研究生: |
高智亮 Chih-Liang Kao |
---|---|
論文名稱: |
混合價錯合物修飾電極在感測超氧陰離子的研究 Study on the detection of superoxide anion using mixed-valence complex modified electrode |
指導教授: |
林如章
Lin, Ru-Jang |
學位類別: |
碩士 Master |
系所名稱: |
化學系 Department of Chemistry |
畢業學年度: | 87 |
語文別: | 中文 |
中文關鍵詞: | 超氧陰離子 、混合價錯合物 、玻璃化碳電極 |
英文關鍵詞: | Superoxide anion radical, Mixed-valence complex, glass carbon electrode |
論文種類: | 學術論文 |
相關次數: | 點閱:253 下載:0 |
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超氧陰離子(superoxide redical, O2-)在生物體內具有殺菌的功能,但在超氧陰離子的濃度過高時,就會對身體的組織造成傷害。在環境上,超氧陰離子也被懷疑為海洋鞭毛藻類造成魚類大量死亡的原因。因此對超氧陰離子的濃度的感測便顯得重要。
本研究以開發非破壞性及高感度的超氧陰離子感測電極為目的。利用循環伏安法將混合價錯合物(mixed-valence complex)普魯士藍(Prussian Blue, PB)及ruthenium(II) hexacyanoferrate(RuHCF)修飾於玻璃化碳電極(GC)的表面,以感測超氧陰離子濃度。為了阻隔干擾物如維他命C(Ascoribic acid, AA)及尿酸(Uric acid, UA)等物質對感測訊號的影響,再以適當的電解聚合條件形成一層poly(o-phenylenediamine)(PPD)於電極修飾層之外。由此兩種修飾電極分別感測xanthine-xanthine oxidase(XOD)的超氧陰離子產生系統。結果發現PPD/PB/GC電極在感測電位定為370mV(vs. Ag/AgCl)時,雖然能除去UA的干擾,但是AA的干擾卻無法去除。而PPD/RuHCF/GC電極,在感測電位定在350mV(vs. Ag/AgCl)時,能夠避免UA及AA的干擾。這可能是PPD/PB/GC電極以high spin的鐵為反應中心,因具有較高的反應性,使得感測過程中易與AA起反應,而PPD/RuHCF/GC的反應中心為low spin的鐵,反應性較低,因此較能避免AA所造成的干擾。PPD/RuHCF/GC電極在xanthine濃度範圍為5*10-5M~9.5*10-4M之間有良好的線性(R2=0.9989),相對於超氧陰離子的濃度為2.9*10-7M~5.5*10-6M。
Superoxide anion radical (O2-) has received a great deal attention in the fields of biochemistry, medicine and environment. Mixed-valence complex (Prussian Blue and ruthenium(II) hexacyanoferrate ) modified electrodes are investigated for the detection of superoxide anion radical in this study. The superoxide anion radical amperometric sensors were made by poly(o-phenylenediamine) (PPD) electrodeposition onto Prussian Blue (PB) modified electrode and ruthenium(II) hexacyanoferrate (RuHCF) modified electrode. O2- was generated by Xanthine-Xanthine Oxidase system. The interference of ascoribic acid can be avoided with PPD/RuHCF/GC but PPD/PB/GC. The PPD/RuHCF/GC response exhibited a linear dependence on analytic concentration of xanthine in the range 5*10-5~9.5*10-4 M (O2- concentration 2.9*10-7~5.5*10-6 M).
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