藉由typeIb奈米鑽石粉末照射高能量離子束使其產生可在~700nm放出紅色的螢光的NV– 及NVO缺陷中心(defect center)，我們稱此鑽石為螢光奈米鑽石（fluorescent nanodiamond）。因為鑽石對生物沒有毒性、生物相容性高、且可輕易在表面做修飾反應，又經過雷射激發後鑽石內的缺陷中心所放出近紅外波段的螢光有比較好的組織穿透度，所以很適合運用於生物顯像上的研究。
　　所以本篇論文中，我們使用螢光奈米鑽石來做HeLa細胞的標記(cell label)，並且藉由流式細胞儀(flow cytometer)來對在Hela細胞體內的螢光奈米鑽石作定量分析並探討螢光奈米鑽石進入細胞之機制，然後透過細胞活性的測試，以證實製造出的螢光奈米鑽石對細胞有無毒性。此外再透過修飾螢光奈米鑽石的表面來攜帶運送DNA至細胞體內並觀察分布狀態。為了要達成對多細胞的生物有機體活體顯像之目的，我們採取餵食線蟲的方式來標記其消化系統及注射至線蟲之生殖腺(gonad)再經由生殖的過程(oogenesis)來標記其胚胎。

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