研究生: |
何晉容 Ho, Ching-Jung |
---|---|
論文名稱: |
深層海水口服與外用對於二硝基氯苯誘導之異位性皮膚炎BALB/c小鼠模式的療效 Therapeutic Effects of Oral Administration and Topical Application of Deep-Ocean-Water on DNCB-induced Atopic Dermatitis in BALB/c Mice |
指導教授: |
鄭劍廷
Chien, Chiang-Ting |
學位類別: |
碩士 Master |
系所名稱: |
生命科學系 Department of Life Science |
論文出版年: | 2017 |
畢業學年度: | 105 |
語文別: | 中文 |
論文頁數: | 75 |
中文關鍵詞: | 皮膚炎 、深層海水 、二硝基氯苯 、脾臟腫大 |
英文關鍵詞: | Dermatitis, Deep ocean water, DNCB, Splenomegaly |
DOI URL: | https://doi.org/10.6345/NTNU202202339 |
論文種類: | 學術論文 |
相關次數: | 點閱:112 下載:7 |
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異位性皮膚炎是一種慢性的、發炎性皮膚疾病,其病症特徵有持續性的發炎反應和有搔癢感的皮膚損傷。本研究會在BALB/c小鼠的背部皮膚上持續地使用DNCB誘發類似異位性皮膚炎病症和傷害,並且評估使用深層海水對於由DNCB處理所誘發的類似異位性皮膚炎症狀及皮膚傷害是否具有改善的效果。深層海水富含營養物質和礦物鹽,並有著抗氧化和抗發炎的特性,或許可以做為發展成新的治療方式來為皮膚提供保護,抵抗異位性皮膚炎。因為深層海水獨特的特性,在現在的研究中,認為深層海水對於DNCB誘發之類似異位性皮膚炎症狀或許有治療的潛力。本研究的結果顯示在DNCB處理的口服深層海水組別,其表皮分數和肥大細胞數量有獲得改善的效果,而且在皮下血管白血球細胞浸潤以及脾臟形態的情形有緩和的趨勢。然而,在抓癢行為、表皮增厚、脾臟腫大以及IL-4含量方面並沒有顯著性的改善。DNCB處理的口服與外用深層海水萃取物的組別,其表皮分數、表皮增厚、肥大細胞數量和IL-4含量有獲得改善的效果,而且在皮下血管白血球細胞浸潤以及脾臟形態的情形有緩和的趨勢。然而,在抓癢行為、脾臟腫大方面並沒有顯著性的改善。結論,深層海水或許在改善異位性皮膚炎是一個有潛力的治療策略,其中又以同時口服深層海水以及外用深層海水萃取物抹劑的效果最好。
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized with continuous inflammation and pruritic skin lesions. Repeated application of 2,4-dinitrochlorobenzene (DNCB) treated on the dorsal skin of BALB/c mice can induce AD-like symptoms and skin lesions. This study evaluated therapeutic effects of oral administration or oral combined topical application of deep ocean water (DOW) on DNCB-induced AD-like symptoms. DOW which is rich in micronutrients and minerals and with antioxidant and anti-inflammatory qualities, may be developed as an AD therapy to provide skin protection against AD. Because of the unique property of DOW, in recent studies, suggesting that its therapeutic potential on DNCB-induced AD-like symptoms in BALB/c mice.
The present results showed that dermatitis score and number of mast cells was significantly alleviated in DNCB-treated oral administration groups, and infiltration of leukocytes and morphological changes in spleen were slightly attenuated. However, there was no significant difference in scratching behavior, epidermal thickness, splenomegaly and spleen IL-4 levels. In DNCB-treated oral administration and topical application groups, dermatitis score, epidermal thickness, number of mast cells and spleen IL-4 levels was significantly alleviated, and infiltration of leukocytes and morphological changes in spleen were slightly attenuated. However, there was no significant difference in scratching behavior and splenomegaly. In conclusion, these results suggest that DOW treatment may be a potentially therapeutic strategy in ameliorating atopic dermatitis. Furthermore, combination of oral administration and topical application of DOW had the best efficiency to ameliorate AD-like symptoms.
1. Hwang, C. Y., Chen, Y. J., Lin, M. W., Chen, T. J., Chu, S. Y., Chen, C. C., Lee, D. D., Chang, Y. T., Wang, W. J., and Liu, H. N., Prevalence of atopic dermatitis, allergic rhinitis and asthma in Taiwan: a national study 2000 to 2007. Acta Derm Venereol, 2010. 90(6): p. 589-94.
2. Leung, D. Y., Atopic dermatitis: immunobiology and treatment with immune modulators. Clin Exp Immunol, 1997. 107 Suppl 1: p. 25-30.
3. Bieber, T., Atopic dermatitis. N Engl J Med, 2008. 358(14): p. 1483-94.
4. Chen, L., Martinez, O., Overbergh, L., Mathieu, C., Prabhakar, B. S., and Chan, L. S., Early up-regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model. Clin Exp Immunol, 2004. 138(3): p. 375-87.
5. Leung, D. Y., Boguniewicz, M., Howell, M. D., Nomura, I., and Hamid, Q. A., New insights into atopic dermatitis. J Clin Invest, 2004. 113(5): p. 651-7.
6. Palmer, C. N., Irvine, A. D., Terron-Kwiatkowski, A., Zhao, Y., Liao, H., Lee, S. P., Goudie, D. R., Sandilands, A., Campbell, L. E., Smith, F. J., O'Regan, G. M., Watson, R. M., Cecil, J. E., Bale, S. J., Compton, J. G., DiGiovanna, J. J., Fleckman, P., Lewis-Jones, S., Arseculeratne, G., Sergeant, A., Munro, C. S., El Houate, B., McElreavey, K., Halkjaer, L. B., Bisgaard, H., Mukhopadhyay, S., and McLean, W. H., Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat Genet, 2006. 38(4): p. 441-6.
7. Eichenfield, L. F., Tom, W. L., Chamlin, S. L., Feldman, S. R., Hanifin, J. M., Simpson, E. L., Berger, T. G., Bergman, J. N., Cohen, D. E., Cooper, K. D., Cordoro, K. M., Davis, D. M., Krol, A., Margolis, D. J., Paller, A. S., Schwarzenberger, K., Silverman, R. A., Williams, H. C., Elmets, C. A., Block, J., Harrod, C. G., Smith Begolka, W., and Sidbury, R., Guidelines of care for the management of atopic dermatitis: section 1. Diagnosis and assessment of atopic dermatitis. J Am Acad Dermatol, 2014. 70(2): p. 338-51.
8. Cury Martins, J., Martins, C., Aoki, V., Gois, A. F., Ishii, H. A., and da Silva, E. M., Topical tacrolimus for atopic dermatitis. Cochrane Database Syst Rev, 2015(7): p. CD009864.
9. Correa da Rosa, J., Malajian, D., Shemer, A., Rozenblit, M., Dhingra, N., Czarnowicki, T., Khattri, S., Ungar, B., Finney, R., Xu, H., Zheng, X., Estrada, Y. D., Peng, X., Suarez-Farinas, M., Krueger, J. G., and Guttman-Yassky, E., Patients with atopic dermatitis have attenuated and distinct contact hypersensitivity responses to common allergens in skin. J Allergy Clin Immunol, 2015. 135(3): p. 712-20.
10. Tamagawa-Mineoka, R., Masuda, K., Ueda, S., Nakamura, N., Hotta, E., Hattori, J., Minamiyama, R., Yamazaki, A., and Katoh, N., Contact sensitivity in patients with recalcitrant atopic dermatitis. J Dermatol, 2015. 42(7): p. 720-2.
11. Wong, V. K., Della Croce, C., Schonfeld, S., Mastrangelo, A. M., and Lebwohl, M., Use and abuse of topical corticosteroids in infections of the skin and related structures. J Drugs Dermatol, 2003. 2(3): p. 268-76.
12. Hon, K. L., Leung, T. F., Ng, P. C., Lam, M. C., Kam, W. Y., Wong, K. Y., Lee, K. C., Sung, Y. T., Cheng, K. F., Fok, T. F., Fung, K. P., and Leung, P. C., Efficacy and tolerability of a Chinese herbal medicine concoction for treatment of atopic dermatitis: a randomized, double-blind, placebo-controlled study. Br J Dermatol, 2007. 157(2): p. 357-63.
13. Amestejani, M., Salehi, B. S., Vasigh, M., Sobhkhiz, A., Karami, M., Alinia, H., Kamrava, S. K., Shamspour, N., Ghalehbaghi, B., and Behzadi, A. H., Vitamin D supplementation in the treatment of atopic dermatitis: a clinical trial study. J Drugs Dermatol, 2012. 11(3): p. 327-30.
14. Bjorneboe, A., Soyland, E., Bjorneboe, G. E., Rajka, G., and Drevon, C. A., Effect of dietary supplementation with eicosapentaenoic acid in the treatment of atopic dermatitis. Br J Dermatol, 1987. 117(4): p. 463-9.
15. de Andrade, S. C., de Carvalho, R. F., Soares, A. S., de Abreu Freitas, R. P., de Medeiros Guerra, L. M., and Vilar, M. J., Thalassotherapy for fibromyalgia: a randomized controlled trial comparing aquatic exercises in sea water and water pool. Rheumatol Int, 2008. 29(2): p. 147-52.
16. Katsuda, S., Yasukawa, T., Nakagawa, K., Miyake, M., Yamasaki, M., Katahira, K., Mohri, M., Shimizu, T., and Hazama, A., Deep-sea water improves cardiovascular hemodynamics in Kurosawa and Kusanagi-Hypercholesterolemic (KHC) rabbits. Biol Pharm Bull, 2008. 31(1): p. 38-44.
17. Hwang, H. S., Kim, H. A., Lee, S. H., and Yun, J. W., Anti-obesity and antidiabetic effects of deep sea water on ob/ob mice. Mar Biotechnol (NY), 2009. 11(4): p. 531-9.
18. Hwang, H. S., Kim, S. H., Yoo, Y. G., Chu, Y. S., Shon, Y. H., Nam, K. S., and Yun, J. W., Inhibitory effect of deep-sea water on differentiation of 3T3-L1 adipocytes. Mar Biotechnol (NY), 2009. 11(2): p. 161-8.
19. Yokota, J., Kitaoka, T., Jobu, K., Takuma, D., Hamada, A., Onogawa, M., Yoshioka, S., Kyotani, S., and Miyamura, M., Eriobotrya japonica seed extract and deep sea water protect against indomethacin-induced gastric mucosal injury in rats. J Nat Med, 2011. 65(1): p. 9-17.
20. asri, H., Helicobacter pylori infection and its relationship to plasma magnesium in hemodialysis patients. Bratisl Lek Listy, 2007. 108(12): p. 506-9.
21. Yang, C. C., Yao, C. A., Lin, Y. R., Yang, J. C., and Chien, C. T., Deep-sea water containing selenium provides intestinal protection against duodenal ulcers through the upregulation of Bcl-2 and thioredoxin reductase 1. PLoS One, 2014. 9(7): p. e96006.
22. Hataguchi, Y., Tai, H., Nakajima, H., and Kimata, H., Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome. Eur J Clin Nutr, 2005. 59(9): p. 1093-6.
23. Kimata, H., Tai, H., Nakagawa, K., Yokoyama, Y., Nakajima, H., and Ikegami, Y., Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patients with atopic eczema/dermatitis syndrome (AEDS). Acta Medica (Hradec Kralove), 2002. 45(2): p. 83-4.
24. Proksch, E., Nissen, H. P., Bremgartner, M., and Urquhart, C., Bathing in a magnesium-rich Dead Sea salt solution improves skin barrier function, enhances skin hydration, and reduces inflammation in atopic dry skin. Int J Dermatol, 2005. 44(2): p. 151-7.
25. Bak, J. P., Kim, Y. M., Son, J., Kim, C. J., and Kim, E. H., Application of concentrated deep sea water inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice. BMC Complement Altern Med, 2012. 12: p. 108.
26. Radhakrishnan, G., Yamamoto, M., Maeda, H., Nakagawa, A., KatareGopalrao, R., Okada, H., Nishimori, H., Wariishi, S., Toda, E., Ogawa, H., and Sasaguri, S., Intake of dissolved organic matter from deep seawater inhibits atherosclerosis progression. Biochem Biophys Res Commun, 2009. 387(1): p. 25-30.
27. Fu, Z. Y., Yang, F. L., Hsu, H. W., and Lu, Y. F., Drinking deep seawater decreases serum total and low-density lipoprotein-cholesterol in hypercholesterolemic subjects. J Med Food, 2012. 15(6): p. 535-41.
28. Kwon, H. K., Lee, C. G., So, J. S., Chae, C. S., Hwang, J. S., Sahoo, A., Nam, J. H., Rhee, J. H., Hwang, K. C., and Im, S. H., Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders. Proc Natl Acad Sci U S A, 2010. 107(5): p. 2159-64.
29. Han, H. M., Kim, S. J., Kim, J. S., Kim, B. H., Lee, H. W., Lee, Y. T., and Kang, K. H., Ameliorative effects of Artemisia argyi Folium extract on 2,4dinitrochlorobenzeneinduced atopic dermatitislike lesions in BALB/c mice. Mol Med Rep, 2016. 14(4): p. 3206-14.
30. Leung, D. Y., Hirsch, R. L., Schneider, L., Moody, C., Takaoka, R., Li, S. H., Meyerson, L. A., Mariam, S. G., Goldstein, G., and Hanifin, J. M., Thymopentin therapy reduces the clinical severity of atopic dermatitis. J Allergy Clin Immunol, 1990. 85(5): p. 927-33.
31. Takano, N., Arai, I., and Kurachi, M., Analysis of the spontaneous scratching behavior by NC/Nga mice: a possible approach to evaluate antipruritics for subjects with atopic dermatitis. Eur J Pharmacol, 2003. 471(3): p. 223-8.
32. Leung, D. Y. and Bieber, T., Atopic dermatitis. Lancet, 2003. 361(9352): p. 151-60.
33. Brandt, E. B. and Sivaprasad, U., Th2 Cytokines and Atopic Dermatitis. J Clin Cell Immunol, 2011. 2(3).
34. Baldo, A., Cafiero, M., Di Caterino, P., and Di Costanzo, L., Tacrolimus ointment in the management of atopic dermatitis. Clin Cosmet Investig Dermatol, 2009. 2: p. 1-7.
35. Sohn, E. H., Jang, S. A., Lee, C. H., Jang, K. H., Kang, S. C., Park, H. J., and Pyo, S., Effects of korean red ginseng extract for the treatment of atopic dermatitis-like skin lesions in mice. J Ginseng Res, 2011. 35(4): p. 479-86.
36. Sur, B., Lee, B., Yoon, Y. S., Lim, P., Hong, R., Yeom, M., Lee, H. S., Park, H., Shim, I., Lee, H., Jang, Y. P., and Hahm, D. H., Extract of Polygala tenuifolia Alleviates Stress-Exacerbated Atopy-Like Skin Dermatitis through the Modulation of Protein Kinase A and p38 Mitogen-Activated Protein Kinase Signaling Pathway. Int J Mol Sci, 2017. 18(1).
37. Olsson, M., Broberg, A., Jernas, M., Carlsson, L., Rudemo, M., Suurkula, M., Svensson, P. A., and Benson, M., Increased expression of aquaporin 3 in atopic eczema. Allergy, 2006. 61(9): p. 1132-7.
38. Weidinger, S. and Novak, N., Atopic dermatitis. Lancet, 2016. 387(10023): p. 1109-1122.
39. Lee, S. H., Heo, Y., and Kim, Y. C., Effect of German chamomile oil application on alleviating atopic dermatitis-like immune alterations in mice. J Vet Sci, 2010. 11(1): p. 35-41.
40. Dang, L., He, L., Wang, Y., Xiong, J., Bai, B., and Li, Y., Role of the complement anaphylatoxin C5a-receptor pathway in atopic dermatitis in mice. Mol Med Rep, 2015. 11(6): p. 4183-9.
41. Kawakami, T., Ando, T., Kimura, M., Wilson, B. S., and Kawakami, Y., Mast cells in atopic dermatitis. Curr Opin Immunol, 2009. 21(6): p. 666-78.
42. Chapman, J. and Bhimji, S., Splenomegaly, in StatPearls. 2017: Treasure Island (FL).
43. Kim, C. G., Kang, M., Lee, Y. H., Min, W. G., Kim, Y. H., Kang, S. J., Song, C. H., Park, S. J., Park, J. H., Han, C. H., Lee, Y. J., and Ku, S. K., Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice. Evid Based Complement Alternat Med, 2015. 2015: p. 179185.