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研究生: 莊武璋
Wu-Chang Chuang
論文名稱: 吳茱萸藥材及製劑藥效成分之定量研究
Determination of the Bioactive Components in Evodia Rutaecarpa
指導教授: 許順吉
Xu, Shun-Ji
學位類別: 碩士
Master
系所名稱: 化學系
Department of Chemistry
畢業學年度: 81
語文別: 中文
論文頁數: 97
中文關鍵詞: 吳茱萸; 毛細電泳
英文關鍵詞: Evodia Rutaecarpa ; Capillary Electrophoresis(CE);
論文種類: 學術論文
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  • 吳茱萸為常用之中藥材,主要含有生物鹼、精油、有機酸及苦味素。藥理
    研究顯示生物鹼部分具有抗菌、鎮痛、強心、抗心律不整、保持體溫等作
    用。本研究以 Carboxy- evodiamine (Ⅱ),Dehydroevodiamine (Ⅲ),
    Evodiamine (Ⅳ),Rutaecarpine (Ⅴ),1-methyl-6-( )-6-undecenyl-
    4(1H)-quinolone (Ⅵ),Evocarpine (Ⅶ),Dihydro- evocarpine (Ⅷ)
    及一未定結構的化合物 (Ⅰ)等八個生物鹼為指標,以高效能液相層析儀
    來分析比較 24 件市售藥材之含量,並探討 4 種自製炮炙品之萃取量變
    化及其科學意義。分析結果顯示,市售品中各成分含量分別為 (Ⅰ)
    0.01 -9.23 mg/g,(Ⅱ) 0.01-13.86 mg/g,(Ⅲ) 16.26-38.07 mg/g,(
    Ⅳ) 0.2-18.84 mg/g,(Ⅴ) 0.56-8.93 mg/g,(Ⅵ) 0.88-16.60 mg/g,(
    Ⅶ) 0.08-5.45 mg/g,(Ⅷ) 0.01- 20.71 mg/g。整理歸納其外觀性狀與
    藥效成分之關係,發現以開口、氣味濃烈辛辣、色黑、粉碎後心皮多者藥
    效成分含量較高。炮炙結果顯示,適當的炮炙處裡皆能使藥效成分的萃取
    量增加,其中 Dehydroevodiamine 經加熱後會變成 Rutae- carpine 及
    一未知化合物 Unknown 2,比較四種炮炙方法發現用鹽水炒時
    Dehydroevodiamine 的轉換情形會受到抑制,其 Dehydroevodiamine/
    Rutaecarpine 之值,遠較其他炮炙品高。以 HPLC 定量市售含吳茱萸方
    劑時容易受到其他化合物干擾,改以 CE 定量時,可以定出較低含量的方
    劑之量。本研究以 HPLC 及 CE 同時定量市售吳茱萸湯、變製心氣飲、九
    檳吳茯湯及溫經湯中之 Dehydroevodiamine,並比較二種方法之差異及優
    劣。

    E. rutaecarpa is a widely used Chinese herbal drug which
    contains alkaloids, essential oils, carboxylic acids and
    limonoids as its main components. According to Pharmacological
    tests, the alkaloids were found to have antifungaling,
    analgesia, cardiotonic and body- temperature maintaining
    effects. In this research we used eight compounds as the
    reference standards to evaluate the quality of 24 commercial
    crude drugs and 4 processed drugs by means of HPLC. The
    scientific significance of herbal processing were also investi-
    gated. The analytical data showed that the contents of those
    commercial crude drugs were as follows: (Ⅰ) 0.01-9.23 mg/g,(
    Ⅱ) 0.01-13.86 mg/g,(Ⅲ) 16.26- 38.07 mg/g,(Ⅳ) 0.2-18.84 mg/g
    ,(Ⅴ) 0.56-8.93 mg/g,(Ⅵ) 0.88-16.60 mg/g,(Ⅶ) 0.01-5.45 mg/g
    ,(Ⅷ) 84 0.01-20.71 mg/g. Basically of contents of E. rutae-
    carpa were closely related to the appearance of the sample
    drugs. The contents of extractions were all increase after
    being processed by different processing methods and
    dehydroevodiamine would convert to rutaecarpine after being
    heated for some time. Those drugs which had been porcessed with
    salt showed a great variation in comparison with those which
    had been processed with vinegar and wine. It was thus believed
    that salt could stabilize the tetra-ammonium salt-
    Dehydroevodianine. In the presence of other components, the
    HPLC separation and qualitative determination of E. rutae-
    carpa containing formulas were easily interfered. CE technique
    was therefore introduced in our research and was found to
    enable some drug preparations which are low in content to be
    determined. In this research, both HPLC and CE methods were
    compared in the determi- nation of dehydroevodiamine in several
    commercial available Chinese herbal drug preparations.
    E. rutaecarpa is a widely used Chinese herbal drug which

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