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研究生： 張智勝
Chih-Sheng, Jhang
論文名稱： 利用紙片電噴灑質譜快速篩選及毛細管電泳質譜測定唾液中含4-氯安非他命的開發與研究
Rapid screening and determination of 4-chloroamphtamine in saliva by paper spray-mass spectrometry and capillary electrophoresis-mass spectrometry
指導教授： 林震煌
Lin, Cheng-Huang
學位類別： 碩士
Master
系所名稱： 化學系
Department of Chemistry
論文出版年： 2012
畢業學年度： 100
語文別： 中文
論文頁數： 71
中文關鍵詞： 紙片電噴灑質譜 、毛細管電泳電噴灑質譜 、4-氯安非他命
英文關鍵詞： paper spray-mass spectrometry, capillary electrophoresis electrospray ionization-mass spectrometry, 4-chloro-amphetamine
論文種類： 學術論文
相關次數： 點閱：278  下載：8 
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    • 本實驗開發了一種新型藥物篩選系統，包括濾紙電噴霧質譜（PS-MS）和毛細管電泳電噴灑質譜法（CE-ESI-MS）的方法。本實驗系統可以很容易地切換的PS-MS快速篩選樣品或傳統CE-ESI-MS分離方法，並利用同時共享同一台質譜而獲得詳細的質譜信息。在PS-MS情況下，當用尖端為（15度）的濾紙時，從紙尖到質譜入口的最佳距離大約口為7.7 mm，而最佳化CE-ESI尖端的距離為13.5 mm。另外本實驗是使用4 -氯安非他命作為測試樣品，而PS-MS和CE-ESI-MS偵測極限經過測試後確定分別為0.1和0.25 ppm。以下是使用PS-MS和CE-ESI-MS和實驗條件下得到的結果的比較與說明。

    A novel drug-screening system, consisting of paper spray-mass spectrometry (PS-MS) and a capillary electrophoresis electrospray ionization-mass spectrometry (CE-ESI-MS) method was developed. This system can be easily switched either to PS-MS for rapidly screening samples or to the traditional CE-ESI-MS method for separation and to obtain detailed mass spectral information, while sharing the same mass spectrometer. In the former case, when a sharp (15º-tip) chromatography paper was used, the optimized distance from the paper tip to the mass inlet was 7.7 mm, whereas the optimized distance for the CE-ESI tip was ~ 13.5 mm. Using 4-chloro-amphetamine as a model compound, the limits of detection for PS-MS and CE-ESI-MS were determined to ~ 0.1 and 0.25 ppm, respectively. Comparisons of results obtained using PS-MS and CE-ESI-MS and the experimental conditions are described.

    中文摘要....................................................Ⅰ 英文摘要....................................................Ⅱ 目錄.......................................................Ⅲ 圖目錄.....................................................Ⅴ 表目錄.....................................................Ⅵ 第一章、緒論................................................1 1-1 研究目的.............................................1 1-2 分析物簡介...........................................2 1-2-1 鹵素安非他命結構圖..............................3 1-3 濫用藥物的檢驗技術.....................................4 1-4 生物檢體之檢驗分析.....................................8 第二章、分析方法與原理.......................................10 2-1 毛細管電泳電噴灑質譜法 (CE-ESI-MS).....................10 2-2 離子阱質量分析器簡介...................................12 2-3 紙片電噴灑質譜法 (PS-MS)..............................13 2-4 大氣壓基質輔助雷射脫附游離法 (AP-MALDI).................14 2-5 電噴灑輔助雷射脫附游離法 (ELDI)........................15 2-6 萃取方法............................................16 第三章、儀器、藥品及實驗方法..................................18 3-1 CE-ESI-MS結合PS-MS之實驗方法.........................18 3-1-1 自組式毛細管電泳質譜法 (CE-ESI-MS)..............18 3-1-2 紙片電噴灑游離法 (PS-MS).......................21 3-2 常見雷射脫附游離之實驗方法..............................24 3-2-1 大氣壓基質輔助雷射脫附質譜 (AP-MALDI-MS).............24 3-2-2 電噴灑輔助雷射脫附質譜 (ELDI-MS)....................27 3-2-3 真空噴灑沉積 (spray deposition)...................30 3-3 儀器及周邊設備列表....................................31 3-4 藥品列表............................................32 第四章、結果與討論..........................................34 4-1 自製鋼筆頭式PS-MS及CE-ESI-MS位置最佳化.................35 4-1-1 PS-MS位置校正圖..............................36 4-1-2 CE-ESI-MS位置校正圖..........................41 4-1-3 PS-MS及CE-ESI-MS最佳位置比較..................43 4-2 CE-ESI-MS分離鹵素安非他命............................45 4-3 鹵素安非他命之真實樣品應用.............................47 4-3-1 唾液中鹵素安非他命的偵測..............................47 4-3-2 真實樣品前處理......................................48 4-4 雷射脫附游離源偵測鹵素安非他命..........................51 4-5 各種游離源偵測鹵素安非他命之整理.........................63 第五章、結論...............................................65 參考文獻..................................................66

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