百合族群遺傳結構之研究 The Population Genetic Structure of Lilium longiflorum( |
指導教授: |
黃生
Huang, Shong |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
畢業學年度: | 84 |
語文別: | 中文 |
論文頁數: | 66 |
中文關鍵詞: | 族群遺傳學 、麝香百合 、遺傳結構 、同功異構酵素 |
英文關鍵詞: | Population Genetics, Lilium Longiflorum, Genetic Structure, Isozyme |
論文種類: | 學術論文 |
相關次數: | 點閱:191 下載:0 |
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臺灣東海岸地區的麝香百合(Lilium longiflorum Thunb.)多生長於海岸
崖地、突出的舺角及石灰岩質的珊瑚礁上,其棲地特殊 且呈被分割狀態。
這樣一個呈現島嶼狀分佈的物種,其族群遺傳結構究屬何種模型,亟待實
驗研究。本研究即利用同功異構酵素凝膠電永法探討海岸麝香百合的族群
遺傳結構。在分析的13種同功異夠酵素中,共判讀了20個基因座,其中屬多
形性基因座者為13個。各族群的平均多形性基因座百分比為 35.38%, 各
個基因座的平均對偶基因數為 1.61個, 各個基因座的平均有效對偶基因
數為1.17個; 族群的異質結合度期望值(Hep)平均值為 0.142, 異質結合
度觀察值(Hop)平均為0.128,這些數值顯示麝香百合的族群 遺傳變異度豐
富。在遺傳變異的分佈上, 約有76% 的變異存在於族群內,24% 存在於族
群間。族群間的分佈指數Fst=0.254,基因交流 數值Nm=0.734(<1),此二者
表示族群間因基因流傳受到部份限制, 致呈高度的的遺傳分化。異配生殖
及鱗莖繁殖的現象, 促進了麝香百 合遺傳變異的保存, 而遺傳結構則是
天擇與基因流傳相互作用的結果, 唯基因流傳的效應更甚於天擇。
The population of Lilium longiflorum along eastern coast of
Taiwan always occurs in isolated habitats.The population genetic
structure of a species which is island distribution like L.
longiflorum is a good theme to study.Thirteen population
weresampled and genetic variation was investigated using starch
gel eletrophoresis.Genetic disversity was rich for the species(
Hes=0.191)as well as within population(Hep=0.142).Of the 20
allozyme loci examined,13(65%)were polymorphic,and on
average35.38% of the loci were polymorphic within population.The
mean number of allelesper locus was 1.61 and the mean effective
number of that was 1.17 within population.We found a high
proportion of total genetic variation (24%) among population and
a significant correlation (p<0.001) between genetic distance and
geographic distance.Gene flow and nature selection appear to
play major roles in the population structureof the species,while
gene folw is more influential than nature selection.
The population of Lilium longiflorum along eastern coast of