大腸桿菌受到紫外光照射後,菌體形貌會產生變化,且隨著照射強度增加,菌體形貌的變化也會增加,利用原子力顯微鏡在生物樣品量測的優勢,來了解為什麼菌體會有如此變化。細胞壁是主要維持細胞形貌的因素,而組成細胞壁的結構分別是肽聚醣層與脂多醣層,所以探究肽聚醣層與脂多醣層是否遭受紫外光照射而破壞,是本研究的重點。
本研究利用原子力顯微鏡,在大氣環境下測得,大腸桿菌產生的吸附力做功會隨著照射紫外光的能量提升而下降,這表示維持吸附力的脂多醣層的確受到紫外光照射而破壞 在液態環境下測得,大腸桿菌的有效彈性常數與楊氏模數經由紫外光照射後會下降,利用楊氏模數下降這可以證明紫外光照射,也會產生肽聚醣的破壞。
使用恆定力輕敲式掃描模式系統進行實驗,利用其在液態環境優異的解析度,讓我們在液態環境更精準的量測,實驗結果,其吸附力與楊氏模數的下降,與傳統原子力顯微鏡的結果相同。
使用表面增強拉曼光譜進行實驗,可以發現受紫外光照射的大腸桿菌有許多來自於DNA的訊號,未照射紫外光的大腸桿菌則沒有DNA的訊號,這表示,細胞壁的確受到了損害。
關鍵字:大腸桿菌、原子力顯微鏡、力與距離曲線、有效彈性常數、楊氏模數、紫外光、脂多醣層、肽聚醣層、細胞壁、表面增強拉曼光譜
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