研究生: |
陳倩雯 Chen Chien Wen |
---|---|
論文名稱: |
加速溶劑萃取系統與高效能液相層析介面在分析土壤中多苯環芳香烴化合物之方法研究 Interfacing ASE and HPLC for the determination of PAH in soil |
指導教授: |
吳家誠
Wu, Jia-Cheng |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
畢業學年度: | 87 |
語文別: | 中文 |
論文頁數: | 143 |
中文關鍵詞: | 加速溶劑萃取 、氣相層析儀 、高效率液相層析儀 、氣相質譜儀 、多苯環芳香烴化合物 、土壤 、螢光 |
英文關鍵詞: | ASE, GC, HPLC, GC, PAH, soil, Fluorescence |
論文種類: | 學術論文 |
相關次數: | 點閱:268 下載:0 |
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摘要
快速有效、方便又省溶劑的加速溶液萃取法(accelerated solvent extraction, ASE),已經藉著氣相層析(GC)、高效率液相層析(HPLC)或是氣相層析質譜法(GC-MS)運用在分析有機樣品研究上,很多種揮發或不揮發的有機化合物像是殺蟲劑及多苯環芳香烴化合物(polycyclic aromatic hydrocarbons, PAHs)更容易用HPLC來分離,因此我們設計一套連線介面將ASE連線於HPLC,使得樣品前處理的方法能保有ASE的簡單方便等各項優點,並且同時保有HPLC對揮發及不揮發的樣品中待測物的解析度。
本實驗是將ASE連線HPLC,運用於分析土壤樣品中的PAHs,從結果中發現這個方法能夠維持著HPLC的高解析度以及ASE的優點,像是操作簡單、省溶劑及良好之萃取效果等,並且因為連線減少了人為因素所造成的誤差,增加實驗速率。另外也利用標準參考物質(QCS345)樣品的多次驗證,測出的結果皆在證書的範圍內,證明這個方法能簡單有效準確的檢測土壤中之PAHs含量。這個方法也實際運用在台灣各地土壤的分析工作上,結果顯示本方法對於真實樣品也是可行的。
Abstract
As a fast, convenient, and low solvent consuming extraction method, accelerated solvent extraction (ASE) technique has been studied for the analyses of organic compounds followed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). However, many classes of widely-used organic compounds today are semi- or nonvolatiles, such as pesticides, and polycyclic aromatic hydrocarbons (PAH). They are easily separated by high performance liquid chromatography (HPLC). Thus, it would be advantageous to interface ASE with an HPLC system to provide easy sample preparation as well as possess high resolving power for semi- and non-volatiles simultaneously.
A method was developed to couple an accelerated solvent extraction system (ASE) with high performance liquid chromatography (HPLC) for the analysis of PAH in soil samples. The resolution of HPLC is well maintained while the advantages of ASE, fast extraction, less solvent consumption and ease of operation, are well expressed. The precision and accuracy of this method are verified by a series of analyses of reference material (QCS345sample from ERA). And the real sample collected at different sites in Taiwan. The data shows that it is feasible to analyze real samples using this method.
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