我們設計以永久磁鐵為靜磁場B0的NMR核磁共振系統，在比較高的磁場(0.36 Tesla) 及較高的頻率(15.41 MHz)下量測自旋迴訊(spin-echo) 並計算出T2時間。由於磁鐵體積小磁場強，比以往的線圈節省空間。
本實驗架設源自於Pulsed NMR與Chip-NMR系統架構，系統架構包含永久磁鐵、功率放大器、脈波產生器、晶體震盪器、前級放大器、Pulse線圈、接收線圈、混頻器等。樣品體積為120µl，量測硫酸銅水溶液，不同濃度的磁性粒子，此外我們將Anti-CRP之抗體與奈米磁性粒子結合，形成一具有磁性生物標記的磁性試劑，再利用
spin -echo之量測結果觀察不同樣品T2的變化，期望此方法有機會運用在臨床醫學上。
未來更希望可以改進磁場均勻度，及製作屏蔽架構，期望可以使訊號品質更好。

[1] H.C. Yang, S.Y. Yang, G.L. Fang, W.H. Huang, C.H. Liu, S.H. Liao, H.E. Horng, C.Y. Hong, J. Appl. Phys. 99, 124701 (2006)
[2] K. Enpuku, T. Minotani, T. Gima, Y. Kuroki, Y. Itoh, M. Yamashita, Y. Katakura, S. Kuhara, Jpn. J. Appl. Phys. 38, L1102 (1999)
[3] C.Y. Hong, C.C. Wu, Y.C. Chiu, S.Y. Yang, H.E. Horng, H.C. Yang, Appl. Phys. Lett. 88, 212512 (2006)
[4] S. Appelt, F.W. Ha‥sing, S. Baer-Lang, N.J. Shah, B. Blümich,“Enhancement of Solution NMR and MRI with Laser-Polarized Xenon”, Chem.Phys. Lett. 348, 263
(2001)
[5] Hong-Chang Yang, Shu-Hsien Liao and Herng-Er Horng, and Shing-Ling Kuo, Hsin-Hsien Chen, and Shieh-Yueh Yang, “Enhancement of nuclear magnetic resonance in microtesla magneticfield with prepolarization field detected with high-Tc superconducting quantum interference device”, Appl. Phys. Lett. 88,
252505 (2006)
[6] Shu-Hsien Liao and Herng-Er Horng, Hong-Chang Yang, and Shieh-Yueh Yang, “Longitudinal relaxation time detection using a high-Tc superconductive quantum interference device magnetmeter”,J. Appl. Phys. 102, 033914 (2007).
[7] M.A. Espy, A.N. Matlachov, P.L. Volegov, J.C. Mosher, and R.H.Kraus Jr.,”SQUID-Based Simultaneous Detection of NMR and Biomagnetic Signals at Ultra-Low Magnetic Fields”, IEEE Trans.Appl. Supercon. 15, 635 (2005).
[8] A.H. Trabesinger, R. McDermott, S.K. Lee, M. Mu1ck, J. Clarke, and A. Pines , “ SQUID-Detected Liquid State NMR in Microtesla Fields“, J. Phys. Chem. A,108, 957-963 (2004).
[9] R. McDermott, S.K. Lee, B. ten Haken, A.H. Trabesinger, A. Pines, and J. Clarke, “Microtesla MRI with a superconducting quantum interference Device”, Proc. Natl. Acad. Sci. USA , 101, 7857 (2004).
[10] M. Mössle, S. Busch, M. Hatridge, W. Myers, A. Pines, and J. Clarke, “SQUID-detected microtesla MRI: a new modality for tumor detection”, paper presented at 2006 Applied Superconductivity conference, Aug. 27-Sept.1, 2006, Seattle, Washington, USA..
[11] H. C. Seton, D.M. Busell, J.S.M. Hutchison, I. Nicholson, D.J. Lurie,Phys. Med. Biol. 73, 2133 (1992).
[12] H. C. Seton, J.S.M. Hutchison, D. M. Busell, Meas. Sci. Technol. 8, 198 (1997).
[13] H. C. Seton, J.S.M. Hutchison, D. M. Busell, IEEE Trans. Appl. Supercon. 7,
3213 (1997).
[14] Hong-Chang Yang,Shu-Hsien Liao, Herng-Er Horng,Shing-Ling Kuo,Hsin-Hsien Chen, and S. Y. Yang, Appl. Phys. Lett. 88, 252505 (2006)
[15] S. Kumar, R. Mathews, S. G.. Haupt, D.K. Lathrop, M. Takigawa, J. R. Rozen, S. L. Brown, R. H. Koch, Appl. Phys. Lett. 70, 1037 (1997).
[16] S. Kumar, W. F. Avrin, B. R. Whitecotton, IEEE Trans. Magn. 32, 5261 (1996).
[17] K. Schlenga, R. F. McDemott, J. Clarke, R. E. de Souza, A. Wong-Foy, A. Pines, Appl. Phys. Lett. 75, 3695 (1999).
[18] N. Q. Fan, M. B. Heaney, J. Clarke, D. Newitt, L. L. Wald, E. L. Hahn, A. Bielecki, A. Pines, IEEE Trans. Magn 25, 1193 (1989).
[19] M. A. Espy, A. N. Matlachov, P. L. Volegov, J. C. Mosher, and R. H. Kraus, Jr. IEEE Trans. Appl. Supercon. 15, 635 (2005).
[20] M. Burghoff, S. Hartwig, L. Trahms, and J. Bernarding, Appl. Phys. Lett. 87,054103 (2005)
[21] M A Bernstein, K F King and X J Zhou. Handbook of MRI Pulse Sequences. Elsevier Academic Press, 960 (2004)
[22] E. L. Hahn: "Spin echoes" Phys. Rev 80,580-594 (1950)
[23] Pyrhonen, Juha; et al. Design of Rotating Electrical Machines. John Wiley & Sons. 2009: pp. 202.
[24]H. Y. Carr, E. M. Purcell: Effects of diffusion on free precession in nuclear magnetic resonance experiments. Phys Rev 94,630-638 (1954) Anything Ed Purcell signs his name to is worth reading! This certainly is one such example. A must for PNMR.
[25]N. Bloembergen, E. M. Purcell, and R. B. Pound, "Relaxation effects in Nuclear Magnetic Resonance absorption," Phys. Rev. 73, 679-712 (1948) A classic paper describing basic relaxation processes in NMR.
[26]S. Meiboom, D. Gill: Rev of Sci Instruments 29, 6881 (1958) The description of the phase shift technique that opened up multiple pulse

techniques to measuring very long T2's in liquids.
[27]K. Symon, "Mechanics" 3d ed. Addison-Wesley, Reading, MA (1971) A good place to learn about rotating coordinate systems, if you don't already understand them.
[28]R. G. Beaver, E. L. Hahn, Scientific American 6, 251 (1984) A discussion of the echo phenomenon and mechanical memory.
[29]Hakho Lee, Eric Sun, Donhee Ham & Ralph Weissleder. Chip–NMR biosensor for detection and molecular analysis of cells, Nat Med. 2008 Aug;14(8):869-74. doi: 10.1038/nm.1711. Epub 2008 Jul 6.