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| 研究生： |
許齊文 Chi-Wen Shiu |
|---|---|
| 論文名稱： |
磁減量生化檢測應用於 人類肝癌及大腸癌之檢驗特性研究 Immunomagnetic rednction of human Alpha-fetoprotein and carcinoembryonic Antigen |
| 指導教授： |
洪姮娥
Horng, Herng-Er 謝振傑 Chieh, Jen-Je |
| 學位類別： |
碩士 Master |
| 系所名稱： |
光電工程研究所 Graduate Institute of Electro-Optical Engineering |
| 論文出版年： | 2012 |
| 畢業學年度： | 100 |
| 語文別： | 中文 |
| 論文頁數： | 38 |
| 中文關鍵詞： | 磁減量 |
| 論文種類： | 學術論文 |
| 相關次數： | 點閱：74 下載：0 |
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在臨床診斷上約 60%~70% 的肝癌患者血清中Alpha-fetoprotein ( AFP ) 濃度較正常人來的高 ( AFP cut-off 值為 20 mg/ml )，適合作為篩檢肝癌的腫瘤指標蛋白 ( biomarker )。另外在臨床診斷上約50%~80%的大腸癌患者血清中Carcinoembryonic Antigen ( CEA ) 濃度較正常人來的高 ( CEA cut-off 值為 5 ng/ml )，適合作為篩檢大腸癌的 biomarker。然而現行普遍使用的免疫檢測如酵素連結免疫吸附分析 ( Enzyme-Link Immunosorbent Assay；ELISA ) 因其操作過程繁雜與易受待測樣品影響等種種問題，我們需要另尋一種更快速且準確的免疫檢測方法。
本實驗室已歷經使用Anti-CRP( C-reactive protein )與奈米磁性粒子結合，形成一具有磁性生物標記( magnetic biomarker ) 之磁性試劑( magnetic reagent )。再利用本實驗室所研發之磁減量檢測法( Immunomagnetic rednction ; IMR )，其靈敏度可達10-4 mg/ml，於是本研究中我們如法泡製將Anti-AFP 和Anti-CEA 分別與奈米磁性粒子結合成為AFP試劑和CEA試劑，並進行試劑長期穩定度觀察、干擾度測試，最後藉由IMR檢測人類血清檢體，以探討人類肝癌及大腸癌之檢驗特性。
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