研究生: |
張玲齡 Ling-Ling Chang |
---|---|
論文名稱: |
白背芒之族群遺傳結構研究 Study on the Population Genetic Structure of Miscanthus sinensis var. glaber (Nakai) J. Lee |
指導教授: |
黃生
Huang, Shong |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
畢業學年度: | 87 |
語文別: | 中文 |
論文頁數: | 61 |
中文關鍵詞: | 白背芒 、族群遺傳結構 、天擇 、基因流傳 、同功酵素 |
英文關鍵詞: | Miscanthus sinensis var. glaber (Nakai) J. Lee, population genetic structure, selection, gene flow, isozyme |
論文種類: | 學術論文 |
相關次數: | 點閱:362 下載:0 |
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摘要
白背芒 ( Miscanthus sinensis var. glaber (Nakai) J. Lee ) 分布在台灣中低海拔1200公尺以下的山區,屬於先驅性、演替早期的風媒物種。
本研究分別在台灣北、中、南三個地區採集十個族群382株白背芒,利用澱粉凝膠電泳法探討白背芒的族群遺傳結構,並在台灣東北部採集二個亞族群70株白背芒進行微棲地天擇效應的檢測。總共分析了九種酵素,十五個基因座,得到結果如下:( 1 ) 白背芒全體族群的異合度期望值 ( HE ) 平均為0.146,族群間的遺傳歧異度 ( GST ) 為0.147,都接近以風力傳播種子之物種的平均值,且其族群間有高度分化的情形 ( FST = 0.158 )。( 2 ) 根據基因流傳值 ( Nm = 1.33 ) 與特有基因的出現判斷,白背芒全體族群的基因交流受到阻礙。( 3 ) 在同一棲地的族群內,不同微環境的亞族群間有中度分化的現象 ( FST = 0.077 ) 證明了地區性的天擇力量對於白背芒族群遺傳結構的影響力。( 4 ) 北大武山為一封閉隔離的地區,且其白背芒族群與其他族群間的遺傳相似度低於Gottlieb ( 0.95 ) 及Thrope ( 0.96 ) 提出的種內族群平均遺傳相似度,故經由以上結果判斷,北大武山的白背芒可能是由地區性天擇作用下建立起來的隔離族群。
ABSTRACT
The perennial herbaceous Miscanthus sinensis var. glaber (Nakai) J. Lee is a pioneer and early successional status species that can be found in altitude less than 1200 m.
A total number of 382 samples of M. sinensis var. glaber from ten localities ranging from Northern, Central and Southern Taiwan were collected to determine their population genetic structure. In addition, 70 samples representing two subpopulations in Northeastern Taiwan were collected for studying the nature selection of microhabitats. Fifteen loci from nine isozymes by Biosys-II were obtained and the results showed that the mean expected value of the heterozygosity ( HE ) is 0.1459 and the proportion of the total diversity among populations ( GST ) is 0.147, that correlated with those of species which disperse seeds by wind. The gene flow rate ( Nm = 1.33 ) and the acquisition of private gene suggest that the gene flow of Miscanthus sinensis var. glaber is retarded. The middle genetic differentiation ( FST = 0.077 ) among the subpopulations in different microhabitate showed the major force that acts on the population genetic structure of M. sinensis var. glaber is local sellection. The mean population genetic similarity ( 0.91 ) between samples from the geographically isolated area, Pei-Ta-Wu Mountain, and those from othe populations collected from rest nine sites is lower than that proposed by Gottlieb ( 0.95 ) and Thrope ( 0.96 ). The results indicated that the population in Pei-Ta-Wu Mountain might have been confined by local selection.
參考文獻
王冰心 1994. 合歡山區玉山箭竹族群之類黃素變異及遺傳結構. 國立中興大學植物學系碩士論文.
李瑞宗 1985. 林口紅土台地之植物相調查與邊坡植被分析. 國立臺灣大學植物學研究所分類組.
李瑞宗 1995. 臺灣芒屬植物的族群與其在民族植物學上角色之研究. 國立臺灣大學植物學研究所博士論文.
周昌弘, 李瑞宗 1991. 陽明山國家公園芒草生態之研究. 內政部營建署陽明山國家公園管理處委託.
黃 生 1991. 基因流傳與族群分化. 生物科學 34(2): 19-32.
黃 生 1992. 基因浮動 --- 兼談稀有物種的保育. 生物科學 35(1): 1-7。
陳雅慈, 陳進霖, 林怡孜, 王國雄, 黃生 1998. 台灣東北部的矢竹亞族群間的遺傳變異度分析. 師大生物學報 33(1): 57-69.
楊靜芬 1998. 高山芒之族群遺傳結構研究. 國立台灣師範大學生物學系碩士論文.
張至善 1992. 北大武山針闊葉樹自然保護區鐵杉林林分組成與塊集構造之研究. 國立臺灣大學森林學系碩士論文.
Affre, L. and J. D. Thompson 1997. Variation in the population genetic structure of two Cyclamen species on the island of Corsica. Heredity 78(2): 205-214.
Antonovics, J. 1968. Evolution in closely adjacent plant populations. IV. Manifold effects of gene flow. Heredity 23: 507-524.
Beattie, A. 1978. Plant-animal interactions affecting gene flow in Viola. In. A. J. Kichards (ed.), The pollination of flowers by insects. pp. 151-164. Academic Press, Linnean Society Symposium Series, #6, London.
Beaulieu, J. and J. P. Simon 1995. Mating system in natural populations of eastern white pine in Quebec. Can. J. For. Res. 25: 1697-1703.
Bergmann, F. 1975. Adaptive acid phosphatase polymorphism in conifer seeds. Silvae Genetica 24: 5-6.
Booth, W. E. 1964. Agrostology. Ann. Arbor, Michigan: Edwards Brothers, Inc.
Brown, A. H. D. 1979. Enzyme polymorphism in plant populations. Theor. Pop. Biol.15: 1-42.
Brown, A. H. D. and B. S. Weir 1983. Measuring genetic variability in plant population. In Tanskiey, S. D. and T. J. Orton (eds.), Isozyme in plant genetics and breeding, pp. 219-239. Elsevier.
Broyles, S. B. and R. Wyatt 1993. Allozyme diversity and genetic structure in southern Appalachian populations of poke milkweed, Asclepias exaltata. Syst. Bot. 18(1): 18-30.
Chapman, G. P. 1996. The biology of grasses. pp. 1-14, 159-178. Biddles Ltd, Guildford.
Chen, S. H. and W. Kim 1997. Comparison of plant community structures in cut and uncut areas at burned area of Mt. Gumo-san. J. Korean For. Soc. 86(4): 509-520.
Chou, C. H. and Y. T. Chung 1974. Allelopathic potential of Miscanthus floridulus. Bot. Bull. Acad. Sin. 15: 14-27.
Chou, C. H. and F. C. Chang 1988. Population study of Miscanthus II. Ecotypic variation of M. floridulus and M. transmorrisonensis as affected by altitude in Nantou, Taiwan. Bot. Bull. Acad. Sin. 29: 301-314.
Chou,C. H. and Y. F. Lee 1991. Allelopathic dominance of Miscanthus transmorrisonensis in an Alpine grassland community in Taiwan. J. Chem. Ecol. 17(11): 2267-2282.
Chou, C. H., S. Y. Hwang, and F. C. Chang 1987. Population study of Miscanthus floridulus (Labill.) Warb. I. Variation of peroxidase and Esterase in 27 populations in Taiwan. Bot. Bull. Acad. Sin. 28: 247-281.
Chou, C. H., P. S. Lee, Y. C. Chiang, and T. Y. Chiang 1996. Molecular systematics and evolution of Miscanthus sinensis complex in Taiwan based on sequences of rDNA ITS region. (manuscript)
Chou, C. H., Y. F. Lee, C. Y. Chiu, Y. C. Wang, and F. H. Hsu 1991. Population study of Miscanthus IV. Growth performance of M. floridulus and M. transmorrisonensis and their acclimation to temperatures and water stress. Bot. Bull. Acad. Sin. 32: 87-96.
Clegg, M. T. 1980. Measuring plant mating systems. Bioscience 30 (12): 814-818.
Clegg, M. T. and R. W. Allard 1972. Patterns of genetic differentiation in the slender wild oat species Avena barbata. Proc. Nat. Acad. Sci. USA 69: 1820-1824.
Cole, C. T. and D. D. Biesboer 1992. Monomorphism, reduced gene flow, and cleistogamy in rare and common species of Lespedeza ( Fabaceae ). Amer. J. Bot. 79 (5): 567-575.
Connor, H. E. 1979. Breeding system in the grasses : a survey. New Zealand J. Bot. 17: 547-574.
Crow, J. F. and M. Kimura 1970. An introduction to population genetics theory. Harper and Row, New York.
Endler, J. A. 1973. Gene flow and population differentiation. Science 179: 243-250.
Feret, P. P. 1971. Isozyme variation in Picea glauca (Moench) Voss seedlings. Silvae Genetica 20: 46-50.
Futuyma, D. J. 1986. Evolutionary biology. Sinauer Associates, Inc. Sunderland,
Gottlieb, L. D. 1977. Electrophoretic evidence and plant systematics. Ann. Mo. Bot. Gard. 64: 161-180.
Gottlieb, L. D. 1982. Conservation and duplication of isozymes in plants. Science 216: 373-380.
Greef, J. M., M. Deuter, C. Jung, and J. Schondelmaier 1996. Genetic diversity of European Miscanthus species revealed by AFLP fingerprinting. J. Gen. Res. Crop Evol.
Hamrick, J. L. and L. R. Holden 1979. Influence of microhabitat heterogeneity on gene frequency distribution and gametic phase disequilibrium in Avena barbata. Evolution 33: 521-533.
Hamrick, J. L. and R. W. Allard 1972. Microgeographical variation in allozyme frequencies in Avena barbata. Evolution 33: 521-533.
Hamrick, J. L. and M. J. Godt 1989. Allozyme Diversity in Plant Species. In Brown, A. H. D. M. T. Clegg, A.L. Kahler, and B.S. Wier (eds.), Plant Population Genetics, Breeding and Genetic Resources. pp. 43-63. Sinauer Associates, Inc. Sunderland.
Hamrick, J. L., Y. B. Linhart, and J. B. Mitton 1979. Relationships between life history characteristic and electrophoretically detectable genetic variation in plants. Ann. Rev. Ecol. Syst. 10: 173-200.
Harper, J. L. 1977. Population Biology of Plants. Academic Press, New York.
Hartl, D. L. and A. G. Clark 1989. Principles of Population Genetics, 2nd. ed. Sinauer Associates, Inc. Sounderland.
Hartley, W. 1958. Studies on the origin, evolution, and distribution of Gramineae 1. The tribe Andropogoneae. Aust. J. Bot. 6: 116-128.
Hedrick, P. W. 1983. Genetics of populations. Science Books International, Inc. Boston.
Hedrick, P. W. 1984. Population biology : The evolution and ecology of populations. Jones and Bartlet, Inc. U.S.A.
Hedrick, P. W., M. E. Ginevan, and E. P. Ewing 1976. Genetic polymorphism in heterogeneous environments. Ann. Rev. Ecol. Syst. 7: 1-32.
Hermann, R. K. and D. P. Lavender 1969. Early growth of douglas-fir from various altitudes and aspects in southern Oregon. Genetica 17: 143-151.
Howe, H. F. and L. C. Westley 1986. Ecology of Pollination and Seed Dispersal. In Crawley, M. J. (ed.), Plant Ecology. pp. 188-215.
Hsu. F. H. and C. H. Chou 1992. Inhibitory effects of heavy metals on seed germination and seedling growth of Miscanthus species. Bot. Bull. Acad. Sin. 33: 335-342.
Johri, B. M. and I. K. Vasil 1961. Physiology of Pollen. Bot. Rev. 27(3): 325-381.
Kao, W. Y., T. T. Tsai, and W. H. Chen 1998. A comparative study of Miscanthus floridulus (Labill) Wrab and M. transmorrisonensis Hayata: photosynthetic gas exchange, leaf characteristics and growth in controlled environments. Ann. Bot. 81(2): 295-299.
Kephart, S. R. 1990. Starch gel electrophoresis of plant isozymes: a comparative analysis of techniques. Amer. J. Bot. 77: 693-712.
Krauss, S. L. 1994. Restricted gene flow within the morphologically complex species Persoonia mollis (Proteaceae): contrasting evidence from the mating system and pollen dispersal. Heredity 73(2): 142-154.
Land, R. and D. W. Schemske 1985. The evolution of self-fertilization and inbreeding depression in plants.I.genetic models. Evolution 39: 24-40.
Levin, D. A. 1981. Dispersal versus gene flow in plants. Ann. Mo. Bot. Gard. 68: 233-253.
Levin , D. A. and H. W. Kerster 1974. Gene flow in seed plants. Evol. Biol. 7: 139-220.
Li, C. C. and D. G. Horvitz 1953. Some methods of estimating the inbreeding coefficient. Amer. J. Hum. Genet. 5: 107-117.
Liengsiri, C., C. Pievoluang, and T. J. B. Boxle 1990. Starch gel electrophoresis of tropical trees, A manual. ASEAN-Canada Forest Tree Seed Center.
Loiselle, B. A., V. L. Sork, J. Nason, and C. Graham 1995. Spatial genetic structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae). Amer. J. Bot. 82: 1420-1425.
Loveless, M. D. and J. L. Hamrick 1984. Ecological determinants of genetic structure in plant population. Ann. Rev. Ecol. Syst. 15: 65-75.
Maruyama, T. and M. Nei 1981. Genetic variability maintained by mutation and overdominant selection in finite population. Genetics 98: 441-459.
Maynard Smith, J. 1996. The evolution of population. In Campbell. N. A. (ed.) Biology. 4th ed. pp. 442-443. The Benjamin/Cummings publishing company, Inc. Menlo Park, California.
Mayr, E. 1947. Ecological factors in speciation. Evolution 1: 263-288.
Mayr, E. 1993. Mechanisms of evolution. In Cambell, N. A. Biology. p.416. The Benjamin/Cummings. Publishing Compancy, Inc.
Mitton, J. B. 1992. The dynamic mating systems of conifers. New Forests 6: 197-216.
Mitton, J. B., Y. B. Linhart, J. L., J. L. Hamrick, and J. S. Beckman 1977. Observations on the genetic structure and mating system of ponderosa pine in Colorado front range. Theor. Appl. Genet. 51: 5-13.
Mitton, J. B., Y. B. Linhart, J. L., K. B. Sturgeon, and J. L. Hamrick 1979. Allozyme polymorphism detected in nature needle tissue of ponderosa pine. J. Hered. 70: 86-89.
Mitton, J. B., K. B. Sturgeon, and M. L. Davis 1980. Genetic differentiation in ponderosa pine along a steep elevation transect. Silvae Genetica 29: 100-103.
Mitton, J. B., H. P. Stutz, W. S. Schuster, and K. L. Shea. 1989. Genotypic differentiation at PGM in Engelmann spruce from wet and dry sites. Silvae Genetica 38: 217-221.
Moran, G. F. and W. T. Adams 1989. Microgeographical patterns of allozyme differentiation in douglas-fir from southwest Oregon. For. Sci. 35: 3-15.
Nei, M. 1972. Genetic distance between populations. Amer. Natur. 106: 283-292.
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci. USA. 70: 3312-3323.
Nei, M. 1977. F-statistics and analysis of gene diversity in subdivided populations. Ann. Hum. Genet. 47: 225-233.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
Nei, M. T. Maruyama and R. Chakraborty 1975. The bottlenect effect and genetic variability in populations. Evolution 29: 1-10.
Nielsen, P. N. 1987. Vegetative propagation of Miscanthus sinensis cultivar Giganteus. Tidsskrift for Planteavl 91(4): 361-368.
Nishiwaki, A., K. Sugawara, and I. Ito 1996. The effect of cattle grazing on seed production in Miscanthus sinensis Anderss. Grassland Sci. 42(1): 47-51.
Perez-Nasser, -N., Eguiarte, -L.E., Pinero, and -D. 1993. Mating system and genetic structure of the distylous tropical tree Psychotria faxlucens (Rubiaceae). Amer. J. Bot. 80(1): 45-52.
Perry, D. J. and P. Knowles 1990. Evidence of high self-fertilization in natural populations of eastern white cedar ( Thuja occidentalis ). Can. J. Bot. 68: 663-668.
Prentice, H. C., M. Lonn, L. P. Lefkovitch, and H. Runyeon 1995. Associations between allele frequencies in Festuca ovina and habitat variation in the alvar grasslands on the Baltic island of Oland. J. Ecol. 83(3): 391-402.
Ritland, K. and S. Jain 1981. A model for the estimation of outcrossing rate and gene frequencies using independent loci. Heredity 47(1): 35-52.
Schaal, B. A. 1975. Population structure and local differentiation in Liastris cylindracea. Amer. Nature 109: 511-528.
Schaal, B. A. 1980. Measurement of gene flow in Lupinus texensis. Nature 284: 450-451.
Schemsk, D. W. 1984. Population structure and local selection in Impatiens (Balsaminaceae ), a selfing annual. Evolution 38: 817-832.
Shin J. S., H. W. Lee, and D. A. Kim 1989. Studies on hilly pasture improvement by hoof cultivation: II. Effect of grazing intensity on newly sown grasses establishment and its survival at natural grasslands. Korean J. Animal Sci. 31(6): 408-411.
Slatkin, M. 1985. Gene flow in natural populations. Ann. Rev. Ecol. Syst. 16: 393-430.
Slatkin, M. 1987. Gene flow and the geographic structure of natural population. Science 236: 787-792.
Sneath, P. H. A. and R. R. Sokal 1973. Numerical Taxonomy. W. H. Freedman, San Francisco.
Soltis, D. E., C. H. Haufler, D. C. Darrow, and G. J. Gastony 1983. Starch gel electrophoresis of ferns:a compliation of grinding buffers, gel and electrode buffers, and staining schedules. Amer. Fern J. 73: 9-27.
Soltis, D. E. and P. S. Soltis 1989. Isozymes in Plant Biology. Dioscorides Press, Portland, Oregon.
Soltis, P. S., D. E. Soltis, and L. D. Gottlieb 1987. Phosphoglucomutase gene duplications in Clarkia (Onagraceae) and their phylogenetic implications. Evolution 41: 667-671.
Sugimoto Y., M. Hirata, M. Ueno, and I. Nikki 1987. Effect of slope aspect on the vegetational and soil characteristics: An investigation on Mt. Ono in Aso Province (Japan). Bull. Fac. Agr. Miyazaki Univ. 34(2): 297-304.
Swofford, D. L. and R. B. Selander 1996. Biosys-II. University of Illinois. Urbana, Illinois.
Thorpe, J. P. 1982. The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Ann. Rev. Ecol. Syst. 13: 139-168.
Whitehead D. R. 1983. Wind Pollination : Some Ecological and Evolutionary Perspectives. In Pollination Biology. pp. 97-107. Academic Press, Inc.
Wei, D. S., C. M. Kuo, and S. M. Pan 1997. Studies on the activity of superoxide dismutase in Miscanthus floridulus grown near a copper smelter in Taiwan. Taiwania 42(3): 189-220.
Wright, J. W. 1976. Introduction to forest genetics. Academic Press, New York.
Wright, S. 1931. Evolution in Mendelian populations. Genetics 16: 97-159.
Wright, S. 1943a. Isolation by distance. Genetics 28: 114-138.
Wright, S. 1943b. Analysis of local variability of flower color in Linanthus parryae. Genetics 28: 139-156.
Wright, S. 1946. Isolation by distance under diverse systems of mating. Genetics 31: 39-59.
Wright, S. 1951. The genetical structure of population. Ann. Eugen. 15: 323-354.
Wright, S. 1965. The interpretation of population structure by F- statistics with special regard to systems of mating. Evolution 19: 395-420.
Wright, S. 1978. Variability within and among natural populations. Evolution and the genetics of populations. Vol. 4. University of Chicago Press, Chicago.
Xie, C. Y., B. P. Dancik, and F. C. Yeh 1991b. The mating system in natural populations of Thuja orientalis. Can. J. For. Res