研究生: |
張承俊 Cheng-Chun Chang |
---|---|
論文名稱: |
奈米鑽石結合質譜技術分析醣蛋白 The Application of Nanodiamond and Mass Spectrometry in Glycoproteins Analysis |
指導教授: |
張煥正
Chang, Huan-Cheng |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
論文出版年: | 2009 |
畢業學年度: | 97 |
語文別: | 中文 |
論文頁數: | 58 |
中文關鍵詞: | 奈米鑽石 、質譜 |
論文種類: | 學術論文 |
相關次數: | 點閱:181 下載:0 |
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我們發展了一套方法,此方法可以加快、簡化在分析醣蛋白上中性醣醣基時的實驗流程,而且同時也可以增強在利用基質輔助雷射脫附游離飛行時間質譜儀分析醣類時醣類的訊號強度。
此方法包括了利用微波加熱輔助進行醣蛋白的酵素消化及切醣,接著利用酸洗過後含有羧基的奈米鑽石進行純化,將胜肽移除,最後將待測樣品溶液先與氫氧化鈉溶液混合,再與基質(DHB)混合,以抑制在質譜儀分析時未移除乾淨的胜肽及醣類接鉀([M+K]+)的離子訊號。
利用此方法所展現的優點,皆是我們使用基質輔助雷射脫附游離飛行時間質譜儀分析Ovalbumin和Ribonuclease B這兩種醣蛋白上所切下的N-linked的醣類當作樣品所得到的結果。
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